Home | Community | Message Board

MRCA Tyroler Gluckspilze
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Substrate Bags   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: stareatclouds]
    #22142561 - 08/25/15 07:11 PM (8 years, 5 months ago)

Quote:

stareatclouds said:
Even if it's hard for crap to germinate in CVG, can't it colonize it all the same, and THEN germinate when you spawn? Sure, colonized grains will help fight it off, but it still seems worse.

Seems like giving it the heat treatment or pasteurizing properly again might be a safe bet.






Giving it another heat treatment is indeed the best bet if you're re-using coir.  Like I said, it's germination that doesn't like to happen on coir.  If some mold mycelium landed on there by chance, you better believe it would eat it up in a few days flat.  We just assume nothing is actually going to germinate since it would have had to germinate on something else rather than the coir. :shrug:

Still, might as well do your pasteurize/sterilize again.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: Inocuole]
    #22142580 - 08/25/15 07:13 PM (8 years, 5 months ago)

True. I usually use all my coir at once, but now I have a bucket of hydrated coir hanging out in a bucket. I'll definitely pasteurize it later just to be sure.


Extras: Filter Print Post Top
InvisibleRenegadeBlue
Gourmet
I'm a teapot

Registered: 06/08/15
Posts: 222
Loc: USA
Re: Blue's First Mono: A Grow Log [Re: stareatclouds]
    #22142760 - 08/25/15 07:49 PM (8 years, 5 months ago)

.


Edited by RenegadeBlue (09/19/16 09:58 AM)


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
    #22142812 - 08/25/15 07:58 PM (8 years, 5 months ago)

It's possible to accidentally isolate a clone further by taking out small wedges, if that answers your question.  It's also possible to get a big enough wedge that you're not really isolating much at all.  Putting MS to agar and then transferring once or twice before you put it to grain will usually give you a decent representation of the whole MS population, but there's no way to know for sure what you left behind.  Best to just get the growth that looks fast and use that.  So long as it's not too far from spore, all the growth should still be pretty heterozygous.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisibleRenegadeBlue
Gourmet
I'm a teapot

Registered: 06/08/15
Posts: 222
Loc: USA
Re: Blue's First Mono: A Grow Log [Re: Inocuole]
    #22143168 - 08/25/15 08:59 PM (8 years, 5 months ago)

.


Edited by RenegadeBlue (09/19/16 09:58 AM)


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
    #22143238 - 08/25/15 09:14 PM (8 years, 5 months ago)

Also, forgot to mention this, but clones don't always work as the standard definition would suggest.  A clone still has tons of sets of genetics, and they can produce different phenotypes in different conditions, or if you accidentally isolate away from the one strain in your clone culture that was actually producing the fruits you cloned for.  I haven't had the latter happen though.  I've taken some clones and grew them out and got nothing even remotely similar to the specimen I took from.  :shrug:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: Inocuole]
    #22143607 - 08/25/15 10:35 PM (8 years, 5 months ago)

MS (multi spore) will have tons of strains present. A clone (piece of tissue from mushroom body onto agar) will have far less strains present than MS due to anastomosis, but won't be a monoculture right off the bat. However, you can make transfers of the cloned tissue from the agar until you have monocultures (which is a LOT easier than starting from MS).

I think you are confusing terminology some. A clone is a piece of mushroom tissue, a transfer is simply taking a piece of mycelium in your agar and putting it to a new plate, an isolate/monoculture is a SINGLE strain from wherever on agar.

Due to anastomosis, you'll have less strains present from a clone, but you can still transfer til you hit a monoculture and wind up with a shitty culture (even though it came from a fruiter). Most people just keep the present strains around from a clone since that clone is pretty good already and then drop it into grains.

For now, jus worry about working with agar with sterile technique. Definitely keep researching, but isolating to monocultures can wait a bit in my opinion.

Drop spores to agar, make 2-3 transfers to ensure cleanliness, let that last transfer grow to the size of a silver dollar, and drop wedges into sterilized media. :smile:


Extras: Filter Print Post Top
InvisibleRenegadeBlue
Gourmet
I'm a teapot

Registered: 06/08/15
Posts: 222
Loc: USA
Re: Blue's First Mono: A Grow Log [Re: stareatclouds]
    #22144592 - 08/26/15 06:02 AM (8 years, 5 months ago)

.


