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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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My 1st Mini Tubs (1st flush - success!!)
#22135551 - 08/24/15 01:50 PM (8 years, 5 months ago) |
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Hey everyone!! 
So I'm starting my first mono tubs soon following Payste's modified specs. Ill have 3 20qt tubs with 5 1\4" holes drilled and staggered at the top of the short sides, and 2 1\4" holes near the top of my substrate on the long sides. Ill be using Damion's 50\50 coir and verm only for my substrate and going with no casing layer.
Cambos were my choice and 'nocced up 7 qt WBS jars using Doc34's WBS prep tek. I plan on spawning 2 qts to each tub and using the 7th to spawn to agar using Pastye Plates to isolate in the event something happens with my other jars. I plan on making my plates with some grain water i saved from my boil. Aside from adding a little extra agar to the mix, is anything else needed beside the grain water? I assumed i could use it by itself...
Its been about 3 weeks and my jars are 85-90% colonized, though im worried they may be contaminated. The mycellium looks a little weak in some areas. This could be genetics or it could be contamination (cobweb?)...im not sure yet.
I have never documented any of my grows in the past, so i wanted to do that this time around. I will be posting pictures soon.
Any advice along the way, please feel free to express your opinions. Ive done PF tek and Mono Tubs sucessfully in the past so im pretty excited about these Mini Tubs
Edited by xachisonfire (09/11/15 08:53 AM)
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Im currently studying Pastye's Mono guide. Just wanted to follow this thread. Good luck man.
Oh and a picture of your suspected contaminate would help if at all possible.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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PoIIo
Stranger
Registered: 08/05/15
Posts: 83
Last seen: 8 years, 1 month
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Post some pictures for all of us. Go nuts on the pics.
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Re: My 1st Mini Tubs [Re: PoIIo]
#22136596 - 08/24/15 05:30 PM (8 years, 5 months ago) |
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Okay, so here's some pics...
These are the jars:
This is 3 weeks colonizing. I plan on giving them one more good shake...

The reason i suspect contamination is because of this. Its very "whispy". I have a feeling it's sticking to the glass like this because of the shaking for spreading noc points. But its everywhere in the jars. It could be genetics as well...it may just be weak myc growth like this:

This is my 1st run with grains so im just making sure everything is looking like it should. My BRF cakes int the past always looked fairly aggressive.
Here's the tub designs im going with. (Thanks Pastye)

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PoIIo
Stranger
Registered: 08/05/15
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Last seen: 8 years, 1 month
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Looking at the possible contam doesnt have me worried. I think its good genetics. Im sure the pros will confirm.
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WindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
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Re: My 1st Mini Tubs [Re: PoIIo]
#22136848 - 08/24/15 06:24 PM (8 years, 5 months ago) |
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Being newer take my advise with a grain of salt.
However that looks like good clean growth to me, I've seen tons of 'whispy' myc, but yours has a good rhizomorphic structure it looks like. Cobweb appears to be more random, grey in color.
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newrook
Sucks at bulk



Registered: 03/20/15
Posts: 657
Last seen: 3 months, 4 days
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That first jar closeup look's messier than I should be but I havn't used WBS a whole lot...
take the foil off the top of the jars.
lookin good! Good luck!
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  THROW AWAY YOUR SGFC
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WindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
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Re: My 1st Mini Tubs [Re: newrook]
#22136938 - 08/24/15 06:47 PM (8 years, 5 months ago) |
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Are you using SFDs? It looks like the foil isn't sitting as high up as it would with poly. Also record how long it takes to recover from the shake, and post it back here, that could be valuable determining whether or not you need to worry about contams.
[EDIT] Also in that first jar is it wispy all over in the jar like that, or just along the glass? Popping some kernels during the prep could spread some starch around in the jars, and the myc will definitely go for it.
Edited by WindWisperer (08/24/15 06:49 PM)
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Thats a very valid point. I did notice quite a few popped seeds. The starch being smeared along the glass during the shaking makes perfect sense. Thank you for that.
4 of the 7 jars have silicone injection ports AND polyfill. The other 3 have only polyfill. All jars have a layer of tyvek under the lids.
I tried to have a solid game plan before i started the whole process. I have ALOT of spare time at work, so i spend hours reading the forum. I've had changes mid stride because of what i've read. "This way is better, that way is better." My lids were one of those changes. I left the foil on for extra protection. Plus, high co2 levels promote good myc growth from what i understand so i didnt see an issue leaving the foil on.
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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I would take the foil off Your filters will block contaminants... You need a little gas exchange.
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Re: My 1st Mini Tubs [Re: Darkhome]
#22137351 - 08/24/15 08:06 PM (8 years, 5 months ago) |
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Just a quick update also:
I made my 1st payste plates this evening. I've been very excited to try agar. As i said in OP, i want to use my extra 7th jar to isolate an aggressive area of my myc from said jar. That way i can reap the benefits and cut alot of time and worries in my future endeavors.
I used 2 1/2 "heaping" tablespoons of ground agar agar strips mixed with about 1 1\2 cups of grain water from my boil. I also added a few drops of honey and some green food coloring so my cultures will be easier to view through these mini round containers.
  
