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Offlinepsilocybist
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My Subaeruginosa was actually a cubensis :S
    #22105583 - 08/18/15 07:07 AM (8 years, 5 months ago)

:cookiemonster:Just some images from my grow, no questions.
this images are the universe's respond to my prayers probably :dancer:

these are Psilocybe Subaeruginosa, showing germination and healthy mycelium growth, after giving up completely.

the jars were inoculated on July 22 , along other stuff, card-boards, and wood chips. (grains: corn, millet, wheat, each 2 jar, to be more sure about the mycelium taste and acceptance for germination :lol: obsession rules my mind, I know)
none of them showed germination signs after waiting for two weeks. and two weeks were enough for them to show mycelium. cause I used syringes for inoculation, so the spores were hydrated enough. (and none of jars/media showed contams.)
So I re-sterilized the jars, and once again, using the same syringe and spores, inoculated them on August 6 (last hope, final attempt)

after waiting for 7 days and no myc, thought the spores were not viable.
was going to toss the grains in jars away, and reuse them for another strain inoculation, but suddenly saw very little signs of fluffy white mycelium, barely visible.:laugh: real hope boost.

so after a day, and they became like this :

wheat jar :


corn jar :



And now, after a week (totally 13-14 days from inoc. date) :

millet jar :


Corn jar :


So the motto, Never give up on grows.


Edited by psilocybist (10/01/15 10:35 PM)


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Invisibleblindingleaf
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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22105604 - 08/18/15 07:18 AM (8 years, 5 months ago)

Quote:

So the motto, Never give up on grows.





QFT


--------------------
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MICROBIAL HUSBANDRY!!!!

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Offlinespacechildo
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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22105620 - 08/18/15 07:25 AM (8 years, 5 months ago)

cool stuff man! :cool::thumbup:


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Offlineblojo02184
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Re: Finally, Subaeruginosa mycelium :) [Re: blindingleaf]
    #22105623 - 08/18/15 07:26 AM (8 years, 5 months ago)

So.... Uhhhh....
You inoculated PS.Subaeruginosa into grain jars with a ms syringe...

Woah.

Luck comes in many forms.


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Invisibleblindingleaf
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Re: Finally, Subaeruginosa mycelium :) [Re: blojo02184]
    #22105628 - 08/18/15 07:27 AM (8 years, 5 months ago)

well, in all fairness, he did say
Quote:

this images are the universe's respond to my prayers probably




but ya, thats a dice roll for sure.


--------------------
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Offlinepsilocybist
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Re: Finally, Subaeruginosa mycelium :) [Re: blojo02184]
    #22105647 - 08/18/15 07:38 AM (8 years, 5 months ago)

Quote:

blojo02184 said:
So.... Uhhhh....
You inoculated PS.Subaeruginosa into grain jars with a ms syringe...

Woah.

Luck comes in many forms.




why? cause agar is the safest (or maybe the only) germination media for Subaeruginosa ?
if that's the case, i consider myself lucky :lol:


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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22106706 - 08/18/15 01:10 PM (8 years, 5 months ago)

:thumbup:


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OfflinetaGyo
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Re: Finally, Subaeruginosa mycelium :) [Re: newera]
    #22106816 - 08/18/15 01:46 PM (8 years, 5 months ago)

:popcorn:

Gonna keep this updated?


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Offlinepsilocybist
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Re: Finally, Subaeruginosa mycelium :) [Re: taGyo]
    #22106904 - 08/18/15 02:13 PM (8 years, 5 months ago)

Quote:

taGyo said:
:popcorn:

Gonna keep this updated?




abs-fuckin-lutely.

yesterday transferred a few grains to wet card-boards in petri dishes.
this is a close up of a wheat with myc, on the wet cardboard in petri :



and tomorrow I'm gonna go and look for wood chips.
there's plenty of cut-and-abandoned trees around city, so probably it's gonna be a hard day of turning big ass tree trunks into wood chips.

and since the summers hotness here last for a few next month, Do you think I can fruit them this year ?


