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Offlinemegatacular
professor procrastinate
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Registered: 08/18/15
Posts: 183
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22161125 - 08/29/15 02:57 PM (8 years, 5 months ago)

OK so I'm gonna try and sort these out for you guys. The six on the top I did on the 26th the bottom six I did on the 28th


This is the one from the middle row on the top. Is it growing mycelium? Looks milky but fluffy white in middle
This one is from the second row from top and is the middle dish.
4 more are similar to the above pics then I have a few that to me look dry even ones I put 2 drops in and look like this
And also this

They have very little white spots and some do not have.anything I can in fact still see the.purple spores
Every opinion is greatly appreciated


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OfflineKNOTHEAR
Stranger

Registered: 08/05/14
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Last seen: 6 years, 9 months
Re: help me please? i just want one successful flush. [Re: megatacular]
    #22164420 - 08/30/15 11:42 AM (8 years, 4 months ago)

stop looking at them so much,your freaking them the f**k out:eek:.put them somewhere and don't look at the plates again until Wednesday,Sept 3rd. :whistling:


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
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Re: help me please? i just want one successful flush. [Re: KNOTHEAR]
    #22164435 - 08/30/15 11:47 AM (8 years, 4 months ago)

Idk those plates look bacterial. Don't see any mycelium growing there. But put them away for now and see if any mycelium grows. You can transfer away from the bacterial blotches to a new plate.


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: Psilosoulful]
    #22164948 - 08/30/15 02:01 PM (8 years, 4 months ago)

Ha yeah. I think I got a bogus b+ syringe. But yeah I do worry about. Em way too damn much. I'll send an update on the third or 4th depending what my plates feel like doing. Lol. I'll let them decide. Give my little baby the love and care it needs the leave it the fuck alone till its ready to say hi to daddy right? Lol


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22171339 - 08/31/15 09:17 PM (8 years, 4 months ago)

Update!!!!
Theyre alive people they're ALIVE!!!!!!
Today I woke up and fuckin BWAAAM!!!!


Bitches beez groweeeeenn


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OfflinePsilosoulful

Registered: 09/05/14
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22171412 - 08/31/15 09:32 PM (8 years, 4 months ago)

Looks moldy in there. :feelsbadman:


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: Psilosoulful]
    #22171548 - 08/31/15 09:59 PM (8 years, 4 months ago)

God damnit don't u even. I'm growing it anyway fuck it. Lol


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InvisibleMunchauzen
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22171576 - 08/31/15 10:06 PM (8 years, 4 months ago)

Quote:

megatacular said:
God damnit don't u even. I'm growing it anyway fuck it. Lol




are those spore plates? could just be multiple germination points of cube myc.


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: Munchauzen]
    #22171587 - 08/31/15 10:07 PM (8 years, 4 months ago)

Yeah ms plates. And I swished them after I blew a load in em


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InvisibleMunchauzen
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22171610 - 08/31/15 10:10 PM (8 years, 4 months ago)

yeah you're fine then. you will want to transfer asap, though. its best not to let those islands merge together, though. the less genetics the better. starting from spores, you are already working with a large set of genetics on those plate.


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Offlinemore4u2c
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22171622 - 08/31/15 10:13 PM (8 years, 4 months ago)

Whenever I do ms on agar I just put the spores in the center. That way u know the only place that should grow something is in the center anything away from center growing I know it's contams. Plus when I did spores it took a long as time for them to germinate and grow like 3-4 weeks I actually gave up on it and forgot about it so I'm not exactly sure how long it took but I gave up after 2 weeks of nothing.


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InvisibleMunchauzen
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Re: help me please? i just want one successful flush. [Re: more4u2c]
    #22171652 - 08/31/15 10:21 PM (8 years, 4 months ago)

when I inoculate agar with a spore print, I use a presterilized medical swab and streak on a plate in a pattern like this




since the print has many more cube spores on it with only a relavtively small amount of contaminates, this pattern will distribute the spores across the plate, leaving the majority of the contaminates at the #1 inoculation area.

note the #1 pattern is zigzagging. you are trying to get as many spores on the plate as possible.

pattern #2 is to pull large amounts of mostly cube spores away from the inital contact area. note the zigzagging pattern has stopped and we are pulling straight lines now.

pattern #3 should be nothing but cube spores at this point and any growth from this area is generally going to be favored over other growth sites.


Edited by Munchauzen (08/31/15 10:23 PM)


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: Munchauzen]
    #22171834 - 08/31/15 10:54 PM (8 years, 4 months ago)

Thanks guys. I figured since one so far does have bacteria or contams and nothing else looks likt it has any growth really. Maybe they do. Idk. This white suff where spores used to be has to be myc. So does everyone agree I should transfer now? Taking each island to a new plate?


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Offlinemore4u2c
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22172409 - 09/01/15 03:07 AM (8 years, 4 months ago)

I like to wait until I see some rhizo growth and then take one Lil phat rhizome strand.


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: more4u2c]
    #22172577 - 09/01/15 05:46 AM (8 years, 4 months ago)

I do see some ryzos since there's quite a bit. I'll transfer some now and later too. See what happens


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22181567 - 09/03/15 02:02 AM (8 years, 4 months ago)

WHOOOH!!!! Update assholes!!
Day 9 of my new and improved grow. And hot damn I'm excited. Made my first.transfers on the first and them babies are taking.off. also knocked up some rye jars for the hell of it and ordered me some new shit. Added two more 20q monos to the grow as well check out my babies. There getting so big.


This is the the original ms culture. Still no contams.:smile:



And the transferes. I used distilled ro water and it made them way more clear for some reason. Awesome




Let me have it guys. Whatcha think?


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InvisibleMunchauzen
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22182934 - 09/03/15 12:49 PM (8 years, 4 months ago)

forget about using distilled water. it has no purpose in our hobby and does nothing but put more plastic in our landfills.


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Offlinemegatacular
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Registered: 08/18/15
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Re: help me please? i just want one successful flush. [Re: Munchauzen]
    #22183455 - 09/03/15 02:58 PM (8 years, 4 months ago)

Quote:

Munchauzen said:
forget about using distilled water. it has no purpose in our hobby and does nothing but put more plastic in our landfills.




I'm with u there. But I make my own. I have an ro di system under my sink I use to top off and change water in my saltwater reef tank. Then I have a copper worm can connect to my PC that cools in a 5 gal bucket and condenses the steam and into the.same gal jug again.
Plus The water in my city is very poor quality and high in chlorine


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InvisiblePirateSwazey
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22185877 - 09/03/15 11:28 PM (8 years, 4 months ago)

:adventuretime:


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22185894 - 09/03/15 11:38 PM (8 years, 4 months ago)

Quote:

megatacular said:
Quote:

Munchauzen said:
forget about using distilled water. it has no purpose in our hobby and does nothing but put more plastic in our landfills.




I'm with u there. But I make my own. I have an ro di system under my sink I use to top off and change water in my saltwater reef tank. Then I have a copper worm can connect to my PC that cools in a 5 gal bucket and condenses the steam and into the.same gal jug again.
Plus The water in my city is very poor quality and high in chlorine



The chlorine content means little....my tap also smells like the local pool.....it works just fine.


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