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OfflineMagicDave
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Grey Oyster Cultivation - First Attempt
    #22134712 - 08/24/15 09:43 AM (8 years, 5 months ago)

Just wanted to share my excitement about my first attempt with cultivating mushrooms. Since this is my first attempt, I am going with a low tech solution. I am colonizing jars, 4 with BRF/verm, and 3 with organic rye berries/verm. I am at the point where I am waiting for colonization, they have only been sitting for a day now. Any tips?



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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22134726 - 08/24/15 09:47 AM (8 years, 5 months ago)

Nice shorts!:cookiemonster:


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OfflineMagicDave
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Re: Grey Oyster Cultivation - First Attempt [Re: mcchieftan]
    #22135123 - 08/24/15 11:40 AM (8 years, 5 months ago)

Quote:

mcchieftan said:
Nice shorts!:cookiemonster:




Lol. Did this late at night, they are my sleepy time shorts.


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OfflineMagicDave
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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22143003 - 08/25/15 08:29 PM (8 years, 5 months ago)

I have a question, about bags that I see everyone growing mushrooms in.

What kind of bags to people use? Do you just put your substrate in it, put some spawn in it, and cut holes in the bag so that the mushrooms can pin out?


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22143267 - 08/25/15 09:21 PM (8 years, 5 months ago)

Typically we grow oysters on some sort of wood based substrate. Get some hardwood mulch/chips, wood pellets, cardboard, or sawdust and pasteurize the substrate then mix with your spawn and fill the bags. Typically WBS, milo, wheat, or rye is used for spawn since it breaks up easier into even sized balls for inoculation points of you bulk substrate. Also you can do GTG and continue your culture easier with grain spawn. You can use anything including 20lb ice bags, gallon ziplocks, bread loaf bag, shopping bags, purpose built XL mushroom grow bags or poly tube with the ends tied or impulse sealed. I use grow bags and 5 gal pails currently and im using WBS or milo spawn made in quart jars and bulk pasteurized mixed mulch using the hot water bath method.


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OfflineMagicDave
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Re: Grey Oyster Cultivation - First Attempt [Re: Gr0wer]
    #22143369 - 08/25/15 09:47 PM (8 years, 5 months ago)

Do you cut holes in the bags for the mushrooms to fruit out of? How big of holes would you recommend, for an ice bag for instance, and how many?


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OfflineGr0wer
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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22143764 - 08/25/15 11:10 PM (8 years, 5 months ago)

I cut my holes every 4-6" and make 1" X's, straight slices or 1/2" triangle cutouts. It depends on what mood im in, they all work just as well. Cut em once there stuffed with a razor or scalpel, some use 4 blade hunting arrows to make quick X's. In my pails i do 1/4-1/2" holes every 5". The holes should be on the sides, any pins on the top typically abort when there are side fruits.


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OfflineMagicDave
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Re: Grey Oyster Cultivation - First Attempt [Re: Gr0wer]
    #22144695 - 08/26/15 07:17 AM (8 years, 5 months ago)

Thanks, very helpful. How much spawn do you typically use? Is there a ratio or formula of spawn to substrate weight?


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22144915 - 08/26/15 08:16 AM (8 years, 5 months ago)

Very organized! funny to someone cultivate them though because they have been growing like crazy in the wild where I live. too bad i dont really care for them :p


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OfflineMagicDave
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Re: Grey Oyster Cultivation - First Attempt [Re: Helvella]
    #22148866 - 08/26/15 08:47 PM (8 years, 5 months ago)

Three days in and I already have visible mycelium in my jars. that has to be a good sign right?!


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22148933 - 08/26/15 08:57 PM (8 years, 5 months ago)

Sounds good! oysters typically are wispy and thin then will turn solid white a few days later. So dont be alarmed when it almost looks like cobweb.


