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Offlinemushroomnoob1981
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Stupid LC question
    #22125729 - 08/22/15 07:06 AM (8 years, 5 months ago)

I have a stupid question about liquid culture.  I know that when a rice cake or spawn jar becomes fully colonized that it no longer requires strict sterile technique because the myc can "fight off" a lot of contamination. 

Does the same apply to liquid culture (i.e. does a LC jar filled with myc still need to be handled as if it were LB broth with no antibiotic?)


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OfflinetaGyo
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Re: Stupid LC question [Re: mushroomnoob1981]
    #22125738 - 08/22/15 07:10 AM (8 years, 5 months ago)

No it doesn't. A spec of mold spore or bacteria in the highly nutritious LC water will cause it to multiply exponentially.

Always handle LCs with care. It's not that the myc can fight off contams so much as the contams have nothing to colonize on the substrate as the mycelium has a full grasp on all nutrients. In an LC nutrients are not in full grasp by the mycelium.


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