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Offlinetopsykretts
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Spore syringes, or spore solution?
    #22146182 - 08/26/15 12:29 PM (8 years, 5 months ago)

I just made a SAB so I can finally use my spore print to make some new syringes.  I was thinking though, instead of making individual syringes and re-exposing the print to contamination each time, I could just scrape all of the spores into a sterilized jar of water with an 'injection port' and then suck the spore solution into sterilized syringes when I need to make new syringes.  Is there a problem with this idea?


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Re: Spore syringes, or spore solution? [Re: topsykretts]
    #22146257 - 08/26/15 12:40 PM (8 years, 5 months ago)

nope that's fine


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Offlinetopsykretts
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Re: Spore syringes, or spore solution? [Re: topsykretts]
    #22146930 - 08/26/15 02:44 PM (8 years, 5 months ago)

Another question: should I use a lid without gas exchange filter and only an injection port?


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Re: Spore syringes, or spore solution? [Re: topsykretts]
    #22146942 - 08/26/15 02:46 PM (8 years, 5 months ago)

yep:super:


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InvisiblebodhisattaMDiscordReddit
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Re: Spore syringes, or spore solution? [Re: cronicr]
    #22146951 - 08/26/15 02:48 PM (8 years, 5 months ago)

It will make a slight vacuum so its a bit hard to fill your syringe but you definitely don't need gas exchange


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Offlinetopsykretts
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Re: Spore syringes, or spore solution? [Re: topsykretts]
    #22146973 - 08/26/15 02:51 PM (8 years, 5 months ago)

Well I mean, would the spores activate somehow or otherwise be harmed if there were a gas exchange port?  If it won't hurt to have one, then I'd put one so it is easier to fill the syringes.


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InvisiblebodhisattaMDiscordReddit
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Re: Spore syringes, or spore solution? [Re: topsykretts]
    #22146989 - 08/26/15 02:53 PM (8 years, 5 months ago)

As long as the gas exchange port is kept from getting wet(don't shake be careful) or if you use a syringe filter etc.. It does.t hurt to have the GE it won't make spores go bad or anything but it makes it slightly easier to aspirate a few syringes especially if you do them in a row


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Re: Spore syringes, or spore solution? [Re: bodhisatta]
    #22147020 - 08/26/15 02:57 PM (8 years, 5 months ago)

Quote:

bodhisatta said:
It will make a slight vacuum so its a bit hard to fill your syringe but you definitely don't need gas exchange



oh yes also this! i forget i use my lids upside down, i remember there was a site that sold lc's with just a ship and when a user went to inoculate it it sucked all 12 cc's of his syringe in:laugh2:


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InvisibleMad Season
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Re: Spore syringes, or spore solution? [Re: cronicr]
    #22147073 - 08/26/15 03:07 PM (8 years, 5 months ago)

LOL really?? That's wild! I'd be so pissed too..


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Offlineinvitro

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Re: Spore syringes, or spore solution? [Re: bodhisatta]
    #22147108 - 08/26/15 03:12 PM (8 years, 5 months ago)

Quote:

bodhisatta said:
It will make a slight vacuum so its a bit hard to fill your syringe but you definitely don't need gas exchange




I've been playing around with jars and vacuums recently...

The "slightness" of the vacuum is highly variable depending on 2 things.  1 how much air space is in the jar, 2 how old the rubber seal is on the metal lid top (assuming your using a ball style glass quart jar).  At one extreme if you have 500ml of fluid and the rest is air space, coupled with a brand new lid, the seal will be very good and the vacuum can suck in over 450ml of air before pressure equalizes.  Most people however re-use their lids and the rubber becomes harder and less able to conform the minor irregularities in the shape of the lip of the jar, so you can easily have a situation where there is no vacuum, or a slight vacuum that pulls 20 or 200 or 600 ccs.  You can give your jar/lid combo a test run and see what kind of vacuum it pulls, or maybe simpler yet, just add some GE mechanism...


Edited by invitro (08/26/15 05:13 PM)


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Offlinetopsykretts
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Re: Spore syringes, or spore solution? [Re: invitro]
    #22147139 - 08/26/15 03:19 PM (8 years, 5 months ago)

I just made the lid.  I used a small piece of EZ-felt with some post office tyvek over it for the gas port.  Had contamination issues in the past with just tyvek (may or may not have been caused by the tyvek, but that's what I'm going with) so this is my first attempt using both.  Using a salsa jar.  Hopefully I will have no issues.


