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BoomRad
HtemToohs


Registered: 07/22/15
Posts: 498
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Wbs storage
#22108292 - 08/18/15 07:47 PM (8 years, 5 months ago) |
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Hey, I am new here, however I am not a beginner. I have been using the shroomery for a very long time, and have learned a ton by just searching posts.. However I have just recently sterilized 36 quarts of wbs for spawn and I am wondering how soon I have to inoculate it. Typically I knock my spawn up asap, but I am getting ready to go on vacation and am wondering if I am going to be ok without knocking them up before I leave.. Another question I have is about isolation; when I have a perfect specimen I choose to clone, can I use a piece of the tissue and just put it into a jar of wbs? Or must I make a spore print and then use that to make syringe etc etc etc??? Thanks in advance and sorry for the long post!
-------------------- Gnome saying
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wbs storage [Re: BoomRad]
#22108297 - 08/18/15 07:48 PM (8 years, 5 months ago) |
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you could be ok to wait a week or more i'd personelly give it a quick pc run when i get back but i'm paranoid, for cloning clone to agar, grains can be tricky and spores are very random
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Wbs storage [Re: cronicr]
#22108320 - 08/18/15 07:55 PM (8 years, 5 months ago) |
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I've let bags sit for 2 months before knocking them up.. the jars will be fine!
As for cloning you need to put the tissue to agar before inoculating grain with it.
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BoomRad
HtemToohs


Registered: 07/22/15
Posts: 498
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Sweet; thanks guys this is pretty sweet how quick that works!! You guys are awesome!! Now as far as cloning goes agar is something I haven't tried do I need to start a new thread to ask about that? I know there's info about it out there but what I have read in other posts doesn't make very much sense to me... About to harvest my mono tub, thanks again for your help
-------------------- Gnome saying
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Wbs storage [Re: BoomRad]
#22108419 - 08/18/15 08:20 PM (8 years, 5 months ago) |
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Go to www.mushroomvideos.com -- there's a quick run thru under agar & petri dishes.
If you don't have the video already I would buy it, it's worth every penny!
I think it only costs like $12, but you might have to go thru the shroomery to get that price.
Anyways here are some more helpful links:
Stro's cleaning and isolating http://www.shroomery.org/forums/showflat.php/Number/18430998#18430998
Pasty's newb agar http://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1/nt/123
Cloning w/ Franklin http://www.shroomery.org/forums/showflat.php/Number/17784996#17784996
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BoomRad
HtemToohs


Registered: 07/22/15
Posts: 498
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Thanks a lot, greatly appreciated
-------------------- Gnome saying
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megatacular
professor procrastinate


Registered: 08/18/15
Posts: 183
Loc: Shittown Texas
Last seen: 8 years, 4 months
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Re: Wbs storage [Re: BoomRad]
#22108579 - 08/18/15 08:52 PM (8 years, 5 months ago) |
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Omg there it is again. That wacked out motion pic of uncle Sam. I'm so scared looking at that thing but he's everywhere I can't stop. AaaaĆ aaaĆ aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaahhhhhhhhhhhhh
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megatacular
professor procrastinate


Registered: 08/18/15
Posts: 183
Loc: Shittown Texas
Last seen: 8 years, 4 months
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Oh yeah I know its not helping much but I can't wait to start my first grow for cloning as well I wanna make all different kinds of clones. Lc. Agar. Prints I want a storage bank of every mushie I can get my hands on and bring to life. So I'm doing lots of research on the most trusted teks in doing so but am taking any advise along the way as well. Good luck to you fellow shroomer and pleasent trips.
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