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InvisibleAureus
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Registered: 07/04/15
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Combining multispore cakes when making bulks
    #22132041 - 08/23/15 05:40 PM (8 years, 5 months ago)

Should I cut them into big pieces to minimize incompatible substrains?


Edited by Aureus (08/23/15 05:40 PM)


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OfflinecronicrFacebook
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Re: Combining multispore cakes when making bulks [Re: Aureus]
    #22132052 - 08/23/15 05:41 PM (8 years, 5 months ago)

they are all cubes


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Offlinespacechildo
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Re: Combining multispore cakes when making bulks [Re: Aureus]
    #22132058 - 08/23/15 05:42 PM (8 years, 5 months ago)

no, that doesnt even make sense...:confused2:

cheese grate them all into a mini mono. the myc will know what to do! :cool::thumbup:


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InvisibleAureus
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Re: Combining multispore cakes when making bulks [Re: spacechildo]
    #22132159 - 08/23/15 06:03 PM (8 years, 5 months ago)

If you cut the cake into several little pieces and mix them, you are increasing the chances of combining pieces that are not compatible.
I've also heard that the bigger the pieces the bigger the mushrooms are.




Edited by Aureus (08/24/15 03:38 PM)


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OfflinecronicrFacebook
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Re: Combining multispore cakes when making bulks [Re: Aureus]
    #22132175 - 08/23/15 06:06 PM (8 years, 5 months ago)

i don't know where you are reading this but don't read there anymore


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleAureus
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Re: Combining multispore cakes when making bulks [Re: cronicr]
    #22132273 - 08/23/15 06:28 PM (8 years, 5 months ago)

That's based in my own experiences.  I've always used big pieces and my shrooms have always been big. But last time i decided to blend the cakes to see what happens. The result is that they were all small (10cm tops) I only had 2 shrooms that were everage size.


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OfflineMajickMuffin
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Re: Combining multispore cakes when making bulks [Re: Aureus]
    #22132285 - 08/23/15 06:31 PM (8 years, 5 months ago)

Quote:

Aureus said:
That's based in my own experiences.  I've always used big pieces and my shrooms have always been big. But last time i decided to blend the cakes to see what happens. The result is that they were all small (10cm tops) I only had 2 shrooms that were everage size.



ANY kind of CUBENSIS (ANY SUBSTRAIN OF CUBENSIS) are compatible.

If you deny that right there, then just get the fuck outa here


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Offlinenewera
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Re: Combining multispore cakes when making bulks [Re: Aureus]
    #22132295 - 08/23/15 06:33 PM (8 years, 5 months ago)

naw mushrooms size is based off genetics and what moisture they can pull from the substrate and what food they have available to them, if ya get lots of pins on your first flush you are going to get small fruits which is why you get bigger fruits in later flushes and as cron said those names given by vendors are just names, you could pick a ms fruit off your cake and call it dildo lovers cubes and you wouldn't be wrong


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InvisibleAureus
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Re: Combining multispore cakes when making bulks [Re: newera]
    #22132355 - 08/23/15 06:45 PM (8 years, 5 months ago)

So there isn't such a thing as incompatible substrains?


That's all very contradicting, I've always heard Roger Rabbit and other advanced members talking about incompatible substrains and how you should not make dark syringes.


Edited by Aureus (08/23/15 06:47 PM)


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Offlinebluegill
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Re: Combining multispore cakes when making bulks [Re: Aureus]
    #22132379 - 08/23/15 06:49 PM (8 years, 5 months ago)

I found the notes you are talking about. RR was referring to sectoring on agar plates. Mycelium sectoring is not well understood but if there were such things as incompatible substrains, then spawning bulk with MS would not work as well.

Quote:

Sectoring and abnormalities in cultures

Sectoring is any type of mycelial growth that differs in appearance, growth rate and color
from the typical appearance of a given strain. Sectoring is often seen as a rapidly
growing area near the leading edge of growth in an agar slant, and often the sector will
exhibit a different growth habit than the rest of the culture. A sector may or may not
revert to normal growth. Other abnormalities that might appear in a culture are fluffy,
aerial mycelium or a color change such as browning or darkening of the mycelium.

The causes of sectoring are not well understood. It is believed that sectoring represents
some type of genetic change, perhaps loss of heterozygosity at specific loci,
deheterokaryotization, somatic recombination, or chromosomal loss in the culture. At the
present time, no method is available to determine the ultimate productivity of a culture on
agar.

