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OfflinePussyFart
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Re: Here's a great fruiting tray: [Re: mrbart4444]
    #22160271 - 08/29/15 11:19 AM (8 years, 5 months ago)

Slower, but not needed...lol.


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Offlinespacechildo
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Re: Here's a great fruiting tray: [Re: PussyFart]
    #22160283 - 08/29/15 11:22 AM (8 years, 5 months ago)

slow and steady always wins the race IME. its easy to rush into failure.


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: Here's a great fruiting tray: [Re: PussyFart]
    #22160286 - 08/29/15 11:22 AM (8 years, 5 months ago)

chart i use for reference 


winter time i have no choice but to incubate though for sure my house stays 65-70 and takes like 5 weeks for what wouldve been 2 1/2


--------------------


Edited by mrbart4444 (08/29/15 11:23 AM)


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InvisibleNumeroEno
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Re: Here's a great fruiting tray: [Re: PussyFart]
    #22160293 - 08/29/15 11:23 AM (8 years, 5 months ago)

Quote:

PussyFart said:
U guys keep saying incubation when u mean colonization....nobody should need to incubate anything....unless temps are way below room temp...




I always used the terms interchangeably. I'm of the mind that incubation can be done without applying heat, but I could be wrong. Either way it's just semantics to me :shrug:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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OfflinePussyFart
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Re: Here's a great fruiting tray: [Re: NumeroEno]
    #22160307 - 08/29/15 11:26 AM (8 years, 5 months ago)

Quote:

mrbart4444 said:
chart i use for reference 


winter time i have no choice but to incubate though for sure my house stays 65-70 and takes like 5 weeks for what wouldve been 2 1/2



Anything above 80F promotes too much contam growth for me.....and for most on this forum....plus it slows the mycellium down...

If the outside of the jars are 86F, then the inside is 90+ easily....no ty....

Quote:

NumeroEno said:
Quote:

PussyFart said:
U guys keep saying incubation when u mean colonization....nobody should need to incubate anything....unless temps are way below room temp...




I always used the terms interchangeably. I'm of the mind that incubation can be done without applying heat, but I could be wrong. Either way it's just semantics to me :shrug:



Incubation means applying heat...that is all.....colonization means something completely different....They cannot be used interchangeably....it's english, not semantics....


Edited by PussyFart (08/29/15 11:26 AM)


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Offlinespacechildo
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Re: Here's a great fruiting tray: [Re: PussyFart]
    #22160334 - 08/29/15 11:31 AM (8 years, 5 months ago)

that chart must be based off what stamets put in his book. no one sees fast cubensis mycelium growth at 86F thats crazy.


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InvisibleNumeroEno
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Re: Here's a great fruiting tray: [Re: PussyFart]
    #22160358 - 08/29/15 11:36 AM (8 years, 5 months ago)

Not to be pedantic or anything, but Merriam Webster says:

Quote:

b :to maintain (as an embryo or a chemically active system) under conditions favorable for hatching, development, or reaction




I see no mention of heat, and I have no intention of arguing the point further at risk of going off-topic, but I am not wrong.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


Edited by NumeroEno (08/29/15 11:38 AM)


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OfflinePussyFart
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Re: Here's a great fruiting tray: [Re: NumeroEno]
    #22160378 - 08/29/15 11:40 AM (8 years, 5 months ago)

But it's not the action of development, it's just the maintenance of keeping it under certain conditions for development......

The actual development is called colonization.

I am not wrong.

The only thing incubation means(in this context) is applying heat to the culture.....it has no other meaning....we cannot do anything else to it except apply or remove heat....


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InvisibleNumeroEno
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Re: Here's a great fruiting tray: [Re: PussyFart]
    #22160457 - 08/29/15 11:58 AM (8 years, 5 months ago)

I don't think either of us are wrong. I just think it is a term with a very broad definition. You could easily "incubate" your jars just by keeping them in a slightly warmer room, but OTOH I do agree that leaving them on the shelf to do their own thing isn't incubating them per se.

