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InvisibleYami
Stranger
Registered: 08/18/15
Posts: 24
First grow attempt using dried stem on agar
    #22119348 - 08/20/15 11:02 PM (8 years, 5 months ago)

So heres a better picture of my canning jar "petri dish". This piece was taken from the inside of the stem of the mushrooms in the third photo. As you can see I did a few transers to other canning jars. Id also like to know if its time to transfer some of the agar from the first photo to a vermiculte and brf 1/2 pint jars, by cutting a few pieces out and putting them into the jars after pressure cooking?



These other jars were made the following day, using the same sterile techniques like cleaning up and using a Still air box and pressure cooker. I used a tyvek suit and mask and also have a sharp ion plasma cluster filter. Not a flow hood but I figured id use it.

1st picture is a old dried stem I threw into the agar uncleaned. 2nd picture is the inside of a stem dipped in peroxide before adding to agar. 3rd picture is also a stem dipped in peroxide before adding to agar. 4th picture is the inside of stem that didnt do much of anything. 5th picture I dont remember what I did there. 6th picture is also the inside of a stem that didnt do much of anything.



This was my first attempt at any of this, so I dont have the proper petri dishes and used canning jars. Ive read that its better to use smaller 1/4 sized jars and got some but this is what came from what I had. I also didnt want to spend to much money on things and fail miserably. I decided to try and grow them for myself, because its a pain in the ass having to ask everyone I meet, "hey, btw got any hook up on some shrooms"? Its a major part of my life and happiness. Id like to have em whenever I want! Thank you all for your help!


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OfflineICEMANOO9
Ssi


Registered: 06/25/12
Posts: 209
Last seen: 1 year, 6 days
Re: First grow attempt using dried stem on agar [Re: Yami]
    #22119480 - 08/20/15 11:41 PM (8 years, 5 months ago)

Yes under sterile conditions you want to scoop the agar wedge out and drop it Into a jar of (preferably) sterlized grains. Not brf. The myc will jump off the agar media onto the grains and once your jar is fully colonized you can transfer to other prepared sterlized grain jars thus multiplying your mycelium spawn


Edited by ICEMANOO9 (08/20/15 11:42 PM)


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InvisibleYami
Stranger

Registered: 08/18/15
Posts: 24
Re: First grow attempt using dried stem on agar [Re: ICEMANOO9]
    #22119599 - 08/21/15 12:20 AM (8 years, 5 months ago)

Thanks for your reply and very useful information. Ill check Franks WBS tek. Is that the way to go for what ive got going on?


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