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cheesie
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Registered: 07/03/06
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Re: voyage of discovery [Re: Yerow]
#22176832 - 09/02/15 02:22 AM (8 years, 4 months ago) |
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Quote:
Yerow said: The large spots on picture 2,3 are not cube mycelium sadly. Might have a shot with the first plate
Sad times. Hoping the first works ok. Otherwise back to the drawing board and a restart. Need to recheck my sterile techniques if the first one fails
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Yerow
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Re: voyage of discovery [Re: cheesie]
#22176957 - 09/02/15 03:56 AM (8 years, 4 months ago) |
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You can let the other 2 grow out some more and try to transfer away from the contam. Might have a chance if the cube mycelium grows a bit, seen some seriously nasty plates that got cleaned nicely up.
Try to snap a few more pics and also, check out Stro's Cleaning and Isolating On Agar
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cheesie
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Re: voyage of discovery [Re: Yerow]
#22176981 - 09/02/15 04:15 AM (8 years, 4 months ago) |
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Quote:
Yerow said: You can let the other 2 grow out some more and try to transfer away from the contam. Might have a chance if the cube mycelium grows a bit, seen some seriously nasty plates that got cleaned nicely up.
Try to snap a few more pics and also, check out Stro's Cleaning and Isolating On Agar 
When I'm taking pics what do I need to capture?
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Yerow
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Re: voyage of discovery [Re: cheesie]
#22177024 - 09/02/15 04:46 AM (8 years, 4 months ago) |
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As much of the surface and as close without it blurring up as you can. I dislike no pour plates for this reason. Petris are much easier to see through
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cheesie
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Re: voyage of discovery [Re: Yerow]
#22177061 - 09/02/15 05:15 AM (8 years, 4 months ago) |
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I had a look at the link but notice there seems to be two colours going on on the separate growths ones like a bluey green (bad) the others like a light tan colour. Is the tan colour normal? These are Mexicana
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Yerow
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Re: voyage of discovery [Re: cheesie]
#22177080 - 09/02/15 05:27 AM (8 years, 4 months ago) |
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No the ones with those colors are bad
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cheesie
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Re: voyage of discovery [Re: Yerow]
#22177092 - 09/02/15 05:41 AM (8 years, 4 months ago) |
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Oh .... hmmm need more pics I think
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cheesie
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Re: voyage of discovery [Re: cheesie]
#22177352 - 09/02/15 08:27 AM (8 years, 4 months ago) |
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Plate #2
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cheesie
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Re: voyage of discovery [Re: cheesie]
#22177378 - 09/02/15 08:40 AM (8 years, 4 months ago) |
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Plate #3
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cheesie
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Re: voyage of discovery [Re: cheesie]
#22177380 - 09/02/15 08:41 AM (8 years, 4 months ago) |
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Can't get a good pic of plate 1 but it doesn't seem as flat as the others more fluffy.
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Yerow
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Re: voyage of discovery [Re: cheesie]
#22177385 - 09/02/15 08:47 AM (8 years, 4 months ago) |
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nothing good there
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Mad Season
hookers and blackjack



Registered: 09/16/12
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Re: voyage of discovery [Re: Yerow]
#22177397 - 09/02/15 08:55 AM (8 years, 4 months ago) |
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Also plate 1 is a contam. I know others say it's too early to tell, but there's already a green discoloration in the first pics.
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cheesie
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Do i bin them and restart?
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Mad Season
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Registered: 09/16/12
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Re: voyage of discovery [Re: cheesie]
#22177431 - 09/02/15 09:10 AM (8 years, 4 months ago) |
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I wouldn't toss them for a few days. I actually prefer green mold contams on agar. They don't spread much after sporulation. Unlike some bacterias that take the whole plate over in a day..
I'd just wait for cube myc to germinate in other spots far away from the green. When it gets just barely big enough to cut out, transfer ASAP and let it grow on a fresh clean plate.
Edited by Mad Season (09/02/15 09:11 AM)
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cheesie
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Iv just done 14 fresh plates for possible transfers.
Still a little confused about what I need to be seeing.
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Mad Season
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Registered: 09/16/12
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Re: voyage of discovery [Re: cheesie]
#22177506 - 09/02/15 09:51 AM (8 years, 4 months ago) |
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cheesie
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Registered: 07/03/06
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Yeah they look very clean in comparison to mine
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Mad Season
hookers and blackjack



Registered: 09/16/12
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Re: voyage of discovery [Re: cheesie]
#22177578 - 09/02/15 10:20 AM (8 years, 4 months ago) |
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And I've had plates with more green than yours i still got clean germination and a transfer out of. I couldn't take a pic because they were pasty plates and not petris. But you're looking for mycelium close looking to those . Give it some more time. Lots of times green germinates first. Not sure why
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cheesie
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Cheers for the advice and support. Will post more pics as things change
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taGyo
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Re: voyage of discovery [Re: cheesie]
#22178733 - 09/02/15 03:45 PM (8 years, 4 months ago) |
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Number 2 is a contam.
Number 3 looks weird, I'd let that grow a bit or open it in an SAB and get a better look. I've had number 2, that's aspergillus.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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