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OfflineThe Stretch Effect
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Sanity Check!?
    #22111820 - 08/19/15 01:07 PM (8 years, 5 months ago)

hey errrbuudyy!!

This is my first time working with agar, I inoculated my plates 3 days go with one drop per plate out of a barren looking syringe (and yes, I know what microscopic means... and yes, I did shake it). I'm getting about half my plates where it seems only one spore has germinated... Dont I need at least 2 spores to germinate per plate to get viable fruiting mycelium? I know its early but what if only one spore germinates on the plate? can I transfer two single germinated mycelium together on a fresh plate to get fruiting mycelium (roll the dice and hope they are "compatible") or do I cut my losses? or do have that all wrong in my head??? last question, this might be for the strain thread, does a faster germination time correlate with positive overall growth rate or health of the mycelium/fruit bodies?

thanx guysch


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OfflineDeTwizzle
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Re: Sanity Check!? [Re: The Stretch Effect]
    #22111934 - 08/19/15 01:23 PM (8 years, 5 months ago)

I think you probably need to read some more. Sanity check? Perhaps?
There is no need to combine two plates of agar to get growth...???

Where do we send him... off to the RR Videos?
http://www.shroomery.org/forums/showflat.php/Number/21032261

Sounds like you need a better understanding of the basics.


Edited by DeTwizzle (08/19/15 01:24 PM)


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OfflineThe Stretch Effect
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Re: Sanity Check!? [Re: DeTwizzle]
    #22111971 - 08/19/15 01:30 PM (8 years, 5 months ago)

"A typical single spore germinates into a homokaryotic mycelium, which cannot reproduce sexually; when two compatible homokaryotic mycelia join and form a dikaryotic mycelium; that mycelium may form fruiting bodies such as mushrooms."

per wiki


spores grow just fine, but wont fruit until they become dikaryotic


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OfflineDeTwizzle
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Re: Sanity Check!? [Re: The Stretch Effect]
    #22111979 - 08/19/15 01:33 PM (8 years, 5 months ago)

Okay... I am guessing that you or whomever made it(the syringe)put more than one spore into it.

Also it takes a week or two for an agar plate to grow out.

Here, read this if you haven't.
http://www.shroomery.org/forums/showflat.php?Number=21176491&page=0

Also, here is how it sould & shouldn't look, it has agar dishes if you scroll down.
http://www.shroomery.org/forums/showflat.php/Number/17231150#17231150


Edited by DeTwizzle (08/19/15 01:36 PM)


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OfflineThe Stretch Effect
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Re: Sanity Check!? [Re: DeTwizzle]
    #22112004 - 08/19/15 01:39 PM (8 years, 5 months ago)

yea, I have 45 plates,
5 of which have 2 places where mycelium is growing independently of each other. 20 of which where there is growth out of one place.
20 which have no growth.

I would attach pics but my phone doesn't like the macro setting and everything is blurry as shit, and I'm looking for the charger for my real camera

I guess my question is, does homokaryotic mycelium look different from dikaryotic mycelium?


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OfflineDeTwizzle
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Re: Sanity Check!? [Re: The Stretch Effect]
    #22112108 - 08/19/15 02:02 PM (8 years, 5 months ago)

Homokaryotic myc is probably invisible to the human eye. It is the myc of a single spore. This would only happen if you put a single spore onto agar. Something like that would be very hard to do with out the right equipment. Once two or more spores connect then you have dikaryotic myc...and you are visually seeing dikaryotic myc on your plates, not mono...

Start here...
http://www.shroomery.org/forums/showflat.php/Number/19140341#19140341


Edited by DeTwizzle (08/19/15 02:03 PM)


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OfflineGreenRabbit
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Re: Sanity Check!? [Re: The Stretch Effect]
    #22112268 - 08/19/15 02:35 PM (8 years, 5 months ago)

When you drop from a syringe to agar, rock the plate around to distribute spores and distance contamination.

You probably have hundreds of spores growing from anywhere you see growth.


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OfflineThe Stretch Effect
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Re: Sanity Check!? [Re: GreenRabbit]
    #22113022 - 08/19/15 05:01 PM (8 years, 5 months ago)

these are really blurry but this is the best I could do with my phone



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OfflineDeTwizzle
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Re: Sanity Check!? [Re: The Stretch Effect]
    #22113456 - 08/19/15 06:33 PM (8 years, 5 months ago)

yep looks like ms syringe to agar. should continue to grow. Read up on transferring and sectoring... the linkz where provided.