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Re: Grey Oyster Cultivation - First Attempt [Re: Gr0wer]
    #22148972 - 08/26/15 09:03 PM (8 years, 5 months ago)

Do you have spare culture? If not definitely plan on transferring some of the growth to a few jars of grain spawn to continue the culture. I just saw now you made jars of rye/verm, that would be best to use for GTG and ditch the verm to use it as spawn for bulk sub.


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Re: Grey Oyster Cultivation - First Attempt [Re: Gr0wer]
    #22149067 - 08/26/15 09:20 PM (8 years, 5 months ago)

What is GTG? I looked here http://www.shroomery.org/forums/showflat.php/Number/14956808#14956808 and didn't see it.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22151231 - 08/27/15 12:19 PM (8 years, 5 months ago)

Will the rye berries not contam?  He's using a pot on the stove with a lid.  Not a pc


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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22151469 - 08/27/15 01:18 PM (8 years, 5 months ago)

I steam sterilize my grains for 4 hours and still not one to contaminate


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OfflineMagicDave
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Re: Grey Oyster Cultivation - First Attempt [Re: nooberst]
    #22151722 - 08/27/15 02:23 PM (8 years, 5 months ago)

That would be a bummer, but if it happens it happens I guess. Will the BRF cakes be okay? I read several things that said a pressure cooker wasn't necessary as long as you give them enough time in a pot.


Edited by MagicDave (08/27/15 02:27 PM)


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OfflineMagicDave
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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22154131 - 08/27/15 10:14 PM (8 years, 5 months ago)

Here is 5 days after noc'ing them. The right 4 are my Rye spawn, the left 3 are BRF cakes. As you can see, there is barely any mycelium on the BRF, but the rye is covered in mycelium. Is this normal?



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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22155407 - 08/28/15 08:51 AM (8 years, 5 months ago)

How long did you steam em for?  I think the brf will be fine by steaming but the rye I dunno man.  people PC the rye at 15psi for atleast an hour that's 250 degrees(I just ordered parts so I can modify my PC into a sterilizer and do about 20psi for an hour)...... some people longer.

When I started (and trust me I'm still a newbie learning) I had a small electric pc I used.  I think I could fit 6 pints in there.  Wasn't long before I found an $80 all American 915 on ebay and shit is still too small for me I think but it's an improvement.


Edited by midnightmaraude (08/28/15 08:53 AM)


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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22155420 - 08/28/15 08:55 AM (8 years, 5 months ago)

Also, it doesn't look like you have any micropore tape over your holes.  Not super important because of the verm layer on top but I don't like to take chances.


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OfflineMagicDave
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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22156345 - 08/28/15 12:11 PM (8 years, 5 months ago)

Quote:

midnightmaraude said:
Also, it doesn't look like you have any micropore tape over your holes.  Not super important because of the verm layer on top but I don't like to take chances.




I read enough articles that convinced me that the vermiculite was enough and decided to leave them that way.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22156356 - 08/28/15 12:13 PM (8 years, 5 months ago)

I steamed them for an hour and a half. I don't know at what temp. Just kept the water boiling for the entire hour and a half.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22167883 - 08/31/15 06:51 AM (8 years, 4 months ago)

keep me posted on the rye jars please.  I'm curious if they are going to contam or not.  They may even finish growing out but when you put them to fruit I'm certain a contam will show... trich etc.  If it does turn green get rid of it quick and safely.


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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22168145 - 08/31/15 08:50 AM (8 years, 4 months ago)



Everything looks fine so far. I will keep this thread updated.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22168174 - 08/31/15 09:03 AM (8 years, 4 months ago)

Last post was actually the BRF cakes. This one shows all, the 3 on the left are the Rye, and the 4 on the right are the BRB cakes:



Most likely you cannot see any mycelium on the rye in the pics. If I look close I can see a little mycelium. Does this show a problem, or does rye just colonize slower than rice flour?


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22169707 - 08/31/15 03:29 PM (8 years, 4 months ago)

I am personally thinking the BRF cakes are most likely going to be fine, and that I am screwed with the Rye. I am most likely going to be buying a pressure cooker, so I do have a question.

