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OfflineAvatar5
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22162697 - 08/29/15 10:07 PM (8 years, 4 months ago)

Quote:

Avatar5 said:
Quote:

elasticaltiger said:
Don't Tell anyone. Don't take pictures. Sign up for a cheap VPN To connect to shroomery through. Don't give them to your regular weed dealer. Try to stick with people you have no affiliation with, preferably in another county. It will probably take you a year or two to meet someone not suspect and not associated with you directly.

Don't plan on getting rich selling cubensis. It never works out like you think it's going to and no one ever wants to pay what you want for the quantity you want to sell.



I think you totally misunderstood.  I never have and never will sell these to anyone under any circumstances.



I plan on posting pics actually already took some of my prep work. Just can't figure out how to attach photos here from a local source do you have to upload them to a website first Then give the link? Main thing I will need when this is done is a sitter I remember ayahuasca without and I feel that was a mistake. Hopefully someone from the forum will be able to talk to me via voip. I don't trust anyone in the town I'm in and feel telling anyone around here would be foolish. Also if the grow is successful I worry about dosage. I weight about 230 pounds. Do you guys think 2 grams dry is good to start with?


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InvisibleInocuole
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22162716 - 08/29/15 10:15 PM (8 years, 4 months ago)

http://www.shroomery.org/forums/upload.php

While you're there you'll probably notice Pics at the top of the page under your name.  :thumbup:  You'll figure it out from there I imagine.  You should be using the Threads button up there anyway to track your topics.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineAvatar5
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Re: Need Advice, unique situation. [Re: Inocuole]
    #22163088 - 08/30/15 12:01 AM (8 years, 4 months ago)

http:/ Here's some pics of the prep.


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InvisibleInocuole
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22163097 - 08/30/15 12:09 AM (8 years, 4 months ago)

Nice.  So you're doing a steam sterilization then?


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineAvatar5
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Re: Need Advice, unique situation. [Re: Inocuole]
    #22163113 - 08/30/15 12:16 AM (8 years, 4 months ago)

Yes already did. Did 2 Batches since all 4 containers wouldn't fit.about 80 mins a piece in boiling water. I got a 10 ml syringe so put 2.5 ml of p.cubensis "Escondido" into each trying to aim for the 4 corners so I could see growth.


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InvisibleInocuole
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22163122 - 08/30/15 12:20 AM (8 years, 4 months ago)

You used a still air box for that right?


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflineAvatar5
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Re: Need Advice, unique situation. [Re: Inocuole]
    #22163144 - 08/30/15 12:29 AM (8 years, 4 months ago)

Quote:

Inocuole said:
You used a still air box for that right?



Nope:( one part that I'm ashamed of. I used a closet where there was the least air movement possible but no sab. I took a shower and have fresh clothes and washed my hands well but I expect to lose 1-2 at least to contamination. The worst part is every night at work I have a fume hood but taking these in to work would be crazy.


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InvisibleInocuole
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22163145 - 08/30/15 12:30 AM (8 years, 4 months ago)

:facepalm:

If you only lost 1 or 2 from doing that, it'd be a miracle.  Sorry but... you might wanna get something else started rather than trust in those.  If shame were the worst to come of it that'd be fine but nobody likes wasting time. :shrug:

The proper still air box design and procedure is absolutely necessary for inoculating containers like those, at least without a SHIP.


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OfflineAvatar5
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Re: Need Advice, unique situation. [Re: Inocuole]
    #22163167 - 08/30/15 12:42 AM (8 years, 4 months ago)

It's no problem I've got plenty of substrate left. I can just empty them out and start again if things go bad. How long appoximately will the mycelium take to be noticeable? I can order more spores and see of I get lucky with these.


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OfflineAvatar5
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22165270 - 08/30/15 03:46 PM (8 years, 4 months ago)

here's hoping I get at least one that comes though. Has anyone else done it this way and been successful?


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Offlinetetherface
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22165301 - 08/30/15 03:55 PM (8 years, 4 months ago)

my first grow was pf tek inoculated in my moms kitchen via ms syringe and it worked but its not a good habit to get into


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OfflineAvatar5
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Re: Need Advice, unique situation. [Re: tetherface]
    #22165330 - 08/30/15 04:02 PM (8 years, 4 months ago)

Quote:

tetherface said:
my first grow was pf tek inoculated in my moms kitchen via ms syringe and it worked but its not a good habit to get into



I'm a scientist and so I really prefer to do things in a sterile and controlled manner however if the spores really couldn't compete with several air spores of other species how would they compete in nature? This intrigues me. Glad to know there is some hope.


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OfflinecronicrFacebook
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22165338 - 08/30/15 04:04 PM (8 years, 4 months ago)

Quote:

Avatar5 said:
Quote:

tetherface said:
my first grow was pf tek inoculated in my moms kitchen via ms syringe and it worked but its not a good habit to get into



I'm a scientist and so I really prefer to do things in a sterile and controlled manner however if the spores really couldn't compete with several air spores of other species how would they compete in nature? This intrigues me. Glad to know there is some hope.



they don't compete in nature, they take what they can get which is why our results indoors are so much better, a wild fruit is full of contams


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InvisibleInocuole
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22165347 - 08/30/15 04:05 PM (8 years, 4 months ago)

Quote:

Avatar5 said:
Quote:

tetherface said:
my first grow was pf tek inoculated in my moms kitchen via ms syringe and it worked but its not a good habit to get into



I'm a scientist and so I really prefer to do things in a sterile and controlled manner however if the spores really couldn't compete with several air spores of other species how would they compete in nature? This intrigues me. Glad to know there is some hope.




