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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
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Re: Mr. Fox's Tampanesis.. [Re: Teemo 6T3]
#22766194 - 01/11/16 02:43 PM (8 years, 20 days ago) |
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Tampanesis fruit clone on RGS

Slanted
Even made an LC 

Here's the cloned fruit body:
-------------------- Giving is all we have, for we're just grateful to be alive

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Psilosoulful
Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Beautiful
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
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Thankyou, Psilo!
-------------------- Giving is all we have, for we're just grateful to be alive

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myceliumEX
Philomathmycology



Registered: 12/10/15
Posts: 153
Loc: South East US
Last seen: 3 years, 10 months
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Inspiring Fanatic Mr. Fox! Looking forward to doing my own Tampanensis sometime.
Curious, what would happen if one were to clone from a sclerotia. I'm thinking myc is myc, so it would just grow and act like any other myc? Meaning cloning sclerotia won't get you a new sclerotia, just mycelium that that acts like any other?
Thanks for the post. Love it!
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B.C. by Johnny Hart "All mushrooms are edible, but some only once." -- Croatian Proverb
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
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Re: Mr. Fox's Tampanesis.. [Re: myceliumEX]
#22767304 - 01/11/16 07:17 PM (8 years, 19 days ago) |
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Thanks for all the kind words!
If you cut open the sclerotia and take a tissue sample then you would have successfully cloned the sclerotia, meaning any jar you inoculate with that culture will produce stones identical to the one you cloned. Same goes with fruits that have been cloned.
It's a good idea to keep a MS culture on hand though, just in case you want to work with new genetics
-------------------- Giving is all we have, for we're just grateful to be alive

Edited by Fantastic Mr. Fox (01/11/16 07:25 PM)
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bootster

Registered: 02/22/11
Posts: 1,531
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Re: Mr. Fox's Tampanesis.. [Re: Teemo 6T3]
#22767382 - 01/11/16 07:32 PM (8 years, 19 days ago) |
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Quote:
Teemo 6T3 said: So would sclerotia actually taste like shit if i spawn it to shit or coir?
I want to start spawning it, heard really good things of it being more potent and yields are horrendous.
I had a failed fruiting attempt and harvested the stones before I discarded the substrate. I didn't notice a change in flavor. Tasted like sheet before and after the exposure to the sub.
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Mr. Fox's Tampanesis.. [Re: bootster]
#22768750 - 01/12/16 04:23 AM (8 years, 19 days ago) |
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Maybe something is wrong with your taste buds 
I heard it has more of a nutty flavour..
Will be tasting mine soon though so we'll see..
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
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myceliumEX
Philomathmycology



Registered: 12/10/15
Posts: 153
Loc: South East US
Last seen: 3 years, 10 months
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Quote:
Fantastic Mr. Fox said: Thanks for all the kind words!
If you cut open the sclerotia and take a tissue sample then you would have successfully cloned the sclerotia, meaning any jar you inoculate with that culture will produce stones identical to the one you cloned. Same goes with fruits that have been cloned.
It's a good idea to keep a MS culture on hand though, just in case you want to work with new genetics
Re-reading my question, seems kinda dumb. Maybe naive is a better choice of words? Anyway, I was thinking maybe if you cloned the stone, could you then directly grow a stone. That's silly now that I re-read it though. Cloning a stone would get you more Tampanensis. derp.
Wait, you did say "that culture [produced from the huge stone clone] would produce identical stones to the stone that was cloned" So I was sort of on the right thought path... Does this mean that stone formation isn't random, depending on environmental factors, it's based on genetics?
Thinking out loud here, if you were to clone from a huge stone, (let's assume for the sake of argument, isolated down to the "huge stone" genetics if need be), then grow out the "huge stone strain", will all of the stones produced be the same huge stone of the original clone?
If that is the case, cloning/isolating out a huge stone strain seems like it could be a worthwhile project.
These Tampanensis grow so long and skinny, with tiny caps. Mush is such a neat organism.
Thanks Fantastic Mr. Fox! Fantastic is right.
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B.C. by Johnny Hart "All mushrooms are edible, but some only once." -- Croatian Proverb
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Mr. Fox's Tampanesis.. [Re: myceliumEX]
#22770473 - 01/12/16 02:45 PM (8 years, 19 days ago) |
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No, no your fine. No question is a dumb question.. That's how we learn! You'd be naive to not ask a question pertaining to what you don't know.
Anyway, environment and genetics play a role in stone formation. The culture won't produce stones unless under environmental stress & the genetics dictates the characteristics of the stones produced by thus said culture.
Stone cloning is very rewarding indeed! If you clone a huge stone from an MS inoculated jar, all the jars you start with the clone culture will produce stones just like the one you cloned. 
Idk how cult savvy you are, but if your going to be cloning giant stones, I'd look into making up a few bags, rather than jars.
&I don't see any need to isolate with stone producers, but cloning is paramount IMO
Idk if your interested in fruitbodies, but it's a good idea to have a strong stone clone && fruit clone when dealing with sclerotia producers.
Good luck, and feel free to ask me w/e I'll help however I can.
-------------------- Giving is all we have, for we're just grateful to be alive

