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Offlinexachisonfire
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Registered: 10/20/11
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My 1st Mini Tubs (1st flush - success!!)
    #22135551 - 08/24/15 01:50 PM (8 years, 5 months ago)

Hey everyone!! :grin:

So I'm starting my first mono tubs soon following Payste's modified specs. Ill have 3 20qt tubs with 5 1\4" holes drilled and staggered at the top of the short sides, and 2 1\4" holes near the top of my substrate on the long sides. Ill be using Damion's 50\50 coir and verm only for my substrate and going with no casing layer.

Cambos were my choice and 'nocced up 7 qt WBS jars using Doc34's WBS prep tek. I plan on spawning 2 qts to each tub and using the 7th to spawn to agar using Pastye Plates to isolate in the event something happens with my other jars. I plan on making my plates with some grain water i saved from my boil. Aside from adding a little extra agar to the mix, is anything else needed beside the grain water? I assumed i could use it by itself...

Its been about 3 weeks and my jars are 85-90% colonized, though im worried they may be contaminated. The mycellium looks a little weak in some areas. This could be genetics or it could be contamination (cobweb?)...im not sure yet.

I have never documented any of my grows in the past, so i wanted to do that this time around. I will be posting pictures soon.

Any advice along the way, please feel free to express your opinions. Ive done PF tek and Mono Tubs sucessfully in the past so im pretty excited about these Mini Tubs :cool:


Edited by xachisonfire (09/11/15 08:53 AM)


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InvisibleJacobStorm
psychedelic cartel
I'm a teapot


Registered: 07/21/15
Posts: 1,499
Re: My 1st Mini Tubs [Re: xachisonfire]
    #22135690 - 08/24/15 02:22 PM (8 years, 5 months ago)

Im currently studying Pastye's Mono guide. Just wanted to follow this thread. Good luck man.

Oh and a picture of your suspected contaminate would help if at all possible.


--------------------
Rogger Rabbits Mushroom growing videos

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Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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OfflinePoIIo
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Re: My 1st Mini Tubs [Re: JacobStorm]
    #22135721 - 08/24/15 02:28 PM (8 years, 5 months ago)

Post some pictures for all of us. Go nuts on the pics.


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Offlinexachisonfire
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Re: My 1st Mini Tubs [Re: PoIIo]
    #22136596 - 08/24/15 05:30 PM (8 years, 5 months ago)

Okay, so here's some pics...

These are the jars:

This is 3 weeks colonizing. I plan on giving them one more good shake...




The reason i suspect contamination is because of this. Its very "whispy". I have a feeling it's sticking to the glass like this because of the shaking for spreading noc points. But its everywhere in the jars. It could be genetics as well...it may just be weak myc growth like this:



This is my 1st run with grains so im just making sure everything is looking like it should. My BRF cakes int the past always looked fairly aggressive.

Here's the tub designs im going with.
(Thanks Pastye)





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OfflinePoIIo
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Re: My 1st Mini Tubs [Re: xachisonfire]
    #22136828 - 08/24/15 06:20 PM (8 years, 5 months ago)

Looking at the possible contam doesnt have me worried. I think its good genetics. Im sure the pros will confirm.


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OfflineWindWisperer


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Re: My 1st Mini Tubs [Re: PoIIo]
    #22136848 - 08/24/15 06:24 PM (8 years, 5 months ago)

Being newer take my advise with a grain of salt.

However that looks like good clean growth to me, I've seen tons of 'whispy' myc, but yours has a good rhizomorphic structure it looks like. Cobweb appears to be more random, grey in color.


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Offlinenewrook
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Re: My 1st Mini Tubs [Re: WindWisperer]
    #22136862 - 08/24/15 06:27 PM (8 years, 5 months ago)

That first jar closeup look's messier than I should be but I havn't used WBS a whole lot...

take the foil off the top of the jars.

lookin good! Good luck!


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                                                                      THROW AWAY YOUR SGFC


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OfflineWindWisperer


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Re: My 1st Mini Tubs [Re: newrook]
    #22136938 - 08/24/15 06:47 PM (8 years, 5 months ago)

Are you using SFDs? It looks like the foil isn't sitting as high up as it would with poly. Also record how long it takes to recover from the shake, and post it back here, that could be valuable determining whether or not you need to worry about contams.

[EDIT] Also in that first jar is it wispy all over in the jar like that, or just along the glass? Popping some kernels during the prep could spread some starch around in the jars, and the myc will definitely go for it.


Edited by WindWisperer (08/24/15 06:49 PM)


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Offlinexachisonfire
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Re: My 1st Mini Tubs [Re: WindWisperer]
    #22137228 - 08/24/15 07:43 PM (8 years, 5 months ago)

Thats a very valid point. I did notice quite a few popped seeds. The starch being smeared along the glass during the shaking makes perfect sense. Thank you for that.

4 of the 7 jars have silicone injection ports AND polyfill. The other 3 have only polyfill. All jars have a layer of tyvek under the lids.

I tried to have a solid game plan before i started the whole process. I have ALOT of spare time at work, so i spend hours reading the forum. I've had changes mid stride because of what i've read. "This way is better, that way is better." My lids were one of those changes. I left the foil on for extra protection. Plus, high co2 levels promote good myc growth from what i understand so i didnt see an issue leaving the foil on.


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OfflineDarkhome
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Re: My 1st Mini Tubs [Re: xachisonfire]
    #22137251 - 08/24/15 07:48 PM (8 years, 5 months ago)

I would take the foil off:shrug:
Your filters will block contaminants... You need a little gas exchange.


