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OfflineAjahn Don
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Culture pic--What am I looking for?
    #22110370 - 08/19/15 09:39 AM (8 years, 5 months ago)

This is a picture of a KSSS 3rd transfer, 6 days ago. I don't have a program where I can draw lines on the pictures. What am I looking at?



I've been taking slices from the edges, where there are brighter white fans. Yes? No?

What's a sector?

I've seen a lot of pictures, and watched RR's videos. Not sure I'm seeing the same thing in mine, dig?

Any words of help would help. Thanks, always.

:shrug:


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OfflinetaGyo
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Re: Culture pic--What am I looking for? [Re: Ajahn Don]
    #22110396 - 08/19/15 09:49 AM (8 years, 5 months ago)

Keep transferring, I don't see sectors.

I worked with KSSS a lot, it all looks very fluffy:



Vs something like my PR:



Or APE:



I was told it was because it has a startling amount of genetics in it's code.

http://www.shroomery.org/forums/showflat.php/Number/18430998

That should explain a lot :thumbup:

Take your new cuts from the farthest edges. 1 o'clock and 10 o'clock look good.


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Edited by taGyo (08/19/15 09:50 AM)


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OfflineAjahn Don
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Re: Culture pic--What am I looking for? [Re: taGyo]
    #22110459 - 08/19/15 10:04 AM (8 years, 5 months ago)

Cool beans, mate! Great stuff. Kicks the ball a long way down the field for me. The white fluffy part. Got it. Thanks.

:smile:


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Re: Culture pic--What am I looking for? [Re: Ajahn Don]
    #22110916 - 08/19/15 11:24 AM (8 years, 5 months ago)

If that is from a print or syringe just grow it out already.. You don't want to isolate from MS, youll just be wasting your time. Always start from a clone!


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OfflinetaGyo
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Re: Culture pic--What am I looking for? [Re: PirateSwazey]
    #22112772 - 08/19/15 04:10 PM (8 years, 5 months ago)

Quote:

PirateSwazey said:
If that is from a print or syringe just grow it out already.. You don't want to isolate from MS, youll just be wasting your time. Always start from a clone!



:yesnod:


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OfflineAjahn Don
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Re: Culture pic--What am I looking for? [Re: taGyo]
    #22114189 - 08/19/15 09:03 PM (8 years, 5 months ago)

Hold it. Please excuse my ignorance, but this doesn't make nearly as much sense as it should. This is the third transfer of an initial drop of MS. Where do I get a clone if I have not grown anything? Where did the damn things come from in the first place? I know my syringes are from spores. You can see them floating around in the water. I dropped a single drop on a plate, grew that out and took what I hoped was the best mycelium, and did it two more times. This isn't good to do? What???

Tell you the truth, I'm not even sure what you mean by a clone. I have an idea, but it could be wrong. I took a piece of the inside of a stem of a BHT. Would that be a clone? At least I'm getting a lot of practice...at making mistakes.

Thanks.


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OfflinetaGyo
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Re: Culture pic--What am I looking for? [Re: Ajahn Don]
    #22114207 - 08/19/15 09:07 PM (8 years, 5 months ago)

Okay so basically what he's saying is you have a drop from an MS spore print there.

That MS spore could grow out to be just about anything. For instance, you could get a killer flush from that. OR you could get the worst pinset you'll ever see. Now as you transfer from this variable you're gradually bringing the variables down BUT you have no idea if you're going in the right direction or not because you haven't fruited anything.

So basically every transfer maybe you're transferring TO a killer flush or AWAY from a killer flush. So he, and I, suggest you fruit it and find out what you're dealing with.

A clone is basically a fruit you crack in half and take an inner tissue sample from and put it on agar. This fruit is your base for your isolations. If you cut away from this and fruit that and you get less shrooms then you know you took the wrong sector. If you take another and get the great pinset you were looking for then you know you got the right sector and can keep going down and down from there. This way you can get to a monoculture that you KNOW is potent and fruits well.

Clone clusters for more yield and big mushrooms for big mushrooms :thumbup:


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OfflineAjahn Don
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Re: Culture pic--What am I looking for? [Re: taGyo]
    #22114299 - 08/19/15 09:30 PM (8 years, 5 months ago)

Now THAT is an explanation! Pardon me while I go back and read it two or three more times before I start to get it.  :lol:

This is going to take some time, isn't it? Fortunately, I'm hooked. And I haven't even consumed any yet. That should be FRIDAY! yeah, baby!

Okay, so I take a piece from the inside, grow it out, and then fruit it. Do you save the culture from which you fruited? Would all the fruits from this grow be consistent? Growth and power. (I got to take better notes!)

Thanks, man. Hope this is helping others, too.