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InvisibleGrey
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Re: Sanity Check!? [Re: GreenRabbit]
    #22113618 - 08/19/15 07:05 PM (8 years, 5 months ago)

Quote:

GreenRabbit said:
When you drop from a syringe to agar, rock the plate around to distribute spores and distance contamination.

You probably have hundreds of spores growing from anywhere you see growth.






Rocking the liquid around the plate won't distance contams. It'll just spread them around.


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OfflineKizzle
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Re: Sanity Check!? [Re: DeTwizzle]
    #22113774 - 08/19/15 07:38 PM (8 years, 5 months ago)

Quote:

DeTwizzle said:
Homokaryotic myc is probably invisible to the human eye. It is the myc of a single spore. This would only happen if you put a single spore onto agar. Something like that would be very hard to do with out the right equipment. Once two or more spores connect then you have dikaryotic myc...and you are visually seeing dikaryotic myc on your plates, not mono...

Start here...
http://www.shroomery.org/forums/showflat.php/Number/19140341#19140341



It's not hard a all. You streak spores onto a plate and further down the streak you go the fewer spores that are deposited. Eventually you'll find single spore colonies.

If you have a microscope you can easily tell the difference. Without one there's some uncertainty but monokaryotic colonies are the first to appear on a spore plate. They will either form a gap between the colonies where they or intersect or grow together into a single colony and new dikartoic mycelium will be seen growing outward from that colony. As for the general appearance of the monokaryons I can't think of any distinguishing feature other than I don't believe monokaryotic colonies ever produce highly rhizomorphic growth and the mycelium doesn't seem to get very thick.


At three o'clock there you can see a single monokaryotic colony whereas the other have already started combining.


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InvisibleInocuole
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Re: Sanity Check!? [Re: Kizzle]
    #22113846 - 08/19/15 07:57 PM (8 years, 5 months ago)

Kizzle and DeTwizzle!  Classic.


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OfflineDeTwizzle
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Re: Sanity Check!? [Re: Inocuole]
    #22113921 - 08/19/15 08:12 PM (8 years, 5 months ago)

Quote:

Inocuole said:
Kizzle and DeTwizzle!  Classic.



Well rate me! Muchswzan gave me a bad rating b/c of some dicknose comment I made?!


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InvisibleInocuole
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Re: Sanity Check!? [Re: DeTwizzle]
    #22113947 - 08/19/15 08:18 PM (8 years, 5 months ago)

You could always take that up with Munch and he might remove it if you're nice about it.  He doesn't strike me as the kind of dude to hold a grudge if you don't make as many dicknose comments anymore. :shrug:  In fact that solution will get you a much higher average than if I tried to cover his up with a 5.

I'm not gonna rate you just because your name rhymes with Kizzle's though.  Keep posting.  For the record I wouldn't have rated you negatively for those comments, but there was no need to make so many of them to the same person.

I will rate Kizzle though. :lol:


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OfflinePrimalSoup
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Re: Sanity Check!? [Re: The Stretch Effect]
    #22113957 - 08/19/15 08:21 PM (8 years, 5 months ago)

Quote:

The Stretch Effect said:
I know its early but what if only one spore germinates on the plate?




The odds are about 1,000,000:1 against that.  The only time I've had such sparse germination was after first rehydrating a 15 year old Ps. cyanescens refrigerated print in distilled water for about a month, and I got only a few germinations.  Anything ordinary and you'll have endless amounts - that's why it takes around 10-12 transfers to isolate from a MS innoc...

That said, some spore or another is always the first to germinate, and they generally take their own sweet time to do it.  So just watch and wait. :thumbup:


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OfflineDeTwizzle
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Re: Sanity Check!? [Re: Inocuole]
    #22113972 - 08/19/15 08:24 PM (8 years, 5 months ago)

Quote:

Inocuole said:
You could always take that up with Munch and he might remove it if you're nice about it.  He doesn't strike me as the kind of dude to hold a grudge if you don't make as many dicknose comments anymore. :shrug:  In fact that solution will get you a much higher average than if I tried to cover his up with a 5.

I'm not gonna rate you just because your name rhymes with Kizzle's though.  Keep posting.  For the record I wouldn't have rated you negatively for those comments, but there was no need to make so many of them to the same person.

I will rate Kizzle though. :lol:




He actually did it. I have much respect for him since I sense that he has contributed more than most to this site! Thx Munch!!!


Edited by DeTwizzle (08/19/15 08:25 PM)


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InvisibleInocuole
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Re: Sanity Check!? [Re: DeTwizzle]
    #22113976 - 08/19/15 08:25 PM (8 years, 5 months ago)

Told ya.  Was probably just having a shitty day or something.  Now you've got 5.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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