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Offlineuninc4life2010
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Excessive condensation on my petri dishes?
    #22152072 - 08/27/15 03:30 PM (8 years, 5 months ago)

After pouring my petri dishes in my glovebox I noticed there was quite a lot of condensation on the inside.  How much of a problem is this?  Can the condensation lead to contamination?


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Re: Excessive condensation on my petri dishes? [Re: uninc4life2010]
    #22152126 - 08/27/15 03:42 PM (8 years, 5 months ago)

Im not sure of it can cause contamination but you can always store them upside down so the condensation doesn't get into the agar how long did you wait for the agar to cool before pouring?


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Offlineuninc4life2010
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Re: Excessive condensation on my petri dishes? [Re: gizmo1]
    #22152178 - 08/27/15 03:51 PM (8 years, 5 months ago)

Quote:

gizmo1 said:
Im not sure of it can cause contamination but you can always store them upside down so the condensation doesn't get into the agar how long did you wait for the agar to cool before pouring?





I waited about 45 minutes or so, but the bottle was definitely pretty hot when I poured the plates.


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Re: Excessive condensation on my petri dishes? [Re: uninc4life2010]
    #22152199 - 08/27/15 03:57 PM (8 years, 5 months ago)

Next time you pour petri dishes wait at least 2 hours for it to cool before pouring if not it will cause excessive condensation on the dishes.


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Re: Excessive condensation on my petri dishes? [Re: gizmo1]
    #22152211 - 08/27/15 04:00 PM (8 years, 5 months ago)

Of course I'm no pro I'm just taking this from RRs mushroom vids.
I always wait as long as I feel comfortable letting it cool. Don't want to wait too long because it will start to solidify but too soon and you will get a bunch of condensation.


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InvisiblebodhisattaMDiscordReddit
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Re: Excessive condensation on my petri dishes? [Re: gizmo1]
    #22152512 - 08/27/15 04:59 PM (8 years, 5 months ago)

here's something to read about agar

pour your agar as cool as you can before it starts to gel. 47C is a good temperature to pour at
you can cool your agar down in a water bath or if you have an good spooky magic at a distance infrared thermometer you can wait and check that way.

http://www.shroomery.org/forums/showflat.php/Number/20947447

and stropharis' agar thread

there's more out there too, frank's cloning is a good one, pasty white's easy agar tek. look around

Quote:

gizmo1 said:
Next time you pour petri dishes wait at least 2 hours for it to cool before pouring if not it will cause excessive condensation on the dishes.




it might work but that really depends on the vessel the agar is in, and the volume of media in the vessel.


Edited by Trusted cuItivator (08/27/15 05:00 PM)


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Offlineuninc4life2010
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Re: Excessive condensation on my petri dishes? [Re: bodhisatta]
    #22152712 - 08/27/15 05:36 PM (8 years, 5 months ago)

Yeah, I definitely poured the plates at too high of a temp.  Hopefully they won't be problematic down the road.  Does anyone know if the plastic plates are reusable?


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Re: Excessive condensation on my petri dishes? [Re: uninc4life2010]
    #22152852 - 08/27/15 05:58 PM (8 years, 5 months ago)

they're not if they're the ones that come in a sleeve and they're polystyrene


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Re: Excessive condensation on my petri dishes? [Re: bodhisatta]
    #22152996 - 08/27/15 06:24 PM (8 years, 5 months ago)

Quote:

bodhisatta said:
here's something to read about agar

pour your agar as cool as you can before it starts to gel. 47C is a good temperature to pour at
you can cool your agar down in a water bath or if you have an good spooky magic at a distance infrared thermometer you can wait and check that way.

http://www.shroomery.org/forums/showflat.php/Number/20947447

and stropharis' agar thread

there's more out there too, frank's cloning is a good one, pasty white's easy agar tek. look around

Quote:

gizmo1 said:
Next time you pour petri dishes wait at least 2 hours for it to cool before pouring if not it will cause excessive condensation on the dishes.




it might work but that really depends on the vessel the agar is in, and the volume of media in the vessel.



