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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
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Quote:
stareatclouds said: Even if it's hard for crap to germinate in CVG, can't it colonize it all the same, and THEN germinate when you spawn? Sure, colonized grains will help fight it off, but it still seems worse.
Seems like giving it the heat treatment or pasteurizing properly again might be a safe bet.
Giving it another heat treatment is indeed the best bet if you're re-using coir. Like I said, it's germination that doesn't like to happen on coir. If some mold mycelium landed on there by chance, you better believe it would eat it up in a few days flat. We just assume nothing is actually going to germinate since it would have had to germinate on something else rather than the coir. 
Still, might as well do your pasteurize/sterilize again.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Blue's First Mono: A Grow Log [Re: Inocuole]
#22142580 - 08/25/15 07:13 PM (8 years, 5 months ago) |
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True. I usually use all my coir at once, but now I have a bucket of hydrated coir hanging out in a bucket. I'll definitely pasteurize it later just to be sure.
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RenegadeBlue
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Registered: 06/08/15
Posts: 222
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Edited by RenegadeBlue (09/19/16 09:58 AM)
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Inocuole
Scalpel of Evil's Bane



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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22142812 - 08/25/15 07:58 PM (8 years, 5 months ago) |
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It's possible to accidentally isolate a clone further by taking out small wedges, if that answers your question. It's also possible to get a big enough wedge that you're not really isolating much at all. Putting MS to agar and then transferring once or twice before you put it to grain will usually give you a decent representation of the whole MS population, but there's no way to know for sure what you left behind. Best to just get the growth that looks fast and use that. So long as it's not too far from spore, all the growth should still be pretty heterozygous.
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RenegadeBlue
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Re: Blue's First Mono: A Grow Log [Re: Inocuole]
#22143168 - 08/25/15 08:59 PM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 09:58 AM)
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Inocuole
Scalpel of Evil's Bane



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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22143238 - 08/25/15 09:14 PM (8 years, 5 months ago) |
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Also, forgot to mention this, but clones don't always work as the standard definition would suggest. A clone still has tons of sets of genetics, and they can produce different phenotypes in different conditions, or if you accidentally isolate away from the one strain in your clone culture that was actually producing the fruits you cloned for. I haven't had the latter happen though. I've taken some clones and grew them out and got nothing even remotely similar to the specimen I took from.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Blue's First Mono: A Grow Log [Re: Inocuole]
#22143607 - 08/25/15 10:35 PM (8 years, 5 months ago) |
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MS (multi spore) will have tons of strains present. A clone (piece of tissue from mushroom body onto agar) will have far less strains present than MS due to anastomosis, but won't be a monoculture right off the bat. However, you can make transfers of the cloned tissue from the agar until you have monocultures (which is a LOT easier than starting from MS).
I think you are confusing terminology some. A clone is a piece of mushroom tissue, a transfer is simply taking a piece of mycelium in your agar and putting it to a new plate, an isolate/monoculture is a SINGLE strain from wherever on agar.
Due to anastomosis, you'll have less strains present from a clone, but you can still transfer til you hit a monoculture and wind up with a shitty culture (even though it came from a fruiter). Most people just keep the present strains around from a clone since that clone is pretty good already and then drop it into grains.
For now, jus worry about working with agar with sterile technique. Definitely keep researching, but isolating to monocultures can wait a bit in my opinion.
Drop spores to agar, make 2-3 transfers to ensure cleanliness, let that last transfer grow to the size of a silver dollar, and drop wedges into sterilized media.
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RenegadeBlue
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Edited by RenegadeBlue (09/19/16 09:59 AM)
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Inocuole
Scalpel of Evil's Bane



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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22144605 - 08/26/15 06:14 AM (8 years, 5 months ago) |
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Just don't worry about it, transfer as many times as you think is necessary to get the amount of clean culture you want to work with, with some to store in the fridge. It's not like you're constantly in danger of ruining your good culture by transferring away from it, it's just a possibility.
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RenegadeBlue
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Re: Blue's First Mono: A Grow Log [Re: Inocuole]
#22146101 - 08/26/15 12:13 PM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 09:59 AM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22146131 - 08/26/15 12:19 PM (8 years, 5 months ago) |
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Don't crack it open. Vector for contams. Wait until you're going to transfer or inoculate whatever lol.
You can read my improve your sterile techniques link in my signature to see my techniques but as far as isolating is concerned, listen to inoc and stare
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Blue's First Mono: A Grow Log [Re: Mad Season]
#22146307 - 08/26/15 12:45 PM (8 years, 5 months ago) |
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Mad,
He's talking about his mini-monotub, not his agar plates,I believe.
I've always opened mine quickly to snap pictures or give the sub a smell. I thought that was common?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
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Oh sorry I thought it was agar misread a little, my bad! yeah a mini mono is fine!
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RenegadeBlue
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Re: Blue's First Mono: A Grow Log [Re: Mad Season]
#22146542 - 08/26/15 01:20 PM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 09:59 AM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22146962 - 08/26/15 02:49 PM (8 years, 5 months ago) |
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It depends on the substrate you use for what to look for. I never really smell fresh mushrooms early on. Coir and vermiculite and gypsum don't smell like mushrooms. It just definitely shouldn't smell sweet or sour.
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RenegadeBlue
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Edited by RenegadeBlue (09/19/16 10:00 AM)
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tetherface
get in where you fit in



Registered: 10/05/14
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22148336 - 08/26/15 07:01 PM (8 years, 5 months ago) |
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 good job bro!
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aerow.thefox
professional amatuer



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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22148358 - 08/26/15 07:09 PM (8 years, 5 months ago) |
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RenegadeBlue
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Re: Blue's First Mono: A Grow Log [Re: tetherface]
#22148477 - 08/26/15 07:35 PM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 10:01 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22148484 - 08/26/15 07:38 PM (8 years, 5 months ago) |
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Like.... 3-4 days?
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