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mezzy jeb
Skywalker


Registered: 07/28/12
Posts: 291
Last seen: 2 years, 4 months
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Contamination central
#22106215 - 08/18/15 11:04 AM (8 years, 5 months ago) |
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Hey, I still consider myself a novice grower even though I've had several successful monotub grows.
I'm hitting a spot of trouble, I moved into a new apartment and I've tried two grows, and now either my jars contaminate or my tubs contaminate after the 1st flush.
I haven't varied my sterile technique, in fact in the most recent grow I used a properly constructed SAB for the first time, so if anything my success rate should have been virtually assured.
So I'm wondering if it's possible that my apartment is horribly dirty. When I moved in there was mouse shit everywhere, and it's a very old building... you get the idea. I've heard people talking about how some rooms are just too dirty to do work in, but I don't know if this is verified or widely accepted.
So, is it possible that my dirty apartment is screwing me up, or is the fault in my sterile technique, despite having far less contam problems in the place I lived before?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Contamination central [Re: mezzy jeb] 1
#22106252 - 08/18/15 11:15 AM (8 years, 5 months ago) |
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Not the apartment. I do my sterile work in a carpeted room with a cat and litter box. It's techniques, practice, and dexterity
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mezzy jeb
Skywalker


Registered: 07/28/12
Posts: 291
Last seen: 2 years, 4 months
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Re: Contamination central [Re: bodhisatta]
#22106281 - 08/18/15 11:21 AM (8 years, 5 months ago) |
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Then why do people say stuff like "Don't open contaminated jars inside! Get them outside immediately - you don't want a spore-load in your place unless you don't plan on growing there anymore."
I'm sure you agree it's not a good idea to open contaminants inside, so it's not just technique and dexterity. Environment plays a role. Even guys with perfect technique and $500 laminar flow hoods don't open contams in their places. For all I know this apartment has a crazy amount of mold spores; many more than my old place, as if I had opened contaminated jars in it. Surely environment plays a part?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Contamination central [Re: mezzy jeb]
#22106298 - 08/18/15 11:25 AM (8 years, 5 months ago) |
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With A SAB you can do sterile work in a garbage heap. With a FH you really should have a damn clean work area, and let it run some time. Contamination in your environment never helps but it won't ruin you. Especially using a SAB. It takes much greater sterile technique to use a hood
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Contamination central [Re: mezzy jeb]
#22106308 - 08/18/15 11:28 AM (8 years, 5 months ago) |
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It does if your substrates mycelium is weakened. Lots of reasons can do it. Subs too wet, bacteria/dirty spawn, bad pasteurization, etc.
If you can get your prep work perfect, and using agar make everything right up until spawning time clean as a whistle, the amount of spores in your home will not effect your healthy mycelium. Although being perfect is a lot harder than it sounds. Obviously the dirty jar slips by us, and if you had a low concentration of mold spores, the chances of it contaminating will be diminished.
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tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
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Re: Contamination central [Re: bodhisatta]
#22106464 - 08/18/15 11:54 AM (8 years, 5 months ago) |
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I stayed with my brother in law one time and I had a horrible time keeping my shit clean I had maybe a 25% success rate but this guy said "my dogs a house dog" and trained it to piss and shit in the basement so there was moldy shit just a brewin in the basement it was so fuckin gross so I believe its not detrimental to success but it sure doesn't help your gonna have to concentrate on getting your home air very still and keep your movements slow in your SAB to not stir up any nasties
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 20 hours, 14 minutes
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Re: Contamination central [Re: mezzy jeb]
#22106492 - 08/18/15 12:01 PM (8 years, 5 months ago) |
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Quote:
mezzy jeb said: Then why do people say stuff like "Don't open contaminated jars inside! Get them outside immediately - you don't want a spore-load in your place unless you don't plan on growing there anymore."
I'm sure you agree it's not a good idea to open contaminants inside, so it's not just technique and dexterity. Environment plays a role. Even guys with perfect technique and $500 laminar flow hoods don't open contams in their places. For all I know this apartment has a crazy amount of mold spores; many more than my old place, as if I had opened contaminated jars in it. Surely environment plays a part?
One thing that is certain is a lot molds will continually release spores into the air until you remove the substrate. It's also fair to assume that if you dump a mold filled jar out indoors you're going release a lot mold spores in the process. The spores would be pretty much be out of the air within in hour but you still have spores collecting surfaces that can be stirred up or dragged around until they're all finally removed.
If you clean the place well the spore-load will eventually revert to a fraction of what it is outdoors assuming there's no active mold sources indoors. BTW, if it's higher outdoors it will usually be higher indoors as well.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Contamination central [Re: Kizzle]
#22106549 - 08/18/15 12:17 PM (8 years, 5 months ago) |
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That and an outside dump is always good for safety when you have no clue what contamination you're dumping. Some spores are nasty. Outdoors you're not going to get them all in your lungs unless you huff it
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Contamination central [Re: bodhisatta]
#22106620 - 08/18/15 12:38 PM (8 years, 5 months ago) |
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are you using agar?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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mezzy jeb
Skywalker


