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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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How long do I have to inoculate once ive pressure cooked...
#22110234 - 08/19/15 08:51 AM (8 years, 5 months ago) |
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The reason i ask..., well, i guess i had some spores that went bad. they sat in my fridge for atleast a few months or longer. i shot up a total of 120 jars. only about 20-30 or so germinated and colonized. i still see no signs of contamination in them tho. my new spores arrived recently. would it be worth it to shoot them up with the new syringes? thx
-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: How long do I have to inoculate once ive pressure cooked... [Re: HiLIFE]
#22110240 - 08/19/15 08:53 AM (8 years, 5 months ago) |
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Um.. you should look into agar.. unless you want to keep buying and shooting spores.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: How long do I have to inoculate once ive pressure cooked... [Re: Mad Season]
#22110252 - 08/19/15 08:57 AM (8 years, 5 months ago) |
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start over or at least re-sterilize the jars rather than re-inoculate them! spores dont go bad in the fridge over a cpl months, something else was wrong.
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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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Re: How long do I have to inoculate once ive pressure cooked... [Re: spacechildo]
#22110280 - 08/19/15 09:07 AM (8 years, 5 months ago) |
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Quote:
spacechildo said: start over or at least re-sterilize the jars rather than re-inoculate them! spores dont go bad in the fridge over a cpl months, something else was wrong.
This, and I'll add that grain is cheap compared to time in a day... are you really saving anything if you re-sterilize, waste more spores, and end up dumping the jars anyway?
I had a syringe for over 5 years, stored in a shed with no environmental controls, and it had 100% germination, so space is right, there's another problem. Have any pics? Maybe we can help figure out what it was.
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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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Re: How long do I have to inoculate once ive pressure cooked... [Re: Srirachi]
#22111091 - 08/19/15 11:50 AM (8 years, 5 months ago) |
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Planing to start working with agar soon. Didnt know you could just resterilize the jars though. I think ill try that. I know my grain prep was fine. And I use SHIP's and permanent filters on my lids. Something was wrong with the syringes. It may have been longer then a few months. But something had to be up with those syringes.
-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: How long do I have to inoculate once ive pressure cooked... [Re: HiLIFE]
#22112942 - 08/19/15 04:38 PM (8 years, 5 months ago) |
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No need for the SHIP, you can just crack the jar lid inside your SAB, it's a lot easier and lets you practice your sterile techniques!
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: How long do I have to inoculate once ive pressure cooked... [Re: Psilosoulful]
#22115016 - 08/20/15 12:34 AM (8 years, 5 months ago) |
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Like the other guys said. Do not reshoot pf jars never stick a clean needle in an unknown. I would cook those jars again and then knock them up. Like others said, the spores should not have gone bad. I am currently using 5 year old, half used syringes from sporeworks. On that note I am also using 5 year old culture syringes from spore works.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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keeno
enthusiast



Registered: 06/01/11
Posts: 2,679
Loc: UK
Last seen: 14 days, 10 hours
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Re: How long do I have to inoculate once ive pressure cooked... [Re: tahoe]
#22115280 - 08/20/15 03:57 AM (8 years, 5 months ago) |
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are you allowed to mention vendors by name?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: How long do I have to inoculate once ive pressure cooked... [Re: keeno]
#22115287 - 08/20/15 04:02 AM (8 years, 5 months ago) |
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You are not.
tahoe's been around since before that was a rule I believe, probably dipped out for that board meeting in particular. 
http://www.shroomery.org/forums/showflat.php/Number/14960167
I'd just edit it so a mod doesn't get involved.
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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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Re: How long do I have to inoculate once ive pressure cooked... [Re: Inocuole]
#22115872 - 08/20/15 09:21 AM (8 years, 5 months ago) |
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It just doesn't make sense tho. Ive been doing this for 6 years. Never had jars that I inoculated just not germinate in such a high number. I use 1cc per jar like I always have. something went wrong, and im not sure what.
And I prefer SHIPs over having to work inside a SAB. Makes life much easier.
-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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Re: How long do I have to inoculate once ive pressure cooked... [Re: HiLIFE]
#22115879 - 08/20/15 09:23 AM (8 years, 5 months ago) |
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Maybe I just didn't shake them up enough and break up the spores so all I was shooting was water into some. But i honestly dont think that is it either. So confused.
