Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate, Injection Grain Bag

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineDondematias
Stranger
 User Gallery
Registered: 08/24/15
Posts: 9
Last seen: 8 years, 3 months
White spots on grain, anybody knows what these are?
    #22137271 - 08/24/15 07:52 PM (8 years, 5 months ago)

These white spots apeared on my grain four days after inoculation, no myc has grown yet.



Extras: Filter Print Post Top
OfflineBrennus
Student of Life
Male User Gallery


Registered: 05/31/08
Posts: 3,297
Loc: SE United States Flag
Last seen: 5 years, 10 months
Re: White spots on grain, anybody knows what these are? [Re: Dondematias]
    #22137932 - 08/24/15 10:18 PM (8 years, 5 months ago)

Wrong forum for this.

That being said, looks like yeast to me especially if its been a few days and you haven't seen any mycelium yet.


Extras: Filter Print Post Top
OfflineDarkhome
•Shaman•N•Training•
Male User Gallery


Registered: 07/10/15
Posts: 517
Loc: United States Flag
Last seen: 7 years, 1 month
Re: White spots on grain, anybody knows what these are? [Re: Brennus]
    #22137992 - 08/24/15 10:32 PM (8 years, 5 months ago)

What did you inoculate with ...MS Syringe?


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: Darkhome]
    #22138459 - 08/25/15 01:39 AM (8 years, 5 months ago)

Definitely looks like yeast. I'd go dump it outside. If you inoculated with MS Syringe, you should learn agar immediately by Googling Pasty's Easy Agar Tek.


Extras: Filter Print Post Top
OfflineDondematias
Stranger
 User Gallery
Registered: 08/24/15
Posts: 9
Last seen: 8 years, 3 months
Re: White spots on grain, anybody knows what these are? [Re: stareatclouds]
    #22138814 - 08/25/15 04:57 AM (8 years, 5 months ago)

Yes I used MS Syringe. It was my first time making one, must have not done a good job at cleanliness. Thanks for the advice, will throw away and start again.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: Brennus]
    #22139063 - 08/25/15 07:10 AM (8 years, 5 months ago)

Quote:

Brennus said:
Wrong forum for this.

That being said, looks like yeast to me especially if its been a few days and you haven't seen any mycelium yet.



:facepalm: no its not. This is the most correct place for this.

Looks like gypsum to me. Shitty pic tho

Don't throw it early because of shitty advice. At least wait to know 100% its fucked


Extras: Filter Print Post Top
Offlinekosmokratorshaman
Cosmic Creator
Male User Gallery

Registered: 05/13/15
Posts: 1,206
Last seen: 7 years, 4 months
Re: White spots on grain, anybody knows what these are? [Re: bodhisatta]
    #22139427 - 08/25/15 07:40 AM (8 years, 5 months ago)

:whathesaid:


--------------------
I am.


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: Dondematias]
    #22140831 - 08/25/15 01:12 PM (8 years, 5 months ago)

Quote:

Dondematias said:
Yes I used MS Syringe. It was my first time making one, must have not done a good job at cleanliness. Thanks for the advice, will throw away and start again.




Did you use gypsum in your soak or simmer?


Extras: Filter Print Post Top
OfflineDondematias
Stranger
 User Gallery
Registered: 08/24/15
Posts: 9
Last seen: 8 years, 3 months
Re: White spots on grain, anybody knows what these are? [Re: stareatclouds]
    #22141013 - 08/25/15 02:09 PM (8 years, 5 months ago)

No gypsum was used at all.


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: Dondematias]
    #22141152 - 08/25/15 02:43 PM (8 years, 5 months ago)

I'd say yeast then. You should research agar ASAP, brother. It's super helpful and will avoid stuff like this a lot of the time.


Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: stareatclouds]
    #22141222 - 08/25/15 02:58 PM (8 years, 5 months ago)

Looks like yeast. Should smell like shit


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: tahoe]
    #22141292 - 08/25/15 03:15 PM (8 years, 5 months ago)

I believe I just tossed out 4 jars with yeast on them. I shook them up and when I opened it, a layer of dust or whatever rose off of it (was outside). Yeast would do that right?


Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: stareatclouds]
    #22141307 - 08/25/15 03:17 PM (8 years, 5 months ago)

Yeast is dustless. Or at least what I have been calling yeast is. It's slimy and smells like rotten v.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: tahoe]
    #22141344 - 08/25/15 03:24 PM (8 years, 5 months ago)

Hmm, probably just leftover shit from the grains (or them smacking into each other from the shake). Nothing looked slimy, pretty dry actually. I am sure it was yeast though.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: tahoe]
    #22141546 - 08/25/15 04:00 PM (8 years, 5 months ago)

Quote:

tahoe said:
Yeast is dustless. Or at least what I have been calling yeast is. It's slimy and smells like rotten v.



there's 100s of yeasts that all will have different morphology. some of them make pseudo hyphae and some of them like Candida albicans can make hyphae, pseudo hyphae, and also act yeast like.

some yeasts are dry and powdery some will be more wet/slime like.

what's on those grains if it is yeast is actually probably C.albicans


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: White spots on grain, anybody knows what these are? [Re: bodhisatta]
    #22141559 - 08/25/15 04:02 PM (8 years, 5 months ago)

:popcorn:

Why don't you detail us your grain prep and sterilization methods there OP?

Looks off if you didn't use gyp.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: taGyo]
    #22141602 - 08/25/15 04:10 PM (8 years, 5 months ago)

It's not bacteria so it's a sterile technique issue.
Either the inoculant or the inoculation
Mold would be killed even if you under sterilized but at least hit 170+ F


Extras: Filter Print Post Top
OfflineDondematias
Stranger
 User Gallery
Registered: 08/24/15
Posts: 9
Last seen: 8 years, 3 months
Re: White spots on grain, anybody knows what these are? [Re: bodhisatta]
    #22145996 - 08/26/15 11:46 AM (8 years, 5 months ago)

standard prep:

soak grain in purified water 24 hrs

simmer 20 mins, not boil

let drain for 30 mins

put in jars

close with lid (4 holes on lid for inoculation) and aluminum foil

PC for 80 mins

wait to cool down

inoculate

put in incubator at 27C


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: Dondematias]
    #22146072 - 08/26/15 12:04 PM (8 years, 5 months ago)

24 hours is likely too long. I believe most people recommend somewhere between 8-12 hours now since that standing water can activate endospores. I may be incorrect on the reasoning, but 24 hours is a long time.

I've never done MS with grains, but I don't think you don't need 4 holes. Are you covering the holes with anything later that can provide GE while simultaneously blocking contaminants from falling in (like we use SFDs for)?

You don't need an incubator. The temperature of the incubator is not what the inside of the substrate will be. It's way too hot for that and is likely helping contaminants get a foothold.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: stareatclouds]
    #22146141 - 08/26/15 12:22 PM (8 years, 5 months ago)

Don't use purified water use tap water.

4 hole lids are not for grain those are for PF/brf cakes

90m is the bare minimum at 15psi for grain


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: bodhisatta]
    #22146277 - 08/26/15 12:42 PM (8 years, 5 months ago)

Didn't even see purified water in that. :undecided:


Extras: Filter Print Post Top
OfflineBrennus
Student of Life
Male User Gallery


Registered: 05/31/08
Posts: 3,297
Loc: SE United States Flag
Last seen: 5 years, 10 months
Re: White spots on grain, anybody knows what these are? [Re: bodhisatta]
    #22146465 - 08/26/15 01:01 PM (8 years, 5 months ago)

Quote:

bodhisatta said:
Quote:

Brennus said:
Wrong forum for this.

That being said, looks like yeast to me especially if its been a few days and you haven't seen any mycelium yet.



:facepalm: no its not. This is the most correct place for this.

Looks like gypsum to me. Shitty pic tho

Don't throw it early because of shitty advice. At least wait to know 100% its fucked




This is why there's a contamination  forum. That's yeast. :cheers:


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: White spots on grain, anybody knows what these are? [Re: Brennus]
    #22146610 - 08/26/15 01:38 PM (8 years, 5 months ago)

The contamination forum on here sucks dick... Post your contamination q's in cultivation is pretty standard these days


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate, Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* grain and straw questions ParticleMan 1,046 3 03/20/02 08:05 PM
by ParticleMan
* Has anyone here had success casing rye grain? EPerson100 33,660 13 02/11/17 06:47 PM
by CubeSteakPoo
* tell me whats wrong with my grain (PICS) littlejohn747 1,367 15 08/14/04 12:12 PM
by OuchITripped
* How to prevent wet-spot? MOTH 1,707 14 11/28/04 03:53 PM
by Shdwstr
* Burnt grain spotted jars? MadHatR 964 3 11/27/04 02:00 AM
by MadHatR
* Whats the point of grains? Fd3000 1,311 11 07/24/02 09:04 PM
by phrozendata
* Seeds, Grains, and more ExtravagantDream 5,098 8 12/15/12 11:28 PM
by Mycopath666
* exploding grain question Zildjian 3,082 18 09/16/02 06:09 AM
by mycofile

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,336 topic views. 46 members, 253 guests and 37 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.01 seconds on 14 queries.