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Offlinemegatacular
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help me please? i just want one successful flush.
    #22110666 - 08/19/15 10:45 AM (8 years, 5 months ago)

Hello everyone. Inew to shroomery but not so much to cultivation I've been hard at it for a few years on and off and still can't seem to get one single monotub to fruit without those damn pesky moldy and.mildewy stupid contams. I've.mastered if I may say so my self prepping and fully colonizing my rye jars. (Quart). I can confidently go from spore syringe to    solid white brick o myc in two weeks. But here's where I fail horribly.

I've tried lots of sub recipes and usually.they all work up until about 60 to 70% colonized in tub then BWAAAAM!!! Here comes the mold every time. I've donenall I can to bemas clean and germ free as possible just short of building a megatacularly humungus air tight greenhouse looking glove box to put everythin . tub pasteurized sub. Spawn jars. I mean everything u can think of in it and atomic bomb that bitch with cans of Lysol and bottles of 91% alcohol misted through a pump up sprayer. I mean it shouldn't be this difficult right?

Please
Please
Someone give a little advise here I don't like begging but.man I do need to find out where I'm going wrong and fast. I'm getting so hungry it hurts. Lol
Thanks


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Offlinespacechildo
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110679 - 08/19/15 10:51 AM (8 years, 5 months ago)

just start by doing RR's rye prep http://www.mushroomvideos.com/Grain-Preparation

if you use a spore syringe just use 1 or 2 drops pr jar.
the best way to start would be to put spores on Pasty plates

spawn the grains to simple coir+verm which is hard/impossible to fuck up.

and post pics as you go along!


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: spacechildo]
    #22110789 - 08/19/15 11:11 AM (8 years, 5 months ago)

Yeah rrs tek. I do my grain exactly the way he.shows it in the vid. I have no problem with growing myc in jars. Just contams in substrate. My most used sub and casing recipe as follows

Sub
1 brick coir
2 quarts verm
4 to 6 quarts spwan
Or
1 brick coir
1 quart verm
1 quart spent coffee
1/2 quart gypsum
And 4 to 6 quarts spawn

And for casing I use this


5-6 qts peat (it shrinks when it gets wet)
1qt coarse vermiculite (fine, medium ok too)
3/4 cup limestone (finely ground best bet)
3/4 cup perlite

If i was adding hydrated lime to this, i would add about 1 heaping teaspoon (or about 1 level teaspoon per tub).


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110870 - 08/19/15 11:17 AM (8 years, 5 months ago)

Your contaminations are coming from your jars - not your substrate.

If you want clean spawn you need to step away from needles & inoculate w agar.

Also stop casing! No need in a bulk tub and it could also be part of your problem.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110876 - 08/19/15 11:18 AM (8 years, 5 months ago)

there's plenty of white molds. most green stuff you see on tubs are just the molds' spores.

how do you prep your coir+verm? do you cook it?

Its gotta be the grains if you do. post a pic of some if you can/have.

put a drop from your syringe on agar and see what grows. couldjust be a dirty syringe or 2 :shrug:


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110882 - 08/19/15 11:18 AM (8 years, 5 months ago)

www.shroomery.org/10858/How-to-do-Coir

I prep all my sub's just like this too. Casing is a little different I do it in jars at 5 psi in PC for an hr and a half. Casing never usually get contams even left a jar on shelf for two weeks and it didnt go bad.


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110911 - 08/19/15 11:23 AM (8 years, 5 months ago)

Quote:

megatacular said:
www.shroomery.org/10858/How-to-do-Coir

I prep all my sub's just like this too. Casing is a little different I do it in jars at 5 psi in PC for an hr and a half. Casing never usually get contams even left a jar on shelf for two weeks and it didnt go bad.





Or usually around 150 in and open test jar I have placed inside with remote thermometer I have rigged and sealed through my PC it welded to it to no worries there.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110925 - 08/19/15 11:26 AM (8 years, 5 months ago)

Mold before first flush is always the result of bad spawn.


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22110940 - 08/19/15 11:29 AM (8 years, 5 months ago)

Quote:

PirateSwazey said:
Your contaminations are coming from your jars - not your substrate.

If you want clean spawn you need to step away from needles & inoculate w agar.

Also stop casing! No need in a bulk tub and it could also be part of your problem.





Aaaaahahahaaa there's that pic again. Freaky man. Love it. And u really think it coulb be my syringes? I always use different ones each time new and flame sterilized. I have 1 syringe for each jar flame sterile so I don't cross contam. And no casing? I don't.like to open to mist or crack the seal on my tubs till they fruit. Unthink without casing it could go dry?


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22110970 - 08/19/15 11:31 AM (8 years, 5 months ago)

Quote:

PirateSwazey said:
Mold before first flush is always the result of bad spawn.




