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Offlinesuhbliem
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Registered: 07/01/15
Posts: 42
Last seen: 7 years, 4 months
question about flaming needle and sab
    #22114154 - 08/19/15 08:56 PM (8 years, 5 months ago)

I'm not sure when/where you are supposed to flame the needle when using a SAB.  should I flame the needle then put it in the sab then spray with lysol cover and let sit for a while or do I flame the needle inside the SAB after the lysol has been sprayed.  my only concern is the alcohol content in the spray may ignite.


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OfflineCrimsonSpectre
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Re: question about flaming needle and sab [Re: suhbliem]
    #22114214 - 08/19/15 09:09 PM (8 years, 5 months ago)

:facepalm3:


--------------------
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OfflinetaGyo
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Re: question about flaming needle and sab [Re: CrimsonSpectre]
    #22114231 - 08/19/15 09:12 PM (8 years, 5 months ago)

First set up your SAB. Spray alcohol into it or whatever you use (USE 70% ISO NOT 90%) and wipe it down real good. Then leave it be for 30 minutes and go get ready by washing your hands, putting on gloves, sanitizing your hands, etc.

Flame it outside, put it in the SAB.

The reason why this is okay is because while the needle is hot a phenomenon called thermoporesis is happening. The heat coming off the needle repels all airborne contams.

Don't spray or wipe the needle with alcohol.

Quote:

taGyo said:
Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation.
Don't wipe your needle with alcohol after flame sterilization:

Quote:

swatt_haze said:
wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.





Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.

Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar.
Grains is 1CC as well.

After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.

If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.




Quote:

CrimsonSpectre said:
:facepalm3:



We're not all great growers like you.

Give the guy a chance :wink:


--------------------
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OfflinePsilosopherr
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Re: question about flaming needle and sab [Re: taGyo]
    #22114262 - 08/19/15 09:21 PM (8 years, 5 months ago)

see "how frank gets shit done" which is linked in my sig for a great write-up on how to use a SAB.

Personally I just have a propane torch on a table near my SAB. I have it lit the entire time I'm doing sterile procedures. This avoids the occasional issues that arise from using lighters with gloves on, and makes flaming nice and quick. (and like that guy said, don't wipe after flaming)

Although having a lit torch in the same room as a bottle of iso alcohol is pretty sketchy. I still can't decide if its too dangerous to be worth it or not.


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Invisiblewowimflabbergasted
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Re: question about flaming needle and sab [Re: taGyo]
    #22114764 - 08/19/15 11:29 PM (8 years, 5 months ago)

Quote:

taGyo said:
First set up your SAB. Spray alcohol into it or whatever you use (USE 70% ISO NOT 90%) and wipe it down real good. Then leave it be for 30 minutes and go get ready by washing your hands, putting on gloves, sanitizing your hands, etc.

Flame it outside, put it in the SAB.

The reason why this is okay is because while the needle is hot a phenomenon called thermoporesis is happening. The heat coming off the needle repels all airborne contams.

Don't spray or wipe the needle with alcohol.

Quote:

taGyo said:
Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation.
Don't wipe your needle with alcohol after flame sterilization:

Quote:

swatt_haze said:
wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.





Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.

Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar.
Grains is 1CC as well.

After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.

If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.




Quote:

CrimsonSpectre said:
:facepalm3:



We're not all great growers like you.

Give the guy a chance :wink:



Don't listen to this guy. There isn't enough alcohol in Lysol to ignite vapors. Inside your SAB is fine.

:trololol:


--------------------
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OfflinetaGyo
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Re: question about flaming needle and sab [Re: wowimflabbergasted]
    #22114769 - 08/19/15 11:30 PM (8 years, 5 months ago)

Omg he said lysol :facepalm:


--------------------
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OfflinePsilosoulful

Registered: 09/05/14
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Re: question about flaming needle and sab [Re: taGyo]
    #22114782 - 08/19/15 11:31 PM (8 years, 5 months ago)

:laugh2:


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OfflinetaGyo
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Re: question about flaming needle and sab [Re: Psilosoulful]
    #22114787 - 08/19/15 11:32 PM (8 years, 5 months ago)

Wait Flab, do you flame your needle inside your SAB?

Do you have one of those tall ones? 'Cause I got the long fat ones.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Invisiblewowimflabbergasted
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Re: question about flaming needle and sab [Re: taGyo]
    #22114798 - 08/19/15 11:34 PM (8 years, 5 months ago)

Yeah man I make sure to spray aaalloooottttt of lysol in there too right beforehand. I find it really keeps the needle nice and sterilized with the extra flames and all.


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OfflinetaGyo
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Re: question about flaming needle and sab [Re: wowimflabbergasted]
    #22114802 - 08/19/15 11:35 PM (8 years, 5 months ago)

:congrats::laugh2:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

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Invisible36fuckin5
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Re: question about flaming needle and sab [Re: wowimflabbergasted]
    #22114825 - 08/19/15 11:45 PM (8 years, 5 months ago)

Quote:

wowimflabbergasted said:
Give the guy a chance :wink:



Don't listen to this guy. There isn't enough alcohol in Lysol to ignite vapors. Inside your SAB is fine.