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myceliumEX
Philomathmycology



Registered: 12/10/15
Posts: 153
Loc: South East US
Last seen: 3 years, 10 months
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Wow, that is awesome. Thanks for clearing things up, it's greatly appreciated.
I noc'd up my first jars ever this morning. Felt like a complete disaster at the time, but I'm feeling pretty good about it now. Screwed up a few things, but I think overall, my sterile techniques were solid. "My 1st rodeo" in mush cult if you get bored. Be prepared, there are a couple of monster posts from me.
Not very savvy with anything quite yet. I've been lurking/reading/learning for a long time. Sporeworks stocking stuffer special finally convinced me to stop researching, and start experimenting.
Love fruit bodies, and stones. All things mush really. Makes sense that you'd want both a strong fruiter, and stone producer. From what I've read from a few people that have proven themselves with the Mexicana family, (mostly stonesun, and a lot of good info from cervantes/rose too) These guys are usually either good fruiters, or good stone producers, not both. IIRC Tampanensis is "known" to be a potent, descent fruiter, with average to low numbers of smallish to medium stones (on average i'd think.) This thread is showing Tampanensis' fruiting capabilities.
I have a Jalisco syringe. It's known to be rather potent, a great fruiter, but crappy stone producer. If I'm understanding you correctly, depending on the skills of the cultivator, a great fruiter, and huge stone producer could potentially be isolated from any of the Mexicanas? Once I develop more skills, and get some experience, sounds like a perfect project.
Also makes sense what you said about stone formation, and the stressors needed to force the production of them. I forgot that stones are a long term survival strategy. According to stonesun, ATL/Gandolini, and Mexicana "A" iirc are the best fruiters/sclerotia maker combinations. I really, really want to do an ATL/Gandolini stealth/neglect grow.
Once I get my hands on some, planned to noc up a large jar (I saw a gallon mason jar at wallyworld :O to bad it won't fit in my PC), then "forget it" somewhere for around 6 months, harvest all of the sclerotia I can, then fruit the remaining spawn to bulk. Might try the same with this Jalisco I have sooner rather than later.
Does that sound like a half way plausible plan?
So much info, so little time.
Thanks so mush Mr Fox!
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B.C. by Johnny Hart "All mushrooms are edible, but some only once." -- Croatian Proverb
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: Mr. Fox's Tampanesis.. [Re: myceliumEX]
#22771815 - 01/12/16 07:33 PM (8 years, 18 days ago) |
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Hah, I was once a lurker myself, but we've all got to start somewhere. 
I only have experience with Galindoi, Tampenesis, Jalisco & Chicon nindo. Galindoi & Tampanesis both proved to be great fruiters && stone producers for me so far. For some reason my Chicon Nindo culture/jars aren't producing any stones what so ever. The mycelia has ambered over (turned orange) but no stones. lol Maybe it'll be a prolific fruiter  Either way I won't know until I move at the end of the month. Right now they're in ziplocks stored away in my refrigerator.
I do have Jalisco going from agar wedge & it immediately started forming stones from the wedge itself. I haven't fruited it yet though. (waiting until after the move)
Here's the Jalisco jar Im speaking of: (it's 100% now)