I don't want to have to order more spores, so if I am able to get Oyster mushrooms from the BRB cakes, will it be a fairly easy process to create spawn from the mushrooms growing from the BRF?


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22169837 - 08/31/15 04:05 PM (8 years, 4 months ago)

Following.
Interested in your progress.
I recently acquired some Choc Oyster spawn and am interested in reading along.
Thank you for your work.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22177722 - 09/02/15 11:04 AM (8 years, 4 months ago)

Quote:

MagicDave said:
I am personally thinking the BRF cakes are most likely going to be fine, and that I am screwed with the Rye. I am most likely going to be buying a pressure cooker, so I do have a question.

I don't want to have to order more spores, so if I am able to get Oyster mushrooms from the BRB cakes, will it be a fairly easy process to create spawn from the mushrooms growing from the BRF?




Well you'll have to get into doing agar work if you want to make more oyster myc.  Once you grow an oyster, there is a tek you can follow to scrape some of the tissue from the inside of the stem and place onto agar so you clone that mushroom and have a big plate of myc you can use.

I don't know much about oysters (I have a vile of blue oyster myc I will be putting to use soon) but if they drop spores you could also spread some spores on agar and let it grow out that way too although it will be MS and not a clone.


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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22218291 - 09/10/15 07:09 PM (8 years, 4 months ago)

Just go give a quick update...

I birthed by BRF cakes today. I decided to do the dunk and roll method, so they are currently soaking.

Also the Rye that was not colonizing actually started to colonize. I know they will probably still contaminate, but I am going to let them go for a while. They are probably only 15% colonized.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22220639 - 09/11/15 08:03 AM (8 years, 4 months ago)

often times they won't colonize if there is bacteria.  I would be very careful.  If you see green it may be too late and you'll have trich spores all over your house.
Think about it.

Rye is a great medium and myc would have colonized it already


Edited by midnightmaraude (09/11/15 08:04 AM)


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OfflineMagicDave
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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22220825 - 09/11/15 08:54 AM (8 years, 4 months ago)

Quote:

midnightmaraude said:
often times they won't colonize if there is bacteria.  I would be very careful.  If you see green it may be too late and you'll have trich spores all over your house.
Think about it.

Rye is a great medium and myc would have colonized it already




Thanks midnightmaraude. I may dispose of them today.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22220837 - 09/11/15 08:56 AM (8 years, 4 months ago)

At this point, I have dunked the BRF cakes for 24 hours, and rolled them in verm. I placed them in my terrarium with perlite that I sprayed with water to keep the humidity up.



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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22245950 - 09/16/15 12:54 PM (8 years, 4 months ago)

Update:



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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22246051 - 09/16/15 01:19 PM (8 years, 4 months ago)

Shit man that looks cool.  I can't tell from the pics but are they sitting right on the perlite?  If so you should really elevate them or they will dry out from the perlite absorbing the moisture in the cake.

Put them on a lid, or something.


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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22246054 - 09/16/15 01:20 PM (8 years, 4 months ago)

could stand to raise it up a bit higher too from the floor.


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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22246324 - 09/16/15 02:28 PM (8 years, 4 months ago)

Quote:

midnightmaraude said:
Shit man that looks cool.  I can't tell from the pics but are they sitting right on the perlite?  If so you should really elevate them or they will dry out from the perlite absorbing the moisture in the cake.

Put them on a lid, or something.




Yeah, they are sitting right on the perlite. I soaked the perlite in water beforehand also. I used the instructions from these videos: http://www.shroomery.org/11432/Lets-Grow-Mushrooms-Pf-tek-videos

It is pretty exciting for me, this is my first attempt at growing anything ever. I am currently colonizing substrate with some other spores that I ordered also. :mushroom2:


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22246326 - 09/16/15 02:29 PM (8 years, 4 months ago)

Oh yeah, and I actually have it raised higher up, it is not directly on the floor like in one of the previous pictures.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22249865 - 09/17/15 08:04 AM (8 years, 4 months ago)

Quote:

MagicDave said:
Quote:

midnightmaraude said:
Shit man that looks cool.  I can't tell from the pics but are they sitting right on the perlite?  If so you should really elevate them or they will dry out from the perlite absorbing the moisture in the cake.

