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OfflineCirclesAndStuff
Naútēs

Registered: 08/07/15
Posts: 20
Loc: Earth
Last seen: 8 years, 4 months
Possible Contam!?!?!
    #22117049 - 08/20/15 02:37 PM (8 years, 5 months ago)

So I was peeking in on my jars today and saw these two spots in one of them. This is my first grow so i'm not sure if its contam or spores. The growth is occurring right where i knocked them up. Also i'm only 4 days in and this is the only jar showing visible activity. Anyone care to venture a guess as to what it is?





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OfflineCirclesAndStuff
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Registered: 08/07/15
Posts: 20
Loc: Earth
Last seen: 8 years, 4 months
Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22117109 - 08/20/15 02:51 PM (8 years, 5 months ago)

Also how can i tell the difference between black mold and spores? I didn't remove the lid But best i can tell it still smells like its supposed to.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22117557 - 08/20/15 04:45 PM (8 years, 5 months ago)

Looks like growth radiating outward from a clump of spores. Post some more pics in a few days and I'll give you my updated opinion. I don't think you should stress too bad on this though. Start some new jars, just in case. It helps, mentally and productively, to have multiple projects going at once, just in case one doesn't pan out.

Mold doesn't always smell, especially not through the lid, which you shouldn't remove til 100 percent colonized.

If you have a properly constructed lid with a dry verm barrier, then you should remove the foil so they can better breathe.


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OfflineCirclesAndStuff
Naútēs


Registered: 08/07/15
Posts: 20
Loc: Earth
Last seen: 8 years, 4 months
Re: Possible Contam!?!?! [Re: TheEaglesGift]
    #22118707 - 08/20/15 09:17 PM (8 years, 5 months ago)

Will do. I don't have any micropore tape at the moment, and the aluminium foil is very loose fitting. Although i'm fairly sure it wouldn't hurt to remove the foil, i'm trying to avoid contams like the plague. This being my first grow i desperately need to be successful so i can acquire spore prints. I often have to remind myself i'm only 4-5 days in and that's why i'm not seeing growth yet. Ill Post some more pics monday-ish. Thanks for lending your expertise to a noob!


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22119185 - 08/20/15 10:13 PM (8 years, 5 months ago)

Quote:

. I often have to remind myself i'm only 4-5 days in and that's why i'm not seeing growth yet.



There IS growth around that clump of spores.

Do you have a dry verm barrier? If you do, you should take the foil off. Foil can inhibit gas exchange, which can encourage anaerobic bacteria growth and/or stalling of the cubensis mycelium. If you have a properly constructed lid/very barrier than that foil will do more harm than good, if you lid is a cluster fuck(I can't see what's under that foil), then the foil isn't going to really save you from contamination as it's not air tight or a proper gas exchange filter.


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OfflineCirclesAndStuff
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Registered: 08/07/15
Posts: 20
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Last seen: 8 years, 4 months
Re: Possible Contam!?!?! [Re: TheEaglesGift]
    #22119459 - 08/20/15 11:34 PM (8 years, 5 months ago)

Fair enough. I have removed the foil. My lids have 4 holes punched around the circumference. I substituted micro-pore tape for the breathable bandaid adhesive (what i had on hand). Although in hindsight it would seem that it should be applied after inoculation rather than before(noob). Thanks again for your advice. Ill post more pictures when i see some progression in mycelium growth.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22119716 - 08/21/15 12:59 AM (8 years, 5 months ago)

Is there a dry verm barrier?


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OfflineCirclesAndStuff
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Registered: 08/07/15
Posts: 20
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Last seen: 8 years, 4 months
Re: Possible Contam!?!?! [Re: TheEaglesGift]
    #22119975 - 08/21/15 01:42 AM (8 years, 5 months ago)

There is indeed.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22123017 - 08/21/15 03:18 PM (8 years, 5 months ago)

Then you don't even need tape, and you certainly don't need foil.

Make sure not to jostle your jars around too much, this will compromise the verm barrier if it shifts too much.