-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: How long do I have to inoculate once ive pressure cooked... [Re: HiLIFE]
#22117657 - 08/20/15 05:11 PM (8 years, 5 months ago) |
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If you used spores then that's enough to explain why you aren't having consistent results. Agar or bust.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: How long do I have to inoculate once ive pressure cooked... [Re: keeno]
#22117739 - 08/20/15 05:28 PM (8 years, 5 months ago) |
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Quote:
keeno said: are you allowed to mention vendors by name? 
When you mention growing their edible cultures (like Tahoe did) then yes.
Don't re-cook the jars. Start over. Sounds like it was definitely your syringe.
Open some of the ones that did "nothing" and see what they smell like. I'd bet $1 that they're bacterial. Probably caught it from your fridge.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: How long do I have to inoculate once ive pressure cooked... [Re: 36fuckin5] 1
#22117748 - 08/20/15 05:30 PM (8 years, 5 months ago) |
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Not everybody in the actives forum is gonna assume he means edibles though, I didn't know about it based on the language used. 
I mean, it's not my business anyway, not like I'm gonna blow any whistles for shit like that.
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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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Re: How long do I have to inoculate once ive pressure cooked... [Re: 36fuckin5]
#22139765 - 08/25/15 08:36 AM (8 years, 5 months ago) |
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That wouldn't make sense then that some of the same batches germinated and fully colonized. And others contaminated with bacterial. If the whole syringe was contamed, they would all end up bad. Funny thing is. I have opened many that didn't colonize, and they smell just fine. Im gonna re-shoot 10 that I re-cooked with brand new syringes just to see what might happen. Agar is in my near future. Just a little intimidated I guess.
-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: How long do I have to inoculate once ive pressure cooked... [Re: HiLIFE]
#22139783 - 08/25/15 08:42 AM (8 years, 5 months ago) |
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Quote:
HiLIFE said: Agar is in my near future. Just a little intimidated I guess.
It's easier than you think and your success rates will increase greatly. MS>grain is always a risky move. You never get a completely clean syringe, so why take the contam risk shooting the spores into your grains?
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: How long do I have to inoculate once ive pressure cooked... [Re: HiLIFE]
#22139877 - 08/25/15 09:17 AM (8 years, 5 months ago) |
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Quote:
HiLIFE said: That wouldn't make sense then that some of the same batches germinated and fully colonized. And others contaminated with bacterial. If the whole syringe was contamed, they would all end up bad. Funny thing is. I have opened many that didn't colonize, and they smell just fine.
When you dilute spores in water, the contams get diluted too. That's the entire point of putting them in a syringe. That way, when you put them under a microscope (because they REALLY ARE made for microscopy in the first place) you can see the spores and make a positive ID.
Quote:
Im gonna re-shoot 10 that I re-cooked
That's a bad idea. Never re-cook jars. It's another contam vector.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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Re: How long do I have to inoculate once ive pressure cooked... [Re: Psilosoulful]
#22139943 - 08/25/15 09:40 AM (8 years, 5 months ago) |
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care to share a Tek ideal for a first timer. ive seen so many!
-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: How long do I have to inoculate once ive pressure cooked... [Re: HiLIFE]
#22139948 - 08/25/15 09:42 AM (8 years, 5 months ago) |
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HiLIFE
Advanced Mycologist


Registered: 11/16/11
Posts: 132
Loc: Denver, Colorado
Last seen: 3 years, 3 months
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Re: How long do I have to inoculate once ive pressure cooked... [Re: 36fuckin5]
#22139951 - 08/25/15 09:42 AM (8 years, 5 months ago) |
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well, i smelled all of the jars that didnt ever germinate, and none of them were contaminated. they all smelled like rye grain. the damage is done though. i have two pressure cookers and i pressure cooked 20 jars and already shot them up. 10 bucks a syringe, its a 20 dollar gamble. hoping for the best, preparing for the worst though. thanks for the 411. learn something new everyday i decide to read/post on here. you guys are awesome.
-------------------- "Out beyond ideas of wrongdoing and rightdoing there is a field. I'll meet you there. When the soul lies down in that grass the world is too full to talk about." - Rumi
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