&& yes, any stone producer/mexicana can be a good fruiter and stone producer. All it takes is patience and perseverance.
Did you inoculate your jars with a syringe? I hope not... There's no guarantee that the syringe is 100% clean, so inoculating jars with it is in a way a crapshoot. You'll be wise to start your syringes on a 2D nutrient rich surface like agar-agar. That way you can isolate the clean myc from contaminates. Furthermore, I wouldn't start with gallon jars esp. with a syringe. If you decide to go with gallon jars only fill them half way, 3/4 max! Sterilize for a minimum of 2hrs and 30mins && inoculate with either LC, LI or G2G. Don't inoculate with spores or an agar wedge, it'll surely fail.
Hope that helps.
Don't rush through, take your time man.. you want to fully grasp each stage/process.
-------------------- Giving is all we have, for we're just grateful to be alive

Edited by Fantastic Mr. Fox (01/12/16 07:39 PM)
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fearnloathingg
Observer


Registered: 10/18/15
Posts: 21
Loc: Right There
Last seen: 7 years, 15 days
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Very inspiring Mr. Fox!
I started some Tampanesis Agar plates from ms a bit ago and im on the 5th transfer. about 2 weeks ago i was looking at an older plate from the 2nd transfer and i found one of the plates had a 1/2" long pin growing out of the culture. I transferred the small fruit body to a new plate and now am on my 2nd transfer from that clone. the grow rate from this sample is definitely out performing the other samples I have on plates that i transferred at the same time.
From what im reading in previous posts it sounds like this sample is promising as a fruiter. Looking forward to giving it a shot in the near future.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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What's the fruiting time like on tamps (sorry if this was already covered) I made a tray up with 2 pints tamps spawn to cvg, cased...well really just a thicker top layer...the stones destroyed the sub before I got any fruits...
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Q&A US vs. THEM The more I learn, the less I know.
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
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Which is why I'm solely against spawning the stones into the bulk substrate, unless you're after bigger stones. I'll spawn the small gravel-like stones, but never the massive ones because they're bound to bust shit up as they grow.
I get fruits within 8days usually, but it has taken up to 2 weeks (14days)
Quote:
fearnloathingg said: Very inspiring Mr. Fox!
I started some Tampanesis Agar plates from ms a bit ago and im on the 5th transfer. about 2 weeks ago i was looking at an older plate from the 2nd transfer and i found one of the plates had a 1/2" long pin growing out of the culture. I transferred the small fruit body to a new plate and now am on my 2nd transfer from that clone. the grow rate from this sample is definitely out performing the other samples I have on plates that i transferred at the same time.
From what im reading in previous posts it sounds like this sample is promising as a fruiter. Looking forward to giving it a shot in the near future.
Nice my friend! && Tnx Lately I've been hearing alot of occurrences where people are finding pins on their tampan plates. Good shit.
You should have yourself a nice fruiting culture there. ;)
-------------------- Giving is all we have, for we're just grateful to be alive

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myceliumEX
Philomathmycology



Registered: 12/10/15
Posts: 153
Loc: South East US
Last seen: 3 years, 10 months
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Quote:
Fantastic Mr. Fox said: Hah, I was once a lurker myself, but we've all got to start somewhere. 
I only have experience with Galindoi, Tampenesis, Jalisco & Chicon nindo. Galindoi & Tampanesis both proved to be great fruiters && stone producers for me so far. For some reason my Chicon Nindo culture/jars aren't producing any stones what so ever. The mycelia has ambered over (turned orange) but no stones. lol Maybe it'll be a prolific fruiter  Either way I won't know until I move at the end of the month. Right now they're in ziplocks stored away in my refrigerator.
I do have Jalisco going from agar wedge & it immediately started forming stones from the wedge itself. I haven't fruited it yet though. (waiting until after the move)
Here's the Jalisco jar Im speaking of: (it's 100% now)

