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Offlinemegatacular
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help me please? i just want one successful flush.
    #22110666 - 08/19/15 10:45 AM (8 years, 5 months ago)

Hello everyone. Inew to shroomery but not so much to cultivation I've been hard at it for a few years on and off and still can't seem to get one single monotub to fruit without those damn pesky moldy and.mildewy stupid contams. I've.mastered if I may say so my self prepping and fully colonizing my rye jars. (Quart). I can confidently go from spore syringe to    solid white brick o myc in two weeks. But here's where I fail horribly.

I've tried lots of sub recipes and usually.they all work up until about 60 to 70% colonized in tub then BWAAAAM!!! Here comes the mold every time. I've donenall I can to bemas clean and germ free as possible just short of building a megatacularly humungus air tight greenhouse looking glove box to put everythin . tub pasteurized sub. Spawn jars. I mean everything u can think of in it and atomic bomb that bitch with cans of Lysol and bottles of 91% alcohol misted through a pump up sprayer. I mean it shouldn't be this difficult right?

Please
Please
Someone give a little advise here I don't like begging but.man I do need to find out where I'm going wrong and fast. I'm getting so hungry it hurts. Lol
Thanks


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Offlinespacechildo
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110679 - 08/19/15 10:51 AM (8 years, 5 months ago)

just start by doing RR's rye prep http://www.mushroomvideos.com/Grain-Preparation

if you use a spore syringe just use 1 or 2 drops pr jar.
the best way to start would be to put spores on Pasty plates

spawn the grains to simple coir+verm which is hard/impossible to fuck up.

and post pics as you go along!


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: spacechildo]
    #22110789 - 08/19/15 11:11 AM (8 years, 5 months ago)

Yeah rrs tek. I do my grain exactly the way he.shows it in the vid. I have no problem with growing myc in jars. Just contams in substrate. My most used sub and casing recipe as follows

Sub
1 brick coir
2 quarts verm
4 to 6 quarts spwan
Or
1 brick coir
1 quart verm
1 quart spent coffee
1/2 quart gypsum
And 4 to 6 quarts spawn

And for casing I use this


5-6 qts peat (it shrinks when it gets wet)
1qt coarse vermiculite (fine, medium ok too)
3/4 cup limestone (finely ground best bet)
3/4 cup perlite

If i was adding hydrated lime to this, i would add about 1 heaping teaspoon (or about 1 level teaspoon per tub).


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InvisiblePirateSwazey
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110870 - 08/19/15 11:17 AM (8 years, 5 months ago)

Your contaminations are coming from your jars - not your substrate.

If you want clean spawn you need to step away from needles & inoculate w agar.

Also stop casing! No need in a bulk tub and it could also be part of your problem.


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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Offlinespacechildo
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110876 - 08/19/15 11:18 AM (8 years, 5 months ago)

there's plenty of white molds. most green stuff you see on tubs are just the molds' spores.

how do you prep your coir+verm? do you cook it?

Its gotta be the grains if you do. post a pic of some if you can/have.

put a drop from your syringe on agar and see what grows. couldjust be a dirty syringe or 2 :shrug:


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110882 - 08/19/15 11:18 AM (8 years, 5 months ago)

www.shroomery.org/10858/How-to-do-Coir

I prep all my sub's just like this too. Casing is a little different I do it in jars at 5 psi in PC for an hr and a half. Casing never usually get contams even left a jar on shelf for two weeks and it didnt go bad.


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110911 - 08/19/15 11:23 AM (8 years, 5 months ago)

Quote:

megatacular said:
www.shroomery.org/10858/How-to-do-Coir

I prep all my sub's just like this too. Casing is a little different I do it in jars at 5 psi in PC for an hr and a half. Casing never usually get contams even left a jar on shelf for two weeks and it didnt go bad.





Or usually around 150 in and open test jar I have placed inside with remote thermometer I have rigged and sealed through my PC it welded to it to no worries there.


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InvisiblePirateSwazey
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110925 - 08/19/15 11:26 AM (8 years, 5 months ago)

Mold before first flush is always the result of bad spawn.


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22110940 - 08/19/15 11:29 AM (8 years, 5 months ago)

Quote:

PirateSwazey said:
Your contaminations are coming from your jars - not your substrate.

If you want clean spawn you need to step away from needles & inoculate w agar.

Also stop casing! No need in a bulk tub and it could also be part of your problem.





Aaaaahahahaaa there's that pic again. Freaky man. Love it. And u really think it coulb be my syringes? I always use different ones each time new and flame sterilized. I have 1 syringe for each jar flame sterile so I don't cross contam. And no casing? I don't.like to open to mist or crack the seal on my tubs till they fruit. Unthink without casing it could go dry?


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22110970 - 08/19/15 11:31 AM (8 years, 5 months ago)

Quote:

PirateSwazey said:
Mold before first flush is always the result of bad spawn.




Huh. So if it doesn't.show up in my jars that doesnt.mean it isn't there?


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Invisiblenatedawgnow
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110982 - 08/19/15 11:32 AM (8 years, 5 months ago)

If you are using pasteurized coir and getting
contams before the first flush, it is definitely
your spawn that is bad.

You need to get into agar so you can isolate clean growth.

Most contaminant molds are white until the get a little
fresh air, then they sporulate which makes them appear
blue or green.