Put them on a lid, or something.




Yeah, they are sitting right on the perlite. I soaked the perlite in water beforehand also. I used the instructions from these videos: http://www.shroomery.org/11432/Lets-Grow-Mushrooms-Pf-tek-videos

It is pretty exciting for me, this is my first attempt at growing anything ever. I am currently colonizing substrate with some other spores that I ordered also. :mushroom2:



:ohnowaitokay:


http://www.shroomery.org/forums/showflat.php/Number/798467#798467


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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22268859 - 09/21/15 11:02 AM (8 years, 4 months ago)

I'm not a Trusted Cultivator,
but I feel like even in my noobie
lack of wisdom I may still impart the knowledge of...

Not putting your substrate directly onto the perlite.
Use foil, a tray, a rack, even your jar lid - which is what most people do, I gather.


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Re: Grey Oyster Cultivation - First Attempt [Re: mwhtmn]
    #22270249 - 09/21/15 04:18 PM (8 years, 4 months ago)

Thanks for the tips everyone about the mushrooms not being in the perlite. I fixed the issue.

Does anyone know what the general rule is for when to harvest Oysters? Do a wait until they get a certain size?


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22270788 - 09/21/15 06:24 PM (8 years, 4 months ago)

Quote:

MagicDave said:
Thanks for the tips everyone about the mushrooms not being in the perlite. I fixed the issue.

Does anyone know what the general rule is for when to harvest Oysters? Do a wait until they get a certain size?




Just before the edges curl up. For most anyway. Pinks are strange, they always seem to look like they are curling up. The big thing is, before the spores begin to drop too much. With pinks at the first sign of any spores I pick, or even before.


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Edited by Jim Morrison (09/21/15 06:26 PM)


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Re: Grey Oyster Cultivation - First Attempt [Re: Jim Morrison]
    #22274196 - 09/22/15 01:48 PM (8 years, 4 months ago)

Thanks. Dang, they started dropping spores today and are not that big.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22274864 - 09/22/15 04:08 PM (8 years, 4 months ago)

Pics man


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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22277776 - 09/23/15 08:18 AM (8 years, 4 months ago)



The first pictures are the mushrooms that were dropping their white spores on the foil that I had under them. The second pictures is the rest of my BRF cakes with the others.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22277906 - 09/23/15 09:11 AM (8 years, 4 months ago)

oh gawd.  Those do not look healthy at all man.  I don't even know.  Try again man.  It looks like mold grew on the cakes too, possibly from soaking up the water from the perelite. Don't let the experience discourage you. 


Edited by midnightmaraude (09/23/15 09:13 AM)


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22277909 - 09/23/15 09:13 AM (8 years, 4 months ago)

Quote:

MagicDave said:


The first pictures are the mushrooms that were dropping their white spores on the foil that I had under them. The second pictures is the rest of my BRF cakes with the others.




Whoa! Those need a lot more air.


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Re: Grey Oyster Cultivation - First Attempt [Re: Jim Morrison]
    #22277937 - 09/23/15 09:21 AM (8 years, 4 months ago)

Were you fanning at all along with the misting?


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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22277968 - 09/23/15 09:32 AM (8 years, 4 months ago)

Like Midnight said; don't be discouraged. Starting out (still am), I grew allot of mold. And my shrooms didn't look so hot.


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Re: Grey Oyster Cultivation - First Attempt [Re: Jim Morrison]
    #22279170 - 09/23/15 02:48 PM (8 years, 4 months ago)

I havn't been fanning a lot after misting. Is it best to put an actual fan on them?