&& yes, any stone producer/mexicana can be a good fruiter and stone producer. All it takes is patience and perseverance.
Did you inoculate your jars with a syringe? I hope not... There's no guarantee that the syringe is 100% clean, so inoculating jars with it is in a way a crapshoot. You'll be wise to start your syringes on a 2D nutrient rich surface like agar-agar. That way you can isolate the clean myc from contaminates. Furthermore, I wouldn't start with gallon jars esp. with a syringe. If you decide to go with gallon jars only fill them half way, 3/4 max! Sterilize for a minimum of 2hrs and 30mins && inoculate with either LC, LI or G2G. Don't inoculate with spores or an agar wedge, it'll surely fail.
Hope that helps.
Don't rush through, take your time man.. you want to fully grasp each stage/process.
I can think of worse things to be other than a lurker 
Never heard of Chico nindo, adding it to my list. I'd really like to do an outside grow of Tampanensis eventually. Interesting you got large stones from the Tampanensis. Was that after agar/isolation, or was that MS? Everything I've read about the Tampans says their stones are rather lackluster. Nice to hear otherwise. No way would I attempt to noc a gallon jar via MS.
I don't have any agar, nor any agar related tools/supplies yet. It's planned. Should be soon.
Yes, I noc'd all 5 Chitwan jars via MS syringe. I just couldn't wait. I know there are contam risks, and the genetics are a crapshoot at best. Imho, possibly some cubes are better than not even trying, and/or none. I can't wait to get home and see what I have happening after 24 hrs. I know there won't be much this soon yet. I noc'd up a quart jar w/about 2 - 2.5cc. A couple people said that was more than enough. Hoping that means the myc will go crazy. If I get at least 1 solid jar, I'll be happy, and will probably use it for a G2G if I don't have anything agar yet.
Question. I will be researching this as well, but is there anything I can substitute in place of agar? Not the process, l mean the actual agar medium. I think every tek I've seen requires at least some agar. If not, will just have to wait for the FUNds.
Is "ambering over" a precursor to stone production?
I'm very happy to hear your experience with Jalisco. Looking forward to using it even more now. 
I doubt I'll use a gallon jar. Especially as I don't think it'll fit in my 17qt Presto. Was more thinking/dreaming out loud. Plus those gallon jars are enormous!
Like I've hinted at, and said before, I want to do an outside grow of the Tampanensis. I figure i'll have a pretty good chance at success as I'm not all that far from where the only known wild fruit was found. I have my heart set on a stealth/neglect grow with the Gandoli/ATL for some reason.
I did read quite a bit about using MS. To help prevent contams from the needle, I squirted a tiny bit of solution out, then flamed the needle glowing red. Hope that does the trick. I'm pretty sure these luer-lock needles are PC'able right? Is it worth it? I know they are cheap to buy online, but they could be free if I'm waiting for them to ship ya know.
Definitely not rushing anything. I'm doing my best to learn everything I can. With people like you around here, sure makes things much more fun. I have a few cultivation/mush bio books to read in addition to these amazing forums.
Thank you so much for all your help/feedback/insight Mr Fox!
PS wrote this from my phone. Tried to catch typos and anything that doesn't make sense. The damn auto-correct doesn't know enough mycology yet, and makes some words a real PITA.
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B.C. by Johnny Hart "All mushrooms are edible, but some only once." -- Croatian Proverb
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myceliumEX
Philomathmycology



Registered: 12/10/15
Posts: 153
Loc: South East US
Last seen: 3 years, 10 months
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Re: Mr. Fox's Tampanesis.. [Re: myceliumEX]
#22773283 - 01/13/16 05:00 AM (8 years, 18 days ago) |
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Forgot one thing, that Jalisco jar is looking awesome. I hope my Chit jars are looking even a tiny bit like that Jalisco jar.
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B.C. by Johnny Hart "All mushrooms are edible, but some only once." -- Croatian Proverb
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Teemo 6T3
႟тнe мedιcιne мan ☼



Registered: 07/21/14
Posts: 1,570
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Re: Mr. Fox's Tampanesis.. [Re: myceliumEX]
#22773371 - 01/13/16 06:06 AM (8 years, 18 days ago) |
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I don't think there's any substitute for agar, we just use it to make a 2D surface, can't you buy online? it's really cheap..
If you can't buy online, you can find them in supermarkets/Asian stores, they come in packages like this... Sometimes in different packages but just ask the store employees.