Huh. So if it doesn't.show up in my jars that doesnt.mean it isn't there?


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110982 - 08/19/15 11:32 AM (8 years, 5 months ago)

If you are using pasteurized coir and getting
contams before the first flush, it is definitely
your spawn that is bad.

You need to get into agar so you can isolate clean growth.

Most contaminant molds are white until the get a little
fresh air, then they sporulate which makes them appear
blue or green.

Inside a jar, it can be difficcult to differentiate
between clean growth and contam growth.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110984 - 08/19/15 11:32 AM (8 years, 5 months ago)

1 syringe pr jar is NOT 1 drop per jar.. dont sterilize peat. and its still your grains.


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110986 - 08/19/15 11:33 AM (8 years, 5 months ago)

Also if I use more spawn than nessisary do u think it would colonize faster than the.mold could?


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110994 - 08/19/15 11:35 AM (8 years, 5 months ago)

You don't mist tubs.. Just let them do their thing and stuff the holes with poly once they start pinning.

Set and forget! And nope I never ever ever case and always pull atleast 8 zips before I toss a sub out.

Yes your inoculation method is bad -- ask any serious grower they will tell you agar is the only way to go.

Quote:

megatacular said:
Quote:

PirateSwazey said:
Mold before first flush is always the result of bad spawn.




Huh. So if it doesn't.show up in my jars that doesnt.mean it isn't there?



Correct. Even after years of doing this and spawning close to 1000 tubs worth of spawn I still have trouble identifying some molds in spawn jars. But, working with a laminar flow hood and using agar to help insure clean cultures, I very rarely ever see a contamination until the 2nd flush is over when the mycelium has become weak.

Clean work makes the dream work :super:


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111000 - 08/19/15 11:35 AM (8 years, 5 months ago)

Quote:

megatacular said:h
Also if I use more spawn than nessisary do u think it would colonize faster than the.mold could?



No. If there is mold in your spawn, it will eventually
rear its ugly head


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Re: help me please? i just want one successful flush. [Re: natedawgnow]
    #22111031 - 08/19/15 11:38 AM (8 years, 5 months ago)

Quote:

natedawgnow said:
If you are using pasteurized coir and getting
contams before the first flush, it is definitely
your spawn that is bad.

You need to get into agar so you can isolate clean growth.

Most contaminant molds are white until the get a little
fresh air, then they sporulate which makes them appear
blue or green.

Inside a jar, it can be difficcult to differentiate
between clean growth and contam growth.





Wow man. I didn't.know that. That's very helpful. I will definaltly get into agar if that is possible to isolate just pure shroomage. How do I do that


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111064 - 08/19/15 11:43 AM (8 years, 5 months ago)

This is a great resource:

Stros cleaning up agar


--------------------


Edited by natedawgnow (08/19/15 11:45 AM)


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111065 - 08/19/15 11:43 AM (8 years, 5 months ago)

Same to u swazey how much am I looking at here to do agar cultures there called right? What all do I need I wanna make a list then watch videos on it. Any links? I'll so anything to get my mushies man. I've tryed so hard so long.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111072 - 08/19/15 11:45 AM (8 years, 5 months ago)

www.mushroomvideos.com

There's an agar and petri section. Just buy the DVD, it's worth it.

I like premixed agar, plastic Petris & a whisky bottle to sterilize in.


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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22111086 - 08/19/15 11:49 AM (8 years, 5 months ago)

Not if you're running manure. In that case both under cooking and over cooking can fuck things up (I do 140-160, closer to 140 for 60 minutes. Definitely noticed a difference by bringing the times down from 90 minutes), as well as having the substrate too wet(I've never seen a thread saying sub was made too dry, because you can just mist it, but I have seen tons of subs made too wet). Although if you do the bulk prep right, and it still contaminates, it's always the spawn that's the problem.

Bad spawn isn't always the result of bad inoculant. I upped my pc times to 2+ hours, and have noticed the bacterial endospores really gone down. What do you use for filters?  Using a syringe instead of clean af agar is a lot more risky, and when it contaminates you have no idea what to blame.

What's always to blame is your mycelium shit the bed. Not your environment.
Quote:

Mad Season said:
Um weakened mycelium comes from a huge array of things. Including spawn. Always ALWAYS work on improving your prep procedures.

Quote:

Mad Season said:
There's lots of reasons. All of them stemming from the fact the mycelium shit the bed. And if it does sooner than it should, it's a problem that should be fixed. bacteria(more bacteria) through improperly sterilized grains(I've upped my pc times and changed grains too many times to count, but I'm loving oats, I do most qt jars 2 hours @15-20psi), improper pasteurization, improper hydration, hidden contams from improper procedures and there's many more stuff I bet you could improve on.