Because you're not expecting them to race other contams on free, sterilized, extremely nutritious food.

Nothing outside is ever sterilized and they have to have formed somewhat of a decent sized colony in order to not be in constant threat of getting wiped out.

Doing PF tek, in the classic sense, in open air, can actually be done, but not if you cracked the lid and injected that way.  Violet's tek requires the SAB, assuming that's what that is.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflineAvatar5
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Re: Need Advice, unique situation. [Re: Inocuole]
    #22165392 - 08/30/15 04:19 PM (8 years, 4 months ago)

Quote:

Inocuole said:
Quote:

Avatar5 said:
Quote:

tetherface said:
my first grow was pf tek inoculated in my moms kitchen via ms syringe and it worked but its not a good habit to get into



I'm a scientist and so I really prefer to do things in a sterile and controlled manner however if the spores really couldn't compete with several air spores of other species how would they compete in nature? This intrigues me. Glad to know there is some hope.




Because you're not expecting them to race other contams on free, sterilized, extremely nutritious food.

Nothing outside is ever sterilized and they have to have formed somewhat of a decent sized colony in order to not be in constant threat of getting wiped out.

Doing PF tek, in the classic sense, in open air, can actually be done, but not if you cracked the lid and injected that way.  Violet's tek requires the SAB, assuming that's what that is.



"For very beginners, a short squirt from a clean spore syringe can be squirted by lifting the edge of the lid and squirting spores in thru the gap.
This is best done in a still-air box, but has a good chance of success in any still-air condition away from carpets and rugs, or immediately after (even during) a rain outside."
Here's a piece of his tek where he talks about this. Don't be fooled I've worked with a fume hood and agar for my job daily,I understand there was some inherent risk in even a few seconds of air. Blood and other very potent agars I've noticed have about 25%-50% chance of development of containmention from the same air exposure. Although not exactly the same I think perhaps some basic premise may be applied here. Perhaps I am over hopeful and will have to try again.


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OfflinePussyFart
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22165416 - 08/30/15 04:27 PM (8 years, 4 months ago)

We use laminar flow hoods, not fume hoods.....


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OfflineAvatar5
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Re: Need Advice, unique situation. [Re: PussyFart]
    #22166115 - 08/30/15 07:31 PM (8 years, 4 months ago)

Quote:

PussyFart said:
We use laminar flow hoods, not fume hoods.....



Under the wiki for fume hoods..."A general but non-specific term for some of these local ventilation devices is Laminar flow cabinet."


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OfflinePussyFart
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Re: Need Advice, unique situation. [Re: Avatar5]
    #22166384 - 08/30/15 08:20 PM (8 years, 4 months ago)

Quote:

Avatar5 said:
Quote:

PussyFart said:
We use laminar flow hoods, not fume hoods.....



Under the wiki for fume hoods..."A general but non-specific term for some of these local ventilation devices is Laminar flow cabinet."



First off, wiki sucks....

Secondly, that is wrong.....a laminar flow hood pushes out a sterile air flow across what you are working on....

A fume hood is made to suck up fumes, which means dirty air is being sucked right past what u are working on to get to the filter....

Now that U know the "exact specific" term/definition, instead of a "general but non-specific" term for both, you can come to your own conclusion on which one is best suited for this hobby.


Edited by PussyFart (08/30/15 08:21 PM)


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Invisibleblindingleaf
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Re: Need Advice, unique situation. [Re: PussyFart]
    #22166620 - 08/30/15 08:52 PM (8 years, 4 months ago)

ya, fume hood a no no.
u can have vertical and horizontal hoods though.  one blows at you, the other blows down on ur work from above.


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OfflineAvatar5
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Re: Need Advice, unique situation. [Re: PussyFart]
    #22166654 - 08/30/15 08:57 PM (8 years, 4 months ago)

Quote:

PussyFart said:
Quote:

Avatar5 said:
Quote:

PussyFart said:
We use laminar flow hoods, not fume hoods.....



Under the wiki for fume hoods..."A general but non-specific term for some of these local ventilation devices is Laminar flow cabinet."



First off, wiki sucks....

Secondly, that is wrong.....a laminar flow hood pushes out a sterile air flow across what you are working on....

A fume hood is made to suck up fumes, which means dirty air is being sucked right past what u are working on to get to the filter....

Now that U know the "exact specific" term/definition, instead of a "general but non-specific" term for both, you can come to your own conclusion on which one is best suited for this hobby.



I understand what your saying. What I'm trying to articulate is this paticular device is used to insure sterile conditions for subculturing on agar. It is sterilized both chemically with alcohol everyday as well as with a uv blacklight for 1 hour. It has air filtration as well as a negative pressure environment therefore any free spores would simply be sucked away from your workspace. Unless you are working in a biochemistry lab or at the cdc or have a budget of over 100,000 dollars I think it's better then what 99% of private homes would have.


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