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Re: My 1st Mini Tubs [Re: Darkhome]
#22137357 - 08/24/15 08:07 PM (8 years, 5 months ago) |
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The foil has been removed  Thanks!!
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
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Re: My 1st Mini Tubs [Re: Darkhome]
#22138096 - 08/24/15 11:03 PM (8 years, 5 months ago) |
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Cujllickduo



Registered: 06/13/15
Posts: 19,552
Loc: England
Last seen: 3 years, 6 months
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Its all looking good so far
keep us updated i hope it all works out for you man 
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WindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
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Looking good! Let us know how the plates work out for you, it seems some myc likes high nutrition value agar, and some like a little less nutrition. I am curious to see how your plate that utilized grain water works out.
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Update with a quick question: So i stated a week ago that i thought my jars were pretty close to being ready to spawn to my tubs. I gave them a good shake and still saw quite a bit of uncolonized grains. So i let them go for another week. The myc recovered in about a day and a half. So i looked again today to see how they were doing and they looked great. (See 1st picture.) All jars were 99% solid white. So to make sure it wasn't just on the surface again, i shook them. I have HUGE clups in these jars. I went after those bad boys with a rubber mallet and still couldn't break those big chunks apart. (See 2nd and 3rd pitures.) Anyway...this is my first run with grains so im just curious if they're actually good to go? Is yhe shaking process rubbing the myc off the grains and making it look like its not fully colonized? We're approaching the 5th week since inoculation.
The Payste plates came out great! I cant wait to get some transfers going, just waiting on these jars!
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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I'm extremely suspicious of this jar

Any time I've had empty space filled with apparent "structureless milkiness" it's been contaminated.
Also, you said two 1/4" wholes on the bottom? Should be more in the 1-1.5" diameter range, though the holes in your pic look like they are.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Quote:
Machiavelliavore said: I'm extremely suspicious of this jar

Any time I've had empty space filled with apparent "structureless milkiness" it's been contaminated.
Also, you said two 1/4" wholes on the bottom? Should be more in the 1-1.5" diameter range, though the holes in your pic look like they are.
Yeah, that 1/4" was a typo...i apologize. The drilled holes are 1 1/4" on the long sides.
That picture was taken about a week ago. And as i said, i shook them and the myc recovered just fine (it seems). I think the voided space there is because of the size of clumps in the jars. The milkyness i think may be an illusion. The myc being as whispy as it is could make it look cloudy...especially with not so great photos. I could be wrong though...you may be right. But after looking at them a couple times through the week, they seem to be doing pretty good...
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WindWisperer