P.S. I didn't say toss them all. Just the ones with trich, and to always work on improving the conditions for the myc. The healthier the myc, the better things will be :thumbup:



Quote:

Mad Season said:
Quote:

hiperhiper said:
so what's the right procedure with grains?
I've checked the teks and nobody said anything about this. I didn't know I was not supposed to use syringe on grains. What is cleaning up syringe on agar? what does it mean?




http://www.shroomery.org/forums/showflat.php/Number/18431006
http://www.shroomery.org/forums/showflat.php/Number/18430998
http://www.shroomery.org/forums/showflat.php/Number/19208976 http://www.shroomery.org/forums/showflat.php/Number/21889950/page/1




--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Edited by Mad Season (08/19/15 11:57 AM)


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Re: help me please? i just want one successful flush. [Re: Mad Season]
    #22111167 - 08/19/15 11:58 AM (8 years, 5 months ago)

People still use manure?  :hehehe:


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InvisibleMad Season
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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22111182 - 08/19/15 12:00 PM (8 years, 5 months ago)

When you've got this literally less than 100 steps outside your front door...

its all dry too. Just go grab and load it into boxes. Advantages of having a girlfriend who's a horse lover and breeder :P


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Re: help me please? i just want one successful flush. [Re: Mad Season]
    #22111217 - 08/19/15 12:16 PM (8 years, 5 months ago)

I wanna thank everyone.here this has really helped me understand theres more hope. I was so worried there was nothing else I could do. This is why I joined shroomery. You guys gave.me.hope again. YAAAY. I can't wait.to trynthis agar thingy and cook the fuck out.of.my grains. No casing this time and just the.basic coir and verm sub. Or should I add coffee and gypsum for nutrients? I'm going for a flush so thick I can't.see past the.caps a full on blanket of mushie goodness. Will just coir verm and grain get me that?


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111347 - 08/19/15 12:37 PM (8 years, 5 months ago)

So basicly when doing agar. U use syringe spores to knock up agar then cut out the biggest beefyest looking strain at the outer most.edge where its all fanned out then put in other agar and grow again till u get only one uniform strain or growth that perfectly round then  cut little peaces  of the outter most edges and drop into jars o grain? All while in front of flow hood or still air box? Then what throw away remaining agar? And start from spores again? Eerrr what am I missing?


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111378 - 08/19/15 12:38 PM (8 years, 5 months ago)

Oh do I need an incubator for agar? Cause that too expensive for me


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111389 - 08/19/15 12:39 PM (8 years, 5 months ago)

Don't incubate anything EVER.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Re: help me please? i just want one successful flush. [Re: Mad Season]
    #22111529 - 08/19/15 12:49 PM (8 years, 5 months ago)

I saw how u do it its really that hard looks like I mean u did it in a tubaware thing straight into a jar no flow hood or still air.box right? Open air no gloves.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111558 - 08/19/15 12:50 PM (8 years, 5 months ago)

I mean not that hard


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111591 - 08/19/15 12:53 PM (8 years, 5 months ago)

Am I correct. On what all to do? Just.keep cutting. Pieces till a dish is only one strain then drop a piece in a jar and grow it?


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111646 - 08/19/15 12:56 PM (8 years, 5 months ago)

OUP nope.thentowel is the imaginary still air.box. got.it. OK


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111752 - 08/19/15 01:03 PM (8 years, 5 months ago)

Yes coir & verm will give you good flushes. You can add gypsum if you like but I would not add coffee... ever.

Don't worry about isolating a strain just make sure you have clean mycelium by making a few rapid transfers (as soon as there is enough growth to grab a piece the size of a grain of rice). If you want to do strain isolation (not necessary for cubes IMO) - work off of a clone culture, not multispore. That will ensure you have more fruiting genes in your gene pool.

My best cultures were all obtained via this method then simply cloning fresh mushroom tissue. Once I have a clone I put a wedge into a test tube slant and that will go in a mini fridge for cold storage, where the strain will be accessible for about 2 years.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111851 - 08/19/15 01:09 PM (8 years, 5 months ago)

Deffinatly my spawn and my paturizing.too I'm sure.looking back now on my grows. My jars did fully colonize but not clean. Some I'm pretty sure did and they ended up getting black and green.mold in the.tub while the unclean ones got white orange or yellow even brown molds I'm guessing.my clean ones had.bad sub and unclean had good.sub just terrible spawn. Or both had.both or all had the same and Same is all and yeah I think I get it now guys. I really really appreciate everything I'll use yalls advise and post pics and a grow log soon hope y'all check it.out and tell me what y'all think


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111905 - 08/19/15 01:16 PM (8 years, 5 months ago)

Quote:

PirateSwazey said:
Yes coir & verm will give you good flushes. You can add gypsum if you like but I would not add coffee... ever.

