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invitro

Registered: 05/03/13
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Why does mycelium grow in LC but not in a waterlogged substrate?
#22135750 - 08/24/15 02:32 PM (8 years, 5 months ago) |
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Pretty straightforward question, I'm sure a lot of you have accidentally gotten a spawn jar/pf cake or whathaveyou overly wet and the mycelium will not colonize the soggy substrate. Yet that same mycelium will grow in a liquid culture.
??
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whitelights
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Registered: 11/25/11
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: invitro]
#22135768 - 08/24/15 02:35 PM (8 years, 5 months ago) |
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because the mycelium feeds off the small amounts of sugar/honey/whatever you use in your lc,i can only assume
-------------------- its that bitter-sweet-sour, electric-smooth-twang. everything you ever have, are. or will feel along with every emotion, joy, hate, love, fear or aspiration burning down your nerves and into the fabric of your place in this existence at ten thousand degrees above and below zero will you find yourself wondering if you've been dead or alive this whole time. being born over and over only to die over and over hoping the wheel stops in the same place it started when you spun it, and when it finally does and you can step back and take a nice deep breath you realize how beautiful life is, remember, wake up to the most beautiful day of your life every single day, its just the way.
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invitro

Registered: 05/03/13
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: whitelights]
#22135794 - 08/24/15 02:38 PM (8 years, 5 months ago) |
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Yea but if you grind up rice into flour and make a pf cake, and overhydrate the verm a little before sterilization of the cake, you can bet that water now has a healthy dose of nutrients. I have some substrate 50% coir / 50% WBS which I sterilized and inoculated. I hydrated the coir with the grain soak water so it has plenty of nutrients, but the bottom of the jar has some pooling of water and the myc won't grow into it. It's sparked my curiosity.
Edited by invitro (08/24/15 02:39 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: invitro]
#22135865 - 08/24/15 02:51 PM (8 years, 5 months ago) |
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Resistance. An LC has no resistance whereas a sopping bulk sub has lots. The myc will attempt to colonize it but has a much harder time pushing through. It's like the difference between swimming in water and swimming in molasses. Both are liquid.
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tahoe
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: Pastywhyte]
#22135927 - 08/24/15 03:08 PM (8 years, 5 months ago) |
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Like pasty said, it's too thick. I just trashed 4 bags that got water logged somehow. The bottom inch was soup. The mycelium was colonizing the thick stuff but it was going too slow
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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invitro

Registered: 05/03/13
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Last seen: 1 month, 21 days
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: Pastywhyte]
#22135942 - 08/24/15 03:12 PM (8 years, 5 months ago) |
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Is that resistance as in surface tension?
Or some other kind of resistance ? 
Tahoe are you referring to compaction?
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: tahoe]
#22135952 - 08/24/15 03:13 PM (8 years, 5 months ago) |
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Never mind me.. I just want to follow this.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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tahoe
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: JacobStorm]
#22135973 - 08/24/15 03:19 PM (8 years, 5 months ago) |
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It's just dense. But who knows. I think it will colonize it but Judy not that great. Mycelium fully colonize's agar. Agar is kind of dense.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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cronicr



Registered: 08/07/11
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Loc: Van Isle
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: tahoe]
#22135992 - 08/24/15 03:23 PM (8 years, 5 months ago) |
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Quote:
tahoe said: . Mycelium fully colonize's agar. Agar is kind of dense.
no it colonizes the surface lc's also get stirred to give air to them, we don't stir bulk subs
--------------------
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invitro

Registered: 05/03/13
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: cronicr]
#22136092 - 08/24/15 03:44 PM (8 years, 5 months ago) |
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Hmmm... In my experience LCs do just fine without stirring, it's how I do all my lcs, the small amount of oxygen that goes into the liquid seems to be enough for healthy growth (assuming you have some GE mechanism on your lc lid).
I think I can rule out compaction because I have pockets of sub less than an inch from the surface (top layer) of the sub... The parts without pooling of water are white and colonized the parts with show zero sign of growth.
Maybe if I wait long enough those pockets will colonize but I'm guessing not... And I don't want to birth with uncolonized pockets all over.
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tahoe
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: cronicr]
#22144294 - 08/26/15 01:51 AM (8 years, 5 months ago) |
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Quote:
cronicr said:
Quote:
tahoe said: . Mycelium fully colonize's agar. Agar is kind of dense.
no it colonizes the surface lc's also get stirred to give air to them, we don't stir bulk subs
It colonizes it for me. I have mycelium growing out all sides of an agar wedge well before the top layer recovers and starts colonizing the grain. I just remembered why that might be. I only use 10-12 grams of agar per liter of water. Maybe it's not as dense as yours. I use half the malt. Been using my 5,5,.5 formula forever. 5 grams agar, 5 grams malt, 500ml water.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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ShroominMe
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Re: Why does mycelium grow in LC but not in a waterlogged substrate? [Re: tahoe]
#22144444 - 08/26/15 03:42 AM (8 years, 5 months ago) |
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The lc has much less mass than a waterlogged sub. Mass means less movement which allows for the proliferation of anerobic contams.
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