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Offlinenewera
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Just going to start here....
    #22132268 - 08/23/15 06:28 PM (8 years, 5 months ago)

I'm at a loss right now trying to get a good grow happening!
So I'm going to post everything i do here with pics along the way, hope to get some good help!going to start some no pour agar right now via professor pinheads tek:tipofthecap:


okay so here it is i have some jars for no pours(lids are to be drilled in minute, well just a screw driver and then stuffed tight with poly) and i have premixed mea i ordered online and a jar to mix it in, should i be mixing it and running it through the pc first...seems easy enough to mix and put through a quick 15 minute cycle this should dissolve evrything thing, then i plan to put enough for a flat surface on the bottom and put the lids on tight, cover with foil and pc at 15 psi for 20-30 minutes


Edited by newera (08/23/15 09:05 PM)


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OfflinePsilosoulful

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Re: Just going to start here.... [Re: newera]
    #22132493 - 08/23/15 07:19 PM (8 years, 5 months ago)

:goodluck:


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OfflineOPB
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Re: Just going to start here.... [Re: Psilosoulful]
    #22132574 - 08/23/15 07:48 PM (8 years, 5 months ago)

Maybe its just me but this seems like a terrible way to get help. One thread with a bunch of questions requires someone to read through everything to see where you are at and see what recent questions you have had. Well thought out individual threads and much easier to supply answers too and also result in more searchable valuable content for other members.

I could be crazy for thinking this but it seems logical to me. Good luck either way.


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InvisibleMudaFuka
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Re: Just going to start here.... [Re: OPB]
    #22132600 - 08/23/15 07:56 PM (8 years, 5 months ago)

Doing it this way is fine as long as the OP is updated regularly.


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Offlinenewera
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Re: Just going to start here.... [Re: OPB]
    #22132605 - 08/23/15 07:58 PM (8 years, 5 months ago)

Hmmm interesting point, I'm not new to growing by any means, i've done mostly everything from agar to strain isolation I've just been running into the odd problem lately, so you think I'm better off just posting questions when I have them? I thought  a grow log would be a good idea but I could be greatly mistaken here I guess,  just figured I'd keep my original post updated and go from there:shrug:


Edited by newera (08/23/15 07:59 PM)


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Offlinenewera
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Re: Just going to start here.... [Re: newera]
    #22132875 - 08/23/15 09:01 PM (8 years, 5 months ago)


okay so here it is i have some jars for no pours(lids are to be drilled in minute, well just a screw driver and then stuffed tight with poly) and i have premixed mea i ordered online and a jar to mix it in, should i be mixing it and running it through the pc first...seems easy enough to mix and put through a quick 15 minute cycle this should dissolve evrything thing, then i plan to put enough for a flat surface on the bottom and put the lids on tight, cover with foil and pc at 15 psi for 20-30 minutes


Edited by newera (08/23/15 09:04 PM)


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InvisibleMudaFuka
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Re: Just going to start here.... [Re: newera]
    #22133048 - 08/23/15 09:54 PM (8 years, 5 months ago)

You don't need to PC twice just mix your agar into Boling water, pour then PC.


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Offlinenewera
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Re: Just going to start here.... [Re: MudaFuka]
    #22133785 - 08/24/15 03:07 AM (8 years, 5 months ago)

Quote:

MudaFuka said:
You don't need to PC twice just mix your agar into Boling water, pour then PC.



I just tried this and the second i dumped the agar into the boiling hot water i just got huge chunks of agar floating around no matter how much i stir it...what now i can't use this and it's been 15 minutes and nothing has dissolved...still huge chunks in there lol
i can't even get the premixed shit right:facepalm3:


Edited by newera (08/24/15 03:12 AM)


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Offlinenewera
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Re: Just going to start here.... [Re: newera]
    #22133853 - 08/24/15 03:53 AM (8 years, 5 months ago)

This stuff shoudl have a dissolving temp shoudl it not? I've had this simmering for almost 20 minutes and these chunks will not go away:shrug:

edit:30 minutes of simmering and trying to mix it up i got nothing, fuck me i'm over before i even started


Edited by newera (08/24/15 04:01 AM)


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Offlinechrismcq
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Re: Just going to start here.... [Re: newera]
    #22133892 - 08/24/15 04:19 AM (8 years, 5 months ago)

Agar melts at 85 °C ( 185 °F) and solidifys at 32–40 °C (90–104 °F) so chunks should be gone by now :sad:
I like to use Agar Agar Powder, It dissolves much easier than a block. Maybe try again and cut the agar in to small pieces before devolving..
if this fails some agar agar powder is worth getting.


Edited by chrismcq (08/24/15 04:20 AM)


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InvisibleMudaFuka
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Re: Just going to start here.... [Re: chrismcq]
    #22134612 - 08/24/15 09:15 AM (8 years, 5 months ago)

Some agar needs to be mixed into cold water and be brought up to a bile. Sorry. I didn't mention that.


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Offlinenewera
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Re: Just going to start here.... [Re: MudaFuka]
    #22135475 - 08/24/15 01:24 PM (8 years, 5 months ago)

Quote:

MudaFuka said:
Some agar needs to be mixed into cold water and be brought up to a bile. Sorry. I didn't mention that.



:facepalm3:dammit, well i guess i'll try another time i don't have any left and that shit was expensive, no Asian markets around here either, oh well maybe i'll just order a fucking grow kit


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InvisibleMudaFuka
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Re: Just going to start here.... [Re: newera]
    #22135710 - 08/24/15 02:26 PM (8 years, 5 months ago)

I get most of my agar from a heath food store.


