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Offlinespacechildo
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Re: Fruiting bag in vitro [Re: dirttoldfart]
    #22101918 - 08/17/15 09:00 AM (8 years, 5 months ago)

its the white myc itself. the texture is off. classic bacteria IMO,
once you see healthy myc you can tell there's a big difference in apperance,
it has nothing to do with whether there is some manure not pictured somewhere in the bag..


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InvisibleKauaiOrca
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Re: Fruiting bag in vitro [Re: spacechildo]
    #22101957 - 08/17/15 09:15 AM (8 years, 5 months ago)

I've grown a lot of grain bags and that growth looks fine to me.  I wouldn't even consider tossing it.  Might be the glare off the reflection of the bag creating some optical distortion?

Don't worry too much about it ... let it keep growing.  Do you have a pressure cooker?  If so, you can easily test whether or not your spore solution was clean or not.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Offlinedirttoldfart
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Re: Fruiting bag in vitro [Re: KauaiOrca]
    #22101978 - 08/17/15 09:20 AM (8 years, 5 months ago)

Nope, no pressure cooker. That's the reason I bought this bag instead of just continuing to try to do everything through steam sterilization


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InvisibleKauaiOrca
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Re: Fruiting bag in vitro [Re: dirttoldfart]
    #22101993 - 08/17/15 09:26 AM (8 years, 5 months ago)

Quote:

dirttoldfart said:
Nope, no pressure cooker. That's the reason I bought this bag instead of just continuing to try to do everything through steam sterilization




Get yourself a cheap pressure cooker ... used one maybe at a garage sale or goodwill ... it opens up a ton of much better possibilities.  Will pay for itself very quickly.  Your costs go way, way down when you get a pressure cooker.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Offlinespacechildo
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Re: Fruiting bag in vitro [Re: dirttoldfart]
    #22102050 - 08/17/15 09:49 AM (8 years, 5 months ago)

Quote:

dirttoldfart said:
Nope, no pressure cooker. That's the reason I bought this bag instead of just continuing to try to do everything through steam sterilization




pre-made bags like that fails so many times dude, I see it almost daily here.
also the poke hole in bag and hurry up and tape the hole TEK is really old, there's so many better ways to do it.

If you wanna do grains you should buy a PC, otherwise just make cakes and use them as spawn.


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Offlinedirttoldfart
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Re: Fruiting bag in vitro [Re: spacechildo]
    #22102095 - 08/17/15 10:05 AM (8 years, 5 months ago)

This bag actually has a self-healing injection port. I guess I was just a little paranoid about contams from my failed attempt at BRF cakes, so I put tape over the injection port after I inoculated.


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InvisibleKauaiOrca
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Re: Fruiting bag in vitro [Re: dirttoldfart]
    #22102146 - 08/17/15 10:15 AM (8 years, 5 months ago)

Quote:

dirttoldfart said:
This bag actually has a self-healing injection port. I guess I was just a little paranoid about contams from my failed attempt at BRF cakes, so I put tape over the injection port after I inoculated.




Inoculating grain bags with a vendor spore syringe definitely has a lot of risks because the growth from a multi spore syringe is so slow that it gives other contaminants that might get in there (often in the syringe itself) a lot of time to get a foothold and take over.  One of the most important lessons I learned is SPEED.  To give the Mycelium as much of a head start on possible contaminants as you possibly can.  Inoculating with a strong healthy and clean LC or LI will colonize the grain in a week as opposed to 4-8 weeks (or more) with multi spore syringe.  This allows the mycelium to win the race for resources as opposed to giving contaminants a head start. 

A pressure cooker gives you a pathway to get a really clean inoculant and then to get the upper hand on speed.  It is also then really cheap to prepare grain bags or jars.  I just bought a 50 lb bag of grass seed for $23 which is enough for years of growing at the pace I do it. 

Do you have any more spore solution in your syringe or did you use it all up?


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


Edited by KauaiOrca (08/17/15 10:16 AM)


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Offlinespacechildo
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Re: Fruiting bag in vitro [Re: dirttoldfart]
    #22102170 - 08/17/15 10:19 AM (8 years, 5 months ago)

for the best chance of success I would re-try the PF-tek. You already got all you need for it, jars,brf,verm,and a pot,
just follow www.mushroomvideos.com and do exactly like he does, if they grow
mold on you post a pic and maybe the syringe is to blame! not your fault if the syringes are dirty!


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Offlinedirttoldfart
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Re: Fruiting bag in vitro [Re: KauaiOrca]
    #22102221 - 08/17/15 10:30 AM (8 years, 5 months ago)

There's still about 10 mL left in the syringe, out of 20


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InvisiblePirateSwazey
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Re: Fruiting bag in vitro [Re: spacechildo]
    #22102244 - 08/17/15 10:36 AM (8 years, 5 months ago)

I'd just like to add to this thread that there are some misconceptions about bulk spawning going on here.. A coir sub is no more prone to contamination than a PF tek, 'mudabottle', or straight cased grains. It all boils down to how you inoculated the spawn -- if you used a syringe it is going to have the same chance of failure no matter how you choose to fruit!

