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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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I don't remember who wrote it, but I read a post a week or so ago where they put an inccense inside the SAB so they could see air movement inside the box then just made adjustments til air movement stopped. Also, keep a couple of alcohol soaked rags or paper towels on hand to wrap your needle in after flaming, it'll keep anything from landing on it until you get it back in the box.
-------------------- Bug
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Glovebox vs SAB [Re: thebug76]
#22145659 - 08/26/15 10:45 AM (8 years, 5 months ago) |
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Quote:
thebug76 said: Also, keep a couple of alcohol soaked rags or paper towels on hand to wrap your needle in after flaming, it'll keep anything from landing on it until you get it back in the box.
Not quite bud.
After flaming your needle a phenomenon called thermoporesis occurs. This repels all airborne contams because of the heat coming off of the needle. You should cool it (Pressing down the plunger) in the box 
Quote:
taGyo said: Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation. Don't wipe your needle with alcohol after flame sterilization:
Quote:
swatt_haze said: wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.
Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.
Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar. Grains is 1CC as well.
After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.
If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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Re: Glovebox vs SAB [Re: taGyo]
#22145713 - 08/26/15 10:55 AM (8 years, 5 months ago) |
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Quote:
taGyo said:
Quote:
thebug76 said: Also, keep a couple of alcohol soaked rags or paper towels on hand to wrap your needle in after flaming, it'll keep anything from landing on it until you get it back in the box.
Not quite bud.
After flaming your needle a phenomenon called thermoporesis occurs. This repels all airborne contams because of the heat coming off of the needle. You should cool it (Pressing down the plunger) in the box 
Quote:
taGyo said: Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation. Don't wipe your needle with alcohol after flame sterilization:
Quote:
swatt_haze said: wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.
Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.
Flame sterilize between each JAR, not each inoction hole. .25 cc a hole for 1cc total each BRF jar. Grains is 1CC as well.
After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.
If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.
I know mold can survive alcohol, but if you flame first, there's no mold there. The alcohol just keeps any spores from landing on the needle afterwards in case you get stalled, such as from a 3 yr old child that's super needy like mine, or any other reason.
-------------------- Bug
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Quote:
PinPornProducer said: It works 
That just about says it all.
I don't know why you would even ask about another procedure if the one you have is doing it for you. Even if you do change over, you will probably make mistakes with the new set up until you get used to it, and contamination follows mistakes like a dog follows his master--haha.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Glovebox vs SAB [Re: thebug76]
#22145739 - 08/26/15 10:59 AM (8 years, 5 months ago) |
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Yeah but alcohol takes 2 minutes to properly dry and evaporate, killing mold spores. You didn't read my thermoporesis explanation, that's the phenomenon you're after. Alcohol will only add shit to your needle.
Read that full post, it's just bad practice. If your 3 year old is running around take care of her first and then work or if she bothers you while working flame the needle again 
The alcohol as used like this is actually a vector of contam.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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Re: Glovebox vs SAB [Re: taGyo]
#22145838 - 08/26/15 11:21 AM (8 years, 5 months ago) |
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Maybe I just got lucky. I've had no contams in jars so far except a little bacteria in 2 of them and I think that was in the brf because none of the other pf jars or the wbs had any problems whatsoever. it was the same needle and I noc'd them all at the same time.
-------------------- Bug
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Glovebox vs SAB [Re: thebug76]
#22145845 - 08/26/15 11:22 AM (8 years, 5 months ago) |
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Grow for a little longer
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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Re: Glovebox vs SAB [Re: taGyo]
#22145929 - 08/26/15 11:36 AM (8 years, 5 months ago) |
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Oh yea, I know it's only a matter of time. I've only done a total of 37 jars so far, 36 of which were pf tek (brf and verm) and one wbs which I tyndallized because I don't have a pc yet. Only major problem I had was improper pasteurization of straw when trying to go to bulk with the first 24. I left the straw way too wet and only a single 1/4" hole for ge (major mistake). I threw that out because at the time I didn't know peroxide cured cobweb.
-------------------- Bug
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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Re: Glovebox vs SAB [Re: thebug76]
#22146063 - 08/26/15 12:02 PM (8 years, 5 months ago) |
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I apologize for jumping in on this thread. I burnt one and got talkative. Y'all have a good day. I'm out.