Registered: 05/04/15
Posts: 333
Last seen: 9 months, 25 days
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I'd say do a grain to agar transfer, so you can learn agar, clean up some plates and have more ready to go just in case those jars are contaminated. Seriously agar has allowed me to get away from MS failures.
Go ahead and buy a tote that is about as wide (or wider) than your shoulder span, it helps to get one with a clear lid. Cut some arm holes in such a way to give you good maneuverability inside your still air box. I used a dremel to cut out a circle shape, then cleaned it up with a sanding disk. You can also use a coffee can that is all metal to melt a hole with a torch.
Doing this is the best way to have another project rolling that is almost ensured success just in case! Trust me you don't want to rely on one thing, and have it fail miserably, it hurts the feels.
[EDIT] I cannot confirm whether or not your jars are contaminated, sorry man. I would pluck a grain out of the jars when you spawn them to coir verm gypsum (or substrate of choice), and plop it right in the middle of your plate. Do this with a few, and have a few plates ready to go so just in case you get contams on your plates, you have a new one ready to accept a slice of clean colonized agar.
Edited by WindWisperer (08/30/15 04:30 PM)
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Quote:
WindWisperer said: I'd say do a grain to agar transfer, so you can learn agar, clean up some plates and have more ready to go just in case those jars are contaminated. Seriously agar has allowed me to get away from MS failures.
Go ahead and buy a tote that is about as wide (or wider) than your shoulder span, it helps to get one with a clear lid. Cut some arm holes in such a way to give you good maneuverability inside your still air box. I used a dremel to cut out a circle shape, then cleaned it up with a sanding disk. You can also use a coffee can that is all metal to melt a hole with a torch.
Doing this is the best way to have another project rolling that is almost ensured success just in case! Trust me you don't want to rely on one thing, and have it fail miserably, it hurts the feels.
[EDIT] I cannot confirm whether or not your jars are contaminated, sorry man. I would pluck a grain out of the jars when you spawn them to coir verm gypsum (or substrate of choice), and plop it right in the middle of your plate. Do this with a few, and have a few plates ready to go so just in case you get contams on your plates, you have a new one ready to accept a slice of clean colonized agar.
Thank you for the advice, it's highly appreciated! This is pretty much exactly what i went ahead and did. 
I went ahead and spawned 2 qts to my 50/50 CV substrate. The jars smelled amazing...fresh mushies!! Before spawning, i pulled out my SAB (which i already had built from inoculation ) and opened one of the jars to transfer to 4 Payste Plates. The coolest part about it is that i noticed a couple weeks ago that some grains were sticking to the bottom side of the tyvek under the lids from shaking. Sure enough there was some great looking growth there. So thats where my transfers came from. It was a breeze...pop both lids off, grab and done. So i now have 5 jars still colonizing, 4 Payste Plates colonizing, and 1 mini mono colonizing.
I just want to clone on some plates, so all i need is one successful grow!
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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FYI, Damion5050 coir verm substrate that people usually talk about is 80/20 coir verm. 50/50 will work fine, it just has a slightly less nutrition and costs a bit more.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Quote:
Machiavelliavore said: FYI, Damion5050 coir verm substrate that people usually talk about is 80/20 coir verm. 50/50 will work fine, it just has a slightly less nutrition and costs a bit more.
I was unaware of that ratio...i hope everything turns out ok. I imagine it might colonize faster with less nutes anyway as the myc "searches" for more food? My ratio was 3qts Verm, 3 qts Coir, and 2 qts of spawn in a 20 qt tub.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 20 hours
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Quote:
xachisonfire said:
Quote:
Machiavelliavore said: FYI, Damion5050 coir verm substrate that people usually talk about is 80/20 coir verm. 50/50 will work fine, it just has a slightly less nutrition and costs a bit more.
I was unaware of that ratio...
Did you even read the tek?
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Re: My 1st Mini Tubs [Re: PussyFart]
#22166538 - 08/30/15 08:40 PM (8 years, 4 months ago) |
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Quote:
PussyFart said:
Quote:
xachisonfire said:
Quote:
Machiavelliavore said: FYI, Damion5050 coir verm substrate that people usually talk about is 80/20 coir verm. 50/50 will work fine, it just has a slightly less nutrition and costs a bit more.
I was unaware of that ratio...
Did you even read the tek?
Yeah, i did read it. My mistake was not knowing the correct ammount of "qts" there are in one "brick" of coir. I didnt purchase my coir in a brick, it was already broken up in a bag...my own fault.
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Big Bear
Earf Child



Registered: 06/11/14
Posts: 5,415
Loc: In love, On time
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Quote:
Machiavelliavore said: I'm extremely suspicious of this jar

toss it. if it is suspicious dont spawn.
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Re: My 1st Mini Tubs [Re: Big Bear]
#22184232 - 09/03/15 06:32 PM (8 years, 4 months ago) |
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Update:
So i have all 3 mini's colonizing. Been about 4 days now and they're looking great. I have one more jar that ill be making an LC out of.
Here's how my Payste plates came out. This is also 4 days.
I took my boil water jar out of the freezer and let the glass get up to room temp. The boil water was still a frozen block inside the jar so i poured some warm tap water into the jar, swirled it around a few times and poured it out. Thats what i used to make my plates with.
So far, there haven't been any signs of contam. Needless to say, im having alot of fun doing all these different teks.
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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So my tubs have been colonizing for 6 days now, and i decided to sneak a peek. Just curious if this might be cobweb mold? If so, i saw RR say that the brown bottles of h2o2 in the US are already mixed at 3% and to just give it a spray. Anyone have experience with this? Thanks in advance!!
Close up