Don't worry about isolating a strain just make sure you have clean mycelium by making a few rapid transfers (as soon as there is enough growth to grab a piece the size of a grain of rice). If you want to do strain isolation (not necessary for cubes IMO) - work off of a clone culture, not multispore. That will ensure you have more fruiting genes in your gene pool.

My best cultures were all obtained via this method then simply cloning fresh mushroom tissue. Once I have a clone I put a wedge into a test tube slant and that will go in a mini fridge for cold storage, where the strain will be accessible for about 2 years.




Whoa man. 2 years?. Whats a clone? And a wedge? And culture slant? Sorry this is all new to me.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22112086 - 08/19/15 01:56 PM (8 years, 5 months ago)

Download those videos I linked you to


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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22112135 - 08/19/15 02:07 PM (8 years, 5 months ago)

Thats a big 10-4 there scary face man. Is the download the same as the DVDs? I mean does it come with all the chapters the dvds do


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22112142 - 08/19/15 02:08 PM (8 years, 5 months ago)

And I'm gonna give u a good rating


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22112155 - 08/19/15 02:10 PM (8 years, 5 months ago)

Crap have to have 50 post to rate someone.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22112159 - 08/19/15 02:10 PM (8 years, 5 months ago)

48


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22112163 - 08/19/15 02:11 PM (8 years, 5 months ago)

49


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22112164 - 08/19/15 02:11 PM (8 years, 5 months ago)

50 boooooommm


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22112259 - 08/19/15 02:34 PM (8 years, 5 months ago)

Yes the download is just cheaper.

Thanks mate :super:


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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22112289 - 08/19/15 02:39 PM (8 years, 5 months ago)

No thank u. I got a warning.just now for bumping. Didn't.know what that.meant but I do now that do show. Can't wait to get growing.I'm stoked on what y'all showed.me. I've.franticly ordering stuff from sponsors lol.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22119715 - 08/21/15 12:59 AM (8 years, 5 months ago)

So for a little update here. I ordered everything for making mea agar plate. Got the petris parafilm building a brand new better still air box. Wanted a straight glove box but no way to use a flame inside one unless u wanna blow ur face off. Got new coir bricks coarse verm new rye berries jar lids PC spores. Only thing is to build two new.mono tubs and I'll be set. Till I start doing slants then I'll buy more stuff. Haven't got that far yet but I will. I will start a log soon hope everyone checks it.out and tells me what they think.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22121028 - 08/21/15 07:58 AM (8 years, 5 months ago)

Sounds great... you won't need to worry about slants for awhile anyway.

It's much better to not attach gloves to your box.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22121539 - 08/21/15 09:43 AM (8 years, 5 months ago)

Quote:

megatacular said:
Wanted a straight glove box but no way to use a flame inside one unless u wanna blow ur face off.



NEVER have a flame inside any kind of box.when using a SAB you want the air still,flames create air currents and are dangerous.
you should also stop using the cans of Lysol and bottles of 91% alcohol to spray inside your box,use soapy water only.WIPE everything with 70% iso,not 91%.good luck.


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Re: help me please? i just want one successful flush. [Re: KNOTHEAR]
    #22122782 - 08/21/15 02:31 PM (8 years, 5 months ago)

Yeah man I know plus I just don't like glove boxes anyway. Also I did over exaggerate a bit about cans and bottles I don't use that much. So ur suggesting just wash everything like a dish then put into box and wipe with alcohol? What about the.air inside the box? If mold is in it won't it settle on my work? I mean a bleached soaked blanket I keep on bottom I hope takes care of most but....

Basicly what I'm trying to do is be as.absolutely clean. Sterile. And.sanitary as possible here. To destroy any change.of.contams I've.felt with so much lately.

And why not 91%? Isn't it better?


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22122803 - 08/21/15 02:35 PM (8 years, 5 months ago)

Impossible to have a sterile environment in a still air box. The key is to be thorough and not let anything hover over open media. When  you take off lids and shit don't let anything go over your open stuff.

http://www.shroomery.org/forums/showflat.php/Number/21889950


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Re: help me please? i just want one successful flush. [Re: Mad Season]
    #22122960 - 08/21/15 03:04 PM (8 years, 5 months ago)

I would isolate on agar then make a large amount of liquid culture. I then put a drop of my liquid culture on agar to make sure still contam free then I use liquid culture to inoculate grains or brf cakes look at my pics in profile that was pf tech first try I found the 4 cup pp5 did best for me got around 2oz dry each vrs 1/2 to 1 from the small jars. I only do 2 flushes and toss. Rr and others have done the foot work hope this info helps. I have done 2 36qt monos and I just used still air box and crumbled colonised cakes to spawn to 10qtsi used 6 1/2 pint pf Tec cakes and after colonised I put a very thin layer of dry verm  for casing and let it go till I saw pins then polly fae holes then more munchies then my dryer could handle. Good luck


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Re: help me please? i just want one successful flush. [Re: Mad Season]
    #22123008 - 08/21/15 03:15 PM (8 years, 5 months ago)

Quote:

Mad Season said:
Impossible to have a sterile environment in a still air box. The key is to be thorough and not let anything hover over open media. When  you take off lids and shit don't let anything go over your open stuff.

http://www.shroomery.org/forums/showflat.php/Number/21889950




Well I will be extra careful in handling media and do as best as I can using.all the best teks and advise from u guys. I really appreciate everything.