And there are many teks for agar, i myself use Pasty's agar tek, very easy, and all the ingredient are found in any grocery shops...
As for other supplies, you don't really need them, you can substitute them with anything, you don't need to be all professional, like instead of petri dishes, i use normal food plastic dishes, that have a sign PP5 on them.
As for scalpels i actually use those normal extendible razor blades, hasn't failed me yet... 
-------------------- Shrooming Is Of The Essence   Rest In Peace Dankington
Edited by Teemo 6T3 (01/13/16 06:13 AM)
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bootster

Registered: 02/22/11
Posts: 1,531
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Re: Mr. Fox's Tampanesis.. [Re: myceliumEX]
#22773680 - 01/13/16 08:16 AM (8 years, 18 days ago) |
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myceliumEX said:
"Is "ambering over" a precursor to stone production?"
Sometimes you get something that looks like this:
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myceliumEX
Philomathmycology



Registered: 12/10/15
Posts: 153
Loc: South East US
Last seen: 3 years, 10 months
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Re: Mr. Fox's Tampanesis.. [Re: Teemo 6T3]
#22774109 - 01/13/16 11:01 AM (8 years, 18 days ago) |
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Quote:
bootster said: myceliumEX said:
"Is "ambering over" a precursor to stone production?"
Sometimes you get something that looks like this:

Thanks booster. I've seen that before in other posts/pics. I always thought it looked scary, and maybe even contaminated! Glad you showed me this, so if/when it happens to me I won't freak out So it's not necessarily related to stones, just myc. Does it actually mean anything when the myc does this?
Quote:
Teemo 6T3 said: I don't think there's any substitute for agar, we just use it to make a 2D surface, can't you buy online? it's really cheap..
If you can't buy online, you can find them in supermarkets/Asian stores, they come in packages like this... Sometimes in different packages but just ask the store employees.

And there are many teks for agar, i myself use Pasty's agar tek, very easy, and all the ingredient are found in any grocery shops...
As for other supplies, you don't really need them, you can substitute them with anything, you don't need to be all professional, like instead of petri dishes, i use normal food plastic dishes, that have a sign PP5 on them.
As for scalpels i actually use those normal extendible razor blades, hasn't failed me yet... 
I can order online, and I saw that it is super cheap. Just need the money, and time for it to get here once I order it. Damnit, I completely forgot that agar agar is available at the grocery store. Thanks for posting what the package looks like.
Point taken about using a container in lieu of a petri dish. You said you use "normal food plastic dishes, that have the sign PP5 on them" I've never seen PP5 on a dish, though I was never actually looking for it. Guess I'll see it soon enough. IIRC, pasty talks about the dishes to use in the pasty plate tek anyway.
So with a petri dish the lid is held in place with parafilm, which allows proper GE for the culture. Does a PP5 dish come with a lid that provides proper GE? I doubt it since it's primarily intended to hold food, not myc cultures I'm guessing I'd need to use GLAD wrap (saran wrap isn't made from the right compound) to close the dish?
Wait, not sure why I'm asking here polluting Mr Fox's awesome Tampanensis thread I'm sure the Pasty Plate tek goes into all those details. Thanks for pointers Teemo! I'll be agar agar'ing like a mofo soon enough. 
Maybe I'll study some of this Jalisco sooner rather than later once I get some pasty plates going.
I didn't mean to highjack your awesome Tampanensis grow thread Mr. Fox! Hopefully I'll have a Jalisco post with similar results before to long!
Thanks!
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B.C. by Johnny Hart "All mushrooms are edible, but some only once." -- Croatian Proverb
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