I know you're bullshitting to be funny, but the thousands of people who read this forum every day and don't sign up don't know the difference.

NEVER spray anything flammable into your SAB. Concentrating flammable fumes in your house is never a good idea, be it making BHO or alcohol in a SAB. Soapy water is all you need in your SAB. Materials that can't be flamed (jars, petris, etc) get wiped with alcohol. Metal tools get flamed outside the box.

rbalzer, of all people, was the only one to provide an answer.


--------------------
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If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflinecronicrFacebook
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Re: question about flaming needle and sab [Re: 36fuckin5]
    #22114878 - 08/19/15 11:57 PM (8 years, 5 months ago)

flames=heat currents flame outside the sab no matter what ya use


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I'm tired do me a favor


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InvisibleInocuole
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Re: question about flaming needle and sab [Re: 36fuckin5]
    #22114892 - 08/20/15 12:01 AM (8 years, 5 months ago)

Quote:

36fuckin5 said:
Quote:

wowimflabbergasted said:
Don't listen to this guy. There isn't enough alcohol in Lysol to ignite vapors. Inside your SAB is fine.




I know you're bullshitting to be funny, but the thousands of people who read this forum every day and don't sign up don't know the difference.

NEVER spray anything flammable into your SAB. Concentrating flammable fumes in your house is never a good idea, be it making BHO or alcohol in a SAB. Soapy water is all you need in your SAB. Materials that can't be flamed (jars, petris, etc) get wiped with alcohol. Metal tools get flamed outside the box.

rbalzer, of all people, was the only one to provide an answer.




Holy shit thanks for saving me the time here, what a turnout... :facepalm:

Like 36 said, try not to build up fumes, spray the inside with water, soapy or not, doesn't matter much, and flame outside the SAB and get it in before the tip stops glowing, but very carefully and smoothly.


--------------------
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Coir-ca-cola
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Invisiblewowimflabbergasted
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Re: question about flaming needle and sab [Re: Inocuole]
    #22114903 - 08/20/15 12:04 AM (8 years, 5 months ago)

If someone is dumb enough to light a flame in a box filled with combustible vapors, then they deserve whatever they get. Lesson learned the hard way I suppose...that's the only way for some people.

Seriously, that's like my coffee cup lid warning me my coffee's hot.


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OfflinecronicrFacebook
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Re: question about flaming needle and sab [Re: wowimflabbergasted]
    #22114915 - 08/20/15 12:08 AM (8 years, 5 months ago)

they should start putting this on syringes"for microscopy only, do not stab yourself in  the neck"


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisiblewowimflabbergasted
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Re: question about flaming needle and sab [Re: cronicr]
    #22114919 - 08/20/15 12:10 AM (8 years, 5 months ago)

No shit lol. "Not intended for intravenous drugs".


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InvisibleInocuole
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Re: question about flaming needle and sab [Re: wowimflabbergasted]
    #22114938 - 08/20/15 12:12 AM (8 years, 5 months ago)

Quote:

wowimflabbergasted said:
If someone is dumb enough to light a flame in a box filled with combustible vapors, then they deserve whatever they get. Lesson learned the hard way I suppose...that's the only way for some people.

Seriously, that's like my coffee cup lid warning me my coffee's hot.




People talk shit on this all the time but that lady got 3rd degree burns and had to get skin grafts and reconstructive surgery on her vag.  Companies are putting that warning there so they can get away with selling way-too-hot coffee, not because people are so dumb they don't understand coffee is hot.  Okay so a few people obviously are but nobody in charge of a company cares about those people.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflinecronicrFacebook
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Re: question about flaming needle and sab [Re: Inocuole]
    #22114941 - 08/20/15 12:13 AM (8 years, 5 months ago)

were any of you around when that dude blew his sab up a couple years ago? EPIC


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleInocuole
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Re: question about flaming needle and sab [Re: cronicr]
    #22114954 - 08/20/15 12:16 AM (8 years, 5 months ago)

Quote:

cronicr said:
were any of you around when that dude blew his sab up a couple years ago? EPIC




I might've been... I dunno, I hear about people burning the hair off their arms and eyebrows so many times here it's lost the funniness.

You can laugh but some people really are that dumb.  And maybe they deserve a face full of fire but if they're living with a toddler that actually has a future and that kid can't escape from a potentially big ass fire, then that's soooort of on you.  I wouldn't wanna live with that.  :shrug:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflinePussyFart
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Re: question about flaming needle and sab [Re: cronicr]
    #22114956 - 08/20/15 12:17 AM (8 years, 5 months ago)

Quote:

cronicr said:
were any of you around when that dude blew his sab up a couple years ago? EPIC



:loveintheair: I was....I remember that.....


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