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Re: help me please? i just want one successful flush. [Re: KNOTHEAR]
    #22123016 - 08/21/15 03:17 PM (8 years, 5 months ago)

70% penetrates and kills better than 91%.use the 70% to WIPE the inside of the SAB and everything you put in it except the needles you use.have everything you need INSIDE the SAB except your flame and needles.fill your mist bottle with soapy water and spray through arm holes.wait a few minutes for germs and spores to fall. Good to go :thumbup:


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Re: help me please? i just want one successful flush. [Re: KNOTHEAR]
    #22123031 - 08/21/15 03:21 PM (8 years, 5 months ago)

I'd say wait at least 20 minutes. Takes a particle at 5 microns 1 hour to settle to the ground 5 feet in the air.


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Re: help me please? i just want one successful flush. [Re: Mad Season]
    #22123097 - 08/21/15 03:37 PM (8 years, 5 months ago)

Quote:

Mad Season said:
I'd say wait at least 20 minutes. Takes a particle at 5 microns 1 hour to settle to the ground 5 feet in the air.




Noted I didn't know that type mad


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Re: help me please? i just want one successful flush. [Re: fear-and-loathing]
    #22125433 - 08/22/15 02:47 AM (8 years, 5 months ago)

Wow good info guys. I will add it too my notes


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22127246 - 08/22/15 02:19 PM (8 years, 5 months ago)

Another ?   

Could I use what's left over from my tyvek suits to put on fae holes of my tubs or is poly fil the best to use.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22127251 - 08/22/15 02:21 PM (8 years, 5 months ago)

Polys the best imo. Easy to tune


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Re: help me please? i just want one successful flush. [Re: Mad Season]
    #22127263 - 08/22/15 02:23 PM (8 years, 5 months ago)

Yeah I just hate trying to get it to stay in the holes just right. I'll just try my two tubs with poly and one with tyvek. See how it goes for me.

How tight isbtoo tight when stuffing holes with the poly?


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22127285 - 08/22/15 02:26 PM (8 years, 5 months ago)

dial your tubs in like a boss use this as a starting point.


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Re: help me please? i just want one successful flush. [Re: spacechildo]
    #22127330 - 08/22/15 02:37 PM (8 years, 5 months ago)

Cool. So I bacicly wanna force the poly into the holes on the bottom and just barely place it ever so gently into the top holes in a lightly poofed ball o plasty strands. Gotcha


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22127348 - 08/22/15 02:41 PM (8 years, 5 months ago)

I'm so excited I have everything now. I'm using two 20qt and one 25q tubs to start out with with sub levels at 3". They're only 19" high and I want enough room for the fruits to grow and.not.get crushed but they probably will. Lol well see if I can get them to grow first.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22127371 - 08/22/15 02:45 PM (8 years, 5 months ago)

pasty likes mini monos just do what he does! :yesnod:


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Re: help me please? i just want one successful flush. [Re: spacechildo]
    #22128254 - 08/22/15 07:01 PM (8 years, 5 months ago)

Sweet. To my notes it.is added my friend thanks.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22128486 - 08/22/15 08:11 PM (8 years, 5 months ago)

Quote:

megatacular said:
www.shroomery.org/10858/How-to-do-Coir

I prep all my sub's just like this too. Casing is a little different I do it in jars at 5 psi in PC for an hr and a half. Casing never usually get contams even left a jar on shelf for two weeks and it didnt go bad.



No one noticed this. If this isn't a typo and he was casing all his tubs with peat pcd at only 5 psi for an hour id say this was a megafail casing procedure from the get


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Re: help me please? i just want one successful flush. [Re: professorFATTYCAP]
    #22129302 - 08/23/15 12:36 AM (8 years, 5 months ago)

Quote:

professorFATTYCAP said:
Quote:

megatacular said:
www.shroomery.org/10858/How-to-do-Coir

I prep all my sub's just like this too. Casing is a little different I do it in jars at 5 psi in PC for an hr and a half. Casing never usually get contams even left a jar on shelf for two weeks and it didnt go bad.



