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Offlinecheesie
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voyage of discovery
    #22150303 - 08/27/15 08:29 AM (8 years, 5 months ago)

So a little duckling waddled in one day with his new purchases...

One shiny new pressure cooker - check

Four packets of agar agar from the Chinese supply shop - check

Black food colouring - check

Six 250ml pp5 circular plastic containers with lids - check

Pack of instant potato - check

Golden syrup - check

And some prints - check


And the journey continues......


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Invisiblewowimflabbergasted
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Re: voyage of discovery [Re: cheesie]
    #22150339 - 08/27/15 08:37 AM (8 years, 5 months ago)

:ducklol:


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OfflinetaGyo
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22150376 - 08/27/15 08:49 AM (8 years, 5 months ago)

:slowreaction:


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Offlinecheesie
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Re: voyage of discovery [Re: taGyo]
    #22150736 - 08/27/15 10:17 AM (8 years, 5 months ago)

The duckling is Planning to make a start after I have prepped everything. How long do you put the agar into the PC for if the PC is at 10 psi?


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Offlinecheesie
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Re: voyage of discovery [Re: cheesie]
    #22154819 - 08/28/15 03:06 AM (8 years, 5 months ago)

Today the duckling is PCing his agar ....



Fingers crossed.


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Offlinecheesie
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Re: voyage of discovery [Re: cheesie]
    #22154857 - 08/28/15 03:42 AM (8 years, 5 months ago)

Three done three inhttp://


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Invisiblewowimflabbergasted
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Re: voyage of discovery [Re: cheesie]
    #22155143 - 08/28/15 07:06 AM (8 years, 5 months ago)

Stop saying you're a little duckling. It's fuckin weird.


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Offlinecheesie
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22155380 - 08/28/15 08:43 AM (8 years, 5 months ago)

Hehe the duckling has now inoculated 6 using a SAB and fingers crossed now.


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Invisiblewowimflabbergasted
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Re: voyage of discovery [Re: cheesie]
    #22155472 - 08/28/15 09:10 AM (8 years, 5 months ago)

Ducks have webbed feet.....and no fingers....only feet. If they did have fingers though they would still be webbed and unable to be crossed. Hmmm....


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Offlinecheesie
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22155587 - 08/28/15 09:38 AM (8 years, 5 months ago)

Ducklings use their beaks a lot to achieve their tasks


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Re: voyage of discovery [Re: cheesie]
    #22155628 - 08/28/15 09:49 AM (8 years, 5 months ago)

You must be the smartest duck ever.


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OfflinetaGyo
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22155634 - 08/28/15 09:51 AM (8 years, 5 months ago)

Quote:

wowimflabbergasted said:
You must be the smartest duck ever.



:laugh2::laugh2:


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Offlinecheesie
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Re: voyage of discovery [Re: taGyo]
    #22155666 - 08/28/15 09:58 AM (8 years, 5 months ago)

Ducks are smart always watching always listening always trying... just need a bit of bread thrown at us once in a while


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Re: voyage of discovery [Re: cheesie]
    #22155685 - 08/28/15 10:01 AM (8 years, 5 months ago)

Enough with the duck stuff, we know it's you :lol:


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Re: voyage of discovery [Re: cheesie]
    #22155687 - 08/28/15 10:01 AM (8 years, 5 months ago)

Quack


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Offlinecheesie
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22155725 - 08/28/15 10:09 AM (8 years, 5 months ago)

Duck waddles around flapping in excitement . How long do I wait for the shizzle to grow


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Re: voyage of discovery [Re: cheesie]
    #22155747 - 08/28/15 10:14 AM (8 years, 5 months ago)

As long as it takes for you to realize you're not a duck :facepalm:

You should see growth in the next 3-14 days. 3 being quick, 7 being average, 14 being very long.


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Re: voyage of discovery [Re: taGyo]
    #22155809 - 08/28/15 10:24 AM (8 years, 5 months ago)

Is that duck time or human time?


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OfflinetaGyo
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22155817 - 08/28/15 10:25 AM (8 years, 5 months ago)



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Re: voyage of discovery [Re: taGyo]
    #22155831 - 08/28/15 10:27 AM (8 years, 5 months ago)

knock the shit off or crons going duck hunting:lol:


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Re: voyage of discovery [Re: cronicr]
    #22155841 - 08/28/15 10:28 AM (8 years, 5 months ago)

:laugh2:

Oh man, that's like the third thread I've started laughing out loud about. Missed this place these last two days, real life caught up to me :sad:


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Offlinecheesie
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Re: voyage of discovery [Re: cronicr]
    #22155944 - 08/28/15 10:48 AM (8 years, 5 months ago)

Quote:

cronicr said:
knock the shit off or crons going duck hunting:lol:




Duck quivers with fear please don't kill any ducks


.... no ducks were harmed during this thread.