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OfflinetaGyo
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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22106917 - 08/18/15 02:17 PM (8 years, 5 months ago)

:thumbup:


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Offlinepsilocybist
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Re: Finally, Subaeruginosa mycelium :) [Re: taGyo]
    #22110419 - 08/19/15 09:57 AM (8 years, 5 months ago)

And this is the same grain after finding out it's on cardboard and hugging it :


and his brothers on another cardboard :


btw, today found many dry trunks of wild olive (Elaeagnus angustifolia), gonna chop em off into chips tonight.
the wood is hard as stone !


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Offlinepsilocybist
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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22131167 - 08/23/15 02:36 PM (8 years, 5 months ago)

:cookiemonster: yesterday's pics, same petri.



I can see the daily difference in its growth over the petri  :awesome:

and yesterday I boiled around 3 Kg wood-chips for 1 hr , divided them into 3 parts, bagged them, and poured the colonized millet grains in bags and gave em a little shake. grains already show recovery after this transfer and have became fluffy again inside the bags.

medium woodchips size (more were smaller) :


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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22131276 - 08/23/15 03:05 PM (8 years, 5 months ago)

:kenthumbup:

Looks promising brotha!


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Re: Finally, Subaeruginosa mycelium :) [Re: taGyo]
    #22131917 - 08/23/15 05:13 PM (8 years, 5 months ago)

Good job so far. :popcorn:


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Offlinejsncrs
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Re: Finally, Subaeruginosa mycelium :) [Re: Grey]
    #22131969 - 08/23/15 05:26 PM (8 years, 5 months ago)

:threadmonitor:

Awesome pics man


Edited by jsncrs (08/23/15 05:26 PM)


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OfflineMandarinfish

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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22135028 - 08/24/15 11:07 AM (8 years, 5 months ago)

.


Edited by Mandarinfish (07/15/20 10:52 PM)


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Offlineivangrozny2
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Re: Finally, Subaeruginosa mycelium :) [Re: Mandarinfish]
    #22135552 - 08/24/15 01:50 PM (8 years, 5 months ago)

Mine germinated on agar in just a few days from a print, and was contam free, and remains so, it is now time for me to transfer them to grain, and then to wood.  It would be great if they fruited this year but I have my doubts about the possibility.


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Re: Finally, Subaeruginosa mycelium :) [Re: ivangrozny2]
    #22137286 - 08/24/15 07:55 PM (8 years, 5 months ago)

love a good woodlover grow, will be tuning in for sure :thumbup:


--------------------
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OfflineDarkhome
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Re: Finally, Subaeruginosa mycelium :) [Re: Coraltrout]
    #22137304 - 08/24/15 07:57 PM (8 years, 5 months ago)

Nice!:goodluck::threadmonitor:


--------------------
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"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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Offlinepsilocybist
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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22138704 - 08/25/15 03:52 AM (8 years, 5 months ago)

2 days later, same petri :
........

won't update this thread with petri pics anymore.(feels repetitive).
when the chips are colonized, will post the pics. (and hopefully, the outdoor patch and other stuff too)

BTW, do you know what are those concentric circles on myc around the grain ?
Is it because of day/night different lighting conditions, affecting the myc growth ?

love you all. :cookiemonster:


Edited by psilocybist (08/25/15 10:31 AM)


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OfflineNature Boy
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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22138785 - 08/25/15 04:40 AM (8 years, 5 months ago)

:thumbup:

Almost as sexy as pin-porn!

N.B.


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OfflinetaGyo
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Re: Finally, Subaeruginosa mycelium :) [Re: Nature Boy]
    #22139633 - 08/25/15 07:58 AM (8 years, 5 months ago)

Concentric circles?


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Offlinepsilocybist
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Re: Finally, Subaeruginosa mycelium :) [Re: taGyo]
    #22140218 - 08/25/15 10:34 AM (8 years, 5 months ago)

Quote:

taGyo said:
Concentric circles?




yeah, kinda.

Edited that post to make it more clear.
it's like one circle of Very-Rhizomic and one circle of Regular-Rhizomic and so on. :shrug:


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OfflinetaGyo
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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22140235 - 08/25/15 10:38 AM (8 years, 5 months ago)

Not sure but I'd transfer from 9 o'clock and 5 o'clock.


