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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 21 days
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1 Jar Spawn
#22130382 - 08/23/15 10:51 AM (8 years, 5 months ago) |
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I had gone ahead and used my last syringe of Thai spores and inoculated about 5 jars. Sadly, due to the abnormal heatwaves, only 1 jar has managed to make it. The jar is 450ml of spawn
What I was thinking of doing is prep some old fashioned CVG substrate and spawn it in a 5L tote box with bin liner. Than put that tote box in an empty monotub with usual procedure; Taped up holes - wait for substrate to colonize - fill holes with polyfill for fruiting.
My question is would this work?
From my experience I don't see why not since it's basically the same as when you fill up a whole mono but then again I am no expert and I am not sure if the surrounding empty space around it would have any negative effects.
I am currently not at my usual place so I do not have access to my SAB or any other useful tools and I am not particularly interested in doing agar work with this one so let's get that option out of the way.
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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PoIIo
Stranger
Registered: 08/05/15
Posts: 83
Last seen: 8 years, 1 month
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With 1 jar of spawn I would consider a mini hybrid tub. Here is an extensive thread.
http://www.shroomery.org/forums/showflat.php/Number/10713572/fpart/1/vc/1
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: 1 Jar Spawn [Re: PoIIo]
#22130434 - 08/23/15 11:11 AM (8 years, 5 months ago) |
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Grain to grain?
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psilocybist
All is One



Registered: 05/22/14
Posts: 528
Loc: Far
Last seen: 1 day, 9 hours
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if that strain is important to you, or it's the last living myc to produce mushrooms, I would G2G it, to make more spawn.( needs SAB) then you can it make it to fruits any way you want/are able. cause with one jar, if you fail to fruit successfully (any reason), you lose the second chance and so on.
you can also make GLC out of it, and inoculate more sterilized jars. this way you don't need a SAB, but PC is required anyway. 
hope it helps
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 21 days
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Its not that important. It was just the last syringe and i just decided fuck it let's try even though I was aware of the heat. I'm buying another pack soon and got some spore prints to work with.
Think I'll fruit it. There is a party soon and would like to shroom up with my girl for now.
Hmmm could you link me some tek for LC please?
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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Psilosopherr
A psilly goose



Registered: 02/15/12
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I have been doing what you described in the OP. works fine for me. (In that I put small tubs of colonized bulk substrate inside a monotub to fruit.)
Just because there's only a little spawn doesn't change anything, just adjust accordingly. Though G2G would be recommended.
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psilocybist
All is One



Registered: 05/22/14
Posts: 528
Loc: Far
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Quote:
Rosen_Rot said: Hmmm could you link me some tek for LC please?
that's GLC here's one and here another from TL
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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make agar recipe without the agar, fill in jar and PC. inoc with a clean wedge. voila!
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Appalachian Brony
Psilocyan Gosling

Registered: 03/23/15
Posts: 221
Loc: Dirty hills
Last seen: 4 years, 4 months
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If you going to make LC I would go ahead and make some agar plates as well and after you g2g stick a grain on their so you can keep the culture goin.
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psilocybist
All is One



Registered: 05/22/14
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Quote:
spacechildo said: make agar recipe without the agar, fill in jar and PC. inoc with a clean wedge. voila!
He has a colonized jar. and said agar is not his primary interest/choice/option. clean wedge ?!!!
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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then he shouldn't fuck with LC 
he really should start fuckin with agar tho!
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psilocybist
All is One



Registered: 05/22/14
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Last seen: 1 day, 9 hours
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Quote:
spacechildo said: then he shouldn't fuck with LC 
he really should start fuckin with agar tho!
in total agreement man
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 21 days
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I am not making LCs nor fucking around with agar atm. I asked simply for my own knowledge for further grows as well as to add to my notes. I've got other spore syringes on the way and a couple of prints which I want to fuck around with agar. This however will be done once I move back to my own pad and got my equipment as well as the temperature has bumped down a bit.
This batch had no plans. I just wanted some shrooms for me and my girl, it was the last syringe in the pack and I just thought fuck it, let's cultivate this shit. The temperature was not ideal and I am not at my usual place either but this one jar was persistent and it developed.
Anyway I just pastuerized some CVG, after an hour in the hoodie I went to check the temperature. Mudafuka's notes say it should be around 140F but mine was 151.5F.
Is this still good?
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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yep, CVG doesnt care about temps, and proper temps is 140-160 for pasteurization so you're good! you can even bucket tek it or sterilize it. just add heat!
re; the LC I was being serious when I said just make your favorite agar recipe and leave the agar out.
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 21 days
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Yes I know you were That was for the GLCs though right?
This is my little grow. Its a 5ltr ice-cream tub covered in bin-liner. If I cover the top with a lid loosely I don't think I would need a monotub for now since basically I need to wait for the CO2 to build up right?
Strain: P. cubensis Thai Ratio 1:2 ( I gave it a little extra on the top as casing but some grains are still exposed ) Substrate - CVG
Here goes nothing
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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no, that's just how you make the LC. GLC is LC with the added possibility of grains' endospores fuckin you up.
just cover the tray with alu foil and let it colonize on a shelf or something.
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Rosen_Rot
Learning



Registered: 12/06/14
Posts: 1,225
Loc: Goa
Last seen: 11 months, 21 days
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Quote:
spacechildo said: no, that's just how you make the LC. GLC is LC with the added possibility of grains' endospores fuckin you up.
just cover the tray with alu foil and let it colonize on a shelf or something.
solid advice bro
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"The internet has one rule; use or be used" - Bjeldiablo ''there is no loneliness, only moments where contentment is fleeting'' SBJs "The Basics" 3iRiS9 "Cirque du freak" B+ BONANZA
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