Ah yes your right I didn't consider this lol. I was going off how RR says not to disturb it in the PC for at least 2 hours due to it needing the time to cool and risk of boil over. I leave mine in a hot PC for 2 hours then take it out and transfer to SAB to chill out for awhile and continue cooling. I check it by feeling the glass bottle and seeing how cool it is. Once I feel it's ready then I pour. Cooling in water would be so much easier.


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OfflineNature Boy
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Re: Excessive condensation on my petri dishes? [Re: uninc4life2010]
    #22153116 - 08/27/15 06:46 PM (8 years, 5 months ago)

Quote:

uninc4life2010 said:
After pouring my petri dishes in my glovebox I noticed there was quite a lot of condensation on the inside.  How much of a problem is this?  Can the condensation lead to contamination?




Don't feel bad.  It's a common occurrence.  It's annoying because it obscures your view of the mycelium...but in my experience unless the lid id loaded with water to the point of raining down on the agar, the incidence of contamination isn't dramatically increased.

I've waited until there is a skim of solidifying agar inside the vessel I'm pouring from and have STILL gotten condensation in my glass petri dishes.  I'm pouring in front of a flowhood and even if I wait a minute or so holding the lid off (hate doing that) in the hopes of having the agar cool down enough to eliminate condensation, I still get some:shrug:

N.B.


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Re: Excessive condensation on my petri dishes? [Re: Nature Boy]
    #22153192 - 08/27/15 07:01 PM (8 years, 5 months ago)

Stack the petri dishes and put a warm cup of coffee on the top and it draws the condensation away

Don't turn petri dishes upside down.  The moisture will collect on the lid, and then flood the mycelium when you turn it right side up.  In addition, moisture will fall out of the agar by gravity, compounding the problem.


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Re: Excessive condensation on my petri dishes? [Re: cronicr]
    #22153720 - 08/27/15 08:41 PM (8 years, 5 months ago)

Quote:

cronicr said:
Stack the petri dishes and put a warm cup of coffee on the top and it draws the condensation away

Don't turn petri dishes upside down.  The moisture will collect on the lid, and then flood the mycelium when you turn it right side up.  In addition, moisture will fall out of the agar by gravity, compounding the problem.



Corrected once again I need to add a disclaimer to my sig that I constantly give outdated misinformation. Lol. I haven't grown for a while and I've been on a long break from shroomery. Thanks for that tip BTW cron I gotta try that.


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Re: Excessive condensation on my petri dishes? [Re: gizmo1]
    #22153723 - 08/27/15 08:43 PM (8 years, 5 months ago)

:super: no problem


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Re: Excessive condensation on my petri dishes? [Re: uninc4life2010]
    #22164755 - 08/30/15 01:01 PM (8 years, 4 months ago)

Quote:

uninc4life2010 said:
Does anyone know if the plastic plates are reusable?



Standard polystyrene ones will deform if heated. Some will reuse them using peroxidated agar, after washing the plates well and rinsing in peroxidated water. This might be good enough for doing transfers to, and eventually put them on normal plates if its to be long term. Some would say its not worth the risk and/or effort.

I have some peroxidated agar I made months ago which is still clean.


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Re: Excessive condensation on my petri dishes? [Re: blackout]
    #22165065 - 08/30/15 02:41 PM (8 years, 4 months ago)

Sterilization itself degrades all the peroxide. Brown bottle peroxide a disinfectant not a sanitizer.


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Offlineblackout
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Re: Excessive condensation on my petri dishes? [Re: bodhisatta]
    #22165897 - 08/30/15 06:36 PM (8 years, 4 months ago)

Quote:

bodhisatta said:
Sterilization itself degrades all the peroxide. Brown bottle peroxide a disinfectant not a sanitizer.


Not sure what you are getting at. You heat treat the agar as normal, and when its cooling and almost at pouring temp you add the peroxide. Then pour it into your plates.


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OfflineMachiavelliavore
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Re: Excessive condensation on my petri dishes? [Re: blackout]
    #22166081 - 08/30/15 07:22 PM (8 years, 4 months ago)

I get condensation on my no pours.  I just give'm a solid tap whack before use, then pour off the water in the airbox.

I think the main problem with water is that it can spread a contam all over when you move the plate.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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