:wink:


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Re: Culture pic--What am I looking for? [Re: PirateSwazey]
    #22114321 - 08/19/15 09:36 PM (8 years, 5 months ago)

Quote:

PirateSwazey said:
If that is from a print or syringe just grow it out already.. You don't want to isolate from MS, youll just be wasting your time. Always start from a clone!



i'd have to strongly disagree, i find it the best way to go myself:shrug: cloning kicks ass but is just as much of  a hit or miss


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OfflinetaGyo
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Re: Culture pic--What am I looking for? [Re: Ajahn Don]
    #22114396 - 08/19/15 09:57 PM (8 years, 5 months ago)

Quote:

Ajahn Don said:
Now THAT is an explanation! Pardon me while I go back and read it two or three more times before I start to get it.  :lol:

This is going to take some time, isn't it? Fortunately, I'm hooked. And I haven't even consumed any yet. That should be FRIDAY! yeah, baby!

Okay, so I take a piece from the inside, grow it out, and then fruit it. Do you save the culture from which you fruited? Would all the fruits from this grow be consistent? Growth and power. (I got to take better notes!)

Thanks, man. Hope this is helping others, too.

:wink:



In theory yes, cloning a fruit will give you a much less random genetic pool then an MS syringe. Yes, keep the culture you fruit from. Best to do multiple transfers from your donor and then an additional transfer and then fruit those third generation.

If it's a clone I make one transfer and then fruit it.

Hey Cron, correct me if I'm wrong but you shouldn't clone a clone because of senescence, correct?


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OfflinecronicrFacebook
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Re: Culture pic--What am I looking for? [Re: taGyo]
    #22114408 - 08/19/15 09:58 PM (8 years, 5 months ago)

yes go back to the plate:super:


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OfflinetaGyo
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Re: Culture pic--What am I looking for? [Re: cronicr]
    #22114414 - 08/19/15 09:59 PM (8 years, 5 months ago)

:rockon:

For OP, senescence is when the myc has expanded a lot and is "tired". It'll grow much slower or even stall completely.


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OfflinecronicrFacebook
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Re: Culture pic--What am I looking for? [Re: cronicr]
    #22114460 - 08/19/15 10:11 PM (8 years, 5 months ago)

Quote:

cronicr said:
Quote:

PirateSwazey said:
If that is from a print or syringe just grow it out already.. You don't want to isolate from MS, youll just be wasting your time. Always start from a clone!



i'd have to strongly disagree, i find it the best way to go myself:shrug: cloning kicks ass but is just as much of  a hit or miss



IMO cloning is never better the isolating because it still take s a bunch of transfers, there has already been a lot of cell division taken place, it's still hit or miss, more then one strain may be present and isolating may take away what made it work in the first place


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I'm tired do me a favor


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OfflinetaGyo
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Re: Culture pic--What am I looking for? [Re: cronicr]
    #22114461 - 08/19/15 10:11 PM (8 years, 5 months ago)

:nodofunderstanding:


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Re: Culture pic--What am I looking for? [Re: Ajahn Don]
    #22114490 - 08/19/15 10:18 PM (8 years, 5 months ago)

Quote:

Ajahn Don said:
Hold it. Please excuse my ignorance, but this doesn't make nearly as much sense as it should. This is the third transfer of an initial drop of MS. Where do I get a clone if I have not grown anything? Where did the damn things come from in the first place? I know my syringes are from spores. You can see them floating around in the water. I dropped a single drop on a plate, grew that out and took what I hoped was the best mycelium, and did it two more times. This isn't good to do? What???

Tell you the truth, I'm not even sure what you mean by a clone. I have an idea, but it could be wrong. I took a piece of the inside of a stem of a BHT. Would that be a clone? At least I'm getting a lot of practice...at making mistakes.

Thanks.



just keep transferring, i also like to keep my plates i made transfers from till they pin... quick clone:cool:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisibleblindingleaf
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Re: Culture pic--What am I looking for? [Re: cronicr]
    #22114509 - 08/19/15 10:23 PM (8 years, 5 months ago)

Quote:

IMO cloning is never better the isolating because it still take s a bunch of transfers,




Quote:

just keep transferring, i also like to keep my plates i made transfers from till they pin... quick clone:cool:




what kind of differences do you see between the two?


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OfflinetaGyo
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Re: Culture pic--What am I looking for? [Re: blindingleaf]
    #22114515 - 08/19/15 10:24 PM (8 years, 5 months ago)

BL is here too?