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InvisibleJuiceh
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Re: Just going to start here.... [Re: MudaFuka]
    #22135898 - 08/24/15 02:58 PM (8 years, 5 months ago)

When adding your agar mixture to the water you want to add it slowly while stirring constantly. If you add the powdered ingredients too quickly you will get bad clumping problems that are pretty damn hard to fix. I do not recommend adding the agar to boiling water, hot water a little below boiling on the stove works better for me. Boiling water tends to get more of the non dissolved mixture thrown up against the sides of the pot above the water level where it can burn.


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Offlinenewera
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Re: Just going to start here.... [Re: Juiceh]
    #22138567 - 08/25/15 02:47 AM (8 years, 5 months ago)

Well I don't' know what I can do now, try salvage what I can from this agar I suppose, one thing after another with me and this hobby though:facepalm3:
I have some penis envy swabs which are about 2 years old but i can't find a tek saying how swabs are used,  do you just streak plates like you would with a loop or cut the tip off?


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InvisibleJuiceh
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Re: Just going to start here.... [Re: newera]
    #22139661 - 08/25/15 08:06 AM (8 years, 5 months ago)

Streak plates with the swab.


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Offlinenewera
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Re: Just going to start here.... [Re: Juiceh]
    #22141803 - 08/25/15 04:47 PM (8 years, 5 months ago)

Quote:

Juiceh said:
Streak plates with the swab.



Cool thanks, i simmered the agar again and ran it through a strainer, now i'm worried i threw off the nute content and the agar content, i can handle soft agar but any body know of any othter problems that may pop up? should i bother with it or just say fuck it and order a vendor syringe?


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OfflinecronicrFacebook
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Re: Just going to start here.... [Re: newera]
    #22142104 - 08/25/15 05:35 PM (8 years, 5 months ago)

add a couple drops of karo or even brf if you have any, i wouldn't' worry about it myself


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Offlinenewera
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Re: Just going to start here.... [Re: cronicr]
    #22148801 - 08/26/15 08:35 PM (8 years, 5 months ago)


after re-simmering and straining the chunks out i was left with this:shrug: looks fine and it solidified okay...think i should use it?


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InvisibleMad Season
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Re: Just going to start here.... [Re: newera]
    #22149612 - 08/27/15 12:20 AM (8 years, 5 months ago)

Yes.


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InvisibleBig Bear
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Re: Just going to start here.... [Re: newera]
    #22149774 - 08/27/15 02:09 AM (8 years, 5 months ago)

if it's premixed MEA (malt extract agar) a wisk helps tremendously.  I usually get it to a simmer and dump half in and wisk it around until it disolves, then dump the other half in and do the same thing.  MEA can be kind of tricky to get to dissolve IME


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Offlinenewera
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Re: Just going to start here.... [Re: Big Bear]
    #22157401 - 08/28/15 04:21 PM (8 years, 5 months ago)

well got some plates inoculated, here's to hope:waits:
used all my spores on this shit


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Offlinenewera
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Re: Just going to start here.... [Re: newera]
    #22157402 - 08/28/15 04:22 PM (8 years, 5 months ago)

any edibles i could try this tek with?


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InvisibleJuiceh
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Re: Just going to start here.... [Re: newera]
    #22158093 - 08/28/15 07:23 PM (8 years, 5 months ago)

Quote:

newera said:
any edibles i could try this tek with?



All of them.


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Offlinenewera
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Re: Just going to start here.... [Re: Juiceh]
    #22186072 - 09/04/15 12:40 AM (8 years, 4 months ago)

1 week later...zero growth from pe swabs zero growth from cambo prints zero growth from pan prints other then some bacteria:sad:


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InvisibleJuiceh
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Re: Just going to start here.... [Re: newera]
    #22187480 - 09/04/15 11:09 AM (8 years, 4 months ago)

Spores can take quite some time to germinate. 1 Week isn't much.


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Offlinenewera
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Re: Just going to start here.... [Re: Juiceh]
    #22187541 - 09/04/15 11:22 AM (8 years, 4 months ago)

i'mm seeing satellite colonies now though from my pe swab plates, should i just toss those? that leaves me down to my cambodians?
starting to think agar just isn't' for me lol, i never had issues when i used spore syringes...i've had luck putting fruits to agar but not once in a year have i had any good success starting from spores on agar, rarely get anything to germinate...maybe i should switch to petris:shrug:


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OfflinecronicrFacebook
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Re: Just going to start here.... [Re: newera]
    #22187653 - 09/04/15 11:45 AM (8 years, 4 months ago)

just wait....stop freaking out already, it's a numbers game so start lots and go from there


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Offlinenewera
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Re: Just going to start here.... [Re: cronicr]
    #22191207 - 09/05/15 01:26 AM (8 years, 4 months ago)

You are right i was talking mad again, something i do.....when do you throw in the towel though right?


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InvisibleJuiceh
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Re: Just going to start here.... [Re: newera]
    #22191231 - 09/05/15 01:39 AM (8 years, 4 months ago)

You throw in the towel when the plate contams, or dries out.


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Offlinenewera
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Re: Just going to start here.... [Re: Juiceh]
    #22191235 - 09/05/15 01:42 AM (8 years, 4 months ago)

well my plates are contaminating, I could have worded that better but I'm still asking what you should do when you get sporulating mold before your spores germinate?


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Offlinenewera
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Re: Just going to start here.... [Re: newera]
    #22191252 - 09/05/15 01:53 AM (8 years, 4 months ago)

ok well now i see my cambo plates showing what i'm guessing to be mold where they were swiped which leaves me with contaminated PE plates, nothing left after this so here's to fucking hope!!!!!!!!!!!!!


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