Edited by RenegadeBlue (09/19/16 09:59 AM)


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
    #22144605 - 08/26/15 06:14 AM (8 years, 5 months ago)

Just don't worry about it, transfer as many times as you think is necessary to get the amount of clean culture you want to work with, with some to store in the fridge.  It's not like you're constantly in danger of ruining your good culture by transferring away from it, it's just a possibility.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisibleRenegadeBlue
Gourmet
I'm a teapot

Registered: 06/08/15
Posts: 222
Loc: USA
Re: Blue's First Mono: A Grow Log [Re: Inocuole]
    #22146101 - 08/26/15 12:13 PM (8 years, 5 months ago)

.


Edited by RenegadeBlue (09/19/16 09:59 AM)


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
    #22146131 - 08/26/15 12:19 PM (8 years, 5 months ago)

Don't crack it open. Vector for contams. Wait until you're going to transfer or inoculate whatever lol.

You can read my improve your sterile techniques link in my signature to see my techniques :shrug: but as far as isolating is concerned, listen to inoc and stare


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: Mad Season]
    #22146307 - 08/26/15 12:45 PM (8 years, 5 months ago)

Mad,

He's talking about his mini-monotub, not his agar plates,I believe.

I've always opened mine quickly to snap pictures or give the sub a smell. I thought that was common?


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: stareatclouds]
    #22146330 - 08/26/15 12:47 PM (8 years, 5 months ago)

Oh sorry I thought it was agar :lol: misread a little, my bad! yeah a mini mono is fine!


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
InvisibleRenegadeBlue
Gourmet
I'm a teapot

Registered: 06/08/15
Posts: 222
Loc: USA
Re: Blue's First Mono: A Grow Log [Re: Mad Season]
    #22146542 - 08/26/15 01:20 PM (8 years, 5 months ago)

.


Edited by RenegadeBlue (09/19/16 09:59 AM)


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
    #22146962 - 08/26/15 02:49 PM (8 years, 5 months ago)

It depends on the substrate you use for what to look for. I never really smell fresh mushrooms early on. Coir and vermiculite and gypsum don't smell like mushrooms. It just definitely shouldn't smell sweet or sour.


Extras: Filter Print Post Top
InvisibleRenegadeBlue
Gourmet
I'm a teapot

Registered: 06/08/15
Posts: 222
Loc: USA
Re: Blue's First Mono: A Grow Log [Re: stareatclouds]
    #22148295 - 08/26/15 06:53 PM (8 years, 5 months ago)

.


Edited by RenegadeBlue (09/19/16 10:00 AM)


Extras: Filter Print Post Top
Offlinetetherface
get in where you fit in
Male User Gallery


Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
    #22148336 - 08/26/15 07:01 PM (8 years, 5 months ago)

:thumbup::thumbup:good job bro!


--------------------


Extras: Filter Print Post Top
Offlineaerow.thefox
professional amatuer
 User Gallery


Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
    #22148358 - 08/26/15 07:09 PM (8 years, 5 months ago)

:thumbup:


--------------------
need a pc build list? i got you



Extras: Filter Print Post Top
InvisibleRenegadeBlue
Gourmet
I'm a teapot

Registered: 06/08/15
Posts: 222
Loc: USA
Re: Blue's First Mono: A Grow Log [Re: tetherface]
    #22148477 - 08/26/15 07:35 PM (8 years, 5 months ago)

.


Edited by RenegadeBlue (09/19/16 10:01 AM)


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
    #22148484 - 08/26/15 07:38 PM (8 years, 5 months ago)

Like.... 3-4 days?


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | Next >  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Substrate Bags   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* DB kit Growing log... Proteus 3,710 15 11/12/01 09:50 PM
by Proteus
* Grow Logs or data fungalrfarmer 601 2 05/13/03 11:47 AM
by bansheee
* Hydro-Pod newbie grow log
( 1 2 3 4 5 all )
thedm9 20,794 80 09/26/01 02:14 PM
by CACA
* grow log! phil2k1 2,617 5 03/23/02 08:41 PM
by phil2k1
* Blue's Poo (clean thread)
( 1 2 all )
mycofile 6,102 30 09/27/02 11:53 AM
by mycofile
* I want your logs! fr0zbnitz 2,456 8 07/18/01 07:31 PM
by BrownPastures
* substrate transfer?!?! dets 2,910 8 11/13/02 12:29 AM
by SixTango
* Grow Chamber Setup, few questions
( 1 2 all )
Ghosty 5,668 26 12/09/02 08:36 PM
by cheifdog24

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,674 topic views. 33 members, 246 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.011 seconds on 15 queries.