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OfflineCloudbase Jim
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Registered: 08/07/15
Posts: 46
Last seen: 8 years, 1 month
I can grow mold like a pro!!
    #22131796 - 08/23/15 04:51 PM (8 years, 5 months ago)

Hi guys,

Just wondering if there is a specific agar tek for a glove box using a syringe?

So far all I have accomplished is a kaleidoscope of mold.
I usually spray a lot of alcohol around everything a half hour before i begin, then I make a pit in the agar and mix some syringe sauce in.
This has the effect of making a lot of condensation and mold everywhere but ... no mycelium.

I have left a petri dish for a week in the same place without trying to inoculate it and it has stayed contam free, so I know its an issue with my glove box/syringe tek.

Would love some valuable shrooomery input, thanks.

Oh, also the spores are from ******** could this be my problem?

Thanks


Edited by Cloudbase Jim (08/29/15 10:51 AM)


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InvisibleKush_Zombie
smug piece of shit
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Registered: 10/22/14
Posts: 4,793
Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131814 - 08/23/15 04:55 PM (8 years, 5 months ago)

Try to keep the box off of the floor, put it on a chair or table or something like that. I've also heard of people putting tin foil (or a certain kind of tape, I forget which) over the jars so that no contams can get in the holes that you made to put the syringe in. Hope this helps and goodluck! :smile:


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Edited by Kush_Zombie (08/23/15 05:11 PM)


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Offlinespacechildo
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Posts: 19,243
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131816 - 08/23/15 04:56 PM (8 years, 5 months ago)

rip the gloves off your box and call it a SAB instead,
use very little spore water on the agar, even better just squirt some on your loop and streak that.

spores101 is a major problem yes, still surprised the syringe wasnt just water tho.


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InvisibleInocuole
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Posts: 24,863
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Re: I can grow mold like a pro!! [Re: spacechildo]
    #22131827 - 08/23/15 04:57 PM (8 years, 5 months ago)

Can we not talk about sponsors in here?  This is a cultivation forum, and those are for microscopy.

http://www.shroomery.org/forums/showflat.php/Number/14960167


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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InvisibleKush_Zombie
smug piece of shit
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Registered: 10/22/14
Posts: 4,793
Re: I can grow mold like a pro!! *DELETED* [Re: Inocuole]
    #22131839 - 08/23/15 04:59 PM (8 years, 5 months ago)

Post deleted by Kush_Zombie

Reason for deletion: im a dick



--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: I can grow mold like a pro!! [Re: Inocuole]
    #22131845 - 08/23/15 05:00 PM (8 years, 5 months ago)

Quote:

Inocuole said:
Can we not talk about sponsors in here?  This is a cultivation forum, and those are for microscopy.

http://www.shroomery.org/forums/showflat.php/Number/14960167




agree but there's 2 guys who should be mentioned a LOT, especially when people ask,
and that's ralph and whores101. they're a scam and we only talk bad about them.

if you meant the other dude but just quick replied, forget all the above!


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131850 - 08/23/15 05:01 PM (8 years, 5 months ago)

you must use a sab for agar work



What you do you make your agar and pc it at 15 psi for half and hour let cool till the bottle is touchable with gloves on then unwrap the packet of dishes and you can place them on one of these to avoid direct contact with the bottom  (the black thing) then carefully take each dish pouring them and allowing them to cool while wrapped in syran wrap. Youll have to use it one sheet at a time ti avoid tilting the still hot agar.

Once cooled un wrap and begin to place a drop of spores on each of the plates then wrapping in paraflim or syran wrap.


--------------------


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: I can grow mold like a pro!! [Re: Kush_Zombie]
    #22131854 - 08/23/15 05:02 PM (8 years, 5 months ago)

Quote:

Kush_Zombie said:
Quote:

Inocuole said:
Can we not talk about sponsors in here?  This is a cultivation forum, and those are for microscopy.

http://www.shroomery.org/forums/showflat.php/Number/14960167



Thanks officer



QFT :cop: for sure


--------------------


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InvisibleKush_Zombie
smug piece of shit
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Registered: 10/22/14
Posts: 4,793
Re: I can grow mold like a pro!! [Re: mrbart4444]
    #22131856 - 08/23/15 05:03 PM (8 years, 5 months ago)

:llamastare:


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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OfflineCloudbase Jim
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Registered: 08/07/15
Posts: 46
Last seen: 8 years, 1 month
Re: I can grow mold like a pro!! [Re: Inocuole]
    #22131863 - 08/23/15 05:04 PM (8 years, 5 months ago)

Wow! I brushed my teeth and had 3 replies! I f-ing love this forum.
Sorry Inocuole, I don't meant to step out of line... new to posting here.

The SAB thing interests me, does it not drastically increase the chance of introducing foreign bodies into your working environment?