Inside a jar, it can be difficcult to differentiate
between clean growth and contam growth.


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Offlinespacechildo
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110984 - 08/19/15 11:32 AM (8 years, 5 months ago)

1 syringe pr jar is NOT 1 drop per jar.. dont sterilize peat. and its still your grains.


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110986 - 08/19/15 11:33 AM (8 years, 5 months ago)

Also if I use more spawn than nessisary do u think it would colonize faster than the.mold could?


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InvisiblePirateSwazey
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22110994 - 08/19/15 11:35 AM (8 years, 5 months ago)

You don't mist tubs.. Just let them do their thing and stuff the holes with poly once they start pinning.

Set and forget! And nope I never ever ever case and always pull atleast 8 zips before I toss a sub out.

Yes your inoculation method is bad -- ask any serious grower they will tell you agar is the only way to go.

Quote:

megatacular said:
Quote:

PirateSwazey said:
Mold before first flush is always the result of bad spawn.




Huh. So if it doesn't.show up in my jars that doesnt.mean it isn't there?



Correct. Even after years of doing this and spawning close to 1000 tubs worth of spawn I still have trouble identifying some molds in spawn jars. But, working with a laminar flow hood and using agar to help insure clean cultures, I very rarely ever see a contamination until the 2nd flush is over when the mycelium has become weak.

Clean work makes the dream work :super:


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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Invisiblenatedawgnow
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111000 - 08/19/15 11:35 AM (8 years, 5 months ago)

Quote:

megatacular said:h
Also if I use more spawn than nessisary do u think it would colonize faster than the.mold could?



No. If there is mold in your spawn, it will eventually
rear its ugly head


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: natedawgnow]
    #22111031 - 08/19/15 11:38 AM (8 years, 5 months ago)

Quote:

natedawgnow said:
If you are using pasteurized coir and getting
contams before the first flush, it is definitely
your spawn that is bad.

You need to get into agar so you can isolate clean growth.

Most contaminant molds are white until the get a little
fresh air, then they sporulate which makes them appear
blue or green.

Inside a jar, it can be difficcult to differentiate
between clean growth and contam growth.





Wow man. I didn't.know that. That's very helpful. I will definaltly get into agar if that is possible to isolate just pure shroomage. How do I do that


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Invisiblenatedawgnow
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111064 - 08/19/15 11:43 AM (8 years, 5 months ago)

This is a great resource:

Stros cleaning up agar


--------------------


Edited by natedawgnow (08/19/15 11:45 AM)


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Offlinemegatacular
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111065 - 08/19/15 11:43 AM (8 years, 5 months ago)

Same to u swazey how much am I looking at here to do agar cultures there called right? What all do I need I wanna make a list then watch videos on it. Any links? I'll so anything to get my mushies man. I've tryed so hard so long.


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InvisiblePirateSwazey
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Re: help me please? i just want one successful flush. [Re: megatacular]
    #22111072 - 08/19/15 11:45 AM (8 years, 5 months ago)

www.mushroomvideos.com

There's an agar and petri section. Just buy the DVD, it's worth it.

I like premixed agar, plastic Petris & a whisky bottle to sterilize in.


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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InvisibleMad Season
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Re: help me please? i just want one successful flush. [Re: PirateSwazey]
    #22111086 - 08/19/15 11:49 AM (8 years, 5 months ago)

Not if you're running manure. In that case both under cooking and over cooking can fuck things up (I do 140-160, closer to 140 for 60 minutes. Definitely noticed a difference by bringing the times down from 90 minutes), as well as having the substrate too wet(I've never seen a thread saying sub was made too dry, because you can just mist it, but I have seen tons of subs made too wet). Although if you do the bulk prep right, and it still contaminates, it's always the spawn that's the problem.

Bad spawn isn't always the result of bad inoculant. I upped my pc times to 2+ hours, and have noticed the bacterial endospores really gone down. What do you use for filters?  Using a syringe instead of clean af agar is a lot more risky, and when it contaminates you have no idea what to blame.

What's always to blame is your mycelium shit the bed. Not your environment.
Quote:

Mad Season said:
Um weakened mycelium comes from a huge array of things. Including spawn. Always ALWAYS work on improving your prep procedures.

Quote:

Mad Season said:
There's lots of reasons. All of them stemming from the fact the mycelium shit the bed. And if it does sooner than it should, it's a problem that should be fixed. bacteria(more bacteria) through improperly sterilized grains(I've upped my pc times and changed grains too many times to count, but I'm loving oats, I do most qt jars 2 hours @15-20psi), improper pasteurization, improper hydration, hidden contams from improper procedures and there's many more stuff I bet you could improve on.

P.S. I didn't say toss them all. Just the ones with trich, and to always work on improving the conditions for the myc. The healthier the myc, the better things will be :thumbup:



Quote:

Mad Season said:
Quote:

hiperhiper said:
so what's the right procedure with grains?
I've checked the teks and nobody said anything about this. I didn't know I was not supposed to use syringe on grains. What is cleaning up syringe on agar? what does it mean?




http://www.shroomery.org/forums/showflat.php/Number/18431006
http://www.shroomery.org/forums/showflat.php/Number/18430998
http://www.shroomery.org/forums/showflat.php/Number/19208976 http://www.shroomery.org/forums/showflat.php/Number/21889950/page/1




--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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Edited by Mad Season (08/19/15 11:57 AM)


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