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Offlinecheesie
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22155952 - 08/28/15 10:50 AM (8 years, 5 months ago)

Quote:

wowimflabbergasted said:
Is that duck time or human time?



Human time or I'd be about 80 before it grows


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OfflinecronicrFacebook
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Re: voyage of discovery [Re: cheesie]
    #22157213 - 08/28/15 03:33 PM (8 years, 5 months ago)

:firstladyofapproval:


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Offlinecheesie
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Re: voyage of discovery [Re: cronicr]
    #22157333 - 08/28/15 04:04 PM (8 years, 5 months ago)

:adventuretime:  :patlal:


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Re: voyage of discovery [Re: cheesie]
    #22167612 - 08/31/15 03:45 AM (8 years, 4 months ago)

Duckling has Taken a quick look this morning and noticed a small mycelium growth... exciting times. Will get mummy duck to try and get a pic .. quack quack


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Invisiblewowimflabbergasted
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Re: voyage of discovery [Re: cheesie]
    #22168328 - 08/31/15 10:05 AM (8 years, 4 months ago)

You're retarded.


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OfflineYerow
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22168357 - 08/31/15 10:15 AM (8 years, 4 months ago)

I love this guy, stop being mean :frown:


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Re: voyage of discovery [Re: Yerow]
    #22168380 - 08/31/15 10:20 AM (8 years, 4 months ago)



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Offlinecheesie
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22168382 - 08/31/15 10:21 AM (8 years, 4 months ago)

Pics from the duck household



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Re: voyage of discovery [Re: cheesie]
    #22168385 - 08/31/15 10:22 AM (8 years, 4 months ago)

It is alive!! :rockon:


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Offlinecheesie
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Re: voyage of discovery [Re: cheesie]
    #22168391 - 08/31/15 10:23 AM (8 years, 4 months ago)

Little fluffy duckling is excited. Flapping around (away from the sterile areas) and doing the duck dance  :cookiemonster:  :duckie:


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Offlinecheesie
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Re: voyage of discovery [Re: cheesie]
    #22168405 - 08/31/15 10:26 AM (8 years, 4 months ago)

Quack quack ... duckling can see little white fluffy spots of mycelium popping up like daisies. .. duck likes daisy's


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Re: voyage of discovery [Re: cheesie]
    #22168407 - 08/31/15 10:26 AM (8 years, 4 months ago)

Lol man....you ever have roasted duck? Good stuff.


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Offlinecheesie
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22168411 - 08/31/15 10:27 AM (8 years, 4 months ago)

This duckling has teeth ya know


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OfflinetaGyo
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22168413 - 08/31/15 10:27 AM (8 years, 4 months ago)

Quote:

wowimflabbergasted said:




:laugh2::laugh2::laugh2:,

Dude seriously cut the duck shit out. If the cops wanted you, they'd have you for logging into the shroomery.


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Re: voyage of discovery [Re: cheesie]
    #22168421 - 08/31/15 10:28 AM (8 years, 4 months ago)

So glad duckling made the agar black. Really see the growth so easily


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Re: voyage of discovery [Re: taGyo]
    #22168423 - 08/31/15 10:29 AM (8 years, 4 months ago)

I just think he's 14 tag :shrug:


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Offlinecheesie
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Re: voyage of discovery [Re: wowimflabbergasted]
    #22168432 - 08/31/15 10:31 AM (8 years, 4 months ago)

Quote:

wowimflabbergasted said:
I just think he's 14 tag :shrug:





Haha duckling chuckles


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Offlinetripdawg420
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Re: voyage of discovery [Re: cheesie]
    #22168449 - 08/31/15 10:35 AM (8 years, 4 months ago)

:laugh2:


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Re: voyage of discovery [Re: wowimflabbergasted]
    #22168458 - 08/31/15 10:36 AM (8 years, 4 months ago)

Quote:

wowimflabbergasted said:
I just think he's 14 tag :shrug:



How do you help these people :facepalm:


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Re: voyage of discovery [Re: taGyo]
    #22168466 - 08/31/15 10:39 AM (8 years, 4 months ago)

I'm not :lol:


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Re: voyage of discovery [Re: wowimflabbergasted]
    #22168482 - 08/31/15 10:43 AM (8 years, 4 months ago)

is duck liver anything like goose liver?