:yaysugar:


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Re: Culture pic--What am I looking for? [Re: blindingleaf]
    #22114521 - 08/19/15 10:26 PM (8 years, 5 months ago)

Quote:

blindingleaf said:
Quote:

IMO cloning is never better the isolating because it still take s a bunch of transfers,




Quote:

just keep transferring, i also like to keep my plates i made transfers from till they pin... quick clone:cool:




what kind of differences do you see between the two?



the biggest being longevity but also an isolate is easier to test potency and blah blah blah


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisibleblindingleaf
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Re: Culture pic--What am I looking for? [Re: taGyo]
    #22114522 - 08/19/15 10:26 PM (8 years, 5 months ago)

i've been running MS most of this summer.  just curious


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Re: Culture pic--What am I looking for? [Re: blindingleaf]
    #22114751 - 08/19/15 11:26 PM (8 years, 5 months ago)

Guise.. Let's get real for a minute here now.. These are cubes were talking about after all. The mother fuckers are like goddamn weeds and once you know what you're doing there's not a whole lot you can do to fuck it up... If you want to grow more u don't need to clock in tons of time on trying out strains.. Just boil more water for coir, make more spawn and drill more tubs. That's always been my philosophy anyways :rofl:


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OfflineAjahn Don
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Re: Culture pic--What am I looking for? [Re: PirateSwazey]
    #22118049 - 08/20/15 06:40 PM (8 years, 5 months ago)

Quote:

PirateSwazey said:
Guise.. Let's get real for a minute here now.. These are cubes were talking about after all. The mother fuckers are like goddamn weeds and once you know what you're doing there's not a whole lot you can do to fuck it up... If you want to grow more u don't need to clock in tons of time on trying out strains.. Just boil more water for coir, make more spawn and drill more tubs. That's always been my philosophy anyways :rofl:




This reminds me of a previous life I had. I was one of the guys who came out to this god-forsaken desert (which I love and would never leave) during the poker boom. I spent a lot of time on the definitive forum for poker players. My best friend and great poker coach (who got me into meditation) said, "The things we argue about the most are the closest decisions, which rarely affect the bottom line." Are you guys talking about close decisions, and Pirate Swazey comes along and says it's just a cube? I assumed there was a noticeable difference in development and potency. Pirate Sway, are you saying there is not enough difference to matter between MS and monoculture? (Not sure I buy that any strain of cannabis is superior; a weed is a weed.)

I think I'm still a freshman who has stumbled on a master's class.

Thanks for the good stuff. After three months of reading, I haven't found any of this in one place, and Search has never been good at this kind of stuff. You guys are high-class, grade A enthusiasts. Peace.

:woot:

PS--I get the feeling that if I went back to taGyo's first threads, I'd get a fast-track degree in clandestine shromery.  lol


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Re: Culture pic--What am I looking for? [Re: Ajahn Don]
    #22119607 - 08/21/15 12:25 AM (8 years, 5 months ago)

Pirate is awesome and rocks a good point....i jst like to nit pick lol


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Re: Culture pic--What am I looking for? [Re: cronicr]
    #22119655 - 08/21/15 12:42 AM (8 years, 5 months ago)

I love clones for clones! But for isolating, it makes sense to isolate while they're young and ripe


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OfflineAjahn Don
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Re: Culture pic--What am I looking for? [Re: Mad Season]
    #22130533 - 08/23/15 11:40 AM (8 years, 5 months ago)

From a piece of GT. Sorry for the picture quality. My phone focuses on the lid, rather than the subject.

At three o'clock, there is a little tiny tree. Can you see it well enough to give me some education on it? It clearly is different from the rest. Is this something to grab right away and culture separately?

Thanks.




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Re: Culture pic--What am I looking for? [Re: Ajahn Don]
    #22159957 - 08/29/15 09:59 AM (8 years, 5 months ago)

More pics and questions.

These are from a KSSS pin. Should I take the long ropy threads or the more tightly packed?



This is a BHT clone, 15 days out. (that's not bacteria, but sloppy agar prep/food coloring.) Should I separate the two parts? What's going on here?



This is the second transfer of another KSSS pin. 5 days out. Should I separate into three dishes, or wait?



Thanks for your help. I don't know what I'm looking at, yet. We're looking for the strongest growth, yes? More rhysome is better than tomentose?

:takingnotes:


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Re: Culture pic--What am I looking for? [Re: Ajahn Don]
    #22159970 - 08/29/15 10:02 AM (8 years, 5 months ago)

Farthest along ropy section,

I see a lot of bacteria in that BHT Jar (What does BHT stand for btw?). I would transfer immediately from the farthest along edge. It looks like you have two different genetics in their and they're not playing nice.

Wait for that last transfer of KSSS for it to grow a little and see if it's a clean transfer. Sometimes bacteria shows up like a day or two after.


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Re: Culture pic--What am I looking for? [Re: taGyo]
    #22159977 - 08/29/15 10:03 AM (8 years, 5 months ago)

ban hua thanon, that's not how it's spelled but some shit like that:lmafo:


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I'm tired do me a favor


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Re: Culture pic--What am I looking for? [Re: cronicr]
    #22159979 - 08/29/15 10:04 AM (8 years, 5 months ago)

Like the tenth thread I've seen about BHT and I'm always like ???

Thanks Cron :lol:


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OfflineAjahn Don
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Re: Culture pic--What am I looking for? [Re: taGyo]
    #22160735 - 08/29/15 01:13 PM (8 years, 5 months ago)

Thanks, guys. Not bac. Just bad agar tek.


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