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OfflineCloudbase Jim
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I can grow mold like a pro!!
    #22131796 - 08/23/15 04:51 PM (8 years, 5 months ago)

Hi guys,

Just wondering if there is a specific agar tek for a glove box using a syringe?

So far all I have accomplished is a kaleidoscope of mold.
I usually spray a lot of alcohol around everything a half hour before i begin, then I make a pit in the agar and mix some syringe sauce in.
This has the effect of making a lot of condensation and mold everywhere but ... no mycelium.

I have left a petri dish for a week in the same place without trying to inoculate it and it has stayed contam free, so I know its an issue with my glove box/syringe tek.

Would love some valuable shrooomery input, thanks.

Oh, also the spores are from ******** could this be my problem?

Thanks


Edited by Cloudbase Jim (08/29/15 10:51 AM)


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InvisibleKush_Zombie
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131814 - 08/23/15 04:55 PM (8 years, 5 months ago)

Try to keep the box off of the floor, put it on a chair or table or something like that. I've also heard of people putting tin foil (or a certain kind of tape, I forget which) over the jars so that no contams can get in the holes that you made to put the syringe in. Hope this helps and goodluck! :smile:


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Edited by Kush_Zombie (08/23/15 05:11 PM)


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Offlinespacechildo
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131816 - 08/23/15 04:56 PM (8 years, 5 months ago)

rip the gloves off your box and call it a SAB instead,
use very little spore water on the agar, even better just squirt some on your loop and streak that.

spores101 is a major problem yes, still surprised the syringe wasnt just water tho.


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InvisibleInocuole
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Re: I can grow mold like a pro!! [Re: spacechildo]
    #22131827 - 08/23/15 04:57 PM (8 years, 5 months ago)

Can we not talk about sponsors in here?  This is a cultivation forum, and those are for microscopy.

http://www.shroomery.org/forums/showflat.php/Number/14960167


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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InvisibleKush_Zombie
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Re: I can grow mold like a pro!! *DELETED* [Re: Inocuole]
    #22131839 - 08/23/15 04:59 PM (8 years, 5 months ago)

Post deleted by Kush_Zombie

Reason for deletion: im a dick



--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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Offlinespacechildo
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Re: I can grow mold like a pro!! [Re: Inocuole]
    #22131845 - 08/23/15 05:00 PM (8 years, 5 months ago)

Quote:

Inocuole said:
Can we not talk about sponsors in here?  This is a cultivation forum, and those are for microscopy.

http://www.shroomery.org/forums/showflat.php/Number/14960167




agree but there's 2 guys who should be mentioned a LOT, especially when people ask,
and that's ralph and whores101. they're a scam and we only talk bad about them.

if you meant the other dude but just quick replied, forget all the above!


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131850 - 08/23/15 05:01 PM (8 years, 5 months ago)

you must use a sab for agar work



What you do you make your agar and pc it at 15 psi for half and hour let cool till the bottle is touchable with gloves on then unwrap the packet of dishes and you can place them on one of these to avoid direct contact with the bottom  (the black thing) then carefully take each dish pouring them and allowing them to cool while wrapped in syran wrap. Youll have to use it one sheet at a time ti avoid tilting the still hot agar.

Once cooled un wrap and begin to place a drop of spores on each of the plates then wrapping in paraflim or syran wrap.


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Invisiblemrbart4444
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Registered: 09/13/14
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Re: I can grow mold like a pro!! [Re: Kush_Zombie]
    #22131854 - 08/23/15 05:02 PM (8 years, 5 months ago)

Quote:

Kush_Zombie said:
Quote:

Inocuole said:
Can we not talk about sponsors in here?  This is a cultivation forum, and those are for microscopy.

http://www.shroomery.org/forums/showflat.php/Number/14960167



Thanks officer



QFT :cop: for sure


--------------------


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InvisibleKush_Zombie
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Re: I can grow mold like a pro!! [Re: mrbart4444]
    #22131856 - 08/23/15 05:03 PM (8 years, 5 months ago)

:llamastare:


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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OfflineCloudbase Jim
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Re: I can grow mold like a pro!! [Re: Inocuole]
    #22131863 - 08/23/15 05:04 PM (8 years, 5 months ago)

Wow! I brushed my teeth and had 3 replies! I f-ing love this forum.
Sorry Inocuole, I don't meant to step out of line... new to posting here.

The SAB thing interests me, does it not drastically increase the chance of introducing foreign bodies into your working environment?