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Re: Spore syringes, or spore solution? [Re: topsykretts]
    #22147247 - 08/26/15 03:39 PM (8 years, 5 months ago)

Quote:

topsykretts said:
I just made a SAB so I can finally use my spore print to make some new syringes.  I was thinking though, instead of making individual syringes and re-exposing the print to contamination each time, I could just scrape all of the spores into a sterilized jar of water with an 'injection port' and then suck the spore solution into sterilized syringes when I need to make new syringes.  Is there a problem with this idea?



Agar bro you'll never go back to syringes


--------------------
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Offlinetopsykretts
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Re: Spore syringes, or spore solution? [Re: Grim767]
    #22147261 - 08/26/15 03:42 PM (8 years, 5 months ago)

Quote:

Grim767 said:
Quote:

topsykretts said:
I just made a SAB so I can finally use my spore print to make some new syringes.  I was thinking though, instead of making individual syringes and re-exposing the print to contamination each time, I could just scrape all of the spores into a sterilized jar of water with an 'injection port' and then suck the spore solution into sterilized syringes when I need to make new syringes.  Is there a problem with this idea?



Agar bro you'll never go back to syringes




Some day.


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InvisiblePastywhyteMDiscord
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Re: Spore syringes, or spore solution? [Re: topsykretts]
    #22147511 - 08/26/15 04:33 PM (8 years, 5 months ago)

Quote:

topsykretts said:
Quote:

Grim767 said:
Quote:

topsykretts said:
I just made a SAB so I can finally use my spore print to make some new syringes.  I was thinking though, instead of making individual syringes and re-exposing the print to contamination each time, I could just scrape all of the spores into a sterilized jar of water with an 'injection port' and then suck the spore solution into sterilized syringes when I need to make new syringes.  Is there a problem with this idea?



Agar bro you'll never go back to syringes




Some day.




Tomorrow is someday :awesomenod:

Its easier than pf tek :elmo:


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Offlineblackout
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Re: Spore syringes, or spore solution? [Re: Pastywhyte]
    #22147552 - 08/26/15 04:43 PM (8 years, 5 months ago)

To help against vacuums you can sterilize your empty syringes with the plunger pulled back. Now stick it in the jar and inject air into the jar and pull out your liquid, so you are pressurizing it and then equalizing it again.

If its a brand new sterile syringe you can draw in air in a SAB, or in open air you can flame the needle and slowly draw in air which should have any contaminants killed. You can also just open the jar in a SAB if a vacuum has formed.

I used to also have 2 SHIPs on my jars, you can inject a filtered syringe needle and draw up liquid with the other. This way you do not have to have lots of filters, and if making LCs you can shake the hell out of the jar with no risk of the filter getting wet.


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Invisibletahoe
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Re: Spore syringes, or spore solution? [Re: topsykretts]
    #22147553 - 08/26/15 04:43 PM (8 years, 5 months ago)

It would not hurt to have a case of these lying around. I use them to cut or expand a new spore syringe when I get them from a vendor. Before I use the syringe on a spawn jar I inject about 1cc of spore laden water into the bottle. Now I just turned a 10-12cc 15$ spore syringe into five spore syringes.




You could do the same thing. Make a spore syringe, maybe test it. Then squirt it into your pint jar. Now you have a shit load of spore water.


Edited by tahoe (08/26/15 04:45 PM)


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Offlinetopsykretts
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Re: Spore syringes, or spore solution? [Re: tahoe]
    #22147725 - 08/26/15 05:17 PM (8 years, 5 months ago)

It is surprisingly hard to find a jar suitable for this.  I ditched the salsa jar idea when I figured out that it would be difficult to get the syringe down into the spore-water.  Went with a small jelly jar, its the same height but less volume.. still gonna have to tip it on its side.  Gonna order some of these syringes when I have the money.  http://www.shopmedvet.com/product/80545

Or maybe these.. ?  http://www.shopmedvet.com/product/ndl-spinal-26x3-1-2-each-SN26X3-5


Edited by topsykretts (08/26/15 05:20 PM)


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Offlineinvitro

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Re: Spore syringes, or spore solution? [Re: topsykretts]
    #22147770 - 08/26/15 05:26 PM (8 years, 5 months ago)

14 gauge is more than you need, you can do 18g or (presumably) less for spore water.  The larger the bore the more damage you do to the ship, and I suspect the more likely pieces of the ship will get stuck in the needle.  It's a significant problem if you intent to pierce the ship multiple times, you can tear a permanent hole in the ship.  I recently used a 16g and pierced a ship 5 different times and got a hole that would not seal so I switched to 18g.


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Invisibletahoe
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Re: Spore syringes, or spore solution? [Re: invitro]
    #22149768 - 08/27/15 02:07 AM (8 years, 5 months ago)

Topsy, I liked to use the tall 1/2 pint jars for this. Not hard to find


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