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RenegadeBlue
Gourmet


Registered: 06/08/15
Posts: 222
Loc: USA
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Blue's First Mono *DELETED*
#22137314 - 08/24/15 07:59 PM (8 years, 5 months ago) |
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Post deleted by RenegadeBlueReason for deletion: .
Edited by RenegadeBlue (09/19/16 10:07 AM)
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22137358 - 08/24/15 08:07 PM (8 years, 5 months ago) |
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 I'm not being a smart a$$... I'm just curious... Why did you put left over CVG for casing in the fridge?... I usually just leave it on a shelve in a sealed bucket or jar for later use. Good Vibes on your project bro!
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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RenegadeBlue
Gourmet


Registered: 06/08/15
Posts: 222
Loc: USA
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Re: Blue's First Mono: A Grow Log [Re: Darkhome]
#22137374 - 08/24/15 08:12 PM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 09:33 AM)
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22137397 - 08/24/15 08:20 PM (8 years, 5 months ago) |
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No worries...I hope you get a ton of fruits... I'm still somewhat new to bulk myself, but I've read... Alot... If there is anything I can help you with... Feel free to ask. Frank's teks are great...they started me in bulk. I'm about to try whole oats next, but I've had alot of success with WBS. I find users I like and read all their main posts...and if you need help, just ask ...this place and its users are AMAZING!
 I'll send you 5 Shrooms ...you seem to be off on the right foot.
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
Edited by Darkhome (08/24/15 08:21 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Blue's First Mono: A Grow Log [Re: Darkhome]
#22137439 - 08/24/15 08:29 PM (8 years, 5 months ago) |
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I challenge you to get something to grow on CVG without putting grains in it. Shit sits for months and won't mold over at room temp, usually in the same container I prepped it in. Might be a good idea to get it to field capacity again and heat it up once more but for the most part things don't like to germinate on coir. I just leave mine in the bag I prep it in, and keep it twist-tied.
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RenegadeBlue
Gourmet


Registered: 06/08/15
Posts: 222
Loc: USA
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Re: Blue's First Mono: A Grow Log [Re: Darkhome]
#22137442 - 08/24/15 08:29 PM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 09:35 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22137455 - 08/24/15 08:31 PM (8 years, 5 months ago) |
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You're always at risk from things like that. Anything that you're not 100% sure isn't a risk, is by default, a risk. No way to tell you for sure other than use your best judgment, maybe post pics of the cakes in question if you're worried. It's not a whole lot more dangerous than spawning grains that were inoculated with MS.
Agar is the solution to all those woes though, and it's pretty easy to get into.
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Re: Blue's First Mono: A Grow Log [Re: Inocuole]
#22137528 - 08/24/15 08:45 PM (8 years, 5 months ago) |
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-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Blue's First Mono: A Grow Log [Re: Darkhome]
#22137529 - 08/24/15 08:45 PM (8 years, 5 months ago) |
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RenegadeBlue
Gourmet


Registered: 06/08/15
Posts: 222
Loc: USA
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Re: Blue's First Mono: A Grow Log [Re: Inocuole]
#22137532 - 08/24/15 08:46 PM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 09:56 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22137565 - 08/24/15 08:53 PM (8 years, 5 months ago) |
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There is no "in general" There could be one contaminant spore, or 5000, in the syringe. I suppose it's possible there could be none, but I wouldn't count on it.
It's all totally random. There's probably nothing wrong there but there's not really a way to know for sure. MS can work a lot of the time but when it doesn't, you probably won't even see it coming. That's why growing in 2D on agar is preferable.
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RenegadeBlue
Gourmet


Registered: 06/08/15
Posts: 222
Loc: USA
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Re: Blue's First Mono: A Grow Log [Re: Inocuole]
#22137579 - 08/24/15 08:59 PM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 09:56 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22137585 - 08/24/15 09:00 PM (8 years, 5 months ago) |
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There is risk of a contam showing up from the time you inoculate (Or before if you didn't sterilize correctly),all the way until the time you finally toss the thing because it's not producing mushrooms anymore. You can't let your guard down really.
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RenegadeBlue
Gourmet


