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OfflineCloudbase Jim
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Registered: 08/07/15
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Multi spore first?
    #22152162 - 08/27/15 03:49 PM (8 years, 5 months ago)

Hello again,

Another Noob question here, more than likely.

Would it be best to inoculate grain with a syringe first to find a specific mushroom with genes that are well suited to my particular environment and then clone that or just go straight to agar and select a favorable mycelium there?

Thanks


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: Multi spore first? [Re: Cloudbase Jim]
    #22152203 - 08/27/15 03:58 PM (8 years, 5 months ago)

To narrow down genetics and bypass contams, go with agar first! Shooting MS solution into grain jars is never a good idea because syringes are not 100% sterile.


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Invisiblegizmo1
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Registered: 06/15/11
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Re: Multi spore first? [Re: Psilosoulful]
    #22152228 - 08/27/15 04:03 PM (8 years, 5 months ago)

Yes it would be best in my opinion to put it to agar just to make sure you have a clean culture to start with. Don't need to do a bunch of transfers or anything just make sure you have clean growth.
Then take it to grains and you can clone from the fruits of your choice.


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: Multi spore first? [Re: gizmo1]
    #22152238 - 08/27/15 04:05 PM (8 years, 5 months ago)

:whathesaid:


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