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InvisibleInocuole
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131866 - 08/23/15 05:05 PM (8 years, 5 months ago)

More like drastically reduce.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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Offlinespacechildo
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131876 - 08/23/15 05:07 PM (8 years, 5 months ago)

how frank gets his shit done - you should take a look!

a SAB will always beat a GB from a plastic tub, its not air tight anyway
and you want to flame your tools outside the box to keep still air.

and re; the officer comments i'm fuckin glad inoc helps keep this place happening,
if we all talked about how to misuse sponsors spores and sell drugs etc this place would be closed in a second
and we'd all have to go to fuckin topia or some other lame site that's stuck in the 90s :facepalm:


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131884 - 08/23/15 05:08 PM (8 years, 5 months ago)

Quote:

Cloudbase Jim said:
Wow! I brushed my teeth and had 3 replies! I f-ing love this forum.
Sorry Inocuole, I don't meant to step out of line... new to posting here.

The SAB thing interests me, does it not drastically increase the chance of introducing foreign bodies into your working environment?



Not if the air in the box is still that means there is not as many particles floating around in the box than just open air . Which explains the mold you are growing


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InvisibleInocuole
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Re: I can grow mold like a pro!! [Re: spacechildo]
    #22131897 - 08/23/15 05:10 PM (8 years, 5 months ago)

Quote:

spacechildo said:
how frank gets his shit done - you should take a look!

a SAB will always beat a GB from a plastic tub, its not air tight anyway
and you want to flame your tools outside the box to keep still air.

and re; the officer comments i'm fuckin glad inoc helps keep this place happening,
if we all talked about how to misuse sponsors spores and sell drugs etc this place would be closed in a second
and we'd all have to go to fuckin topia or some other lame site that's stuck in the 90s :facepalm:




:seriousthankyou:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisibleKush_Zombie
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Re: I can grow mold like a pro!! [Re: spacechildo]
    #22131899 - 08/23/15 05:10 PM (8 years, 5 months ago)

Quote:

spacechildo said:
how frank gets his shit done - you should take a look!

a SAB will always beat a GB from a plastic tub, its not air tight anyway
and you want to flame your tools outside the box to keep still air.

and re; the officer comments i'm fuckin glad inoc helps keep this place happening,
if we all talked about how to misuse sponsors spores and sell drugs etc this place would be closed in a second
and we'd all have to go to fuckin topia or some other lame site that's stuck in the 90s :facepalm:



ah true, didn't think about that. I shall edit my comment to take the sponsor out
:nodofunderstanding:


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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OfflineCloudbase Jim
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Registered: 08/07/15
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Last seen: 8 years, 1 month
Re: I can grow mold like a pro!! [Re: Kush_Zombie]
    #22131928 - 08/23/15 05:16 PM (8 years, 5 months ago)

Sorry Mr Bart are you saying that SAB is better than a GB for keeping the area sterile? I have never used open air.
I must make it clear that the spores are for oyster mushrooms of course


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InvisibleInocuole
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131946 - 08/23/15 05:19 PM (8 years, 5 months ago)

Quote:

Cloudbase Jim said:
Sorry Mr Bart are you saying that SAB is better than a GB for keeping the area sterile? I have never used open air.
I must make it clear that the spores are for oyster mushrooms of course




Then you're looking for this forum:

http://www.shroomery.org/forums/postlist.php/Board/13

This forum is for psychoactive mushrooms only.  So either admit you're growing them or post in the correct forum.  And yes a SAB is better than a GB because you can't flame your tools outside of a GB, and that creates currents inside when you do, or you go without flaming your tools, which is bad.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Invisiblemrbart4444
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Registered: 09/13/14
Posts: 2,266
Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131950 - 08/23/15 05:20 PM (8 years, 5 months ago)

oh hell yea man glove boxes you cant even take tools out to flame and you cant flame in the box i made the mistake of thinking this when i first started getting into agar SABs are proven and work the best for me.
glove boxes look cool though


--------------------


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OfflineCloudbase Jim
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Re: I can grow mold like a pro!! [Re: Inocuole]
    #22131966 - 08/23/15 05:25 PM (8 years, 5 months ago)

sorry Inocuole. Please reading that whole sentence out loud in a British accent  emphasizing the "of course" at the end :wink:


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InvisibleInocuole
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131971 - 08/23/15 05:26 PM (8 years, 5 months ago)

Sorry I just don't have any patience for the "oh but they're oysters" line.  Heard it a million times, if they were really oysters, there's a forum for that.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflineCloudbase Jim
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131983 - 08/23/15 05:28 PM (8 years, 5 months ago)

Ok,
all taken on board and gloves have been removed from my GB.

