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Invisibleskuzzy1232
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Registered: 05/31/10
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Contamination problem help, 25 bags 20 contaminated me or the sponsor ?????
    #22155969 - 08/28/15 10:55 AM (8 years, 5 months ago)

Ok so I got syringes from a shroomery sponsor one of the best.

I soaked Wbs for 9-10 hours , washed with fresh water , dried and loaded into bags and pressure cooked at 15 psi for an hour and 30 minutes in this thing

Then they were allowed to cool over night and I moved them here
I bleached and knocked up each bag . I put duct tape over the injection hole.

I used about three different syringes PER strain so 3 for two , and two syringes for one strain.
To test my flow hood, I knocked up a case of jars with a new syringes from the same place...
It has been for days no growth or  contamination ,
I have done four transfers
The first is doing great !

Then I only have one out of three contaminations from my transfers which I expected because I am cleaning the wild strain.





The jars are 4 days old and not doing anything
I lost 20 bags to contaminations . Trich on three, a powderd yellow mold on most others and a few with cobweb what the fuck did I do wrong????


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http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .


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OfflinetaGyo
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: skuzzy1232]
    #22155989 - 08/28/15 10:59 AM (8 years, 5 months ago)

You have a few variables that have appeared to change since your last go around.

Check that that pressure cooker is reaching 15 PSI and maintaining it for the whole hour and thirty minutes. I'm sure you've tested but we have to cross that out.
Did you use agar with any of the syringes before inoculating? I think it's the syringes honestly.

I would run a control. Sterilize two spawn bags, one using your old method and the other using this new method. Make sure you get clean spawn on agar first and then knock them up with LI or something of the sort (Liquids do really well in bags).

Test those needles on agar, they're probably contamed.


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Gyo's Better Grows
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InvisibleMad Season
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: skuzzy1232]
    #22156028 - 08/28/15 11:07 AM (8 years, 5 months ago)

It looks like he is using agar. Apparently on his 4th transfer. I didn't really understand the agar part honestly. I'd like more info on that. Are you nocing the bags with clean liquid from the agar, and tested the liquid again on agar?


Edited by Mad Season (08/28/15 11:08 AM)


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OfflinetaGyo
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: Mad Season]
    #22156038 - 08/28/15 11:09 AM (8 years, 5 months ago)

Yeah I see he's using agar but honestly I can't make heads or tails of what that is. I think the bottom two pics are a wild strain?

My point being he didn't put any of his vendor bought needles on agar.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum


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InvisibleMad Season
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: taGyo]
    #22156056 - 08/28/15 11:12 AM (8 years, 5 months ago)

Lol :shrug: I really don't understand the agar part. So I'll say sure lol. He does mention wild soooo... maybe that's all he's doing lol


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
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OfflinetaGyo
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: Mad Season]
    #22156069 - 08/28/15 11:14 AM (8 years, 5 months ago)

Idk it looks like OP was just like "PICTURES WILL EXPLAIN EVERYTHING." and threw in words as an afterthought :lmafo:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum


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Invisibleskuzzy1232
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: taGyo]
    #22156084 - 08/28/15 11:18 AM (8 years, 5 months ago)

Ok sorry . I will clarify

All the agar is the wild strain. My personal. I am on my second transfer:))))) I was using the agar to test my flow hood which I think checks out.

The vendors syringes were all used for the bags.
As far as the skuzzy unit #1 aka my giant pressure cooker, the gauge reads 15 before I start time...the pressure weight is an aa pressure cooker weight and stem..
I am running a controlled now I have these jars.prepped the same except in the smaller AA


So far 5 days three strains no growth..


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .


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OfflinetaGyo
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: skuzzy1232]
    #22156090 - 08/28/15 11:19 AM (8 years, 5 months ago)

Test your needles on your agar :thumbup:. Honestly if you're working with agar you should never do vendor syringe to jars.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Invisibleskuzzy1232
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: skuzzy1232]
    #22156095 - 08/28/15 11:20 AM (8 years, 5 months ago)

And I smoked weed before posting so yeah...


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .


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Invisibleskuzzy1232
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: taGyo]
    #22156117 - 08/28/15 11:23 AM (8 years, 5 months ago)

Quote:

taGyo said:
Test your needles on your agar :thumbup:. Honestly if you're working with agar you should never do vendor syringe to jars.



The whole point of going through a vendor is so that it saves me work. If I wanted to clean everything up on agar all the time and spend a lot of time I would just use the market here on the shroomery .


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .


