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OfflinePinPornProducer
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Glovebox vs SAB
    #22142875 - 08/25/15 08:08 PM (8 years, 5 months ago)

I keep hearing about gloveboxes being a bad option for mycology and that sabs are the best. What are the downsides of using a gb? All I've ever used is a glovebox and I have had much success using it. I know flame sterilizing inside an gb is a no no so I just made a flame port in the side of the box. I just keep the torch outside the box and just stick the scalpel through the hole and into the flame. I can see a sab being a little more user friendly but as far as contams go I would think the minimum outside air exposure would be the best option.
 


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OfflineGrim767
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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22143071 - 08/25/15 08:40 PM (8 years, 5 months ago)

Never used a GB but I'd imagine getting hands in and out of gloves would be royal pain. I use a SAB and I find it convenient.


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Re: Glovebox vs SAB [Re: Grim767]
    #22143109 - 08/25/15 08:47 PM (8 years, 5 months ago)

I think there abt equal I'll take the gloves out of mine to do g2g but have the gloves attached when doin agar and syringe work.


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InvisibleInocuole
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Re: Glovebox vs SAB [Re: fastlearner]
    #22143379 - 08/25/15 09:50 PM (8 years, 5 months ago)

If you have a flame port, and you have gloves attached, then you're pulling in air through that hole when you move your arms around.  That's reason enough for me, case closed.

Plus I seen the vets here doing their SAB work with their bare hands or just latex gloves.  Would rather aspire to that, knowing it can work. :shrug:


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OfflinePinPornProducer
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Re: Glovebox vs SAB [Re: Inocuole]
    #22143456 - 08/25/15 10:07 PM (8 years, 5 months ago)

I've been thinking about switching to an sab but I'm just Leary about huge open arm holes. I live in a semi kinda country area and mold spores are in an abundance and I do all my work on my coffee table. You think my success rate will stay the same using an sab?


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OfflinePussyFart
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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22143471 - 08/25/15 10:11 PM (8 years, 5 months ago)

Quote:

PinPornProducer said:
I keep hearing about gloveboxes being a bad option for mycology and that sabs are the best. What are the downsides of using a gb?



This has already been discussed to death, to the fact that it is boring......you could have just done a simple search......

The gloves are attached to the box, meaning it makes tools very hard to get in and out....

The gloves being attached will create a semi sealed vessel, and create drafts when you move your arms that come in and out of the imperfect seals around the box, negating the "still air environment".

If you flame sterilize inside of the box you create heat currents, negating the "still air environment"......
Quote:

PinPornProducer said:
I've been thinking about switching to an sab but I'm just Leary about huge open arm holes.



Use tyvek sleeves......they keep the holes closed.....


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Offlinebluegill
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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22143495 - 08/25/15 10:16 PM (8 years, 5 months ago)

I saw the thread by Roadkill with his SAB and just decided to do the same. Why take extra precaution if it is not necessary? I just built my SAB Sunday night using the PVC drain/caps. :thumbup:


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Re: Glovebox vs SAB [Re: PussyFart]
    #22143666 - 08/25/15 10:46 PM (8 years, 5 months ago)

I kinda think tyvek sleeves are counterproductive in that they help instill the fear of the arm holes. :shrug:

It's like half-assing the getting over it.


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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22143704 - 08/25/15 10:54 PM (8 years, 5 months ago)

Quote:

PussyFart said:
The gloves being attached will create a semi sealed vessel, and create drafts when you move your arms that come in and out of the imperfect seals around the box, negating the "still air environment".




True in most cases. But if you really wanna spend the time and money to build a serious SAB that is 100% sealed it'll work. I'm talking sealed wood box, not a plastc tub. Real labs do it. Still have the problem of moving tools in and out, but you could have a shitload of PC'd tools in your box instead.

But an at-home version will cost almost as much as a flowhood in most cases, making it pointless for most of us. If you can get materials for free it'd be worth it. Except it wouldn't IMO, cause those free materials could make a plenum.

Quote:

PinPornProducer said: I live in a semi kinda country area and mold spores are in an abundance and I do all my work on my coffee table. You think my success rate will stay the same using an sab?




Or go up. I basically live in a rainforest. I use a SAB with 99%+ success, and so do a couple other growers I know from around here. When I was having issues with my schmuvbox giving me 90% contams rates, my buddy with a SAB was sailing along growing lbs.


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i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineKizzle
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Re: Glovebox vs SAB [Re: 36fuckin5]
    #22144525 - 08/26/15 04:52 AM (8 years, 5 months ago)

Quote:

The gloves being attached will create a semi sealed vessel, and create drafts when you move your arms that come in and out of the imperfect seals around the box, negating the "still air environment".




Quote:

Use tyvek sleeves......they keep the holes closed.....



Wait a minute. So when you have the gloves attached you're creating a semi sealed vessel but when you close the arm holes with the tyvek sleeves you're not?


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Re: Glovebox vs SAB [Re: Kizzle]
    #22144611 - 08/26/15 06:17 AM (8 years, 5 months ago)

That's under the assumption that tyvek is breathable enough that movement of those sleeves won't cause a positive or negative pressure in the SAB.

I don't really care to operate under that assumption, more stuff to flap around and make wind. :shrug:


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OfflinePussyFart
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Re: Glovebox vs SAB [Re: Inocuole]
    #22144952 - 08/26/15 08:26 AM (8 years, 5 months ago)

It actually works really well......I have a pair attached to the box, and I wear a pair on my arms.....this is just to stop dead skin cells from my arms from falling into the box....I would not back the idea if it didn't work fantastically....


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OfflinePinPornProducer
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Re: Glovebox vs SAB [Re: PussyFart]
    #22144972 - 08/26/15 08:29 AM (8 years, 5 months ago)

Tyvek sleeves attached to the box is basically a glove box...


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OfflinePussyFart
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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22145021 - 08/26/15 08:44 AM (8 years, 5 months ago)

No, it isn't....the gloves do not form a seal, they are breathable, and the elestc ends have been cut off so it makes it easier to work out of.....it's perfect.


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OfflinePussyFart
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Re: Glovebox vs SAB [Re: PussyFart]
    #22145038 - 08/26/15 08:48 AM (8 years, 5 months ago)

   


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Offlinespacechildo
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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22145045 - 08/26/15 08:50 AM (8 years, 5 months ago)

whats the reasoning behind the hole with the flame outside? it will just stir up air, a flame needs air to live
and heat makes heat convections..


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OfflinePinPornProducer
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Re: Glovebox vs SAB [Re: spacechildo]
    #22145083 - 08/26/15 08:59 AM (8 years, 5 months ago)

It works :shrug:


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OfflinePinPornProducer
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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22145122 - 08/26/15 09:06 AM (8 years, 5 months ago)

In the last year I've lost maybe 5 jars and I make alot of spawn jars and my agar plates are clean. I've got 30 plates right now with no signs of contams and any contam on a plate I have seen is from original spore drop and by 2nd transfer it's clean.


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Offlinespacechildo
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Re: Glovebox vs SAB [Re: PinPornProducer]
    #22145286 - 08/26/15 09:42 AM (8 years, 5 months ago)

SABs are proven to work while flames are proven to make air movements,
what is it that makes you doubt the SABs function?
there's a lot of stuff in this hobby that "works" but it doesnt mean you should do it :shrug:


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OfflinePinPornProducer
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Re: Glovebox vs SAB [Re: spacechildo]
    #22145367 - 08/26/15 09:54 AM (8 years, 5 months ago)

Idk man, the whole taking your arms in and out to flame shit kinda worries me I guess.


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