|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Cloudbase Jim
Stranger


Registered: 08/07/15
Posts: 46
Last seen: 8 years, 1 month
|
I can grow mold like a pro!!
#22131796 - 08/23/15 04:51 PM (8 years, 5 months ago) |
|
|
Hi guys,
Just wondering if there is a specific agar tek for a glove box using a syringe?
So far all I have accomplished is a kaleidoscope of mold. I usually spray a lot of alcohol around everything a half hour before i begin, then I make a pit in the agar and mix some syringe sauce in. This has the effect of making a lot of condensation and mold everywhere but ... no mycelium.
I have left a petri dish for a week in the same place without trying to inoculate it and it has stayed contam free, so I know its an issue with my glove box/syringe tek.
Would love some valuable shrooomery input, thanks.
Oh, also the spores are from ******** could this be my problem?
Thanks
Edited by Cloudbase Jim (08/29/15 10:51 AM)
|
Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
|
|
Try to keep the box off of the floor, put it on a chair or table or something like that. I've also heard of people putting tin foil (or a certain kind of tape, I forget which) over the jars so that no contams can get in the holes that you made to put the syringe in. Hope this helps and goodluck!
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
Edited by Kush_Zombie (08/23/15 05:11 PM)
|
spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
|
rip the gloves off your box and call it a SAB instead, use very little spore water on the agar, even better just squirt some on your loop and streak that.
spores101 is a major problem yes, still surprised the syringe wasnt just water tho.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: I can grow mold like a pro!! [Re: spacechildo]
#22131827 - 08/23/15 04:57 PM (8 years, 5 months ago) |
|
|
Can we not talk about sponsors in here? This is a cultivation forum, and those are for microscopy.
http://www.shroomery.org/forums/showflat.php/Number/14960167
|
Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
|
Re: I can grow mold like a pro!! *DELETED* [Re: Inocuole]
#22131839 - 08/23/15 04:59 PM (8 years, 5 months ago) |
|
|
Post deleted by Kush_ZombieReason for deletion: im a dick
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
|
spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: I can grow mold like a pro!! [Re: Inocuole]
#22131845 - 08/23/15 05:00 PM (8 years, 5 months ago) |
|
|
Quote:
Inocuole said: Can we not talk about sponsors in here? This is a cultivation forum, and those are for microscopy.
http://www.shroomery.org/forums/showflat.php/Number/14960167
agree but there's 2 guys who should be mentioned a LOT, especially when people ask, and that's ralph and whores101. they're a scam and we only talk bad about them.
if you meant the other dude but just quick replied, forget all the above!
|
mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
|
|
you must use a sab for agar work 
What you do you make your agar and pc it at 15 psi for half and hour let cool till the bottle is touchable with gloves on then unwrap the packet of dishes and you can place them on one of these to avoid direct contact with the bottom (the black thing) then carefully take each dish pouring them and allowing them to cool while wrapped in syran wrap. Youll have to use it one sheet at a time ti avoid tilting the still hot agar.
Once cooled un wrap and begin to place a drop of spores on each of the plates then wrapping in paraflim or syran wrap.
--------------------
|
mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
|
Re: I can grow mold like a pro!! [Re: Kush_Zombie]
#22131854 - 08/23/15 05:02 PM (8 years, 5 months ago) |
|
|
Quote:
Kush_Zombie said:
Quote:
Inocuole said: Can we not talk about sponsors in here? This is a cultivation forum, and those are for microscopy.
http://www.shroomery.org/forums/showflat.php/Number/14960167
Thanks officer
QFT for sure
--------------------
|
Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
|
Re: I can grow mold like a pro!! [Re: mrbart4444]
#22131856 - 08/23/15 05:03 PM (8 years, 5 months ago) |
|
|
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
|
Cloudbase Jim
Stranger


