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The Stretch Effect
Stranger

Registered: 08/12/15
Posts: 20
Last seen: 8 years, 4 months
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there is a weird border around my LC inoculation point? [pics]
#22116744 - 08/20/15 01:25 PM (8 years, 5 months ago) |
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is this trich? it sometimes looks kinda greenish but other times white? I could be psychosomatic... the border was much more distinct a few days ago. I made 2 LC's at the same time from the same source but only used one. I'm afraid my second one is also contaminated. will H2O2 kill it in LC I haven't used? if I give it enough time will my oyster mushrooms win the battle?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: there is a weird border around my LC inoculation point? [pics] [Re: The Stretch Effect]
#22116833 - 08/20/15 01:44 PM (8 years, 5 months ago) |
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Ok so first of all. That's fucked.
Secondly, do you use agar? I noticed you said 2 lcs from the same source, but you failed to specify if it was from a clean agar wedge , or anything else 
3rd, to properly test your LC, put a drop on some agar dishes. It'll tell you how clean it really is.
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The Stretch Effect
Stranger

Registered: 08/12/15
Posts: 20
Last seen: 8 years, 4 months
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Re: there is a weird border around my LC inoculation point? [pics] [Re: Mad Season]
#22117115 - 08/20/15 02:52 PM (8 years, 5 months ago) |
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I had never made a LC before and was curious, so took a piece of straw covered in myc out of my fruiting chamber and used that to inoculate the LC... in retrospect, not the best idea, but I found out its super easy to make a liquid culture! it seems to be a reoccurring theme of mine to learn everything the hard way. those mushrooms didn't fruit either, not enough perlite to keep the humidity up.
oh well... I'm learning, you only fail when you stop trying, right
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Mdahmer
Aloysius devadander abercrombie



Registered: 04/05/14
Posts: 2,516
Last seen: 6 years, 6 months
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Re: there is a weird border around my LC inoculation point? [pics] [Re: The Stretch Effect]
#22117142 - 08/20/15 02:59 PM (8 years, 5 months ago) |
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yes mold spores and other stuff land all over your colonized subs which can defend themselves...putting that into sugar water though
you should really only start LC with an agar wedge because even with the best spore prints you will have some bad stuff since fruits have been exposed to open air. you can always get lucky but best just to get into agar anyway cause its badass
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: there is a weird border around my LC inoculation point? [pics] [Re: Mdahmer]
#22117157 - 08/20/15 03:03 PM (8 years, 5 months ago) |
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agar is just as easy to do as LC. It's the exact same thing really. Whatever your recipe was, you can just add 2g of agar for every 100 ml.
Quote:
Mad Season said:
Quote:
hiperhiper said: so what's the right procedure with grains? I've checked the teks and nobody said anything about this. I didn't know I was not supposed to use syringe on grains. What is cleaning up syringe on agar? what does it mean?
http://www.shroomery.org/forums/showflat.php/Number/18431006 http://www.shroomery.org/forums/showflat.php/Number/18430998 http://www.shroomery.org/forums/showflat.php/Number/19208976 http://www.shroomery.org/forums/showflat.php/Number/21889950/page/1
All of those threads are amazing.
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Nature Boy
Stranger than most



Registered: 07/09/07
Posts: 8,241
Loc: Samsara
Last seen: 2 months, 6 days
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Re: there is a weird border around my LC inoculation point? [pics] [Re: Mad Season]
#22117174 - 08/20/15 03:08 PM (8 years, 5 months ago) |
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The first two threads are where I learned to do agar. Those threads were never done any better by anyone else IMHO. 
N.B.
-------------------- All submitted posts under this user name are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated bullshit. Note well: Sorry, but I do not answer PM's unless you are a long-time trusted friend. If you have a question, ask it in the appropriate thread.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: there is a weird border around my LC inoculation point? [pics] [Re: Nature Boy]
#22117179 - 08/20/15 03:10 PM (8 years, 5 months ago) |
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Ikr. Respect
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The Stretch Effect
Stranger

Registered: 08/12/15
Posts: 20
Last seen: 8 years, 4 months
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Re: there is a weird border around my LC inoculation point? [pics] [Re: Mad Season]
#22117538 - 08/20/15 04:39 PM (8 years, 5 months ago) |
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ive been playing with agar for about 2 months, lots of trial and error. I went to the store and bought some oyster mushrooms and did tissue transfers to PDA. there is a lot of really good info on this site, you guys are a wealth of knowledge. I will keep at it! thanks
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