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22279220 - 09/23/15 03:01 PM (8 years, 4 months ago)

I don't have enough experience to offer exact solutions, but a direct fan would dry them out too much. You would need some type of humidifier in there too.


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Live Long and Myceliate!

The only power any government has is the power to crack down on criminals. Well, when there aren't enough criminals, one makes them. One declares so many things to be a crime that it becomes impossible for men to live without breaking laws. Ayn Rand


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Re: Grey Oyster Cultivation - First Attempt [Re: Jim Morrison]
    #22282948 - 09/24/15 09:54 AM (8 years, 4 months ago)

Just mist a couple times a day, then I take the lid and fan em with it.
I'm not an expert at all I'm just wondering what that white fuzz is.. is that a result of low fae or some sort of mold.  Probably lack of air but maybe post your pic over on the contamination forum and ask them what they think.


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Re: Grey Oyster Cultivation - First Attempt [Re: midnightmaraude]
    #22303074 - 09/28/15 02:09 PM (8 years, 4 months ago)

I too am no expert but, I agree, that fanning with the lid helps reduce the long thin stems by reducing CO2.  I'd say 1-3 times per day and you want to get the air moving a bit, you're trying to get the CO2 out which is a bit heavier than the other ambient gases... I think.  Attaching a computer vent fan with a power plug on a timer might be the easier way.

If you have a humidity gauge in there, what does the humidity top out at?  If it's a bit low I'd consider taping over some of the vent holes in your humidity box.

Again if I were you and was looking to clone the oyster mushroom fruit-bodies or cakes I currently had growing and wanted something simpler than agar I would want know to about cloning on pasteurized cardboard.  It is easy and low contaminate probability.  I have had no problems putting the butts from store bought oyster mushrooms on to pasteurized cardboard in freezer bags for cloning.  No need for center cutting, I have torn up whole stems with my (cleaned) bare hands.  From there go to a slightly cleaner style transfer to PCed grain.

(Oyster stem pieces just starting on cardboard)




If we are all thinking the white fuzzy at the bottom of the cakes where it meets the perlite that's stuck to the cake is mold I would like to counter-postulate that it may just be mycelial regrowth from being set on wet perlite in high CO2.  I'm no expert though.

If I where you I would harvest what's there if you haven't yet.  Dunk the cakes.  Put them back in there on their foil, make sure they don't sit on the foil in leftover dunk water after being set there for a bit.  I would work on ventilation more for next flush.  And I'd think about checking my humidity %.  Also, congratulations on growing some mushrooms! :smile:


Edited by vvHeavyvv (09/28/15 02:14 PM)


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Re: Grey Oyster Cultivation - First Attempt [Re: vvHeavyvv]
    #22304614 - 09/28/15 07:05 PM (8 years, 4 months ago)

Oysters need tons of FAE. Look into rigging up a cool mist to push in fresh air or a small computer fan with an ultramist on a timer or humidistat.  Also vent your exhaust out a nearby window or vent if possible especially around harvest time due to spores. If you want to take the easier route you can try cracking the lid and misting/fanning often. Or if your conditions are right outside just fruit in a shady location.


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Re: Grey Oyster Cultivation - First Attempt [Re: Gr0wer]
    #22305001 - 09/28/15 08:27 PM (8 years, 4 months ago)

:whathesaid:

Still though, if he fixes the FAE these cakes should give him another flush correct?

I think he should keep trying to fix the problem with these that way next run will be tits!
:jiggly:

It's not over yet!
I don't think theres mold there, but i could be wrong..


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Re: Grey Oyster Cultivation - First Attempt [Re: confuzzed]
    #22307206 - 09/29/15 09:22 AM (8 years, 4 months ago)

When at least expected it yesterday, I got some decent mushrooms actually. So that was great. I'll definitely keep trying also.


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Re: Grey Oyster Cultivation - First Attempt [Re: MagicDave]
    #22307549 - 09/29/15 10:59 AM (8 years, 4 months ago)

"Pics man" :smile:


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