No one noticed this. If this isn't a typo and he was casing all his tubs with peat pcd at only 5 psi for an hour id say this was a megafail casing procedure from the get




Yep it was.now I'm gonna try no casing at all. And that was before I found shroomery. I mean it worked sometimes. But not gonna do it till I know what I'm doing. I'm working really hard this go around so I hope to see some results. I mean I've changed the way I'm gonna do everything now. To the most updated tens and methods used by you guys.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22129972 - 08/23/15 08:44 AM (8 years, 5 months ago)

You really really don't need to case cubes unless it's PE.


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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22132890 - 08/23/15 09:03 PM (8 years, 5 months ago)

Updates my neegies.

Built my two mini monos. And got.my sab done. Please help me to post pics on thread. I don't.know how. I'm using my phone.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22132907 - 08/23/15 09:07 PM (8 years, 5 months ago)

android? if you have them uploaded put your finger on the code under the pic and hold it till it says"copy" then copy it and hold your finger on the reply box till it says pasty


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Re: help me please? i just want one successful flush. [Re: newera]
    #22132909 - 08/23/15 09:07 PM (8 years, 5 months ago)

*paste, sorry been on pasty's thread for awhile lol


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Re: help me please? i just want one successful flush. [Re: newera]
    #22132935 - 08/23/15 09:14 PM (8 years, 5 months ago)

Updates my neegies.

Built my two mini monos. And got.my sab done hope you peeps Luke. Comments please and thanks



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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22132956 - 08/23/15 09:20 PM (8 years, 5 months ago)

This is my other tub. The 25 at. The other two were 20s. Its my experimental tub.




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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22134981 - 08/24/15 10:53 AM (8 years, 5 months ago)

is that liner on the outside of the tote.if so ..u gotz 2 b putn dat shits on d inside brudda man. the concept isnt 2 keep out light its 2 form a tite fittng bond 2 the edge of the sub 2 prevent side pinning


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Re: help me please? i just want one successful flush. [Re: professorFATTYCAP]
    #22136273 - 08/24/15 04:22 PM (8 years, 5 months ago)

Yeah liner goes inside


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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22136581 - 08/24/15 05:26 PM (8 years, 5 months ago)

Oh yeah idk what I painted em for. Just got bored. I entended on using a garbage bag liners on the inside. Lol I knew that one.

I got my spores today!!!! Yay.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22136610 - 08/24/15 05:32 PM (8 years, 5 months ago)

The tubs were as close to a flush fit on the lids. Almost sealed. I was wondering. Does it matter about sealing the lid. I see some people tape and shrink wrap the lids. Is that necessary? Or is it ok to have a little gap under the lid


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22136762 - 08/24/15 06:06 PM (8 years, 5 months ago)

Most people just pop the lid into place and let it colonize. Unless your using a different lid that doesn't latch into place properly, you shouldn't worry about taping it up, remember, you want some GE for your mono. The small gaps in a properly fitted lid will supply all the GE needed. :thumbup:


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Re: help me please? i just want one successful flush. [Re: Psilosoulful]
    #22136812 - 08/24/15 06:16 PM (8 years, 5 months ago)

Oh cool. Yeah they fit well and snap into place. I dug around the shelf at Walmart and got the best two I could find.
Just waiting on my agar and Petri's to come in the mail this week sometime and I'll be good to go. I'll start a grow log and share all my steps taken


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22159382 - 08/29/15 06:43 AM (8 years, 5 months ago)

OK so I've done 12 agar plates so far and inoculated them with spore syringes. I did it on the 26th and today I can barely see little white dots where the spores were.
Here's my question

Is it better to knock up grains first then transfer a grain to the agar? Would this be easyer?

I kind of feel like some of my dishes arentoo dey and others have water puddles when I tilt them but have more growth that ones that look dry I'll try and getnsome good pics


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22159407 - 08/29/15 06:58 AM (8 years, 5 months ago)

How much solution did you squirt into the dish? One or two drops is all you need. Then roll the solution around to touch every part of the plate.

If your pasty plates have condensation in them from the PC cycle, the excess moisture will evaporate in a couple of days, as long as you have a GE hole.


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Re: help me please? i just want one successful flush. [Re: Psilosoulful]
    #22160474 - 08/29/15 12:02 PM (8 years, 5 months ago)

Well for one I'm using the pre sterile plastic Petri's.

2 I did one drop on the first set of six
Then two drops on the second set of six

Then taped using clear scotch tape.... The kind that folds and.makes creases and doesn't stretch. Should I not have.done that? I lost my parafilm I ordered. Would micropore tape be better for GE?

Give me a little while to get home.and I'll post some pics


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22160726 - 08/29/15 01:12 PM (8 years, 5 months ago)

It should work. Lots of people just wrap with saran wrap.