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Offlinespacechildo
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Re: Finally, Subaeruginosa mycelium :) [Re: taGyo]
    #22140441 - 08/25/15 11:28 AM (8 years, 5 months ago)

I see what you're talking about, if you dont focus your eyes on the first pic you can see it on the left side,
it looks a bit more grey and whispery and then normal white myc grows again.

i've heard some theories on it before but still wanna see what people say about that now :popcorn:


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Offlinepsilocybist
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Re: Finally, Subaeruginosa mycelium :) [Re: spacechildo]
    #22177043 - 09/02/15 05:04 AM (8 years, 4 months ago)

:cookiemonster:And here, it's been 11 days since I transferred colonized millet grains into wood chips buckets.
Just opened the cover of a bucket to see this wonderful stuff :



and the petris are almost covered with myc. gonna transfer them into wood-chips too. (in two days)

EDIT : yeah , I know, that's my hair :lol:


Edited by psilocybist (09/02/15 05:05 AM)


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Offlinepsilocybist
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My Subaeruginosa Grow-Log [Re: psilocybist]
    #22221196 - 09/11/15 10:18 AM (8 years, 4 months ago)

Yep, changed the thread title, seems more like a grow log so far.

Here's one of petri's :


Also, today I dumped my woodchips buckets and bags into a mini mono.

Almost fully colonized woodchips, dumped bucket :


some close ups :


Mixing colonized chips with non-colonized ones :


Final product :lol: :


gonna exposure them to natural conditions when the temps fall down, without making outdoor patches.
got around one month time till the real-autumn begins.
:cookiemonster:


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OfflinetaGyo
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Re: My Subaeruginosa Grow-Log [Re: psilocybist]
    #22221215 - 09/11/15 10:23 AM (8 years, 4 months ago)

Sounds good :dancer:


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Offlinebluelou
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Re: My Subaeruginosa Grow-Log [Re: taGyo]
    #22221783 - 09/11/15 12:34 PM (8 years, 4 months ago)

Great work so far!!!!

I've got some of these spores too,
so i am watching this one closely!

TEMPS are way high here also,sucks???

Wood beds are a dream really...
down south?

Good luck!


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Re: My Subaeruginosa Grow-Log [Re: bluelou]
    #22221843 - 09/11/15 12:48 PM (8 years, 4 months ago)

Lou, your spores are from Australia. It's pretty warm there too.


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Offlinepsilocybist
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Re: My Subaeruginosa Grow-Log [Re: Grey]
    #22259015 - 09/19/15 05:12 AM (8 years, 4 months ago)

:cookiemonster: the same tub above, after a week :


see the top layer chips ? they were mostly the non-colonized ones :eek:
And the temps have came down a little finally, now it's 75 days, 70-72 nights. :weirdeyes: don't know wtf is wrong with this year's summer, not showing its last days yet, though it's officially a fuckin autumn.
:aweshit: fuckin seasons, respect the calendar you bitches.


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Offlinepsilocybist
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Re: My Subaeruginosa Grow-Log [Re: psilocybist]
    #22272727 - 09/22/15 05:50 AM (8 years, 4 months ago)

Same above, after 3 days :


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Offlinepsilocybist
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Re: My Subaeruginosa Grow-Log [Re: psilocybist]
    #22306710 - 09/29/15 05:52 AM (8 years, 4 months ago)

WTF ? !!!! :confused::confused::confused::confused:
:confused::confused::confused::confused:

i'm confused as hell.
my tub pinned , found three little pins last night. wtf ?
can other cubes grow on wood-chips like this ?

I'm really doubting whether it was Subaeruginosa from start. :facepalm: :lol: :shrug:

are there any chances these pins be cubensis of any kind ?

of course not, they pinned without any decrease in temp, and no FAE, nothing at all.


:facepalm:  :doublefacepalm:  :huxleyfacepalm:  :smbfacepalm:  :facepalm3:  :stanhopefacepalm:
:shrug::confused::shrug:

EDIT : can't find enough facepalm smileys.