Registered: 08/07/15
Posts: 46
Last seen: 8 years, 1 month
|
Re: I can grow mold like a pro!! [Re: Inocuole]
#22131863 - 08/23/15 05:04 PM (8 years, 5 months ago) |
|
|
Wow! I brushed my teeth and had 3 replies! I f-ing love this forum. Sorry Inocuole, I don't meant to step out of line... new to posting here.
The SAB thing interests me, does it not drastically increase the chance of introducing foreign bodies into your working environment?
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
|
More like drastically reduce.
|
spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
|
how frank gets his shit done - you should take a look!
a SAB will always beat a GB from a plastic tub, its not air tight anyway and you want to flame your tools outside the box to keep still air.
and re; the officer comments i'm fuckin glad inoc helps keep this place happening, if we all talked about how to misuse sponsors spores and sell drugs etc this place would be closed in a second and we'd all have to go to fuckin topia or some other lame site that's stuck in the 90s
|
mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
|
|
Quote:
Cloudbase Jim said: Wow! I brushed my teeth and had 3 replies! I f-ing love this forum. Sorry Inocuole, I don't meant to step out of line... new to posting here.
The SAB thing interests me, does it not drastically increase the chance of introducing foreign bodies into your working environment?
Not if the air in the box is still that means there is not as many particles floating around in the box than just open air . Which explains the mold you are growing
--------------------
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: I can grow mold like a pro!! [Re: spacechildo]
#22131897 - 08/23/15 05:10 PM (8 years, 5 months ago) |
|
|
Quote:
spacechildo said: how frank gets his shit done - you should take a look!
a SAB will always beat a GB from a plastic tub, its not air tight anyway and you want to flame your tools outside the box to keep still air.
and re; the officer comments i'm fuckin glad inoc helps keep this place happening, if we all talked about how to misuse sponsors spores and sell drugs etc this place would be closed in a second and we'd all have to go to fuckin topia or some other lame site that's stuck in the 90s 
|
Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
|
Re: I can grow mold like a pro!! [Re: spacechildo]
#22131899 - 08/23/15 05:10 PM (8 years, 5 months ago) |
|
|
Quote:
spacechildo said: how frank gets his shit done - you should take a look!
a SAB will always beat a GB from a plastic tub, its not air tight anyway and you want to flame your tools outside the box to keep still air.
and re; the officer comments i'm fuckin glad inoc helps keep this place happening, if we all talked about how to misuse sponsors spores and sell drugs etc this place would be closed in a second and we'd all have to go to fuckin topia or some other lame site that's stuck in the 90s 
ah true, didn't think about that. I shall edit my comment to take the sponsor out
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
|
Cloudbase Jim
Stranger


Registered: 08/07/15
Posts: 46
Last seen: 8 years, 1 month
|
Re: I can grow mold like a pro!! [Re: Kush_Zombie]
#22131928 - 08/23/15 05:16 PM (8 years, 5 months ago) |
|
|
Sorry Mr Bart are you saying that SAB is better than a GB for keeping the area sterile? I have never used open air. I must make it clear that the spores are for oyster mushrooms of course
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
|
Quote:
Cloudbase Jim said: Sorry Mr Bart are you saying that SAB is better than a GB for keeping the area sterile? I have never used open air. I must make it clear that the spores are for oyster mushrooms of course
Then you're looking for this forum:
http://www.shroomery.org/forums/postlist.php/Board/13
This forum is for psychoactive mushrooms only. So either admit you're growing them or post in the correct forum. And yes a SAB is better than a GB because you can't flame your tools outside of a GB, and that creates currents inside when you do, or you go without flaming your tools, which is bad.
|
mrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
|
|
oh hell yea man glove boxes you cant even take tools out to flame and you cant flame in the box i made the mistake of thinking this when i first started getting into agar SABs are proven and work the best for me.  glove boxes look cool though
--------------------
|
Cloudbase Jim
Stranger


Registered: 08/07/15
Posts: 46
Last seen: 8 years, 1 month
|
Re: I can grow mold like a pro!! [Re: Inocuole]
#22131966 - 08/23/15 05:25 PM (8 years, 5 months ago) |
|
|
sorry Inocuole. Please reading that whole sentence out loud in a British accent emphasizing the "of course" at the end
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
|
Sorry I just don't have any patience for the "oh but they're oysters" line. Heard it a million times, if they were really oysters, there's a forum for that.
|
|