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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 5 months
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What do I google to get one of those fancy bottles yah got there?
I was just using an old full size 1/2 pint jar I got from when I bought premade jars.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Juiceh
Dabbing All Day
Registered: 09/25/12
Posts: 3,208
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Re: pour agar TEK [Re: taGyo] 1
#22085596 - 08/13/15 10:04 AM (8 years, 7 months ago) |
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Quote:
taGyo said: What do I google to get one of those fancy bottles yah got there?
I was just using an old full size 1/2 pint jar I got from when I bought premade jars.
"Pyrex media bottle"
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
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Re: pour agar TEK [Re: Juiceh] 1
#22085606 - 08/13/15 10:07 AM (8 years, 7 months ago) |
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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uninc4life2010
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Registered: 06/05/10
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Quote:
bodhisatta said:
Quote:
Pastywhyte said: Nice pics, good description
10-15 min is a little short of a PC run tho, for sure if your using grain soak water or the like I would up that.
for grain soak agar probably yes for most other agar recipes with amino acids and some sugars you don't want to cause maillard reactions and you also don't want to caramelize any sugars.
If it was going to caramelize, it would do so within those 15 minutes, so I would say that a longer PC time is just fine.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Considering even the toughest endospores get killed in 15m in liquid media I would say you're fine but do what works.
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eatyualive
Eat's You Alive :)
Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 5 months
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Quote:
Juiceh said:
Quote:
taGyo said: What do I google to get one of those fancy bottles yah got there?
I was just using an old full size 1/2 pint jar I got from when I bought premade jars.
"Pyrex media bottle"
Dude thank you,
They look so much easier to pour.
Quote:
eatyualive said:
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: pour agar TEK [Re: taGyo] 1
#22086379 - 08/13/15 11:35 AM (8 years, 7 months ago) |
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They have a special removable ring on the top of the threads. The pour ring is specifically to not make agar dribble Down the jar, it pours out like it should
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Juiceh
Dabbing All Day
Registered: 09/25/12
Posts: 3,208
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Re: pour agar TEK [Re: taGyo] 1
#22086516 - 08/13/15 12:03 PM (8 years, 7 months ago) |
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Quote:
bodhisatta said: They have a special removable ring on the top of the threads. The pour ring is specifically to not make agar dribble Down the jar, it pours out like it should
Yup! I love the lip ring feature! Makes the pouring a breeze with no drips at all! You don't have to give the bottle a twist at the end of the pour to prevent that last drip that you would get with another vessel. When I first read about the lip ring, I didn't think it would make such a difference. But after using it, wow! These bottles are totally worth it.
Here's a pic that shows the lip on the pyrex jar:
Quote:
taGyo said:
Quote:
Juiceh said:
Quote:
taGyo said: What do I google to get one of those fancy bottles yah got there?
I was just using an old full size 1/2 pint jar I got from when I bought premade jars.
"Pyrex media bottle"
Dude thank you,
They look so much easier to pour.
They are really easy to pour. I find the glass above the agar level feels cooler to the touch when pouring than the cuervo bottle I had been using. The cuervo bottle would drip after every pour unless I gave it a shake at the end of the pour to get that last drop onto the plate. I highly recommend the media bottles. Hell, I'm excited to get home tonight to pour some more plates with my new media bottles! I just received 11 cultures and I got some serious work to do. LoL!
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: pour agar TEK [Re: Juiceh] 1
#22086523 - 08/13/15 12:05 PM (8 years, 7 months ago) |
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Trek222
Trek222
Registered: 06/25/15
Posts: 67
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Hi Bodhisatta,
Thanks for sharing those lovely little Pyrex Media Bottles. I'd like to buy some. I was wondering if you could answer one question?
1. Did you drill holes in your lids and insert a synthetic filter inside like RR does in his video and then cover up your lid with foil before putting in the PC?
Once again thank you for sharing.
Peace
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: pour agar TEK [Re: Trek222] 1
#22098049 - 08/16/15 09:22 AM (8 years, 7 months ago) |
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i love pour agar
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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No I don't do a thing to the lid.
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Trek222
Trek222
Registered: 06/25/15
Posts: 67
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Thanks for the quick response Bodhisatta.
So I do not need a hole in the bottle cap of the pyrex borosilicate media storage bottle. Thank you.
This will be my first time doing agar. I'll be placing my bottles in a newly bought AA921
I'm curious how much (agar mix + volume of water) do you put in your 500ml bottles? I was under the assumption that I needed to have some headroom in the bottle - stated differently - the bottle should not be filled to the maximum capacity of 500 ml.
I was trying to get more information because I see that you, Pastywhite, Juiceh, and Stromrider are all using the same bottles, while most other people have chosen the liquor bottle alternative.
People who use liquor bottles usually place a synthetic filter or polyfill inside the lid to trap contaminants because of the vacuum created when the agar cools once the bottle is taken out of the PC.
Thanks for the clarification and for posting your Pour Agar Tek.
Edited by Trek222 (08/17/15 05:52 AM)
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: pour agar TEK [Re: Trek222] 1
#22101605 - 08/17/15 06:18 AM (8 years, 7 months ago) |
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I fill my bottle to 400. Which does 20 plates once you're good at pouring.
Some old hands do the filter lid. I used to. But I got a job in a lab and saw how everyone else but these mushroom guys did it. Since then I just used normal lids and my success rate has gone up. Not because of the lids but because of practice. The lid made no difference so why modify it.
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Trek222
Trek222
Registered: 06/25/15
Posts: 67
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That was a prompt reply
I'll follow your lead and fill the bottle to the 400ml mark and then practice pouring in the SAB until I get 20 plates.
You've cleared everything up for me.
Peace
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: pour agar TEK [Re: Trek222] 1
#22101703 - 08/17/15 07:09 AM (8 years, 7 months ago) |
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You can practice with a sleeve of dishes but you waste a sleeve since they won't be sterile anymore. It's maybe worth the practice if sourcing dishes isn't hard or expensive
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Trek222
Trek222
Registered: 06/25/15
Posts: 67
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A sleeve of 10 polystyrene dishes is $4.00 where I live. I've opted for a case of 500 (90mm x 15mm) dishes for approximately $70. I think the practice will definitely be worth it. Thanks for the suggestions.
Over and out.
Edited by Trek222 (08/17/15 07:48 AM)
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DirdyD
Registered: 12/01/14
Posts: 1,650
Last seen: 3 years, 2 months
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Re: pour agar TEK [Re: Trek222] 1
#22118215 - 08/20/15 07:21 PM (8 years, 7 months ago) |
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Thanks, B!
Quick question - what magnification range do you suggest on a microscope? Id like to buy one for use with agar to help insure clean samples and for fun. Ive been told Dino-lites work well.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: pour agar TEK [Re: DirdyD] 1
#22118299 - 08/20/15 07:46 PM (8 years, 7 months ago) |
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400x to see spores barely bacteria. Phase contrast at 400x helps for bacteria without stains. 1000x oil for seeing bacteria and detail in hyphae like my pics of clamp connections.
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