When I started growing mushrooms years ago I began by using bird seed and coir subs; it wasn't until sometime last year I made my first cakes... I honestly felt like the cakes were more difficult to take care of than a bulk tub that takes care of itself! As for casing grains - that requires properly pasteurizing, which is also more difficult than tossing boiling water into a bucket.

A bulk substrate is going to make about 4-8 ounces of mushrooms, which may be more than a lot of people need... but hey, you can always save them for later, or give some free ounces to your friends so they can share with their friends. Be creative and make some chocolates or something - those will last in the freezer for a long time. If you don't need a lot then that one flush will last you a really long time, and that means less frequency of ordering syringes, grain bags, or whatever.

:peace:


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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InvisibleKauaiOrca
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Re: Fruiting bag in vitro [Re: dirttoldfart]
    #22102257 - 08/17/15 10:40 AM (8 years, 5 months ago)

Quote:

dirttoldfart said:
There's still about 10 mL left in the syringe, out of 20




If you want to have a lot more fun with this hobby, go get yourself a cheap pressure cooker and consider this very easy and simple tek.  You can use it to grow indefinitely and as a springboard to bigger and better growing teks as you learn more.  This Tek works equally well with BRF/Verm or Grass Seed, which I prefer a lot more.  It can be used to create a Grain Liquid Culture too which will eliminate your need to buy other spore syringes.  With 10 ML's you could easily start 5 jars growing and they'll be fruiting way before that bag does.

http://www.shroomery.org/forums/showflat.php/Number/21390984

This Tek +  SGFC and you're going to get good results.  There are other GREAT teks too just showing this one because I found it incredibly easy to follow and it worked perfectly for me.


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


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Offlinespacechildo
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Re: Fruiting bag in vitro [Re: PirateSwazey]
    #22102260 - 08/17/15 10:41 AM (8 years, 5 months ago)

Quote:

PirateSwazey said:
I'd just like to add to this thread that there are some misconceptions about bulk spawning going on here.. A coir sub is no more prone to contamination than a PF tek, 'mudabottle', or straight cased grains. It all boils down to how you inoculated the spawn -- if you used a syringe it is going to have the same chance of failure no matter how you choose to fruit!
:peace:




completely agree!
that is if you make everything yourself, if OP is gonna buy another bag I'd say he has better chances doing it himself
but since he dont have a PC he should just do cakes IMO.

clean spawn is all that matters, and it sure matters a lot!


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InvisibleKauaiOrca
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Re: Fruiting bag in vitro [Re: spacechildo]
    #22102266 - 08/17/15 10:43 AM (8 years, 5 months ago)

Quote:

spacechildo said:

clean spawn is all that matters, and it sure matters a lot!




That is for SURE!!!!


--------------------
"The universe is endless, limitless and infinite.  Any effort to define it's boundaries is an attempt to overcome ignorance.  We are physical, mental and spiritual beings ... there is no beginning and there is no end.  There is only memory.  Our repeated loss of memory experiences create the illusion of beginnings and ends.  Immortality is the ability to retain full memory through all consciousness transformations.  Loss of memory is man's greatest curse and, in very real terms, death."

-- Ancient Taoist Master


Edited by KauaiOrca (08/17/15 10:45 AM)


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Offlinespacechildo
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Re: Fruiting bag in vitro [Re: spacechildo]
    #22102275 - 08/17/15 10:45 AM (8 years, 5 months ago)

:nodofunderstanding:

but it will always be easier to tell yourself it was the bucket tek'd coir :wink:


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InvisiblePirateSwazey
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Re: Fruiting bag in vitro [Re: spacechildo]
    #22102312 - 08/17/15 10:53 AM (8 years, 5 months ago)

I think it's fine to order a grain bag... hell, a lot of commercial mushroom farms sub out their entire spawn production. The problem with pre made bags is what people put into them and how they do it. Personally I have made enough grain bags that I would feel comfortable selling them to people on the internet knowing they would be fine to use even months after I made them but it's just not what I want to do.

Point being...  again...  clean inoculant! :smirk:


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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Invisibleblindingleaf
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Re: Fruiting bag in vitro [Re: PirateSwazey]
    #22102530 - 08/17/15 12:00 PM (8 years, 5 months ago)

Quote:

Personally I have made enough grain bags that I would feel comfortable selling them to people on the internet




^^^i'll beat his price by half!!

we owned by the same company, yo :wink:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisiblePirateSwazey
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Re: Fruiting bag in vitro [Re: blindingleaf]
    #22102789 - 08/17/15 01:16 PM (8 years, 5 months ago)

Dude don't shoot us in the foot!


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



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