Thanks for the advice


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
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Re: I can grow mold like a pro!! [Re: Inocuole]
    #22131987 - 08/23/15 05:28 PM (8 years, 5 months ago)

All i grow is oysters wtf are you doing incoule .. not something illegal id hope


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OfflineCloudbase Jim
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131993 - 08/23/15 05:29 PM (8 years, 5 months ago)

I only said that because I thought you had an issue with naming spore providers whilst discussing cultivation.

my bad


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InvisibleKush_Zombie
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22132001 - 08/23/15 05:31 PM (8 years, 5 months ago)

Lol welcome to the shroomery cloud :hug:
Don't worry, we all hear this bs at some point or another


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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OfflineCloudbase Jim
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Re: I can grow mold like a pro!! [Re: Kush_Zombie]
    #22132015 - 08/23/15 05:33 PM (8 years, 5 months ago)

Thanks Kush,
Glad to be part of the shroomery after watching from the sidelines for so long.


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Offlinespacechildo
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22132023 - 08/23/15 05:36 PM (8 years, 5 months ago)

Quote:

Cloudbase Jim said:
I only said that because I thought you had an issue with naming spore providers whilst discussing cultivation.

my bad




lol yeah, so just stop mentioning vendors, dont stop talking about cultivation :lol:

if you just start over with a new print I'm sure everything will go much better for ya,
spores101 isnt even a vendor its a scam site that is a parasite on mush cultivators. Send them a e-mail tell them to go to hell and die and just take it as a learning experience.


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OfflineCloudbase Jim
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22132028 - 08/23/15 05:36 PM (8 years, 5 months ago)

oh!
Is it still a good idea to go nuts with the alcohol spray in an SAB?


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Offlinespacechildo
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Re: I can grow mold like a pro!! [Re: spacechildo]
    #22132030 - 08/23/15 05:37 PM (8 years, 5 months ago)

just use soap and water, I think frank says so in his SAb tek..


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OfflineCloudbase Jim
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22132032 - 08/23/15 05:38 PM (8 years, 5 months ago)

hahaha yeah that email is already sent!

Took great enjoyment in it too but probably not $40 worth


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OfflineCloudbase Jim
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22132042 - 08/23/15 05:40 PM (8 years, 5 months ago)

Excellent.
Thanks for the help again guys.
Work in 5 hours so signing out here.
Peace to all star light harvesters


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Offlinebluegill
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22132048 - 08/23/15 05:40 PM (8 years, 5 months ago)

I'm about to make a SAB with a 56qt sterilite box and some PVC drain caps. Is it better to offset the arm holes or have them level?


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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Invisiblemrbart4444
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Registered: 09/13/14
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Re: I can grow mold like a pro!! [Re: bluegill]
    #22132118 - 08/23/15 05:53 PM (8 years, 5 months ago)

Quote:

bluegill said:
I'm about to make a SAB with a 56qt sterilite box and some PVC drain caps. Is it better to offset the arm holes or have them level?



shouldve got the 19 gal tub . Still a good sized tub thought just make arms level how far depends on you though i had to make a sab before i made my best one to see what worked for me .


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Offlinebluegill
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Re: I can grow mold like a pro!! [Re: mrbart4444]
    #22132296 - 08/23/15 06:33 PM (8 years, 5 months ago)

Maybe I should stick the arms one on each short side :P


--------------------
"Psychedelics are like carnival tickets, you buy the ticket and take the ride, then you get off and go home. What your talking about is physical death. That's when you're pulled into the carnival against your will and your stuck there for eternity."


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Invisibleelasticaltiger
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22132326 - 08/23/15 06:39 PM (8 years, 5 months ago)

Quote:

Cloudbase Jim said:
Hi guys,

Just wondering if there is a specific agar tek for a glove box using a syringe?

So far all I have accomplished is a kaleidoscope of mold.
I usually spray a lot of alcohol around everything a half hour before i begin, then I make a pit in the agar and mix some syringe sauce in.
This has the effect of making a lot of condensation and mold everywhere but ... no mycelium.

I have left a petri dish for a week in the same place without trying to inoculate it and it has stayed contam free, so I know its an issue with my glove box/syringe tek.

Would love some valuable shrooomery input, thanks.

Oh, also the spores are from spores101.com could this be my problem?

Thanks




How are you making a pit in the agar? That is your problem right there I think.

Forget the pit. Just put a single drop of solution on the dish and close immediately.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

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EZEKIEL 23:20


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OfflineCloudbase Jim
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Re: I can grow mold like a pro!! [Re: elasticaltiger]
    #22141853 - 08/25/15 04:57 PM (8 years, 5 months ago)

I usually try and embed the liquid into the agar by scratching the surface whilst injecting.
It is always my aim to not have any liquid sloping around on my agar so I only use a drop or so.

Appologies if this question is out of order but I was wondering if, In my circumstances would people here order new spores (bearing in mind that mine came from *******)

cheers


Edited by Cloudbase Jim (08/26/15 10:16 AM)


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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22142209 - 08/25/15 05:56 PM (8 years, 5 months ago)

cloudbase jim check your PM's and you should edit out the vendor names on this thread...


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