Further out
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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So i took my contam issues to the contamination forum and figured out what i needed to know.
Tub #1 has pinned prematurely. Either theres contamination somewhere, or my sub was too wet. The myc started knotting and i thought it might have been the start of trich. (Little white cottage cheese looking dots.)
I let it ride a couple days until i started noticing caps starting to form on the knots. I went ahead and fruited it. This is what it looked like this morning.


A decent pin set given the circumstances (in my opinion).
Ill dunk my sub with a little h2o2 after this flush if i dont notice any funky smells or discoloration just to be safe and see if i can get another flush out of it.
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Update:
Tub #1 is coming along very nicely.

Harvest day is fast approaching 
The other 2 were put in fruiting conditions 2 days ago. Hopefully they come out like tub #1.
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Here we go!!
First flush from tub #1. Not sure on the yield yet. Gonna swing by Wally World and grab a dehydrator at lunch and go harvest and start drying.
First mini tub = success

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PoIIo
Stranger
Registered: 08/05/15
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Last seen: 8 years, 1 month
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Did better than me! My shrooms all came out super tiny.
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Re: My 1st Mini Tubs [Re: PoIIo]
#22223513 - 09/11/15 06:34 PM (8 years, 4 months ago) |
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Quote:
PoIIo said: Did better than me! My shrooms all came out super tiny.
I had alot of smaller ones also...damn MS genetics. Only two decent sized ones...i decided to wait for my other 2 tubs (which have great pin sets right now) before i clone. Hoping ill get a better looking fruit from tub 2 or 3.
In comparison to yours though, i wouldnt say mine came out better...they're all just as good if you get fruits to store away. 
I got 500 grams wet weight. I figure mushies are roughly 90% water...so i was expecting roughly 10% (50 grams) dry weight. Those suckers shriveled up ALOT so im not so sure now...


They were a beautiful cluster a few hours ago...now it looks pitiful hahaha.
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uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
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Quote:
xachisonfire said: Here we go!!
First flush from tub #1. Not sure on the yield yet. Gonna swing by Wally World and grab a dehydrator at lunch and go harvest and start drying.
First mini tub = success


Looking good! What was your dry weight and how many quarts of spawn and bulk did you use per mono?
Edited by uninc4life2010 (09/12/15 02:49 AM)
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xachisonfire



Registered: 10/20/11
Posts: 70
Last seen: 6 years, 6 months
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Dry weight came out to 48 grams. Thats pretty close to what i guessed i was going to get.
Per tub i used 2 qts of WBS spawn to 4qts coir and 1qt verm.
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uninc4life2010
Unincorporated



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PoIIo
Stranger
Registered: 08/05/15
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Last seen: 8 years, 1 month
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Quote:
xachisonfire said:
Quote:
PoIIo said: Did better than me! My shrooms all came out super tiny.
I had alot of smaller ones also...damn MS genetics. Only two decent sized ones...i decided to wait for my other 2 tubs (which have great pin sets right now) before i clone. Hoping ill get a better looking fruit from tub 2 or 3.
In comparison to yours though, i wouldnt say mine came out better...they're all just as good if you get fruits to store away. 
I got 500 grams wet weight. I figure mushies are roughly 90% water...so i was expecting roughly 10% (50 grams) dry weight. Those suckers shriveled up ALOT so im not so sure now...


They were a beautiful cluster a few hours ago...now it looks pitiful hahaha.
I think you bring up the best point. In the end we grew our mushrooms and were rewarded for the months of reading this site. I have yet to even try shrooms and here I am ready to go. Make sure to keep us updated on those other tubs.
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Quote:
xachisonfire said: Here we go!!
First flush from tub #1. Not sure on the yield yet. Gonna swing by Wally World and grab a dehydrator at lunch and go harvest and start drying.
First mini tub = success


Nice job...keep rocking it bro!
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
Edited by Darkhome (09/16/15 11:26 PM)
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