It's definitely one of the more subjective aspects of cultivation, as well as a term where a lot of different things could reasonably be considered incubating. In the end though, for all intents and purposes pertaining to our hobby, it means keeping your culture at a warmer temp to favor faster colonization, and I think we can both agree on that :cool:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Invisibletahoe
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Re: Here's a great fruiting tray: [Re: NumeroEno]
    #22161161 - 08/29/15 03:06 PM (8 years, 5 months ago)

I use the rubbermaid dishwashing tubs. They cost about two bucks, they are dark, and clean easily. Cat litter boxes work well also.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

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I need to proofread


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OfflineThriftyNickel
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Re: Here's a great fruiting tray: [Re: tahoe]
    #22162161 - 08/29/15 07:28 PM (8 years, 4 months ago)



I spawned these trays with grain this morning, and put plastic wrap with holes on one, so it will be experiment. I'll let ya'll know in a few weeks. I haven't been making an effort to grow in the dark, Its that I have NOT been making an effort to grow in the light. It seems as though mycellium grows under the surface of things where there is no light, so it makes sense to me. If anyone as any good references to past experiments or published material on this can you please send a link?


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OfflineThriftyNickel
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Re: Here's a great fruiting tray: [Re: PussyFart]
    #22162226 - 08/29/15 07:45 PM (8 years, 4 months ago)

Quote:

ThrifyNickel said:
mycellium appeared uniformly over the grain



This does not matter.....you cannot tell if its a monoculture in a jar, only a dish with a 2d surface....





I described the uniformity so you guys can get a visual and possibly point out if anything sounds unusual. I've been a little concerned lately that the mycellium doesn't look very rhizomorphic sometimes. It never occurred to me until after reading from Stamets that it should be a sign of good health and vigor. I don't know if it matters or not. I was really more concerned with trying to make a point that the mycellium grew just fine without light, in my opinion, and tried to leave it open for someone to call me out on it.


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InvisibleMad Season
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Re: Here's a great fruiting tray: [Re: ThriftyNickel]
    #22162268 - 08/29/15 07:57 PM (8 years, 4 months ago)

Quote:

RogerRabbit said:
Quote:

Diesel_Dawg said:
I've never done a back to back of light vs dark.. But I have done direct vs indirect, and the direct light significantly slowed growth.




This is correct.  You want low level light during colonization. 

I've repeated this experiment a few thousand times now.  The colonizing bags in our mushroom farm near the north facing window grow at least 1/3 faster than those in the areas where it's darker.  Also, substrates colonizing near the isle where light penetrates colonize faster.

With most species you can inoculate agar and then stack two dishes on top of each other.  The top one will nearly always colonize faster than the bottom one.

What you don't want is very bright light during colonization which not only slows down growth but also could lead to premature fruiting.
RR



I searched light during colonization and clicked the 2nd link :shrug:

Also agar is the best way of actually figuring it out. Jars won't tell you enough.
http://www.shroomery.org/forums/showflat.php/Number/18430998


--------------------
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Edited by Mad Season (08/29/15 08:00 PM)


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OfflineMachiavelliavore
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Re: Here's a great fruiting tray: [Re: ThriftyNickel]
    #22163223 - 08/30/15 01:18 AM (8 years, 4 months ago)

Haha, I've been thinking about fruiting an almost identical setup (including the straw) in a greenhouse as well.

The container I found was a 40qt hefty measuring 16"x23"x6"  The contain has smooth edges, grey lid w clear container, and costs $10 at target.

How much did that container cost?  If it's about the same, I think the hefty would be better.  Love the idea of the space efficieny and ease of lining and straw packing with these megatrays.

I've been fruiting with 4500K light, and even without any light with decent success.  Great pinsets, some meh dry yields.  Might be the heat.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (08/30/15 01:20 AM)


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OfflineThriftyNickel
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Re: Here's a great fruiting tray: [Re: Machiavelliavore]
    #22239521 - 09/15/15 01:45 AM (8 years, 4 months ago)

I checked my grow room tonight and here are the results: I lost two bins to trich after they were cased (my first time casing, not sure where I went wrong). On Sept 4th, I inoculated 7 jars of WBS with spawn from one strain (I believe) in a Petri dish. 4 of those were left inside a black garbage bag on a rack and 3 were set beside and exposed to 12 hrs 6000k light a day. I'm not sure if they colonized at different rates, but both control groups are 100 percent colonized now. The dark lid straw bin appears nearly fully colonized, and the bin with the plastic wrap lid (with air holes) isn't yet colonized. The room air temperature was set at 80, but usually fluctuates 79-82. The bin that received light was 12" off the floor, and the dark one was 24" off the floor. I'm surprised to see the lack of growth in this one, perhaps it's a sign of something wrong. I don't see how light would negatively affect things. However, this seems to prove that light isn't necessary for normal mycelium growth.

http://

http://

http://


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OfflineThriftyNickel
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Re: Here's a great fruiting tray: [Re: Mad Season]
    #22239560 - 09/15/15 02:23 AM (8 years, 4 months ago)

Thanks for the link mad season


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