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Offlinethebug76
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Contamination or normal? Pic
    #22110907 - 08/19/15 11:22 AM (8 years, 5 months ago)

I'm getting a yellowing on the bottom of one of my pf jars. This is the first time this has happened in 3 batches, so it looks odd to me, but like I said, only 3 batches (36 jars). Is this a contaminate.


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Invisiblemrbart4444
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Registered: 09/13/14
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Re: Contamination or normal? Pic [Re: thebug76]
    #22110924 - 08/19/15 11:25 AM (8 years, 5 months ago)

Quote:

thebug76 said:
I'm getting a yellowing on the bottom of one of my pf jars. This is the first time this has happened in 3 batches, so it looks odd to me, but like I said, only 3 batches (36 jars). Is this a contaminate.





Its not about how many jars you do its how clean you do it. That looks normal to me though but if the jars have a garbage smell to them i wouldnt trust it.


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OfflinePinPornProducer
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Re: Contamination or normal? Pic [Re: thebug76]
    #22110934 - 08/19/15 11:28 AM (8 years, 5 months ago)

Looks like metabolites to me, the myc looks healthy. Worst case scenario is its a little bacteria which shouldn't cause any real issues


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Offlinethebug76
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Re: Contamination or normal? Pic [Re: mrbart4444]
    #22110980 - 08/19/15 11:32 AM (8 years, 5 months ago)

Thanks mrbart4444, I use a SAB and I wear gloves and clean everything with bleach and alcohol just like all the teks suggest. I'm new to the hobby, but I feel confident on my cleanliness. I'm not one to generally cut corners anyway. I just wanted an experienced eye to check it out.


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Offlinewolf8312
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Re: Contamination or normal? Pic [Re: thebug76]
    #22111187 - 08/19/15 12:04 PM (8 years, 5 months ago)

Tough call, but I dont think it looks too good myself. Too many metabolites showing all over the place and bottom left the myc has that bacterial gungy or smeared look.

Might be wrong of course!

Last grow I was certain I had bacteria from the look of my jars but when I fruited them I had success didnt smell anything bad either.

Tubs performed badly though and I still feel that they were in fact contaminated with bacteria as the texture of the mycelium just looked off.

Hard to explain maybe like I said kind of smeared. Dont throw them out though, I could be wrong, just gonna throw in my chips on black and watch the wheel go round! 

Let us know.


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"I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of."

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InvisibleMad Season
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Re: Contamination or normal? Pic [Re: wolf8312]
    #22111201 - 08/19/15 12:07 PM (8 years, 5 months ago)

You mean like this wolf :lol: I had the same results:
Quote:

Mad Season said:
It looks bacterial IMO.

http://www.shroomery.org/forums/showflat.php/Number/21890806#21890806 bacteria in spawn. You can also get it from prepping the substrate too wet. If you read in the link it explains what happened when I spawned bacterial spawn.




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Offlineshroomyaxn
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Re: Contamination or normal? Pic [Re: thebug76]
    #22111274 - 08/19/15 12:32 PM (8 years, 5 months ago)

Quote:

thebug76 said:
Thanks mrbart4444, I use a SAB and I wear gloves and clean everything with bleach and alcohol just like all the teks suggest.




You aren't using those rubber gloves for cleaning, are you?
Use those disposable latex gloves for one time uses only. 

Also, I would recommend wearing tyvek sleeves to cover the wrists and forearms, if you are not already.  I re-use the sleeves just a few times and always store them in a zip lock bag to keep the dust off them.


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Offlinethebug76
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Re: Contamination or normal? Pic [Re: Mad Season]
    #22111311 - 08/19/15 12:35 PM (8 years, 5 months ago)

The bottom left is where its not fully colonized I think, that side of the jar hasn't caught up yet. The strain is PE6 and I only inoculated one hole, so they're colonizing slow.

All 12 jars have some condensation on the inside, every jar I've done has, but I figured that was because it's warmer inside of the jar than the outside.


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Offlinethebug76
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Re: Contamination or normal? Pic [Re: thebug76]
    #22111342 - 08/19/15 12:36 PM (8 years, 5 months ago)

Shroomyaxn  they're  disposable, one time use only.


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InvisibleMad Season
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Re: Contamination or normal? Pic [Re: thebug76]
    #22111376 - 08/19/15 12:38 PM (8 years, 5 months ago)

I didn't say condensation. Every jar does condensate. I said wet/mushy grains stuck to the side of the jar in that post.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinewolf8312
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Re: Contamination or normal? Pic [Re: Mad Season]
    #22111432 - 08/19/15 12:43 PM (8 years, 5 months ago)

Quote:

Mad Season said:
You mean like this wolf :lol: I had the same results:
Quote:

Mad Season said:
It looks bacterial IMO.

http://www.shroomery.org/forums/showflat.php/Number/21890806#21890806 bacteria in spawn. You can also get it from prepping the substrate too wet. If you read in the link it explains what happened when I spawned bacterial spawn.