-------------------- Bug
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Glovebox vs SAB [Re: thebug76]
#22146100 - 08/26/15 12:13 PM (8 years, 5 months ago) |
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Honestly it's fine man. At least you got a good attitude and actually listen to what people say. The thing that is being said is that the flame kills all the contams. Alcohol kills 99.9% of contams. Every inch of air has 200,000 spores in it. 0.001*200,000=200 living spores. Just from an inch of air above your alcohol wipe. What's cleaner? 200 spores or 0 spores? You get 0 spores from a flame and then add 200 from the wipe. It's like taking a shower, and then wiping your face with shit right after
Edited by Mad Season (08/26/15 12:14 PM)
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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I get that. I saw the alcohol soaked paper towel thing in a video on youtube 4-5 months back when I first started reading on the subject and it stuck in my head. From what you said though, I should probably get myself out of that habit.
-------------------- Bug
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Glovebox vs SAB [Re: thebug76]
#22146280 - 08/26/15 12:42 PM (8 years, 5 months ago) |
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you won't regret it!
For the OP. A still air box is pretty much useless without being used in a room with fairly still air. By doing so you can have arm holes that are 6"+ without having to worry about drafts. Larger arm holes so when you put your arms in and out, it won't move any air. That and your skinny arms with very little excess material around them, or no material, will also move very little air around. If you do it right, and you have very little air moving around.. the only thing you have to worry about is shit hovering over open media (just your flamed tools are allowed over it). If you do so, you will see no contams.
I could imagine a well sealed glove box out performing a still air box in an open environment with lots of air movement. But if you follow the still air principle, you can have a flame going outside your box, move your arm in with no air movement around the arm holes, flame all your tools when needed, and have a Much better success rate than a glove box.
Edited by Mad Season (08/26/15 12:43 PM)
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Skinny arms? Lol
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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You know what I mean a lot less of a surface area moving than gloves.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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lol
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kushroom



Registered: 12/04/14
Posts: 588
Loc: I'm lost
Last seen: 4 months, 11 days
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Quote:
Mad Season said:
you won't regret it!
For the OP. A still air box is pretty much useless without being used in a room with fairly still air. By doing so you can have arm holes that are 6"+ without having to worry about drafts. Larger arm holes so when you put your arms in and out, it won't move any air. That and your skinny arms with very little excess material around them, or no material, will also move very little air around. If you do it right, and you have very little air moving around.. the only thing you have to worry about is shit hovering over open media (just your flamed tools are allowed over it). If you do so, you will see no contams.
I could imagine a well sealed glove box out performing a still air box in an open environment with lots of air movement. But if you follow the still air principle, you can have a flame going outside your box, move your arm in with no air movement around the arm holes, flame all your tools when needed, and have a Much better success rate than a glove box. 
Last time i tried that i blow up the inside of my SAB nd caught a paper towel soaked in iso on fire almost taking out the living room carpet lol, i think i need to switch to a flow hood cause im not to good with fire i guess, oh well all that matters is my agar is clean now
-------------------- :/
 All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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Re: Glovebox vs SAB [Re: kushroom]
#22146986 - 08/26/15 02:53 PM (8 years, 5 months ago) |
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Quote:
kushroom said:
Quote:
Mad Season said:
you won't regret it!
For the OP. A still air box is pretty much useless without being used in a room with fairly still air. By doing so you can have arm holes that are 6"+ without having to worry about drafts. Larger arm holes so when you put your arms in and out, it won't move any air. That and your skinny arms with very little excess material around them, or no material, will also move very little air around. If you do it right, and you have very little air moving around.. the only thing you have to worry about is shit hovering over open media (just your flamed tools are allowed over it). If you do so, you will see no contams.
I could imagine a well sealed glove box out performing a still air box in an open environment with lots of air movement. But if you follow the still air principle, you can have a flame going outside your box, move your arm in with no air movement around the arm holes, flame all your tools when needed, and have a Much better success rate than a glove box. 
Last time i tried that i blow up the inside of my SAB nd caught a paper towel soaked in iso on fire almost taking out the living room carpet lol, i think i need to switch to a flow hood cause im not to good with fire i guess, oh well all that matters is my agar is clean now 
Oh shit. Don't flame inside the box. Alcohol fumes are very combustible. Flame outside the SAB then put it back inside.
-------------------- Bug
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kushroom



Registered: 12/04/14
Posts: 588
Loc: I'm lost
Last seen: 4 months, 11 days
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Re: Glovebox vs SAB [Re: thebug76]
#22147021 - 08/26/15 02:57 PM (8 years, 5 months ago) |
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Haha yeah i wasnt thinking that when i did, had a couple drinks in me nd was going ham on the dabs lol
-------------------- :/
 All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Glovebox vs SAB [Re: kushroom]
#22147879 - 08/26/15 05:48 PM (8 years, 5 months ago) |
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Could just use soapy water and you wouldn't have fire.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Glovebox vs SAB [Re: Inocuole]
#22147969 - 08/26/15 06:02 PM (8 years, 5 months ago) |
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Srirachi
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