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Re: help me please? i just want one successful flush. [Re: Mad Season]
    #22160787 - 08/29/15 01:28 PM (8 years, 5 months ago)

Yeah I hope. I used as sterile of teknique in my sab as I possibly could. Moving.very slow and not working.over any exposed media. I'll be home shortly then I'll add the.pix. then we can go from there.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22161125 - 08/29/15 02:57 PM (8 years, 5 months ago)

OK so I'm gonna try and sort these out for you guys. The six on the top I did on the 26th the bottom six I did on the 28th


This is the one from the middle row on the top. Is it growing mycelium? Looks milky but fluffy white in middle
This one is from the second row from top and is the middle dish.
4 more are similar to the above pics then I have a few that to me look dry even ones I put 2 drops in and look like this
And also this

They have very little white spots and some do not have.anything I can in fact still see the.purple spores
Every opinion is greatly appreciated


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OfflineKNOTHEAR
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22164420 - 08/30/15 11:42 AM (8 years, 4 months ago)

stop looking at them so much,your freaking them the f**k out:eek:.put them somewhere and don't look at the plates again until Wednesday,Sept 3rd. :whistling:


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OfflinePsilosoulful

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Re: help me please? i just want one successful flush. [Re: KNOTHEAR]
    #22164435 - 08/30/15 11:47 AM (8 years, 4 months ago)

Idk those plates look bacterial. Don't see any mycelium growing there. But put them away for now and see if any mycelium grows. You can transfer away from the bacterial blotches to a new plate.


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Re: help me please? i just want one successful flush. [Re: Psilosoulful]
    #22164948 - 08/30/15 02:01 PM (8 years, 4 months ago)

Ha yeah. I think I got a bogus b+ syringe. But yeah I do worry about. Em way too damn much. I'll send an update on the third or 4th depending what my plates feel like doing. Lol. I'll let them decide. Give my little baby the love and care it needs the leave it the fuck alone till its ready to say hi to daddy right? Lol


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22171339 - 08/31/15 09:17 PM (8 years, 4 months ago)

Update!!!!
Theyre alive people they're ALIVE!!!!!!
Today I woke up and fuckin BWAAAM!!!!


Bitches beez groweeeeenn


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22171412 - 08/31/15 09:32 PM (8 years, 4 months ago)

Looks moldy in there. :feelsbadman:


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Re: help me please? i just want one successful flush. [Re: Psilosoulful]
    #22171548 - 08/31/15 09:59 PM (8 years, 4 months ago)

God damnit don't u even. I'm growing it anyway fuck it. Lol


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22171576 - 08/31/15 10:06 PM (8 years, 4 months ago)

Quote:

megatacular said:
God damnit don't u even. I'm growing it anyway fuck it. Lol




are those spore plates? could just be multiple germination points of cube myc.


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Re: help me please? i just want one successful flush. [Re: Munchauzen]
    #22171587 - 08/31/15 10:07 PM (8 years, 4 months ago)

Yeah ms plates. And I swished them after I blew a load in em


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22171610 - 08/31/15 10:10 PM (8 years, 4 months ago)

yeah you're fine then. you will want to transfer asap, though. its best not to let those islands merge together, though. the less genetics the better. starting from spores, you are already working with a large set of genetics on those plate.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22171622 - 08/31/15 10:13 PM (8 years, 4 months ago)

Whenever I do ms on agar I just put the spores in the center. That way u know the only place that should grow something is in the center anything away from center growing I know it's contams. Plus when I did spores it took a long as time for them to germinate and grow like 3-4 weeks I actually gave up on it and forgot about it so I'm not exactly sure how long it took but I gave up after 2 weeks of nothing.


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Re: help me please? i just want one successful flush. [Re: more4u2c]
    #22171652 - 08/31/15 10:21 PM (8 years, 4 months ago)

when I inoculate agar with a spore print, I use a presterilized medical swab and streak on a plate in a pattern like this




since the print has many more cube spores on it with only a relavtively small amount of contaminates, this pattern will distribute the spores across the plate, leaving the majority of the contaminates at the #1 inoculation area.

note the #1 pattern is zigzagging. you are trying to get as many spores on the plate as possible.

pattern #2 is to pull large amounts of mostly cube spores away from the inital contact area. note the zigzagging pattern has stopped and we are pulling straight lines now.

pattern #3 should be nothing but cube spores at this point and any growth from this area is generally going to be favored over other growth sites.


Edited by Munchauzen (08/31/15 10:23 PM)


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Re: help me please? i just want one successful flush. [Re: Munchauzen]
    #22171834 - 08/31/15 10:54 PM (8 years, 4 months ago)

Thanks guys. I figured since one so far does have bacteria or contams and nothing else looks likt it has any growth really. Maybe they do. Idk. This white suff where spores used to be has to be myc. So does everyone agree I should transfer now? Taking each island to a new plate?


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22172409 - 09/01/15 03:07 AM (8 years, 4 months ago)

I like to wait until I see some rhizo growth and then take one Lil phat rhizome strand.


