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Offlinejivangilad
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Make syringe from colonized jar?
    #22127291 - 08/22/15 02:28 PM (8 years, 5 months ago)

I thought to inject water into colonized rye from a syringe. mix jar and then retake  by syringe. Is this possible?

Seems simpler and safer for me then building glove box, for G2G.


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OfflinecronicrFacebook
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Re: Make syringe from colonized jar? [Re: jivangilad]
    #22127547 - 08/22/15 03:30 PM (8 years, 5 months ago)

yep but do this in a sab


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OfflinePsilosoulful

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Re: Make syringe from colonized jar? [Re: cronicr]
    #22127879 - 08/22/15 04:52 PM (8 years, 5 months ago)

If you are using a polyfill filter on your lid for gas exchange, then this can invite contams into the solution when you go to shake the jar. I've seen it done with SFD's successfully, but make sure to do it in an SAB like cronicr said.


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Offlinejivangilad
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Re: Make syringe from colonized jar? [Re: Psilosoulful]
    #22129127 - 08/22/15 11:17 PM (8 years, 5 months ago)

Quote:

Psilosoulful said:
If you are using a polyfill filter on your lid for gas exchange, then this can invite contams into the solution when you go to shake the jar. I've seen it done with SFD's successfully, but make sure to do it in an SAB like cronicr said.



I use Tyvek. I also have one hole covered with silicon, and I thought to inject the water through there.
I was hoping to avoid building a glove box, but you say I will need to ?


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InvisibleMad Season
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Re: Make syringe from colonized jar? [Re: jivangilad]
    #22129155 - 08/22/15 11:25 PM (8 years, 5 months ago)



In this video he uses a small 60 qt tub. Get 100+ qts. (Biggest clear tote you can find) you won't regret it.

Takes like less than 5 minutes to make. NO GLOVES. DON'T USE A GLOVE BOX. USE A STILL AIR BOX.


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OfflineGerG
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Re: Make syringe from colonized jar? [Re: Mad Season]
    #22129215 - 08/22/15 11:43 PM (8 years, 5 months ago)

You could do it without the GB or SAB, but you're taking a bigger risk of contamination. I, however, do open-air inoculation and g2g transfers. But, after I cover the (already filtered) in my grow area, and Oust the air, the entire room is like a SAB in about 10 minutes. I have never had a single contaminated jar doing shit this way. I may, however, just be an extremely lucky son of a bitch. With that said, I do own a SAB.

But, it's so easy and cheap to build a SAB, why not just do it and save yourself a lot of trouble in the long run?


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InvisibleMad Season
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Re: Make syringe from colonized jar? [Re: GerG]
    #22129275 - 08/23/15 12:19 AM (8 years, 5 months ago)

Open air g2ging.. :lol: oh jeez. And you only wait 10 minutes after spraying garbage spray?.. Generally in the air every inch has over 200,000 particles. On your skin every milimeter has 500000 particles. It takes a particle at 5 microns, 5 feet in the air 1 hour to reach the ground. I just don't see why you'd risk open air g2ging is all. Clean grains are extremely essential for success, and I've tossed pretty big projects because of stupid stuff like open air work.

Also if your room was truly still.. could you confidently say that after 6 months - a year.. left untouched exactly the way you would when working in... it would not be covered in dust? A still room wouldn't be covered in dust. It'd be exactly how you left it.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Invisibletahoe
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Re: Make syringe from colonized jar? [Re: Psilosoulful]
    #22129293 - 08/23/15 12:31 AM (8 years, 5 months ago)

Quote:

Psilosoulful said:
If you are using a polyfill filter on your lid for gas exchange, then this can invite contams into the solution when you go to shake the jar. I've seen it done with SFD's successfully, but make sure to do it in an SAB like cronicr said.




Are you saying that it can't or shouldn't be done if you are using poly fill as a filter? Your wording is funky


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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OfflinecronicrFacebook
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Re: Make syringe from colonized jar? [Re: tahoe]
    #22129296 - 08/23/15 12:32 AM (8 years, 5 months ago)

what does poly have anythign to do with it anyway? it's  a filter like any other:shrug:


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It doesn't matter what i think of you...all that matters is clean spawn

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Invisibletahoe
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Re: Make syringe from colonized jar? [Re: cronicr]
    #22129335 - 08/23/15 01:03 AM (8 years, 5 months ago)

Well yeah. Plus it can be done very easily with polyfill. I prefer polyfill for my lc's. Sure it can get messy and loaded up with living mycelium buy at least I know it's doing it's job and filtering the air.

I have made mycelium syringes by filling a syringe with sterile water. Injecting it into a fully colonized grain jar. Then sucking as much liquid up at possible. A 30+cc syringe and a long needle are ideal.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinejivangilad
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Re: Make syringe from colonized jar? [Re: tahoe]
    #22129491 - 08/23/15 02:43 AM (8 years, 5 months ago)

Quote:

tahoe said:
I have made mycelium syringes by filling a syringe with sterile water. Injecting it into a fully colonized grain jar. Then sucking as much liquid up at possible. A 30+cc syringe and a long needle are ideal.




Did you do it in a SAB?


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Invisibletahoe
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Re: Make syringe from colonized jar? [Re: jivangilad]
    #22129679 - 08/23/15 06:01 AM (8 years, 5 months ago)

Naw. It might help but no real need to. You leave the lid on the colonized jar. Work through the polyfill.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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