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Re: voyage of discovery [Re: spacechildo]
    #22168520 - 08/31/15 10:52 AM (8 years, 4 months ago)

Quote:

spacechildo said:
is duck liver anything like goose liver?




Erm yes.. I think


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Re: voyage of discovery [Re: cheesie]
    #22168528 - 08/31/15 10:54 AM (8 years, 4 months ago)

Duckling registered 2006 so duck was a great age of 8 ... hehe


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Re: voyage of discovery [Re: cheesie]
    #22168530 - 08/31/15 10:54 AM (8 years, 4 months ago)

Or less


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Re: voyage of discovery [Re: cheesie]
    #22168538 - 08/31/15 10:56 AM (8 years, 4 months ago)

Well enjoy being a duck, I'm gonna hide this thread now :laugh2:

Good for a great laugh, not a serious grow though.


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Re: voyage of discovery [Re: cheesie]
    #22168542 - 08/31/15 10:57 AM (8 years, 4 months ago)

Did one notice the new pics?



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Re: voyage of discovery [Re: taGyo]
    #22168552 - 08/31/15 10:59 AM (8 years, 4 months ago)

Quote:

taGyo said:
Well enjoy being a duck, I'm gonna hide this thread now :laugh2:

Good for a great laugh, not a serious grow though.



:whathesaid: cya


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Re: voyage of discovery [Re: wowimflabbergasted]
    #22168670 - 08/31/15 11:25 AM (8 years, 4 months ago)

Thread vote... duck talk or no duck talk?


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Re: voyage of discovery [Re: cheesie]
    #22168678 - 08/31/15 11:27 AM (8 years, 4 months ago)

aww, I guess if you want more replies, no duck talk :sad:


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Re: voyage of discovery [Re: Yerow]
    #22170380 - 08/31/15 06:06 PM (8 years, 4 months ago)

:whathesaid:


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Re: voyage of discovery [Re: taGyo]
    #22172366 - 09/01/15 02:38 AM (8 years, 4 months ago)

Three of the dishes are now showing mycelium growth (no duck talk)



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Re: voyage of discovery [Re: cheesie]
    #22172379 - 09/01/15 02:46 AM (8 years, 4 months ago)

Looks good! Few days from your first transfer it seems :thumbup:


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Re: voyage of discovery [Re: Yerow]
    #22172383 - 09/01/15 02:48 AM (8 years, 4 months ago)

Quote:

Yerow said:
Looks good! Few days from your first transfer it seems :thumbup:




Yeah only a few days but exciting. Wondering what warning signs to watch for ie contamination


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Re: voyage of discovery [Re: cheesie]
    #22172879 - 09/01/15 08:07 AM (8 years, 4 months ago)

:rockon:


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Re: voyage of discovery [Re: taGyo]
    #22172944 - 09/01/15 08:26 AM (8 years, 4 months ago)

Here is a pretty good link to what the good and bad stuff looks like.

How it should & shouldn't look


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Re: voyage of discovery [Re: Yerow]
    #22172966 - 09/01/15 08:32 AM (8 years, 4 months ago)

Tmethyl is a fucking genius, don't forget to rate him.


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Re: voyage of discovery [Re: taGyo]
    #22176787 - 09/02/15 01:52 AM (8 years, 4 months ago)

Update ... worried some look like contamination. Can you help I'd if any are contamination?



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Re: voyage of discovery [Re: cheesie]
    #22176821 - 09/02/15 02:11 AM (8 years, 4 months ago)

The large spots on picture 2,3 are not cube mycelium sadly. Might have a shot with the first plate


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Re: voyage of discovery [Re: Yerow]
    #22176832 - 09/02/15 02:22 AM (8 years, 4 months ago)

Quote:

Yerow said:
The large spots on picture 2,3 are not cube mycelium sadly. Might have a shot with the first plate




Sad times. Hoping the first works ok. Otherwise back to the drawing board and a restart. Need to recheck my sterile techniques if the first one fails


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Re: voyage of discovery [Re: cheesie]
    #22176957 - 09/02/15 03:56 AM (8 years, 4 months ago)

You can let the other 2 grow out some more and try to transfer away from the contam.  Might have a chance if the cube mycelium grows a bit, seen some seriously nasty plates that got cleaned nicely up.

Try to snap a few more pics and also, check out  Stro's Cleaning and Isolating On Agar :rockon:


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Re: voyage of discovery [Re: Yerow]
    #22176981 - 09/02/15 04:15 AM (8 years, 4 months ago)

Quote:

Yerow said:
You can let the other 2 grow out some more and try to transfer away from the contam.  Might have a chance if the cube mycelium grows a bit, seen some seriously nasty plates that got cleaned nicely up.