Edited by psilocybist (09/29/15 06:16 AM)


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Re: My Subaeruginosa Grow-Log [Re: psilocybist]
    #22320313 - 10/01/15 06:11 PM (8 years, 3 months ago)

who knows man just keep updating!!!!!!!!!!(the pic from it colonizing looks like cube myc


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Re: Finally, Subaeruginosa mycelium :) [Re: blojo02184]
    #22320365 - 10/01/15 06:25 PM (8 years, 3 months ago)

Quote:

blojo02184 said:
So.... Uhhhh....
You inoculated PS.Subaeruginosa into grain jars with a ms syringe...

Woah.

Luck comes in many forms.



dude a couple years ago i did azurs stuntzii baeo's libs allenii and a wild cube and they all did great:crazy:


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Re: Finally, Subaeruginosa mycelium :) [Re: psilocybist]
    #22320372 - 10/01/15 06:27 PM (8 years, 3 months ago)

Quote:

psilocybist said:
Quote:

taGyo said:
Concentric circles?




yeah, kinda.

Edited that post to make it more clear.
it's like one circle of Very-Rhizomic and one circle of Regular-Rhizomic and so on. :shrug:



yes that is a day/night thing


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Re: Finally, Subaeruginosa mycelium :) [Re: cronicr]
    #22320708 - 10/01/15 07:26 PM (8 years, 3 months ago)

What do you mean by day/night thing Cron?


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Re: Finally, Subaeruginosa mycelium :) [Re: taGyo]
    #22320913 - 10/01/15 08:07 PM (8 years, 3 months ago)

Rings in the agar


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Re: Finally, Subaeruginosa mycelium :) [Re: cronicr]
    #22321036 - 10/01/15 08:30 PM (8 years, 3 months ago)

Oh I thought you meant the day and night schedule affected getting rings on agar :laugh2:


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TNF Q&A :rockon:
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Offlinepsilocybist
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Re: My Subaeruginosa Grow-Log [Re: newera]
    #22321516 - 10/01/15 10:33 PM (8 years, 3 months ago)

Quote:

newera said:
who knows man just keep updating!!!!!!!!!!(the pic from it colonizing looks like cube myc




nope, now I'm sure it's a cube of some unknown strain.
All this effort :facepalm:

they pinned without any FAE, and no temp fall, so it's not Subaeruginosa for sure.
it was a freebie print labeled as Subaeruginosa , sent to me along others, so no complain.

So, now I have some cube myc, colonizing wood chips, being fruited in a tub. :lol:


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Re: My Subaeruginosa Grow-Log [Re: psilocybist]
    #22321807 - 10/02/15 12:16 AM (8 years, 3 months ago)

:lol:cubes on wood chips...good stuff:hehehe:


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Re: My Subaeruginosa Grow-Log [Re: cronicr]
    #22322466 - 10/02/15 07:47 AM (8 years, 3 months ago)

:laugh2:

I was pretty ready for this one :/, I have these prints on the back burner for next spring until I can do a nice outdoor woodchip bed.


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Re: My Subaeruginosa Grow-Log [Re: taGyo]
    #22322582 - 10/02/15 08:27 AM (8 years, 3 months ago)

Same here. I've got a really nice print, but never got around to it.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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OfflinetaGyo
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Re: My Subaeruginosa Grow-Log [Re: Grey]
    #22322599 - 10/02/15 08:32 AM (8 years, 3 months ago)

Grow them next spring with me if you got an outdoor spot, I think they'll thrive out there bro. I've been doing a lot of research on what they like and would be happy to share :super:


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Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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InvisibleGrey
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Re: My Subaeruginosa Grow-Log [Re: taGyo]
    #22322641 - 10/02/15 08:48 AM (8 years, 3 months ago)

I plan on it man. I want a lot of spawn ready.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


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OfflinetaGyo
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Re: My Subaeruginosa Grow-Log [Re: Grey]
    #22322667 - 10/02/15 08:59 AM (8 years, 3 months ago)

See you when the leaves come back :rockon:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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Offlinepsilocybist
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Re: My Subaeruginosa Grow-Log [Re: taGyo]
    #22335175 - 10/04/15 11:00 PM (8 years, 3 months ago)

and here's the last pic of this thread.



:cookiemonster:


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OfflinecronicrFacebook
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Re: My Subaeruginosa Grow-Log [Re: psilocybist]
    #22335271 - 10/04/15 11:37 PM (8 years, 3 months ago)

:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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