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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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clone question
#22108039 - 08/18/15 06:38 PM (8 years, 5 months ago) |
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I have an Pasty agar dish that has been sitting for weeks unattended. today I found to my shock and awe two baby mushrooms growing on the medium. My question is: Can I pick one of those mushrooms--with some sterilized tweezers-- and drop it into a fresh agar dish whole for the purpose of cloning it? I have searched for this both here and on google--nada.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Yes I've heard people doing this with pins. Not sure about baby mushrooms tho. It would be best to split the stem in a SAB and take a tissue sample and the transfer that to the new agar dish.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
Psilosoulful said: It would be best to split the stem in a SAB and take a tissue sample and the transfer that to the new agar dish.
no need to it was in a sterile enviroment
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: clone question [Re: cronicr]
#22108150 - 08/18/15 07:08 PM (8 years, 5 months ago) |
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Quote:
cronicr said:
Quote:
Psilosoulful said: It would be best to split the stem in a SAB and take a tissue sample and the transfer that to the new agar dish.
no need to it was in a sterile enviroment
OP only said he was gonna use sterilized tweezers, he never said anything about doing the procedure in a SAB..
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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well he more then likely did his plates in one
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: clone question [Re: cronicr]
#22108260 - 08/18/15 07:39 PM (8 years, 5 months ago) |
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Don't do it man, I saw this movie with arnold schwarzenegger, and like...
Just kidding, snag them pins n chuck'm on a new plate.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: clone question [Re: cronicr]
#22110169 - 08/19/15 08:21 AM (8 years, 5 months ago) |
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Quote:
cronicr said: well he more then likely did his plates in one
Yes, I did do the Pasty dishes in a SAB. And ,yes, I intend to proceed with this operation in the same venue.
It is because the little mushroom--a pretty plucky guy, if you ask me--grew up in a sterile environment that I think I can get away with this maneuver. I will revive this thread with pics if anything results.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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