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OfflineYerow
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Help me choose my Agar recipe.
    #22121329 - 08/21/15 09:04 AM (8 years, 5 months ago)

Hey guys/girls :smile:

So i have been buying premade agar mix from one of our sponsors for a long time now, mostly because easy, but also because agar was a bitch to find in my area, even after scouting through our few asian markets and whatnot.

Finally i've found a pretty cheap deal online from a company a few cities over but now wondering about the recipe, i never really thought this far :shrug:

I've glimpsed through most of the related threads and i got a pretty good idea about what i'll be using.

I have:
Agar agar
Malt extract
Water from hydrating/boiling rye
Karo sirup (used this for lc's long ago)
Water from hydrating/boiling woodchips (no idea why i saved this but i figured it might have a use later so i froze it down)

Thats the stuff i have at hand, but if there are any usable things that are common in supermarkets and whatever please do let me know :takingnotes:

I was thinking of simply doing 50/50 grain water and tap water, 1 or few drops of karo per 1 liter and agar agar powder.
I am not sure about the ratios for agar agar though, if i got 1 liter of grain water with 1 drop of karo for example, i'd need how much agar? like 20 grams yeah? :stoned:

I just took a few too many hits i think, this turned into a long post :blazed:


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InvisibleMad Season
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Re: Help me choose my Agar recipe. [Re: Yerow]
    #22121405 - 08/21/15 09:13 AM (8 years, 5 months ago)

http://www.shroomery.org/5243/What-are-some-good-agar-media-recipes

http://www.shroomery.org/forums/showflat.php/Number/18431006
http://www.shroomery.org/forums/showflat.php/Number/19208976

Do as many as you can. Grain water will work too. The more you switch up recipes, the better the myc will perform. It doesn't like the same food source for too long.


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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AMU Q&A
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OfflineYerow
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Re: Help me choose my Agar recipe. [Re: Mad Season]
    #22121441 - 08/21/15 09:21 AM (8 years, 5 months ago)

Nice have not seen those two first links, thanks!


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Invisiblepaperbackwriter
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Re: Help me choose my Agar recipe. [Re: Yerow]
    #22121555 - 08/21/15 09:49 AM (8 years, 5 months ago)

50% grain water dilution has been far too nutritious for my likings.  I'll probably try 25% next time I make up a batch.

I don't add anything extra to it.


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley


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OfflineChancesAre
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Re: Help me choose my Agar recipe. [Re: paperbackwriter]
    #22121620 - 08/21/15 10:08 AM (8 years, 5 months ago)

:whathesaid:


--------------------
Did you see what God just did to us man?


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OfflineYerow
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Re: Help me choose my Agar recipe. [Re: ChancesAre]
    #22121670 - 08/21/15 10:23 AM (8 years, 5 months ago)

I will probably try PDYA first, since i've got those ingredients at hand, can get yeast just down the road.
And i am hungry, so no problem with boiling some potatoes :laugh:

Next batch i would like to try with grain water though, i have large amounts of that just sitting in my tiny freezer.

So no go to 50% dilution? Can try a little less i suppose, and no added sugars like karo, just water, grain water and agar? :thumbup:


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OfflineYerow
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Re: Help me choose my Agar recipe. [Re: Mad Season]
    #22121686 - 08/21/15 10:27 AM (8 years, 5 months ago)

Quote:

Mad Season said:
It doesn't like the same food source for too long.




What is too long? I have been using the same mix for probably half a year, i have not seen any changes in growth or the like.
Though looking through Franks agar recipes and pictures it's easy to see how the mycelium reacts to other nutes.


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InvisibleMad Season
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Re: Help me choose my Agar recipe. [Re: Yerow]
    #22121716 - 08/21/15 10:34 AM (8 years, 5 months ago)

It's not like it'll stop altogether, but it'll bring about senescence a lot quicker. Also it'll be harder for it to go into grains since it's so used to whatever agar recipe you used.

It's kinda like me giving you a burger every meal.. eventually you'd get sick of it. But you'd still be alive if I gave it to you. You can see how much stronger it is when you switch the nutrients often


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineYerow
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Re: Help me choose my Agar recipe. [Re: Mad Season]
    #22121728 - 08/21/15 10:38 AM (8 years, 5 months ago)

Huh alright, thanks for the info!
Looking forward to trying to throw my usual cube culture on PDYA, will also have fresh plates ready for the communal grow :rockon:


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Invisiblepaperbackwriter
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Re: Help me choose my Agar recipe. [Re: Yerow]
    #22121730 - 08/21/15 10:39 AM (8 years, 5 months ago)

Quote:

Yerow said:
So no go to 50% dilution? Can try a little less i suppose, and no added sugars like karo, just water, grain water and agar? :thumbup:




I would definitely do just grain water, water, and agar.  I can't say how strong your grain water is compared to mine.  We may use different ratios and I use oats and you use rye.

But I wouldn't start with anything higher than 50%.  It's also going to depend what you're doing.  Storing cultures more nutrition I assume would be better.  For strain isolation and out running contams I see very slow movement from my 50% dilutions.  The myc just has so much food it has little reason to seek out new food sources or create rhizomorphic growth (at least, that's my theory).


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley


Edited by paperbackwriter (08/21/15 10:41 AM)


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OfflineChancesAre
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Re: Help me choose my Agar recipe. [Re: paperbackwriter]
    #22122366 - 08/21/15 01:03 PM (8 years, 5 months ago)

Quote:

paperbackwriter said:
The myc just has so much food it has little reason to seek out new food sources or create rhizomorphic growth (at least, that's my theory).




Dead on sir! That's what I remember reading in Paul Stamets' book


--------------------
Did you see what God just did to us man?


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