Cultures are propagated solely on the basis of cultural characteristics exhibited in an agar
slant. These characteristics vary considerably from strain to strain, but experience has
shown that transfers from typical, uniform cultures of a given strain will produce reliable
spawn when handled properly. On the other hand, a sector or change in vegetative
growth could affect the productivity of the culture to an unknown extent. Therefore, it is
very important to recognize and avoid propagation of sectored mycelium.




Source: Care and handling of cultures of the cultivated mushroom (Agaricus bisporus)


--------------------
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Edited by bluegill (08/23/15 06:56 PM)


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Offlinespacechildo
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Re: Combining multispore cakes when making bulks [Re: cronicr]
    #22132381 - 08/23/15 06:50 PM (8 years, 5 months ago)

all myc is created by compatible strains, if they werent compatible they wouldnt mate and create myc.
I think you're maybe misunderstanding the quotes you read..

also the big cake chunks to bulk leads to big fruits is just whack, if that were true we'd all be using the biggest grain kernels out there.


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OfflinecronicrFacebook
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Re: Combining multispore cakes when making bulks [Re: spacechildo]
    #22132390 - 08/23/15 06:52 PM (8 years, 5 months ago)

Quote:

Aureus said:
So there isn't such a thing as incompatible substrains?


That's all very contradicting, I've always heard Roger Rabbit and other advanced members talking about incompatible substrains and how you should not make dark syringes.



Quote:

spacechildo said:
all myc is created by compatible strains, if they werent compatible they wouldnt mate and create myc.
I think you're maybe misunderstanding the quotes you read..

also the big cake chunks to bulk leads to big fruits is just whack, if that were true we'd all be using the biggest grain kernels out there.



:whathesaid: don't dig into it too much, what they mean by strains differs from what you are thinking


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleAureus
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Re: Combining multispore cakes when making bulks [Re: bluegill]
    #22132444 - 08/23/15 07:05 PM (8 years, 5 months ago)

These are the quotes I'm talking about.





****

Good luck getting those to fruit.  It's easy to jam a syringe with tons of spores and get a jar to turn white in a few days.  However, many substrains are not compatible, so you'll end up with dozens or even hundreds of strains trying to share a small substrate.

We don't advertise those two ripoff places here.
RR


*****
Of course there's thousands of parents if thousands of spores germinate from multispore inoculation. Eventually, most of the substrains combine into a single organism through the process of anastomosis.

If one wishes to separate out the individual strains, or substrains as they're sometimes called in order to preserve the diversity, you want to place the multispore culture on agar where you can observe the growth in the flat, two dimensional plane of the Petri dish. This process needs to begin within a day or two of germination so you can catch the individual strains before they combine with other dikaryons via anastomosis.

Some strains are not compatible, so this is the reason on many substrates that were inoculated by multispore you'll see drastically different looking(and performing) mushrooms on the same flush. It's because those that are not compatible usually lay claim to their niche of real estate and fruit from there.
RR





*****
quote:
"but I think that dark purple syringes are a waste of spores"

Words of wisdom. Too many spores translates into too many substrains germinating. It matters little if it colonizes faster if a thousand substrains are competing for your small jar of food. There is more to this hobby than simply colonization times. I challenge anybody to produce a monster flush of mushrooms from a dark purple syringe.
RR


Edited by Aureus (08/24/15 03:36 PM)


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OfflinecronicrFacebook
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Re: Combining multispore cakes when making bulks [Re: Aureus]
    #22132454 - 08/23/15 07:07 PM (8 years, 5 months ago)

again he is not talking about mixing two variety's he is simply saying using toomuch spore solution


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleAureus
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Re: Combining multispore cakes when making bulks [Re: cronicr]
    #22132484 - 08/23/15 07:17 PM (8 years, 5 months ago)

A variety is just a substrain that has been stabilized.


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Re: Combining multispore cakes when making bulks [Re: Aureus]
    #22132496 - 08/23/15 07:21 PM (8 years, 5 months ago)

way too high for this:leaving:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleAureus
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Re: Combining multispore cakes when making bulks [Re: cronicr]
    #22132503 - 08/23/15 07:23 PM (8 years, 5 months ago)

Lol


Edited by Aureus (08/23/15 07:29 PM)


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OfflineGrim767
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Re: Combining multispore cakes when making bulks [Re: cronicr]
    #22133052 - 08/23/15 09:57 PM (8 years, 5 months ago)

Quote:

cronicr said:
i don't know where you are reading this but don't read there anymore



What he said.....


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