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Re: help me please? i just want one successful flush. [Re: more4u2c]
    #22172577 - 09/01/15 05:46 AM (8 years, 4 months ago)

I do see some ryzos since there's quite a bit. I'll transfer some now and later too. See what happens


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22181567 - 09/03/15 02:02 AM (8 years, 4 months ago)

WHOOOH!!!! Update assholes!!
Day 9 of my new and improved grow. And hot damn I'm excited. Made my first.transfers on the first and them babies are taking.off. also knocked up some rye jars for the hell of it and ordered me some new shit. Added two more 20q monos to the grow as well check out my babies. There getting so big.


This is the the original ms culture. Still no contams.:smile:



And the transferes. I used distilled ro water and it made them way more clear for some reason. Awesome




Let me have it guys. Whatcha think?


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22182934 - 09/03/15 12:49 PM (8 years, 4 months ago)

forget about using distilled water. it has no purpose in our hobby and does nothing but put more plastic in our landfills.


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Re: help me please? i just want one successful flush. [Re: Munchauzen]
    #22183455 - 09/03/15 02:58 PM (8 years, 4 months ago)

Quote:

Munchauzen said:
forget about using distilled water. it has no purpose in our hobby and does nothing but put more plastic in our landfills.




I'm with u there. But I make my own. I have an ro di system under my sink I use to top off and change water in my saltwater reef tank. Then I have a copper worm can connect to my PC that cools in a 5 gal bucket and condenses the steam and into the.same gal jug again.
Plus The water in my city is very poor quality and high in chlorine


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22185877 - 09/03/15 11:28 PM (8 years, 4 months ago)

:adventuretime:


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22185894 - 09/03/15 11:38 PM (8 years, 4 months ago)

Quote:

megatacular said:
Quote:

Munchauzen said:
forget about using distilled water. it has no purpose in our hobby and does nothing but put more plastic in our landfills.




I'm with u there. But I make my own. I have an ro di system under my sink I use to top off and change water in my saltwater reef tank. Then I have a copper worm can connect to my PC that cools in a 5 gal bucket and condenses the steam and into the.same gal jug again.
Plus The water in my city is very poor quality and high in chlorine



The chlorine content means little....my tap also smells like the local pool.....it works just fine.


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Re: help me please? i just want one successful flush. [Re: PussyFart]
    #22186023 - 09/04/15 12:20 AM (8 years, 4 months ago)

Ah. Well I mean I have it free so why not right? Good to know though. Thanks. Just a little expirement idid.

Oh and.pirate! Wtf man? Really?


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22186810 - 09/04/15 08:17 AM (8 years, 4 months ago)

lol just thought it was funny how munch assumed u were some kinda nerd packing in h2o and instead you're all nah.. actually I'm a reef keeper with a RO kit under da sink :smirk:


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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22188222 - 09/04/15 01:53 PM (8 years, 4 months ago)

Ha yeah. Got sick of buying premixed saltwater from the pet store that killed my coral so I saved shit tons and bought an ro system and all my chems nutes mins and mets to make my own. Cheaper in the long run and i don't have to haul around 5gal jugs anymore. My di water system I separate though I just use it for cleaning stuff.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22217738 - 09/10/15 05:06 PM (8 years, 4 months ago)

update.

It a been 10 days so far since I transferred these from the original ms dishes and they're doing great. No contams. All seem to need more transferres but this one I'm not so sure about could it really be a mono already?



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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22217905 - 09/10/15 05:45 PM (8 years, 4 months ago)

Quote:

megatacular said:
update.

It a been 10 days so far since I transferred these from the original ms dishes and they're doing great. No contams. All seem to need more transferres but this one I'm not so sure about could it really be a mono already?








somebody nominate that plate for pic of the month! freakin' beautiful!


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Re: help me please? i just want one successful flush. [Re: Munchauzen]
    #22218043 - 09/10/15 06:21 PM (8 years, 4 months ago)

Ha you really think so? Thanks man. So do u think its a.mono? Or do you see sectors? I cantnreally tell


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22218047 - 09/10/15 06:23 PM (8 years, 4 months ago)

Looks like it's a monoculture


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OfflinePsilosoulful

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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22218164 - 09/10/15 06:44 PM (8 years, 4 months ago)

Quote:

megatacular said:




Yea, that's a monoculture alright. Great job!! :awecid:


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Re: help me please? i just want one successful flush. [Re: Psilosoulful]
    #22218201 - 09/10/15 06:52 PM (8 years, 4 months ago)

No shit! Badass. First try baby!!! Whooh. That's cool . so I'm gonna make 12 more transferres  of this one. And start knocking shit up. What do I do with all the others? I don't have enough dishes.


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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22222070 - 09/11/15 01:42 PM (8 years, 4 months ago)

OK. I'll just throw the other dishes away then. Damn are people afraid of wittle bitty curse words or something?


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