Registered: 06/08/15
Posts: 222
Loc: USA
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Re: Blue's First Mono: A Grow Log [Re: Inocuole]
#22137610 - 08/24/15 09:06 PM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 09:56 AM)
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22137714 - 08/24/15 09:27 PM (8 years, 5 months ago) |
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I inoculated my first two bulk grows with a MS syringe (in a SAB), they worked out fine... But now that I found Agar I will never go back...who knows if I had dropped the spores on Agar, I might have had even better results. Agar gives you the piece of mind of "knowing" you have a clean culture instead of "hoping"...it also opens up a whole new realm of possibilities for your projects.... Check out these links about Agar...they will show you the "why" and the "how"
http://www.shroomery.org/forums/showflat.php/Number/21176491#21176491
Cloning...
http://www.shroomery.org/forums/showflat.php/Number/17784996/
And isolating...
http://www.shroomery.org/forums/showflat.php/Number/18430998/fpart/all/vc/1
I'm making pasty's plates as we speak...
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
Edited by Darkhome (08/24/15 09:30 PM)
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RenegadeBlue
Gourmet


Registered: 06/08/15
Posts: 222
Loc: USA
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Re: Blue's First Mono: A Grow Log [Re: Darkhome]
#22138985 - 08/25/15 06:43 AM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 09:57 AM)
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22139022 - 08/25/15 06:56 AM (8 years, 5 months ago) |
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Quote:
RenegadeBlue said: Thanks for all this awesome info. You're the man! Now to do a ton more reading haha
No problem... That's what we are here for ...to learn from each other Keep reading bro, I learn something new here almost every day.
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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RenegadeBlue
Gourmet


Registered: 06/08/15
Posts: 222
Loc: USA
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Re: Blue's First Mono: A Grow Log [Re: Darkhome]
#22142379 - 08/25/15 06:31 PM (8 years, 5 months ago) |
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Edited by RenegadeBlue (09/19/16 09:57 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22142504 - 08/25/15 06:59 PM (8 years, 5 months ago) |
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Quote:
RenegadeBlue said: Do I just cut it and drop it in the grain jars in a still air box?
Yes. Exactly like that. If you can transfer from agar to agar, you have the skills necessary to transfer to grain. I don't know of any teks specifically for that part since you can kind of extrapolate your agar skills and figure it out. Loosen both lids, cut a wedge, lift the grain lid, drop the wedge in, replace it, all while not being too slow or too fast.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Blue's First Mono: A Grow Log [Re: RenegadeBlue]
#22142534 - 08/25/15 07:05 PM (8 years, 5 months ago) |
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Quote:
Inocuole said: I challenge you to get something to grow on CVG without putting grains in it. Shit sits for months and won't mold over at room temp, usually in the same container I prepped it in. Might be a good idea to get it to field capacity again and heat it up once more but for the most part things don't like to germinate on coir. I just leave mine in the bag I prep it in, and keep it twist-tied.
Even if it's hard for crap to germinate in CVG, can't it colonize it all the same, and THEN germinate when you spawn? Sure, colonized grains will help fight it off, but it still seems worse.
Seems like giving it the heat treatment or pasteurizing properly again might be a safe bet.
Quote:
RenegadeBlue said: Hey man another quick question here. I read through most of the links u provided about agar and also did a little searching. All of the articles I've found seem to stop at the point where u have a colonized agar plate. But once I have the ideal monoculture how do I get it in to my grain jars? Do I just cut it and drop it in the grain jars in a still air box? Any useful posts/teks you might be able to refer me to for this process? Thanks in advance!
A monoculture is not really "ideal" for cubes, per se. If you somehow wound up with an insanely badass one, then sure, that's nice. But it takes a LOT of work to get there.
Like, it's work to get to an isolate/monoculture itself, and then you have to fruit it to see if it even performs. There's no guarantee the strain you're isolating is even a fruiter. If you have the time and inclination, you can isolate like 10+ strains, fruit them all, and see what happens.
Do a site search for anastomosis, isolating monocultures, MS Vs. isolates, etc. and you'll get some good information.
Here's PussyFart taking 10 isolates of PE (Penis Envy) and you can see how some perform and some don't. It's up to you if it's worth isolating or just usign MS.
Many very experienced growers just stay with MS since there's so many spores/strains, your odds of great flushes are always pretty decent.
Anyway, there's a few things to do once your spores or transfers have germinated and spread on the agar plates. Yes, you often just cut it out and drop it on sterilized grains. You can also drop it into sterilized water and blend it up to make a liquid inoculant. You can also use it to make a liquid culture as well. But cutting them out and dropping to grains is the most common.
Hope this helps.
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