Lol! Looking at that 'completely clean' spawn now I dont think I've ever not had bacteria!

Yeah some of those pictures were like some of mine -not quite that blatent though- the white seems too waxy and solid, too pushed up against the glass with speckled spots of grain everywhere that for some reason havent colonized properly.



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Offlinethebug76
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Re: Contamination or normal? Pic [Re: Mad Season]
    #22111576 - 08/19/15 12:51 PM (8 years, 5 months ago)

Mad Season  I understand that. I was just talking about the moisture in general.

Like I said tho, they're not fully colonized, probably 70%-75%. They should be done in a few days going by my previous batches. I'll post back with more pics then.

I appreciate all the help I'm getting from everyone.


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InvisibleMad Season
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Re: Contamination or normal? Pic [Re: thebug76]
    #22111772 - 08/19/15 01:04 PM (8 years, 5 months ago)

In general condensation is fine. Hell metabolites can be fine. Metabolites are just metabolizing and digesting the substrate. In general though, metabolites and bruising before full colonization isn't a good sign, and can really lower your BE.

@ wolf, it's still a heated debate today that getting jars to be 100% clean of bacteria is impossible. However increasing pc times generally makes them much cleaner looking


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinethebug76
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Re: Contamination or normal? Pic [Re: Mad Season]
    #22112048 - 08/19/15 01:48 PM (8 years, 5 months ago)

In response to the glove statement, is vinyl gloves fine? The only latex ones my local wally world has is one size fits all (which is a lie, they tear everytime I try to put one on), the only ones I can find in xlarge are vinyl.


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InvisibleMad Season
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Re: Contamination or normal? Pic [Re: thebug76]
    #22112088 - 08/19/15 01:56 PM (8 years, 5 months ago)

Between you and me, in a still air box I don't use gloves or arm sleeves since making that tek. I just wash my hands and arms in soap and water. I work in a fairly still air room in a box with big 6" arm holes. I lit an incense in my sab, and used the smoke to see where there was lots of moving air. I found it happening most on the arm holes as you move your arms in and out. Increasing the size of the holes made it still af inside. It made the pressure a lot more equal.

The main point of a sab is to NEVER let anything hover over open media. In my tek I show how I never let that happen. Thus I can go straight bare handed. I haven't noticed any differences in contams since I've stopped using gloves:shrug:. You've just gotta be extremely careful and thorough. It took lots of practice for me to be confident in my sab work.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinethebug76
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Re: Contamination or normal? Pic [Re: Mad Season]
    #22112677 - 08/19/15 03:53 PM (8 years, 5 months ago)

I think I'll keep using gloves and wrapping my arms anyway. I have to wear gloves at work, so I'm used to it. Plus, my house is really old and has mold all over the place, so it's probably for the best.


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Offlineshroomyaxn
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Re: Contamination or normal? Pic [Re: Mad Season]
    #22122984 - 08/21/15 03:09 PM (8 years, 5 months ago)

Quote:

Mad Season said:
Between you and me, in a still air box I don't use gloves or arm sleeves since making that tek. I just wash my hands and arms in soap and water. I work in a fairly still air room in a box with big 6" arm holes. I lit an incense in my sab, and used the smoke to see where there was lots of moving air. I found it happening most on the arm holes as you move your arms in and out. Increasing the size of the holes made it still af inside. It made the pressure a lot more equal.

The main point of a sab is to NEVER let anything hover over open media. In my tek I show how I never let that happen. Thus I can go straight bare handed. I haven't noticed any differences in contams since I've stopped using gloves:shrug:. You've just gotta be extremely careful and thorough. It took lots of practice for me to be confident in my sab work.




Thanks for the tips.:thumbup:  I work in a small room and have 5" inch arm holes.  I agree, hovering is the main threat. 

I might cut back on the tyvek sleeves and just go with hot soap and water + hand sanitizer for the forearms.  It will save some money as well.


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Offlinethebug76
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Re: Contamination or normal? Pic [Re: shroomyaxn]
    #22147253 - 08/26/15 03:40 PM (8 years, 5 months ago)

UPDATE:  2 jars ended up having small amounts if metabolites, but started fading as they got closer to 100%. I used 6-1qt bowls to spawn to for now, so I could spawn those seperate. I used the double bucket tek for pasteurizing the hpoo. It worked like a charm. After I brought the sub to 160° I put the lid on, wrapped with a blanket and went to bed. Got up 8hrs later and it was still at 130°, so I feel pretty sure it held temps long enough. I should have taken more pics, I'm just not a picture person.


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