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InvisibleInocuole
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Registered: 11/21/11
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131866 - 08/23/15 05:05 PM (8 years, 5 months ago)

More like drastically reduce.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Offlinespacechildo
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131876 - 08/23/15 05:07 PM (8 years, 5 months ago)

how frank gets his shit done - you should take a look!

a SAB will always beat a GB from a plastic tub, its not air tight anyway
and you want to flame your tools outside the box to keep still air.

and re; the officer comments i'm fuckin glad inoc helps keep this place happening,
if we all talked about how to misuse sponsors spores and sell drugs etc this place would be closed in a second
and we'd all have to go to fuckin topia or some other lame site that's stuck in the 90s :facepalm:


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131884 - 08/23/15 05:08 PM (8 years, 5 months ago)

Quote:

Cloudbase Jim said:
Wow! I brushed my teeth and had 3 replies! I f-ing love this forum.
Sorry Inocuole, I don't meant to step out of line... new to posting here.

The SAB thing interests me, does it not drastically increase the chance of introducing foreign bodies into your working environment?



Not if the air in the box is still that means there is not as many particles floating around in the box than just open air . Which explains the mold you are growing


--------------------


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InvisibleInocuole
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Registered: 11/21/11
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Re: I can grow mold like a pro!! [Re: spacechildo]
    #22131897 - 08/23/15 05:10 PM (8 years, 5 months ago)

Quote:

spacechildo said:
how frank gets his shit done - you should take a look!

a SAB will always beat a GB from a plastic tub, its not air tight anyway
and you want to flame your tools outside the box to keep still air.

and re; the officer comments i'm fuckin glad inoc helps keep this place happening,
if we all talked about how to misuse sponsors spores and sell drugs etc this place would be closed in a second
and we'd all have to go to fuckin topia or some other lame site that's stuck in the 90s :facepalm:




:seriousthankyou:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisibleKush_Zombie
smug piece of shit
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Registered: 10/22/14
Posts: 4,793
Re: I can grow mold like a pro!! [Re: spacechildo]
    #22131899 - 08/23/15 05:10 PM (8 years, 5 months ago)

Quote:

spacechildo said:
how frank gets his shit done - you should take a look!

a SAB will always beat a GB from a plastic tub, its not air tight anyway
and you want to flame your tools outside the box to keep still air.

and re; the officer comments i'm fuckin glad inoc helps keep this place happening,
if we all talked about how to misuse sponsors spores and sell drugs etc this place would be closed in a second
and we'd all have to go to fuckin topia or some other lame site that's stuck in the 90s :facepalm:



ah true, didn't think about that. I shall edit my comment to take the sponsor out
:nodofunderstanding:


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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OfflineCloudbase Jim
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Registered: 08/07/15
Posts: 46
Last seen: 8 years, 1 month
Re: I can grow mold like a pro!! [Re: Kush_Zombie]
    #22131928 - 08/23/15 05:16 PM (8 years, 5 months ago)

Sorry Mr Bart are you saying that SAB is better than a GB for keeping the area sterile? I have never used open air.
I must make it clear that the spores are for oyster mushrooms of course


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InvisibleInocuole
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131946 - 08/23/15 05:19 PM (8 years, 5 months ago)

Quote:

Cloudbase Jim said:
Sorry Mr Bart are you saying that SAB is better than a GB for keeping the area sterile? I have never used open air.
I must make it clear that the spores are for oyster mushrooms of course




Then you're looking for this forum:

http://www.shroomery.org/forums/postlist.php/Board/13

This forum is for psychoactive mushrooms only.  So either admit you're growing them or post in the correct forum.  And yes a SAB is better than a GB because you can't flame your tools outside of a GB, and that creates currents inside when you do, or you go without flaming your tools, which is bad.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131950 - 08/23/15 05:20 PM (8 years, 5 months ago)

oh hell yea man glove boxes you cant even take tools out to flame and you cant flame in the box i made the mistake of thinking this when i first started getting into agar SABs are proven and work the best for me.
glove boxes look cool though


--------------------


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OfflineCloudbase Jim
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Registered: 08/07/15
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Last seen: 8 years, 1 month
Re: I can grow mold like a pro!! [Re: Inocuole]
    #22131966 - 08/23/15 05:25 PM (8 years, 5 months ago)

sorry Inocuole. Please reading that whole sentence out loud in a British accent  emphasizing the "of course" at the end :wink:


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InvisibleInocuole
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Re: I can grow mold like a pro!! [Re: Cloudbase Jim]
    #22131971 - 08/23/15 05:26 PM (8 years, 5 months ago)

Sorry I just don't have any patience for the "oh but they're oysters" line.  Heard it a million times, if they were really oysters, there's a forum for that.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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