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InvisibleMad Season
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: skuzzy1232]
    #22156133 - 08/28/15 11:26 AM (8 years, 5 months ago)

Then do that. No spores are sterile. They came from open air. All protects have to be started on agar to ensure success. Otherwise you'll be like a dog chasing its tail trying to figure out where your contams are. If you don't start clean.. you're not doing it right haha


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinetaGyo
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: skuzzy1232]
    #22156146 - 08/28/15 11:27 AM (8 years, 5 months ago)

Vendors make mistakes too :shrug:

I've had 8 APE syringes. All have some sort of contam.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Invisibleskuzzy1232
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: Mad Season]
    #22156171 - 08/28/15 11:32 AM (8 years, 5 months ago)

Quote:

Mad Season said:
Then do that. No spores are sterile. They came from open air. All protects have to be started on agar to ensure success. Otherwise you'll be like a dog chasing its tail trying to figure out where your contams are. If you don't start clean.. you're not doing it right haha




True ... Never had a total failure before though ...

I will test on agar I orderd 25 syringes

The agar mycelium came from this,

I have been working with it for about a year it eats small mold patches.


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .


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Invisibletahoe
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: skuzzy1232]
    #22156239 - 08/28/15 11:43 AM (8 years, 5 months ago)

What size bags or jars did you pc for 90 mins? How many cups of seed were in each one?
When I use a spore syringe or lc I will not shake the bag until I see growth.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
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OfflineAppalachian Brony
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: Mad Season]
    #22156248 - 08/28/15 11:45 AM (8 years, 5 months ago)

Yeah spore syringes aren't perfect. Idk how big your bags were, but they're generally holding at least 4x the spawn of quarts and that seems like it would take like 2 months from spores to colonized.

Agar saves me about a week from spores to colonized. I only went syringe to grain once and never again. RGS germinates in maybe a week, but the rye jars I did took like 2 fuckin weeks to germinate. Then you gotta wait at least 2 weeks for colonization.

On agar I get germination in under a week, make a transfer, and in a few days I can inoculate and just wait for colonization.


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InvisibleMad Season
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: skuzzy1232]
    #22156263 - 08/28/15 11:47 AM (8 years, 5 months ago)

It always happens when you're testing new equipment right? :rofl: I've had an even worse failure lol. 1/5 success rate is better than my 0 success rate once. Also was the turning point of me starting agar lol.

Agreed with tahoe. What size bags for only 90 minutes? Honestly I do qt jars for 2 hours. 4+ qt bags for 3.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinestreamer

Registered: 09/06/14
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: Appalachian Brony]
    #22156291 - 08/28/15 11:55 AM (8 years, 5 months ago)

That sucks OP, I also expected clean syringes from sponsors too. Buy X get one free, another free one for being a member and then a free 'gift'. Half ended contam or bunk and a huge waste of time, effort and materials. Agar only for now on!  Good luck, you're not alone!


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Invisibleskuzzy1232
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: tahoe]
    #22156688 - 08/28/15 01:40 PM (8 years, 5 months ago)

Quote:

tahoe said:
What size bags or jars did you pc for 90 mins? How many cups of seed were in each one?
When I use a spore syringe or lc I will not shake the bag until I see growth.



Quote:

tahoe said:


Each bag were large spawn bags with five quarts of seed per bag.


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .


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Invisibletahoe
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: skuzzy1232]
    #22159251 - 08/29/15 03:29 AM (8 years, 5 months ago)

Well there you go. 90 mins for a large bag with 5 quarts. Get a few of those test tube thingy's and stick one dead center of a bag. Pc it for 90 mins and then check to see if the center of the bag is sterile. I bet not. I think rr goes 90 mins on his quart jars. I go 75 mins on my 1/2 gallon but they are only four inches thick.
Those large bags have to be at least 6 inches thick.
Have you had luck cooking the same sized bags for the same amount of time?


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinehamloaf
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Re: Contamination problem help, 25 bags 20 contaminated me or the sponsor ????? [Re: skuzzy1232]
    #22159257 - 08/29/15 03:40 AM (8 years, 5 months ago)

Your bags did not become sterilized properly.  90 minutes at 15 psi is not enough time in the sterilizer for 5 quart bags.  Double your sterilization time to 3 hours.

Also just prior to loading and turning on your PC you will need to let the sterilizer run with the weight regulator off or the vent cock open until steam is shooting out of the cock or weight regulator for about 10 minutes so you know the sterilization vessel and substrate all the way into it's core all rise to the same temperature at the same time.  Once steam has been escaping from the aforementioned amount of time put the weight back on or close the stopcock and allow pressure to build.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Edited by hamloaf (08/29/15 03:59 AM)


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