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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Offlinexachisonfire
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Re: My 1st Mini Tubs [Re: Darkhome]
    #22137351 - 08/24/15 08:06 PM (8 years, 5 months ago)

Just a quick update also:

I made my 1st payste plates this evening. I've been very excited to try agar. As i said in OP, i want to use my extra 7th jar to isolate an aggressive area of my myc from said jar. That way i can reap the benefits and cut alot of time and worries in my future endeavors.

I used 2 1/2 "heaping" tablespoons of ground agar agar strips mixed with about 1 1\2 cups of grain water from my boil. I also added a few drops of honey and some green food coloring so my cultures will be easier to view through these mini round containers.


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Offlinexachisonfire
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Re: My 1st Mini Tubs [Re: Darkhome]
    #22137357 - 08/24/15 08:07 PM (8 years, 5 months ago)

The foil has been removed :cool:
Thanks!!


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OfflineDarkhome
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Re: My 1st Mini Tubs [Re: xachisonfire]
    #22137421 - 08/24/15 08:25 PM (8 years, 5 months ago)

:billymaythumbup:


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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OfflineTravelAgency
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Re: My 1st Mini Tubs [Re: Darkhome]
    #22138096 - 08/24/15 11:03 PM (8 years, 5 months ago)

:threadmonitor:


--------------------





Indoor Greenhouse Build 
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OfflineCujllickduo
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Re: My 1st Mini Tubs [Re: TravelAgency]
    #22138109 - 08/24/15 11:06 PM (8 years, 5 months ago)

Its all looking good so far:thumbup:

keep us updated i hope it all works out for you man :smile::mushroom2:


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OfflineWindWisperer


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Re: My 1st Mini Tubs [Re: Cujllickduo]
    #22141840 - 08/25/15 04:54 PM (8 years, 5 months ago)

Looking good! Let us know how the plates work out for you, it seems some myc likes high nutrition value agar, and some like a little less nutrition. I am curious to see how your plate that utilized grain water works out.


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Offlinexachisonfire
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Re: My 1st Mini Tubs [Re: WindWisperer]
    #22161362 - 08/29/15 03:46 PM (8 years, 4 months ago)







Update with a quick question:
So i stated a week ago that i thought my jars were pretty close to being ready to spawn to my tubs. I gave them a good shake and still saw quite a bit of uncolonized grains. So i let them go for another week. The myc recovered in about a day and a half.
So i looked again today to see how they were doing and they looked great. (See 1st picture.) All jars were 99% solid white.
So to make sure it wasn't just on the surface again, i shook them.
I have HUGE clups in these jars. I went after those bad boys with a rubber mallet and still couldn't break those big chunks apart. (See 2nd and 3rd pitures.)
Anyway...this is my first run with grains so im just curious if they're actually good to go? Is yhe shaking process rubbing the myc off the grains and making it look like its not fully colonized? We're approaching the 5th week since inoculation.

The Payste plates came out great! I cant wait to get some transfers going, just waiting on these jars! :wink:


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OfflineMachiavelliavore
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Re: My 1st Mini Tubs [Re: xachisonfire]
    #22161704 - 08/29/15 05:16 PM (8 years, 4 months ago)

I'm extremely suspicious of this jar


Any time I've had empty space filled with apparent "structureless milkiness" it's been contaminated.

Also, you said two 1/4" wholes on the bottom?  Should be more in the 1-1.5" diameter range, though the holes in your pic look like they are.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinexachisonfire
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Re: My 1st Mini Tubs [Re: Machiavelliavore]
    #22161743 - 08/29/15 05:32 PM (8 years, 4 months ago)

Quote:

Machiavelliavore said:
I'm extremely suspicious of this jar


Any time I've had empty space filled with apparent "structureless milkiness" it's been contaminated.

Also, you said two 1/4" wholes on the bottom?  Should be more in the 1-1.5" diameter range, though the holes in your pic look like they are.




Yeah, that 1/4" was a typo...i apologize. The drilled holes are 1 1/4" on the long sides.

That picture was taken about a week ago. And as i said, i shook them and the myc recovered just fine (it seems). I think the voided space there is because of the size of clumps in the jars. The milkyness i think may be an illusion. The myc being as whispy as it is could make it look cloudy...especially with not so great photos.
I could be wrong though...you may be right. But after looking at them a couple times through the week, they seem to be doing pretty good...


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OfflineWindWisperer


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Re: My 1st Mini Tubs [Re: xachisonfire]
    #22165405 - 08/30/15 04:22 PM (8 years, 4 months ago)

I'd say do a grain to agar transfer, so you can learn agar, clean up some plates and have more ready to go just in case those jars are contaminated. Seriously agar has allowed me to get away from MS failures.

Go ahead and buy a tote that is about as wide (or wider) than your shoulder span, it helps to get one with a clear lid. Cut some arm holes in such a way to give you good maneuverability inside your still air box. I used a dremel to cut out a circle shape, then cleaned it up with a sanding disk. You can also use a coffee can that is all metal to melt a hole with a torch.

Doing this is the best way to have another project rolling that is almost ensured success just in case! Trust me you don't want to rely on one thing, and have it fail miserably, it hurts the feels.

[EDIT] I cannot confirm whether or not your jars are contaminated, sorry man. I would pluck a grain out of the jars when you spawn them to coir verm gypsum (or substrate of choice), and plop it right in the middle of your plate. Do this with a few, and have a few plates ready to go so just in case you get contams on your plates, you have a new one ready to accept a slice of clean colonized agar.


Edited by WindWisperer (08/30/15 04:30 PM)


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