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OfflineCirclesAndStuff
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Registered: 08/07/15
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Re: Possible Contam!?!?! [Re: TheEaglesGift]
    #22124481 - 08/21/15 08:58 PM (8 years, 5 months ago)

Since yesterday i have found more growth. I now have 7/11 showing growth. I would definitely say the foil was stalling growth. I'm Not sure where i heard to do that. Thanks again for the support.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22124953 - 08/21/15 11:00 PM (8 years, 5 months ago)

My pleasure brother. Let me know if you have any questions going forward, I don't mind helping.

Best to ask here in this thread if the questions are related instead of a personal message, so other new cultivators can learn from it as well.


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OfflineCirclesAndStuff
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Registered: 08/07/15
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Re: Possible Contam!?!?! [Re: TheEaglesGift]
    #22140472 - 08/25/15 11:35 AM (8 years, 5 months ago)

Here's an update on growth. Everything seems to be coming along nicely. This is the most colonized jar but the rest are showing steady growth now that i have removed the foil.:wink: They were inoculated on or around 08/16/2015. I know colonization speed varies depending on multiple circumstances but they do appear healthy and are showing steady growth.





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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22141261 - 08/25/15 03:07 PM (8 years, 5 months ago)

They look great. Job well done, so far.


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Offlineconfuzzed
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Registered: 05/22/06
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Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22149564 - 08/26/15 11:53 PM (8 years, 5 months ago)

That "clump" is enough to inoculate 20 maybe even 50 jars.  :mypleasure:

Keep that in mind for next time. Maybe break the spores up for more "even" distribution to create a quicker colonizing time.

Those were some concentrated ass syringes  :jackiechanofapproval:

I have never seen a clump like that.  I would be shittin bricks if I got such clumps  :awedance:


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible Contam!?!?! [Re: confuzzed]
    #22149803 - 08/27/15 02:30 AM (8 years, 5 months ago)

Better to take those clumps to agar, which can knock up an infinite amount of jars with minimal effort.


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OfflineCirclesAndStuff
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Registered: 08/07/15
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Re: Possible Contam!?!?! [Re: TheEaglesGift]
    #22150209 - 08/27/15 07:59 AM (8 years, 5 months ago)

I absolutely would do that if i had been prepared to do that initially. This being my first grow am just hoping to get a decent amount of spore prints, so i can continue my endeavour. I do intend to purchase some agar dishes in the future. And I'm considering making grain masters in the future. I'm unclear on the size of the yield from these different techniques, and while i want to have a steady supply i don't want piles and piles of mushrooms laying around either.
FYI This is a B+ strain ordered from Sporeworks. The spore clump was just under the size of a dime and i shook it very well.


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OfflineCirclesAndStuff
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Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22150230 - 08/27/15 08:05 AM (8 years, 5 months ago)

I do have one jar that appears to be stalling. I'm not sure why, they were all prepared the same way and I checked to make sure the air holes were clear. Perhaps its growing inside the substrate I don't know. Sorry for the shoddy pics my actual camera is broken so i'm using my phone





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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22151886 - 08/27/15 02:55 PM (8 years, 5 months ago)

That jar may be stalled, sometimes we never figure out why it happened. Stalling can be caused by a few things, like too dry or too wet substrate, or bacterial contamination.

How are your temps?


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OfflineCirclesAndStuff
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Registered: 08/07/15
Posts: 20
Loc: Earth
Last seen: 8 years, 4 months
Re: Possible Contam!?!?! [Re: TheEaglesGift]
    #22151968 - 08/27/15 03:12 PM (8 years, 5 months ago)

They've Stayed around 70°F. There was a bit of blockage i cleared at the inoculation points so it might be a gas exchange issue. Ill wait 4-5 days and try flipping the jar. Its doesn't have enough growth to really worry about trying to isolate the colonized substrate. and its the only one stalling at this point so 10/11 isn't a big deal. Ill keep updating. (Not sure if this would be the proper thread)


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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Possible Contam!?!?! [Re: CirclesAndStuff]
    #22152324 - 08/27/15 04:21 PM (8 years, 5 months ago)

Don't flip the jar. Cultivators flip jars when they're stalling at almost full colonization. Flipping the jar can help when the bottom of a jar becomes compacted and the mycelium has difficulty colonizing the substrate there.

I can't really think of any way that flipping that jar would do any good.


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