Try to snap a few more pics and also, check out  Stro's Cleaning and Isolating On Agar :rockon:






When I'm taking pics what do I need to capture?


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Re: voyage of discovery [Re: cheesie]
    #22177024 - 09/02/15 04:46 AM (8 years, 4 months ago)

As much of the surface and as close without it blurring up as you can. I dislike no pour plates for this reason. Petris are much easier to see through :tongue2:


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Re: voyage of discovery [Re: Yerow]
    #22177061 - 09/02/15 05:15 AM (8 years, 4 months ago)

I had a look at the link but notice there seems to be two colours going on on the separate growths ones like a bluey green (bad) the others like a light tan colour. Is the tan colour normal? These are Mexicana


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Re: voyage of discovery [Re: cheesie]
    #22177080 - 09/02/15 05:27 AM (8 years, 4 months ago)

No the ones with those colors are bad :thumbdown:


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Re: voyage of discovery [Re: Yerow]
    #22177092 - 09/02/15 05:41 AM (8 years, 4 months ago)

Oh .... hmmm need more pics I think


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Re: voyage of discovery [Re: cheesie]
    #22177352 - 09/02/15 08:27 AM (8 years, 4 months ago)

Plate #2



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Re: voyage of discovery [Re: cheesie]
    #22177378 - 09/02/15 08:40 AM (8 years, 4 months ago)

Plate #3



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Re: voyage of discovery [Re: cheesie]
    #22177380 - 09/02/15 08:41 AM (8 years, 4 months ago)

Can't get a good pic of plate 1 but it doesn't seem as flat as the others more fluffy.


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Re: voyage of discovery [Re: cheesie]
    #22177385 - 09/02/15 08:47 AM (8 years, 4 months ago)

nothing good there :sad:


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Re: voyage of discovery [Re: Yerow]
    #22177397 - 09/02/15 08:55 AM (8 years, 4 months ago)

Also plate 1 is a contam. I know others say it's too early to tell, but there's already a green discoloration in the first pics.


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Re: voyage of discovery [Re: Mad Season]
    #22177415 - 09/02/15 09:04 AM (8 years, 4 months ago)

Do i bin them and restart?


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Re: voyage of discovery [Re: cheesie]
    #22177431 - 09/02/15 09:10 AM (8 years, 4 months ago)

I wouldn't toss them for a few days. I actually prefer green mold contams on agar. They don't spread much after sporulation. Unlike some bacterias that take the whole plate over in a day..

I'd just wait for cube myc to germinate in other spots far away from the green. When it gets just barely big enough to cut out, transfer ASAP and let it grow on a fresh clean plate.


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Edited by Mad Season (09/02/15 09:11 AM)


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Re: voyage of discovery [Re: Mad Season]
    #22177500 - 09/02/15 09:49 AM (8 years, 4 months ago)

Iv just done 14 fresh plates for possible transfers.

Still a little confused about what I need to be seeing.


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Re: voyage of discovery [Re: cheesie]
    #22177506 - 09/02/15 09:51 AM (8 years, 4 months ago)



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Re: voyage of discovery [Re: Mad Season]
    #22177519 - 09/02/15 09:56 AM (8 years, 4 months ago)

Yeah they look very clean in comparison to mine


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Re: voyage of discovery [Re: cheesie]
    #22177578 - 09/02/15 10:20 AM (8 years, 4 months ago)

And I've had plates with more green than yours i still got clean germination and a transfer out of. I couldn't take a pic because they were pasty plates and not petris. But you're looking for mycelium close looking to those :smile:. Give it some more time. Lots of times green germinates first. Not sure why :shrug:


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Re: voyage of discovery [Re: Mad Season]
    #22177724 - 09/02/15 11:05 AM (8 years, 4 months ago)

Cheers for the advice and support. Will post more pics as things change


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Re: voyage of discovery [Re: cheesie]
    #22178733 - 09/02/15 03:45 PM (8 years, 4 months ago)

Number 2 is a contam.

Number 3 looks weird, I'd let that grow a bit or open it in an SAB and get a better look. I've had number 2, that's aspergillus.


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Re: voyage of discovery [Re: cheesie]
    #22187586 - 09/04/15 11:31 AM (8 years, 4 months ago)

So all three plates are contaminated with no signs of the print spores growing.iv got 14 new no pour plates done and will have to try again.


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