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Jutter
Stranger
Registered: 06/17/14
Posts: 3
Last seen: 7 years, 10 months
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Alternatives to flaming needles?
#22141831 - 08/25/15 04:52 PM (8 years, 5 months ago) |
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Aloha, Is it a viable option to steam sterilize or pressure cook used syringe needles? I have used needles straight out of the wrapper without flaming in a sealed G\B and had good results. When I remove the gloves from the box and flame the used needles things go south. So I guess what I would do is have one sterilized needle per jar and swap them inbetween. Any thoughts or ideas regarding this?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Alternatives to flaming needles? [Re: Jutter]
#22141871 - 08/25/15 05:00 PM (8 years, 5 months ago) |
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Make a still air box not a glove box. Gloves attached to the box is a no no for what kind of sterile work we are doing.
You could make 8inch diameter arm holes and just wear gloves and your success likely goes up substantially
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Jutter
Stranger
Registered: 06/17/14
Posts: 3
Last seen: 7 years, 10 months
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Re: Alternatives to flaming needles? [Re: bodhisatta]
#22141982 - 08/25/15 05:16 PM (8 years, 5 months ago) |
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That is what I have but when I remove the gloves and flame the needle I have bad luck
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Alternatives to flaming needles? [Re: Jutter]
#22142004 - 08/25/15 05:19 PM (8 years, 5 months ago) |
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The bad luck is in your syringe already then. Nothing is more sterile than red hot metal. So the problem isn't the seconds it takes to bring it back in.
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Jutter
Stranger
Registered: 06/17/14
Posts: 3
Last seen: 7 years, 10 months
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Re: Alternatives to flaming needles? [Re: bodhisatta]
#22142290 - 08/25/15 06:14 PM (8 years, 5 months ago) |
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That makes sense.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 12 minutes
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Re: Alternatives to flaming needles? [Re: Jutter]
#22142784 - 08/25/15 07:53 PM (8 years, 5 months ago) |
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If you do open lid inoculations in a SAB with the needle you only need to flame sterilize once unless it touches something.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Alternatives to flaming needles? [Re: bodhisatta]
#22143860 - 08/25/15 11:28 PM (8 years, 5 months ago) |
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Quote:
bodhisatta said:Nothing is more sterile than red hot metal. So the problem isn't the seconds it takes to bring it back in.
This. Heat sterilizes. Chemicals sanitize.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Alternatives to flaming needles? [Re: Jutter]
#22143888 - 08/25/15 11:34 PM (8 years, 5 months ago) |
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Quote:
Jutter said:
Is it a viable option to steam sterilize or pressure cook used syringe needles?
You answered your own question though. Needles need to be sterilized so if you sterilize them but it sounds like the needle is sterile considering you either have been using a flame or autoclave
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: Alternatives to flaming needles? [Re: Jutter]
#22144041 - 08/26/15 12:17 AM (8 years, 5 months ago) |
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No need to flame the needle for every jar. If it was sterilized in the PC, it won't need to be flamed unless it was exposed to the open air or touched something, though it's not a bad idea. I generally do many consecutive jars without flaming, and just try to be efficient, keep my hands from getting over the jars. If a sufficient volume of spores land right on the needle tip between jars to cause contamination, opening the jar lids would screw you infinitely harder since the surface area of open grain is so massive by comparison. I reflame the needle before storage, if I bonk something, get my hands over the needle somehow, set it down, or have a prolonged delay.
Attempting to exchange needles will have your gloved hands touching the syringe near the needle, exposing the contents of the syringe to the glovebox air, and is generally much less clean and will take longer.
Most likely any problems you had were randomly associated with outside flaming. Try to shut down outside air movement better if you're flaming outside the box.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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^ bad advice. Every inoculation is a contam vector. Every individual jar is a contam vector. If you make a mistake and pick up mold spores on one jar you'll transfer them to every jar after that until you re-sterilize your tool. In a 100% sterile environment this wouldn't even work reliably.
The best bet is to flame between each jar and use proper technique. If you're using a syringe and you open the lid to inoculate (same as you would with G2G) and the lid is never over the syringe tip, and the tip never touches the jar you can flame once. But if you set the syringe down to close the jar you have to flame it again.
Also, I can change tips without touching them. The package they come in is designed that way, or you wrap them in foil so they peel from the open end so you can not touch them. Work on your technique.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: Alternatives to flaming needles? [Re: 36fuckin5]
#22144187 - 08/26/15 01:11 AM (8 years, 5 months ago) |
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I assumed he was popping lids in the air box, not using ports. Can't imagine going to all the work of ports unless I didn't have an SAB. It's so easy to do cleanlid pops in SAB. You're absolutely right about flaming every time if you're punching through ports or poly. I even pop some of my old lids that do have ports cause it's just easier.
What I meant about changing the tips is not to say that it's impossilble or ever hard to do cleanly, just that I can't see how removing the tip with the syringe held facing up, breifly exposing the liquid, then lowing on another needle (which I believe would necessitate having your hand above the exposed liquid) could possibly be cleaner than flaming the needle.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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I still wouldn't flame a needle between every jar.
I used to flame them every third jar during the pf tek days. But that was because the needle directly touched substrate.
I have got a hundred bags growing right now that I have stuck the needle through the sides. Not one needle has been flamed and each has been used 15+ times now. All I do is pick am injection site well above grain level. Wipe the plastic with rubbing alcohol. Lay a strip of masking tape on the bag with the tape peeled back where I am going to insert the needle. Insert the 23g needle and squirt in an 1/8th-1/4th cc. Start to cover the needle with the tape while removing it. Double up the tape. Done! Sounds like a bunch of over kill. Oh, this is done in the middle of the kitchen.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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