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InvisibleTiN 42
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Registered: 12/15/11
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Petri help please
    #22151042 - 08/27/15 11:36 AM (8 years, 5 months ago)

Can anyone explain what happened here. This culture was nice and rhizomorphic. Now this is it contamination?



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InvisibleGrey
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Re: Petri help please [Re: TiN 42]
    #22151058 - 08/27/15 11:38 AM (8 years, 5 months ago)

Did you change up your agar recipe/prep?


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InvisibleTiN 42
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Re: Petri help please [Re: Grey]
    #22151085 - 08/27/15 11:44 AM (8 years, 5 months ago)

I don't have any pics of the first dish but it was at least six months old and I think it might have been PDA. I have changed to MEA since then.


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InvisibleStuey1
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Re: Petri help please [Re: Grey]
    #22151102 - 08/27/15 11:47 AM (8 years, 5 months ago)

Try making up a lesser nutrient agar mix for your next plate


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InvisibleTiN 42
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Re: Petri help please [Re: Stuey1]
    #22151149 - 08/27/15 11:59 AM (8 years, 5 months ago)

So can I just put less light malt extract? Right now the recipe I use is: 4.5 grams agar, 5 grams light malt extract, and 250ml water.


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InvisibleStuey1
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Re: Petri help please [Re: TiN 42]
    #22151372 - 08/27/15 12:57 PM (8 years, 5 months ago)

Yes. The agar itself isn't nutritious. Its just a solidifying agent and mycelium won't grow on that. The malt extract is what gives it nutrients. So tone it down a little bit. If your using 5 grams is try 4.5-4.7 grams and go from there. I use the pre made stuff so I just dilute my mix if needed.

Think of it like this if you have a nutrient rich plate than the mycelium doesn't need to work as hard to get food. But when you use less nutrients your mycelium will have to work harder to get what it needs and will grow in a more aggressive manner to reach for that food supply.

You also have to take in co sideration that plate has sat dormant for 6 months and this was your first transfer. It could work itself out. But if not try lessening the nutrients.

Another thing I feel people get to wrapped up in the "it has to be rhino growth or it went perform well in the end". Yes that is what we like to see but I have got damn good hauls off lesser rhino looking mycelium plenty of times.

And yet another thing. I don't let my plates fill up quite that much. Once the mycelium has grown out from your transfer about a half inch transfer again. The more you let it expand the higher risk you run of it running into something that could have fallen on your plate while you were doing your last transfer.

Good luck bud


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Edited by Stuey1 (08/27/15 01:07 PM)


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InvisibleTiN 42
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Re: Petri help please [Re: Stuey1]
    #22151409 - 08/27/15 01:04 PM (8 years, 5 months ago)

Thanks again I have read most of that but have never experienced it. :thanx::cheers:


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