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Offlinethebug76
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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22145641 - 08/26/15 10:43 AM (8 years, 5 months ago)

I don't remember who wrote it, but I read a post a week or so ago where they put an inccense inside the SAB so they could see air movement inside the box then just made adjustments til air movement stopped. Also, keep a couple of alcohol soaked rags or paper towels on hand to wrap your needle in after flaming, it'll keep anything from landing on it until you get it back in the box.


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OfflinetaGyo
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Re: Glovebox vs SAB [Re: thebug76]
    #22145659 - 08/26/15 10:45 AM (8 years, 5 months ago)

Quote:

thebug76 said:
Also, keep a couple of alcohol soaked rags or paper towels on hand to wrap your needle in after flaming, it'll keep anything from landing on it until you get it back in the box.



Not quite bud.

After flaming your needle a phenomenon called thermoporesis occurs. This repels all airborne contams because of the heat coming off of the needle. You should cool it (Pressing down the plunger) in the box :super:

Quote:

taGyo said:
Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation.
Don't wipe your needle with alcohol after flame sterilization:

Quote:

swatt_haze said:
wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.





Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.

Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar.
Grains is 1CC as well.

After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.

If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.




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Offlinethebug76
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Re: Glovebox vs SAB [Re: taGyo]
    #22145713 - 08/26/15 10:55 AM (8 years, 5 months ago)

Quote:

taGyo said:
Quote:

thebug76 said:
Also, keep a couple of alcohol soaked rags or paper towels on hand to wrap your needle in after flaming, it'll keep anything from landing on it until you get it back in the box.



Not quite bud.

After flaming your needle a phenomenon called thermoporesis occurs. This repels all airborne contams because of the heat coming off of the needle. You should cool it (Pressing down the plunger) in the box :super:

Quote:

taGyo said:
Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation.
Don't wipe your needle with alcohol after flame sterilization:

Quote:

swatt_haze said:
wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.





Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.

Flame sterilize between each JAR, not each inoction hole. .25 cc a hole for 1cc total each BRF jar.
Grains is 1CC as well.

After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.

If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.







I know mold can survive alcohol, but if you flame first, there's no mold there. The alcohol just keeps any spores from landing on the needle afterwards in case you get stalled, such as from a 3 yr old child that's super needy like mine, or any other reason.


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Invisiblechampinhom
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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22145715 - 08/26/15 10:55 AM (8 years, 5 months ago)

Quote:

PinPornProducer said:
It works :shrug:




That just about says it all.

I don't know why you would even ask about another procedure if the one you have is doing it for you. Even if you do change over, you will probably make mistakes with the new set up until you get used to it, and contamination follows mistakes like a dog follows his master--haha.


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OfflinetaGyo
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Re: Glovebox vs SAB [Re: thebug76]
    #22145739 - 08/26/15 10:59 AM (8 years, 5 months ago)

Yeah but alcohol takes 2 minutes to properly dry and evaporate, killing mold spores. You didn't read my thermoporesis explanation, that's the phenomenon you're after. Alcohol will only add shit to your needle.

Read that full post, it's just bad practice. If your 3 year old is running around take care of her first and then work or if she bothers you while working flame the needle again :shrug:

The alcohol as used like this is actually a vector of contam.


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Offlinethebug76
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Re: Glovebox vs SAB [Re: taGyo]
    #22145838 - 08/26/15 11:21 AM (8 years, 5 months ago)

Maybe I just got lucky. I've had no contams in jars so far except a little bacteria in 2 of them and I think that was in the brf because none of the other pf jars or the wbs had any problems whatsoever. it was the same needle and I noc'd them all at the same time.


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OfflinetaGyo
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Re: Glovebox vs SAB [Re: thebug76]
    #22145845 - 08/26/15 11:22 AM (8 years, 5 months ago)

Grow for a little longer :wink:


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Offlinethebug76
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Re: Glovebox vs SAB [Re: taGyo]
    #22145929 - 08/26/15 11:36 AM (8 years, 5 months ago)

Oh yea, I know it's only a matter of time. I've only done a total of 37 jars so far, 36 of which were pf tek (brf and verm) and one wbs which I tyndallized because I don't have a pc yet. Only major problem I had was improper pasteurization of straw when trying to go to bulk with the first 24. I left the straw way too wet and only a single 1/4" hole for ge (major mistake). I threw that out because at the time I didn't know peroxide cured cobweb.


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Offlinethebug76
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Re: Glovebox vs SAB [Re: thebug76]
    #22146063 - 08/26/15 12:02 PM (8 years, 5 months ago)

I apologize for jumping in on this thread. I burnt one and got talkative. Y'all have a good day. I'm out.


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InvisibleMad Season
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Re: Glovebox vs SAB [Re: thebug76]
    #22146100 - 08/26/15 12:13 PM (8 years, 5 months ago)

Honestly it's fine man. At least you got a good attitude and actually listen to what people say. The thing that is being said is that the flame kills all the contams. Alcohol kills 99.9% of contams. Every inch of air has 200,000 spores in it. 0.001*200,000=200 living spores. Just from an inch of air above your alcohol wipe. What's cleaner? 200 spores or 0 spores? You get 0 spores from a flame and then add 200 from the wipe. It's like taking a shower, and then wiping your face with shit right after


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Edited by Mad Season (08/26/15 12:14 PM)


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Offlinethebug76
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Re: Glovebox vs SAB [Re: Mad Season]
    #22146148 - 08/26/15 12:23 PM (8 years, 5 months ago)

I get that. I saw the alcohol soaked paper towel thing in a video on youtube 4-5 months back when I first started reading on the subject and it stuck in my head. From what you said though, I should probably get myself out of that habit.


