Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   North Spore Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinebryanbzl
Spawn Runner
 User Gallery


Registered: 03/11/07
Posts: 563
Last seen: 5 years, 10 months
Alternatative tek for culture storage
    #22118502 - 08/20/15 08:36 PM (8 years, 5 months ago)

Not sure if someone has posted similar before, but this is how I've simplified my culture storage for my gourmet ad medicinals. I use the standard culture tube method but instead of using agar I use grain instead. The grain I use is hard red winter wheat. Here are some pics so you can get an idea.







The pros for using this method is what made me switch. firstly when using the standard agar method you need to cut or scoop (whatever way you do it) a piece of the culture from the tube and transfer to start a new plate for expansion. with this method I just open the culture tube, remove the polyfill wad, and remove a single grain to transfer to a new agar plate for expansion onto grain. I like this method because it allows a set uniform size of culture to be transferred to the agar dish and basically contains hundreds of separate grains which basically translate to plates.

Hope this helps simplify the process for someone or allows a creative view that inspires others to experiment. Any questions are more then welcomed.

BTW I start the cultures from standard agar wedges from either a stored tube or dish.


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


Extras: Filter Print Post Top
Offlineforrest
 User Gallery


Registered: 11/16/12
Posts: 1,011
Loc: The Netherlands
Last seen: 4 years, 6 months
Re: Alternatative tek for culture storage [Re: bryanbzl]
    #22119341 - 08/20/15 11:01 PM (8 years, 5 months ago)

i do a variation of that with little woodchips. I think with woodlovers they stay vital on it longer than on grain (mostly because it takes longer to digest and is less nutritious)


--------------------
My Trade List


Extras: Filter Print Post Top
OfflineOysterKing
His I ness
Male


Registered: 02/08/14
Posts: 237
Last seen: 11 months, 20 days
Re: Alternatative tek for culture storage [Re: forrest]
    #22119668 - 08/21/15 12:44 AM (8 years, 5 months ago)

:strokebeard:  :takingnotes:


--------------------
:grin:


Extras: Filter Print Post Top
OfflineHitsuzen
Space Cowboy
Male User Gallery

Folding@home Statistics
Registered: 10/17/08
Posts: 399
Loc: Earth Flag
Last seen: 3 years, 2 months
Re: Alternatative tek for culture storage [Re: OysterKing]
    #22119692 - 08/21/15 12:52 AM (8 years, 5 months ago)

That's pretty cool! How's the shelf life?


--------------------
Hate never yet dispelled hate,
Only love dispels hate.
This is the law,
Ancient and inexhaustible.
-Buddha


                           


Extras: Filter Print Post Top
Offlinenasyn
Stranger


Registered: 03/27/14
Posts: 86
Last seen: 2 years, 3 months
Re: Alternatative tek for culture storage [Re: forrest]
    #22120692 - 08/21/15 04:44 AM (8 years, 5 months ago)

Could probably shove a piece of stick in there with the grains and maybe get the best of both worlds. (easy to extract grain and long lasting wood)


--------------------
Trade List


Extras: Filter Print Post Top
Offlinebryanbzl
Spawn Runner
 User Gallery


Registered: 03/11/07
Posts: 563
Last seen: 5 years, 10 months
Re: Alternatative tek for culture storage [Re: nasyn]
    #22121402 - 08/21/15 09:11 AM (8 years, 5 months ago)

Quote:

nasyn said:
Could probably shove a piece of stick in there with the grains and maybe get the best of both worlds. (easy to extract grain and long lasting wood)




Well you all have a point. These tubes have been inoculated from traditional agar + popsicle stick culture tubes which I have as back up for proven long term storage. I haven't used these for longer then 2 years so I cannot vouch for it any longer. But I have used an agar petri for more then 5 years without any popsicle stick or any piece of wood. I think as long as you refrigerate and don't let your medium dry out, you'll be fine. Also there is half a popsicle stick in these grain tubes. The key is to transfer them into storage right as soon as they fully colonize. that way in cold storage they slow way down and have a long long time to continue metabolizing the grains. Also, hard red winter wheat are hard little grains - they take a while to eat them up fully.


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


Extras: Filter Print Post Top
Offlineforrest
 User Gallery


Registered: 11/16/12
Posts: 1,011
Loc: The Netherlands
Last seen: 4 years, 6 months
Re: Alternatative tek for culture storage [Re: bryanbzl]
    #22121412 - 08/21/15 09:15 AM (8 years, 5 months ago)

There's a difference between a culture staying alive or staying vigorous and young.

And i would recommend not first letting it fully colonise before putting in the fridge, with slants it's also the practice to just let the wedge you have put in recover and then refridgerate, so it still has space to run in the cold.


--------------------
My Trade List


Extras: Filter Print Post Top
Offlinebryanbzl
Spawn Runner
 User Gallery


Registered: 03/11/07
Posts: 563
Last seen: 5 years, 10 months
Re: Alternatative tek for culture storage [Re: forrest]
    #22121565 - 08/21/15 09:52 AM (8 years, 5 months ago)

That is exactly what I do. when I say fully colonized I am talking talking about the pre stage - when the first wispy growth is visible throughout the grain. Slants and agar are put into cold storage at about 80% for me. I leave a small rim around agar dishes. As per GGMM. I have never had a problem with any culture not being vigorous after that time. At least not anything worth complaining about. The true key is to store cultures that are young i.e. early division. The oyster you see in this thread will show you what I mean by "came back to life".

http://www.shroomery.org/forums/showflat.php/Number/22117129/fpart/2/vc/1#22117129


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


Extras: Filter Print Post Top
Offlinebryanbzl
Spawn Runner
 User Gallery


Registered: 03/11/07
Posts: 563
Last seen: 5 years, 10 months
Re: Alternatative tek for culture storage [Re: bryanbzl]
    #22121582 - 08/21/15 09:58 AM (8 years, 5 months ago)

However, I must state the importance of starting from spores and getting a new culture started every couple of years. Also, let me make it clear that as time passes in storage cultures very slowly start to loose vigour - that is a fact. With proper storage you can expect good results over a few years time.


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   North Spore Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* Isolate Storage r05c03 1,543 7 08/28/03 05:39 PM
by r05c03
* Maintaining clean viable cultures for mass distribution? Baby_Hitler 1,204 1 09/27/04 03:19 AM
by ragadinks
* Advice Needed For Sending Cultures Jackal 1,997 14 03/26/03 06:10 PM
by FocusHawaii
* Reishi tek hyper_dermic 9,693 12 06/27/20 07:47 AM
by Forrester
* Storage FocusHawaii 1,128 5 04/14/03 09:04 AM
by FocusHawaii
* Growing Gourmet Mushrooms Using a Cake Tek Oscuro_lobo 3,829 5 02/22/03 04:58 AM
by Richard_D_James
* Does the karo tek work for edibles/wood lovers? GnuBobo 959 2 11/20/04 06:33 PM
by deoxy187
* Honey water tek with gourmet mushrooms? Dmonikal 1,282 2 10/09/04 03:26 AM
by Dmonikal

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
1,157 topic views. 2 members, 12 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.008 seconds on 14 queries.