Registered: 07/28/12
Posts: 291
Last seen: 2 years, 4 months
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Y'all are wonderful. Ironically I had more success without a SAB than with, but considering I've only done about a dozen grows I'm not saying that proves anything, just that I've had interesting luck.
I've never used agar, but I'm considering it for my next grow. Anything you wanna tell me about it? I love the idea that you can cut away contaminated parts, and that you can potentially never have to buy a spore syringe/print again. But it also seems that mastery of sterile work is even more crucial with agar than with grains. Do y'all agree? And of course even with agar you have to get them onto grains at some point if you wanna bulk substrate. In my situation I'm thinking it may be better to increase my sterile work skills before taking the step to agar. But I also know plenty of you shroomerites will tell me to stop wasting time and get on it right away!
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Contamination central [Re: mezzy jeb]
#22108325 - 08/18/15 07:57 PM (8 years, 5 months ago) |
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Quote:
MajickMuffin said: Can someone give me a link for the agar shit?
Thanks guys
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Mad Season said:
Quote:
hiperhiper said: so what's the right procedure with grains? I've checked the teks and nobody said anything about this. I didn't know I was not supposed to use syringe on grains. What is cleaning up syringe on agar? what does it mean?
http://www.shroomery.org/forums/showflat.php/Number/18431006 http://www.shroomery.org/forums/showflat.php/Number/18430998 http://www.shroomery.org/forums/showflat.php/Number/19208976 http://www.shroomery.org/forums/showflat.php/Number/21889950/page/1
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mezzy jeb
Skywalker


Registered: 07/28/12
Posts: 291
Last seen: 2 years, 4 months
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Re: Contamination central [Re: Mad Season]
#22108353 - 08/18/15 08:04 PM (8 years, 5 months ago) |
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Thanks Mad Season. Man, I forgot that band existed! 
I've studied RR's videos intently, but it's always fun and beneficial to dive into more sources
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 20 hours, 14 minutes
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Re: Contamination central [Re: mezzy jeb]
#22108491 - 08/18/15 08:36 PM (8 years, 5 months ago) |
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Quote:
mezzy jeb said: Y'all are wonderful. Ironically I had more success without a SAB than with, but considering I've only done about a dozen grows I'm not saying that proves anything, just that I've had interesting luck.
I've never used agar, but I'm considering it for my next grow. Anything you wanna tell me about it? I love the idea that you can cut away contaminated parts, and that you can potentially never have to buy a spore syringe/print again. But it also seems that mastery of sterile work is even more crucial with agar than with grains. Do y'all agree? And of course even with agar you have to get them onto grains at some point if you wanna bulk substrate. In my situation I'm thinking it may be better to increase my sterile work skills before taking the step to agar. But I also know plenty of you shroomerites will tell me to stop wasting time and get on it right away!
You got it all wrong. You don't need to be more sterile to do agar work. Agar work simply lets you know when you've been less than sterile and allows a way to correct the problem before it winds up in your jars.
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