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OfflineMajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
Preparing 4 LC jars
    #22106250 - 08/18/15 11:14 AM (8 years, 5 months ago)

One out of four busted, the bottom shattered because it fell over during the process :kummeli:

Fuck! God fucking dammit!  :thatistroubling:  :whatwhatwhat:

Its okay though I still have 3 good ones to start my Gymnopilus aeruginosus
:yeahthatsright:


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OfflineMajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
Re: Preparing 4 LC jars [Re: MajickMuffin]
    #22106330 - 08/18/15 11:30 AM (8 years, 5 months ago)

To add onto this, im working with a Spore Print, so im going to have to briefly open the caps of the jars to put the spores in.

Anyone have any information or experience regarding that?


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Preparing 4 LC jars [Re: MajickMuffin]
    #22106408 - 08/18/15 11:46 AM (8 years, 5 months ago)

It's gonna end bad. Go to agar first.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlineshellzenone
Zen
Male User Gallery


Registered: 12/11/14
Posts: 688
Last seen: 1 year, 8 months
Re: Preparing 4 LC jars [Re: MajickMuffin]
    #22106474 - 08/18/15 11:56 AM (8 years, 5 months ago)

It can be done, however, you have to make sure the spore print is not contaminated. It's hard to tell in a lc.


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Offlinetetherface
get in where you fit in
Male User Gallery


Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
Re: Preparing 4 LC jars [Re: shellzenone]
    #22106495 - 08/18/15 12:03 PM (8 years, 5 months ago)

I have never had success with spores to lc listen to 36fuckin5 its gonna be bad......


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Invisiblenatedawgnow
Rocky mountain hood rat
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Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Preparing 4 LC jars [Re: MajickMuffin]
    #22106505 - 08/18/15 12:05 PM (8 years, 5 months ago)

Quote:

MajickMuffin said:
To add onto this, im working with a Spore Print, so im going to have to briefly open the caps of the jars to put the spores in.

Anyone have any information or experience regarding that?




Agar first!!!

Especially if this is a wild print you are working with.
If so, it is almost guaranteed to be contaminated.


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InvisibleJuiceh
Dabbing All Day
 User Gallery


Registered: 09/25/12
Posts: 3,208
Re: Preparing 4 LC jars [Re: 36fuckin5]
    #22106507 - 08/18/15 12:06 PM (8 years, 5 months ago)

Quote:

36fuckin5 said:
It's gonna end bad. Go to agar first.



:whathesaid:

Print to LC is a bad way to go, way too much risk. Get clean germination of the spores on agar first, then transfer a wedge of clean mycelium to the LC.


--------------------
Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


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OfflineMajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
Re: Preparing 4 LC jars [Re: Juiceh]
    #22106725 - 08/18/15 01:16 PM (8 years, 5 months ago)

What if the print was made from cultivated fruits in a sterile environment?


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Preparing 4 LC jars [Re: MajickMuffin]
    #22106753 - 08/18/15 01:23 PM (8 years, 5 months ago)

It wasn't a sterile environment.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineMajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
Re: Preparing 4 LC jars [Re: 36fuckin5]
    #22106757 - 08/18/15 01:25 PM (8 years, 5 months ago)

Can someone give me a link for the agar shit?

Thanks guys


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OfflineGreen Bastard
Stranger
Male User Gallery
Registered: 02/26/14
Posts: 199
Loc: Canada Flag
Last seen: 3 years, 8 months
Re: Preparing 4 LC jars [Re: MajickMuffin]
    #22106791 - 08/18/15 01:38 PM (8 years, 5 months ago)

Fruits cultivated in a sterile environment? Highly doubtful...


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InvisibleJuiceh
Dabbing All Day
 User Gallery


Registered: 09/25/12
Posts: 3,208
Re: Preparing 4 LC jars [Re: Green Bastard]
    #22106859 - 08/18/15 01:59 PM (8 years, 5 months ago)

How did you have a jar fall over in the PC?