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InvisibleMad Season
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Re: Glovebox vs SAB [Re: thebug76]
    #22146280 - 08/26/15 12:42 PM (8 years, 5 months ago)

:thumbup: you won't regret it!

For the OP. A still air box is pretty much useless without being used in a room with fairly still air. By doing so you can have arm holes that are 6"+ without having to worry about drafts. Larger arm holes so when you put your arms in and out, it won't move any air. That and your skinny arms with very little excess material around them, or no material, will also move very little air around. If you do it right, and you have very little air moving around.. the only thing you have to worry about is shit hovering over open media (just your flamed tools are allowed over it). If you do so, you will see no contams.

I could imagine a well sealed glove box out performing a still air box in an open environment with lots of air movement. But if you follow the still air principle, you can have a flame going outside your box, move your arm in with no air movement around the arm holes, flame all your tools when needed, and have a Much better success rate than a glove box. :2cents:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (08/26/15 12:43 PM)


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OfflinePinPornProducer
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Re: Glovebox vs SAB [Re: Mad Season]
    #22146491 - 08/26/15 01:07 PM (8 years, 5 months ago)

Skinny arms? Lol


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InvisibleMad Season
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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22146504 - 08/26/15 01:09 PM (8 years, 5 months ago)

You know what I mean :lol: a lot less of a surface area moving than gloves.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinePinPornProducer
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Re: Glovebox vs SAB [Re: Mad Season]
    #22146584 - 08/26/15 01:32 PM (8 years, 5 months ago)

lol


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Offlinekushroom
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Re: Glovebox vs SAB [Re: Mad Season]
    #22146848 - 08/26/15 02:27 PM (8 years, 5 months ago)

Quote:

Mad Season said:
:thumbup: you won't regret it!

For the OP. A still air box is pretty much useless without being used in a room with fairly still air. By doing so you can have arm holes that are 6"+ without having to worry about drafts. Larger arm holes so when you put your arms in and out, it won't move any air. That and your skinny arms with very little excess material around them, or no material, will also move very little air around. If you do it right, and you have very little air moving around.. the only thing you have to worry about is shit hovering over open media (just your flamed tools are allowed over it). If you do so, you will see no contams.

I could imagine a well sealed glove box out performing a still air box in an open environment with lots of air movement. But if you follow the still air principle, you can have a flame going outside your box, move your arm in with no air movement around the arm holes, flame all your tools when needed, and have a Much better success rate than a glove box. :2cents:




Last time i tried that i blow up the inside of my SAB nd caught a paper towel soaked in iso on fire almost taking out the living room carpet lol, i think i need to switch to a flow hood cause im not to good with fire i guess, oh well all that matters is my agar is clean now :smile:


--------------------
:/



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Offlinethebug76
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Re: Glovebox vs SAB [Re: kushroom]
    #22146986 - 08/26/15 02:53 PM (8 years, 5 months ago)

Quote:

kushroom said:
Quote:

Mad Season said:
:thumbup: you won't regret it!

For the OP. A still air box is pretty much useless without being used in a room with fairly still air. By doing so you can have arm holes that are 6"+ without having to worry about drafts. Larger arm holes so when you put your arms in and out, it won't move any air. That and your skinny arms with very little excess material around them, or no material, will also move very little air around. If you do it right, and you have very little air moving around.. the only thing you have to worry about is shit hovering over open media (just your flamed tools are allowed over it). If you do so, you will see no contams.

I could imagine a well sealed glove box out performing a still air box in an open environment with lots of air movement. But if you follow the still air principle, you can have a flame going outside your box, move your arm in with no air movement around the arm holes, flame all your tools when needed, and have a Much better success rate than a glove box. :2cents:




Last time i tried that i blow up the inside of my SAB nd caught a paper towel soaked in iso on fire almost taking out the living room carpet lol, i think i need to switch to a flow hood cause im not to good with fire i guess, oh well all that matters is my agar is clean now :smile:





Oh shit. Don't flame inside the box. Alcohol fumes are very combustible. Flame outside the SAB then put it back inside.


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Offlinekushroom
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Re: Glovebox vs SAB [Re: thebug76]
    #22147021 - 08/26/15 02:57 PM (8 years, 5 months ago)

Haha yeah i wasnt thinking that when i did, had a couple drinks in me nd was going ham on the dabs lol


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:/



All submitted posts are by Someone Who Isn't Me (SWIM) -  and in any event are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.


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InvisibleInocuole
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Re: Glovebox vs SAB [Re: kushroom]
    #22147879 - 08/26/15 05:48 PM (8 years, 5 months ago)

Could just use soapy water and you wouldn't have fire.


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Invisibleblackdust
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Re: Glovebox vs SAB [Re: Inocuole]
    #22147969 - 08/26/15 06:02 PM (8 years, 5 months ago)

Srirachi


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