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Indigo-Child
Stranger
Registered: 10/04/10
Posts: 40
Last seen: 7 years, 5 months
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No germination after 6 days (old spores)
#22133803 - 08/24/15 03:22 AM (8 years, 5 months ago) |
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So I'm a pretty experiened grower, have been growing on and off for the past 12 years. My last "on", I got into some exotics and really stocked my spore (syringes, no prints) collection up. This was back in 2013. I started a new grow using these syringes on rye (berries) a few days back (the 18th), 6 jars (2 pan cyan, 1 galindoi, 2 burma cubensis, 1 mex jalisco). Six days later, I am only seeing germination in the galindoi and that only occured yesterday.
I've grown from old syringes before and never had an issue. The spores were kept in my little fridge (not too cold) in double ziplocks, but I am used to seeing germination within 3 days and I am starting to get nervous.
If I remember correctly I had issues getting both the pans and mex to germinate back when I got the syringes (sporeworks), but I have prints in my collection of the Burma so I know I didn't have an issue with them. For some reason I could never get proper germination off many of my exotics, I noc'd up a plate with azures as well and alas no germination there either (then again I think this batch of agar was improperly prep'd as I could never get spore germination off this batch of plates which has also been in my fridge for several years).
Am I getting nervous for no reason? It seems like I should be seeing something by now. It just would be such a damn shame if all of these beautiful exotic spores went to waste before I was able to collect some prints. The only exotic prints I managed to take are of the galindoi (I have WELL over a 100 prints collected...), of course they are the one species that is germinating fine from the syringe.
For the record I prepared the rye with a coffee solution (perhaps a bit stronger than should be...), I also added a micro spatula of Ca(OH)2 to the grain before simmering as I didn't have any gypsum on hand. I also noticed my incubator feels far warmer than it should be (and so I just now turned off the heat to see what happens), but I can't imagine that would upset germination as much as it would slow growth.
Any opinions?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 21 hours
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Re: No germination after 6 days (old spores) [Re: Indigo-Child]
#22133825 - 08/24/15 03:39 AM (8 years, 5 months ago) |
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Spore germination can take 2+ weeks in some cases.
Incubate nothing....room temp is fine.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: No germination after 6 days (old spores) [Re: PussyFart]
#22133841 - 08/24/15 03:48 AM (8 years, 5 months ago) |
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Patience is key. Spores will germinate at random rates. Ive literally had one take a little over a month before it started showing it's first signs of growth.
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: No germination after 6 days (old spores) [Re: mushmagic]
#22133998 - 08/24/15 05:40 AM (8 years, 5 months ago) |
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You should remove the vendors name from the OP.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
Edited by Buck513 (08/24/15 05:46 AM)
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Indigo-Child
Stranger
Registered: 10/04/10
Posts: 40
Last seen: 7 years, 5 months
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Re: No germination after 6 days (old spores) [Re: Buck513]
#22136364 - 08/24/15 04:43 PM (8 years, 5 months ago) |
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So not to worry yet then? OK thanks guys! I did kill the heat as well so we'll see what happens.
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