Plates:
http://www.ebay.com/itm/Sterile-Polystyrene-Petri-Dish-Plate-100x15-mm-500-cs-/111668743267?pt=LH_DefaultDomain_0&hash=item19fff9cc63

Agar Agar:
http://out-grow.com/culture-media-isolation-tools-c-10/agar-agar-powder-p-114.html

If you have grain water from some soaked grains, that's all you need in terms of ingredients. If you don't have any grain water, then you should also grab some light malt extract.
http://out-grow.com/culture-media-isolation-tools-c-10/8-ounces-of-light-malt-extract-p-79.html

You need a SAB\flowhood, and empty liquor bottle\suitable agar vessel, a scalpel\xacto knife, a torch for flame sterilization and then some cling wrap or parafilm to wrap the plates in, then you're set.

bodhisatta's tek;
https://www.shroomery.org/forums/showflat.php/Number/21922023/fpart/1


--------------------
Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


Edited by Juiceh (08/18/15 02:06 PM)


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Preparing 4 LC jars [Re: MajickMuffin]
    #22106884 - 08/18/15 02:06 PM (8 years, 5 months ago)

Quote:

MajickMuffin said:
Can someone give me a link for the agar shit?

Thanks guys




http://bfy.tw/rhg


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlineblackout
 User Gallery

Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
Trusted Cultivator
Re: Preparing 4 LC jars [Re: Green Bastard]
    #22107044 - 08/18/15 02:52 PM (8 years, 5 months ago)

Quote:

Green Bastard said:
Fruits cultivated in a sterile environment? Highly doubtful...



The word "sterile" does nothing but cause arguments, you could sterilize grains at 60psi for a year and someone would question the sterility. Some would argue that a sterile environment is an impossibility so the discussion ends there.

You can grow and have mushrooms drop spores in unusually clean ways though. Transfer supposedly clean growth to supposedly sterile subs in small jars and let them fruit invitro. When the shroom matures tip the jar on its side and let spores drop, now inject sterile water and draw out spores. So the jar is never opened once. This liquid may mix with substrate and so have nutrients which mix with the spores. There may be methods to overcome this but if you are not a vendor it might not bother you.

I suspect some of the vendors are using techniques unknown to the general public. Some people talk as though they are all pretty much identical.


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Preparing 4 LC jars [Re: 36fuckin5]
    #22107132 - 08/18/15 03:12 PM (8 years, 5 months ago)

Quote:

36fuckin5 said:
Quote:

MajickMuffin said:
Can someone give me a link for the agar shit?

Thanks guys




http://bfy.tw/rhg



:lol:


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


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OfflineMajickMuffin
Edible Cult


Registered: 05/28/14
Posts: 4,345
Loc: North
Last seen: 2 years, 8 months
Re: Preparing 4 LC jars [Re: 36fuckin5]
    #22107140 - 08/18/15 03:15 PM (8 years, 5 months ago)

Quote:

36fuckin5 said:
Quote:

MajickMuffin said:
Can someone give me a link for the agar shit?

Thanks guys




http://bfy.tw/rhg



:kummeli:


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InvisibleFantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc: Flag
Re: Preparing 4 LC jars [Re: MajickMuffin]
    #22107182 - 08/18/15 03:23 PM (8 years, 5 months ago)

Come on now...
Was tht necessary?
lol


--------------------
Giving is all we have, for we're just grateful to be alive

:kratom:


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InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Preparing 4 LC jars [Re: MajickMuffin]
    #22107192 - 08/18/15 03:26 PM (8 years, 5 months ago)

Quote:

MajickMuffin said:
Can someone give me a link for the agar shit?

Thanks guys



Quote:

Mad Season said:
Quote:

hiperhiper said:
so what's the right procedure with grains?
I've checked the teks and nobody said anything about this. I didn't know I was not supposed to use syringe on grains. What is cleaning up syringe on agar? what does it mean?




http://www.shroomery.org/forums/showflat.php/Number/18431006
http://www.shroomery.org/forums/showflat.php/Number/18430998
http://www.shroomery.org/forums/showflat.php/Number/19208976 http://www.shroomery.org/forums/showflat.php/Number/21889950/page/1



Also it's still on! The gym grow challenge haha


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (08/18/15 03:32 PM)


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Invisibleblindingleaf
blue collar underworld
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Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Preparing 4 LC jars [Re: Mad Season]
    #22107225 - 08/18/15 03:34 PM (8 years, 5 months ago)

Quote:

You can grow and have mushrooms drop spores in unusually clean ways though.



:bigyesnod:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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