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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: bodhisatta]
#22247416 - 09/16/15 06:40 PM (8 years, 7 months ago) |
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I'd say that's fairly conclusive that it's bacteria and no mold infection.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,867
Loc: Canada
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: bodhisatta]
#22247420 - 09/16/15 06:42 PM (8 years, 7 months ago) |
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Really would like to know what kind.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: Pastywhyte]
#22247436 - 09/16/15 06:46 PM (8 years, 7 months ago) |
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interestingly enough since it's hard to captivate what I'm actually seeing but some of the rod shaped bacteria are motile but the large majority of them are not motile (or seem so) and very short. some of them are so short they almost look like diplococci maybe lactococcus since they are some times elongated a bit.
the swimmers my guess is Pseudomonas fluorescens
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: bodhisatta]
#22277637 - 09/23/15 07:18 AM (8 years, 7 months ago) |
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 I would call this problem very contagious
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,867
Loc: Canada
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: bodhisatta]
#22277658 - 09/23/15 07:28 AM (8 years, 7 months ago) |
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That is definitely pseudomonias. But I see no necrosis of the stipe on those fruits. Hmmm.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: Pastywhyte]
#22811497 - 01/22/16 03:07 PM (8 years, 3 months ago) |
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edited the op
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Thedillestpickle
cultured



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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: bodhisatta]
#24026171 - 01/20/17 04:33 PM (7 years, 3 months ago) |
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So you concluded that the contamination was from a bacteria and not verticillium.
Would you say that these mushrooms also have the same contamination? There are many posts are shroomery that look to have a similar contamination and everyone is calling it verticllium. This thread has something that looks similar but maybe just a look-a-like or maybe this thread is actually proving that what everyone thought was verticillium was really bacteria all along.
Any opinions on what I have here?
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Pastywhyte
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Registered: 09/15/12
Posts: 37,867
Loc: Canada
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: Thedillestpickle]
#24026181 - 01/20/17 04:36 PM (7 years, 3 months ago) |
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Yeah I would say those were bacteria. Lencacillium (verticillium) is better charecterized by the peeling and splitting stipe, blown out and slumped fruits, with greyish caps.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: Pastywhyte]
#24026483 - 01/20/17 06:40 PM (7 years, 3 months ago) |
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yep bacteria, yours look like some bacteria too, and what pasty said
air and light can be in more abundance
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: bodhisatta]
#27424776 - 08/11/21 11:47 PM (2 years, 9 months ago) |
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Quote:
bodhisatta said: I would call this problem very contagious
How did you end up dealing with this? Did you need to shut the lab down and clean, or something less drastic?
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Psilimax
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: gone-pear-shaped]
#27425241 - 08/12/21 11:52 AM (2 years, 9 months ago) |
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Sounds like a mulligan. I get that shit too ! Plan : clean ,wipe, scrub ,rinse , disinfectant repeat and blast with germicidal light while the labs unoccupied. ( shielding any live mycelium or spores) A dehumidifier may help too as water is life & If the air is dry there’s less crud that can live in it . I found the brown streak was from overly humid conditions but it sounds much more complicated than that. I never worried about a little bit , and it hasn’t made me ill . Though I discard the ones that have more than a little spot or streak . I pray it’s benign. I harvest early and it helps plus I like closed caps and partially open ones better . I seems to me if I physically abate it , it makes a difference and doesn’t spread .
-------------------- “I would never trust an organization that would have me as a member .” - Groucho Marx -
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: gone-pear-shaped]
#27426419 - 08/13/21 07:08 AM (2 years, 9 months ago) |
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Quote:
gone-pear-shaped said:
Quote:
bodhisatta said: I would call this problem very contagious
How did you end up dealing with this? Did you need to shut the lab down and clean, or something less drastic?
Just start over. Go back to petri dishes etc..
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Psilimax
Bad hair


Registered: 08/01/21
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Re: verticillium or bacteria, microscopic examination of brown spots on stems [Re: bodhisatta]
#27428193 - 08/14/21 04:04 PM (2 years, 8 months ago) |
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Is this the offender? The brown streak strikes again. It’s a second flush of Cambodian from a MS BRF cake.
-------------------- “I would never trust an organization that would have me as a member .” - Groucho Marx -
Edited by Psilimax (08/14/21 04:07 PM)
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