|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
|
Finding a Contaminant That may or may Not exist! ?
#22104995 - 08/18/15 12:01 AM (8 years, 5 months ago) |
|
|
Hey peeps.
I have been working with this new species found in the sf bay area. Been getting prints from wild samples and starting them on agar. I almost never get any noticeable contams on agar. I usually transfer at least twice away from the spore master before I will use it. So my potential problem is grain spawn. It never grows as well as I would like to see on grain. It never stinks, it smells like mushroom mycelium. The only negative things that I see is a slow response to recover after shaking a fully colonized jar and the density. I always blamed it on the species. But currently comparing it to another wood loving species makes me second guess it again.
How do I find something that may or may Not be there? I just got some antibiotic agar and will use it from spores to finish. Hopefully I see better results when it comes to grain.
Here is a pick with what I am working with on the left and a similar wood lover on the right. The one on the right was a multi spore syringe from wild part. The left is from a wild print germinated on agar and transferred to the bag once ready.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: tahoe]
#22105055 - 08/18/15 12:31 AM (8 years, 5 months ago) |
|
|
the easiest answer...try and fruit it cool stuff though tahoe
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: cronicr]
#22105176 - 08/18/15 01:59 AM (8 years, 5 months ago) |
|
|
I like the look of your agar dish. The bacteria has to either be coming in during the transfer, or the grains need to be sterilized more. Up the pressure or up the pc times to kill those shabby endospores.
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: Mad Season]
#22105329 - 08/18/15 04:01 AM (8 years, 5 months ago) |
|
|
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
keeno
enthusiast



Registered: 06/01/11
Posts: 2,679
Loc: UK
Last seen: 14 days, 5 hours
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: blindingleaf]
#22105444 - 08/18/15 05:36 AM (8 years, 5 months ago) |
|
|
I've only worked with Allenii, and I found that it had pretty weak myc on grain compared to cubes 
might just be it's thing?

this one was ready to go and seems pretty happy in its wood chip bed

sorry I couldn't be more helpful
-------------------- Check out my Psilocybe Cyanofriscosa (Allenii) TEK Top fungi-knowlodgy with THE TRIBE! THE TRIBE
|
tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: keeno] 1
#22105576 - 08/18/15 07:04 AM (8 years, 5 months ago) |
|
|
Keeno. That is extremely helpful. ;-)
I have noticed that alleni is super aggressive On wood. It is taking over in these parts.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
|
blojo02184
Big Red



Registered: 05/15/13
Posts: 3,525
Loc: Maine
Last seen: 10 months, 3 days
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: tahoe]
#22105602 - 08/18/15 07:16 AM (8 years, 5 months ago) |
|
|
I dunno what species it is... You were vague, other than it is a wood decomposer.
When I did azures I noticed agar myc looked fluffy in some parts and flat in others, like it was unsure what to do. Then the grain jars were pretty wispy, definitely did not like the shake. Once I put it to wood chips and sawdust it took off.
Here's the weird part. I thought the plates were dirty, but went ahead anyways. The jars didn't show mold, but they were "juicy" and not in a good way. No horrible smells, but still juicy. Then when it was colonizing the wood chips and sawdust it developed a funk. So I knew there was bacteria. Then a small patch of green developed on a couple small sticks in the bucket.
At that point, 2 months into it, I said fuck it and prepped a bed and buried that sucker. It fruited later that year and still kicking alive today. Gotta wait til Halloween to get more, that's about when they fruit in my area.
Don't know if your going outdoors with your project, but if you feel wary of it, just go for it.
|
tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: blojo02184] 1
#22106991 - 08/18/15 02:38 PM (8 years, 5 months ago) |
|
|
Blojo, I will use the spawn as long as it's growing. Weiss lovers can beat competitors once on wood. I have seen it done many times. I bury all the spawn in local parking lots, etc. I get new patches all the time.
I am more concerned with this grow because is getting buried in my landscaping.
The species is alleni on the left, azure I the right.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: tahoe]
#22107477 - 08/18/15 04:33 PM (8 years, 5 months ago) |
|
|
my allenii puts some metabs out on grain when it over colonizes and when the temps get really warm
 not sure yuo can see much there here it is four months ago
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: cronicr]
#22108252 - 08/18/15 07:36 PM (8 years, 5 months ago) |
|
|
Cron. That is the exact same growth that I get from alleni. Cool. Makes me feel better.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: tahoe]
#22108288 - 08/18/15 07:45 PM (8 years, 5 months ago) |
|
|
good luck man i just cased mine!
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: cronicr]
#22109474 - 08/19/15 01:47 AM (8 years, 5 months ago) |
|
|
Quote:
cronicr said:
good luck man i just cased mine!
Me too! Well wouldn't say cased. But here they are.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: tahoe]
#22109491 - 08/19/15 02:01 AM (8 years, 5 months ago) |
|
|
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
keeno
enthusiast



Registered: 06/01/11
Posts: 2,679
Loc: UK
Last seen: 14 days, 5 hours
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: cronicr]
#22109553 - 08/19/15 02:37 AM (8 years, 5 months ago) |
|
|
holy shit!!! now that's a bed!!

yeah I got quite a lot of metabolites on mine too
 
I just thought it was cause I had shonky sterile techniques
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: keeno] 1
#22109561 - 08/19/15 02:42 AM (8 years, 5 months ago) |
|
|
 i gave my bed some trees
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
keeno
enthusiast



Registered: 06/01/11
Posts: 2,679
Loc: UK
Last seen: 14 days, 5 hours
|
Re: Finding a Contaminant That may or may Not exist! ? [Re: cronicr]
#22109582 - 08/19/15 03:03 AM (8 years, 5 months ago) |
|
|
good move 
|
|